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(R)-gamma-caprolactone + H2O
4-hydroxycaproate
-
-
-
?
(S)-gamma-caprolactone + H2O
caproate
-
-
-
?
3-oxo-dodecanoyl-L-homoserine lactone + H2O
3-oxo-dodecanoyl-L-homoserine
-
-
-
?
5-thiobutyl butyrolactone + H2O
?
TBBL
-
-
?
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
delta valerolactone + H2O
valerate
-
-
-
?
delta-decalactone + H2O
5-hydroxydecanoate
-
-
-
?
delta-decalactone + H2O
5-hydroxydecanoic acid
-
-
-
?
delta-dodecalactone + H2O
5-hydroxydodecanoic acid
-
-
-
?
delta-nonalactone + H2O
5-hydroxynonanoate
delta-nonalactone + H2O
5-hydroxynonanoic acid
delta-undecalactone + H2O
5-hydroxyundecanoic acid
-
-
-
?
delta-valerolactone + H2O
5-hydroxypentanoic acid
dodecanoic-delta-lactone + H2O
5-hydroxydodecanoate
-
-
-
?
dodecanoic-gamma-lactone + H2O
dodecanoate
-
-
-
?
epsilon-caprolactone + H2O
6-hydroxycaproate
-
-
-
?
ethyl paraoxon + H2O
4-nitrophenol + diethyl phosphate
-
-
-
-
?
gamma-butyrolactone + H2O
4-hydroxybutanoic acid
gamma-caprolactone + H2O
4-hydroxyhexanoic acid
-
-
-
?
gamma-dodecalactone + H2O
4-hydroxydodecanoic acid
-
-
-
?
gamma-heptalactone + H2O
4-hydroxyheptanoic acid
-
-
-
?
gamma-heptanolide + H2O
4-hydroxyheptanoic acid
-
-
-
?
gamma-heptanolide + H2O
?
-
-
-
?
gamma-nonalactone + H2O
4-hydroxynonanoate
gamma-nonalactone + H2O
4-hydroxynonanoic acid
gamma-undecalactone + H2O
4-hydroxyundecanoic acid
-
-
-
?
heptanoic-gamma-lactone + H2O
4-hydroxyheptanoate
-
-
-
?
homo-L-cysteine thiolactone + H2O
homo-L-cysteine
L-homoserine lactone + H2O
L-homoserine
N-(3-hydroxy-dodecanoyl)-L-homoserine lactone + H2O
N-(3-hydroxy-dodecanoyl)-L-homoserine
relative activity: 91.9%
-
-
?
N-(3-hydroxybutyryl)-L-homoserine lactone + H2O
N-(3-hydroxybutyryl)-L-homoserine
-
-
-
?
N-(3-hydroxydodecanoyl)-L-homoserine lactone + H2O
N-(3-hydroxydodecanoyl)-L-homoserine
-
-
-
?
N-(3-hydroxyhexanoyl)-L-homoserine lactone + H2O
N-(3-hydroxyhexanoyl)-L-homoserine
N-(3-hydroxyoctanoyl)-DL-homoserine lactone + H2O
N-(3-hydroxyoctanoyl)-L-homoserine
-
-
-
-
?
N-(3-hydroxyoctanoyl)-L-homoserine lactone + H2O
N-(3-hydroxyoctanoyl)-L-homoserine
-
-
-
?
N-(3-hydroxytetradecanoyl)-L-homoserine lactone + H2O
N-(3-hydroxytetradecanoyl)-L-homoserine
-
-
-
?
N-(3-oxo-decanoyl)-L-homoserine lactone + H2O
N-(3-oxo-decanoyl)-L-homoserine
N-(3-oxo-dodecanoyl)-L-homoserine lactone + H2O
N-(3-oxo-dodecanoyl)-L-homoserine
relative activity: 83.3%
-
-
?
N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O
N-(3-oxo-N-hexanoyl)-L-homoserine
N-(3-oxo-octanoyl)-L-homoserine lactone + H2O
N-(3-oxo-octanoyl)-L-homoserine
N-(3-oxo-tetradecanoyl)-L-homoserine lactone + H2O
N-(3-oxo-tetradecanoyl)-L-homoserine
relative activity: 95.4%
-
-
?
N-(3-oxobutyryl)-L-homoserine lactone + H2O
N-(3-oxobutyryl)-L-homoserine
-
-
-
?
N-(3-oxodecanoyl)-L-homoserine lactone + H2O
N-(3-oxodecanoyl)-L-homoserine
N-(3-oxodecyl)-L-homoserine lactone + H2O
N-(3-oxodecyl)-L-homoserine
N-(3-oxododecanoyl)-L-homoserine lactone + H2O
N-(3-oxododecanoyl)-L-homoserine
N-(3-oxododecyl)-L-homoserine lactone + H2O
N-(3-oxododecyl)-L-homoserine
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
L-homoserine lactone + 3-oxohexanoate
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
N-(3-oxooctanoyl)-L-homoserine lactone + H2O
L-homoserine lactone + 3-oxooctanoate
-
-
-
?
N-(3-oxooctanoyl)-L-homoserine lactone + H2O
N-(3-oxooctanoyl)-L-homoserine
N-(3-oxooctyl)-L-homoserine lactone + H2O
N-(3-oxooctyl)-L-homoserine
N-(3-oxotetradecanoyl)-L-homoserine lactone + H2O
N-(3-oxotetradecanoyl)-L-homoserine
-
-
-
?
N-3-hydroxybutanoyl-L-homoserine lactone + H2O
N-3-hydroxybutanoyl-L-homoserine
-
-
-
-
?
N-3-oxo-decanoyl-DL-homoserine lactone + H2O
N-3-oxo-decanoyl-DL-homoserine
-
-
-
?
N-3-oxo-dodecanoyl-L-homoserine lactone + H2O
N-3-oxo-dodecanoyl-L-homoserine
N-3-oxo-hexanoyl-L-homoserine lactone + H2O
N-3-oxo-hexanoyl-L-homoserine
N-3-oxo-octanoyl-DL-homoserine lactone + H2O
N-3-oxo-octanoyl-DL-homoserine
-
-
-
?
N-3-oxo-octanoyl-L-homoserine lactone + H2O
N-3-oxo-octanoyl-L-homoserine
N-3-oxobutanoyl-L-homoserine lactone + H2O
N-3-oxobutanoyl-L-homoserine
-
-
-
-
?
N-3-oxodecanoyl-DL-homoserine lactone + H2O
N-3-oxodecanoyl-DL-homoserine
the N-oxodecanoyl-DL-homoserine lactone-hydrolyzing activity of recombinant h-PON1 enzyme is determined by using a recombinant quorum-sensing reporter Escherichia coli strain
-
-
?
N-3-oxodecanoyl-L-homoserine lactone + H2O
N-3-oxodecanoyl-L-homoserine
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
N-butanoyl-DL-homoserine lactone + H2O
N-butanoyl-DL-homoserine
N-butanoyl-L-homoserine lactone + H2O
N-butanoyl-L-homoserine
N-butyryl-DL-homoserine lactone + H2O
N-butyryl-DL-homoserine
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
N-decanoyl-L-homoserine lactone + H2O
L-homoserine lactone + decanoate
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
N-dodecanoyl-L-homoserine lactone + H2O
L-homoserine lactone + dodecanoate
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
N-heptanoyl-DL-homoserine lactone + H2O
N-heptanoyl-DL-homoserine
-
relative activity: 73%
-
-
?
N-heptanoyl-L-homoserine lactone + H2O
N-heptanoyl-L-homoserine
N-hexanoyl-(S)-homoserine lactone
N-hexanoyl-(S)-homoserine
N-hexanoyl-(S)-homoserine lactone + H2O
N-hexanoyl-(S)-homoserine
N-hexanoyl-DL-homoserine lactone + H2O
N-hexanoyl-DL-homoserine
-
relative activity: 100%
-
-
?
N-hexanoyl-DL-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
L-homoserine lactone + hexanoate
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
N-hexanoyl-L-homoserine thiolactone + H2O
?
structures of the lactonase bound with the ring-opened product of N-hexanoyl-L-homoserine thiolactone, overview
-
-
?
N-hexyl-L-homoserine lactone + H2O
N-hexyl-L-homoserine
N-octanoyl-DL-homoserine lactone + H2O
N-octanoyl-DL-homoserine
N-octanoyl-DL-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
L-homoserine lactone + octanoate
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
N-pentanoyl-L-homoserine lactone + H2O
N-pentanoyl-L-homoserine
N-tetradecanoyl-L-homoserine lactone + H2O
N-tetradecanoyl-L-homoserine
nonanoic-delta-lactone + H2O
5-hydroxynonanoate
-
-
-
?
nonanoic-delta-lactone + H2O
5-hydroxynonanoic acid
-
-
-
?
nonanoic-gamma-lactone + H2O
4-hydroxynonanoate
best substrate
-
-
?
nonanoic-gamma-lactone + H2O
4-hydroxynonanoic acid
best substrate
-
-
?
paraoxon + H2O
diethylphosphate + 4-nitrophenol
-
low activity
-
-
?
rac-gamma-caprolactone + H2O
4-hydroxycaproate
-
-
-
?
undecanoic-delta-lactone + H2O
5-hydroxyundecanoate
-
-
-
?
undecanoic-delta-lactone + H2O
5-hydroxyundecanoic acid
-
-
-
?
undecanoic-gamma-lactone + H2O
4-hydroxyundecanoate
-
-
-
?
undecanoic-gamma-lactone + H2O
4-hydroxyundecanoic acid
-
-
-
?
additional information
?
-
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
-
-
-
?
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
-
-
-
?
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
-
-
-
-
?
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
-
-
-
?
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
Tritonibacter mobilis
-
-
-
-
?
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
Tritonibacter mobilis YJ3
-
-
-
-
?
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
-
-
-
?
delta-nonalactone + H2O
5-hydroxynonanoate
-
-
-
-
?
delta-nonalactone + H2O
5-hydroxynonanoate
-
high activity
-
-
?
delta-nonalactone + H2O
5-hydroxynonanoic acid
-
-
-
?
delta-nonalactone + H2O
5-hydroxynonanoic acid
-
-
-
?
delta-valerolactone + H2O
5-hydroxypentanoic acid
-
-
-
?
delta-valerolactone + H2O
5-hydroxypentanoic acid
-
-
-
?
gamma-butyrolactone + H2O
4-hydroxybutanoic acid
-
-
-
?
gamma-butyrolactone + H2O
4-hydroxybutanoic acid
-
-
-
?
gamma-nonalactone + H2O
4-hydroxynonanoate
-
-
-
-
?
gamma-nonalactone + H2O
4-hydroxynonanoate
-
high activity
-
-
?
gamma-nonalactone + H2O
4-hydroxynonanoic acid
-
-
-
?
gamma-nonalactone + H2O
4-hydroxynonanoic acid
-
-
-
?
homo-L-cysteine thiolactone + H2O
homo-L-cysteine
-
-
-
?
homo-L-cysteine thiolactone + H2O
homo-L-cysteine
-
-
-
?
L-homoserine lactone + H2O
L-homoserine
-
-
-
-
?
L-homoserine lactone + H2O
L-homoserine
20% of the activity with N-decanoyl-L-homoserine lactone
-
-
?
L-homoserine lactone + H2O
L-homoserine
20% of the activity with N-decanoyl-L-homoserine lactone
-
-
?
L-homoserine lactone + H2O
L-homoserine
-
-
-
-
?
L-homoserine lactone + H2O
L-homoserine
-
-
-
-
?
N-(3-hydroxyhexanoyl)-L-homoserine lactone + H2O
N-(3-hydroxyhexanoyl)-L-homoserine
-
-
-
?
N-(3-hydroxyhexanoyl)-L-homoserine lactone + H2O
N-(3-hydroxyhexanoyl)-L-homoserine
-
-
-
-
?
N-(3-hydroxyhexanoyl)-L-homoserine lactone + H2O
N-(3-hydroxyhexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxo-decanoyl)-L-homoserine lactone + H2O
N-(3-oxo-decanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxo-decanoyl)-L-homoserine lactone + H2O
N-(3-oxo-decanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxo-decanoyl)-L-homoserine lactone + H2O
N-(3-oxo-decanoyl)-L-homoserine
relative activity: 97.3%
-
-
?
N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O
N-(3-oxo-N-hexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O
N-(3-oxo-N-hexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O
N-(3-oxo-N-hexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O
N-(3-oxo-N-hexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O
N-(3-oxo-N-hexanoyl)-L-homoserine
relative activity: 97.2%
-
-
?
N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O
N-(3-oxo-N-hexanoyl)-L-homoserine
-
relative activity: 97.2%
-
-
?
N-(3-oxo-N-hexanoyl)-L-homoserine lactone + H2O
N-(3-oxo-N-hexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxo-octanoyl)-L-homoserine lactone + H2O
N-(3-oxo-octanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxo-octanoyl)-L-homoserine lactone + H2O
N-(3-oxo-octanoyl)-L-homoserine
relative activity: 100%
-
-
?
N-(3-oxo-octanoyl)-L-homoserine lactone + H2O
N-(3-oxo-octanoyl)-L-homoserine
-
relative activity: 100%
-
-
?
N-(3-oxodecanoyl)-L-homoserine lactone + H2O
N-(3-oxodecanoyl)-L-homoserine
-
-
-
?
N-(3-oxodecanoyl)-L-homoserine lactone + H2O
N-(3-oxodecanoyl)-L-homoserine
the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms
-
-
?
N-(3-oxodecanoyl)-L-homoserine lactone + H2O
N-(3-oxodecanoyl)-L-homoserine
the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms
-
-
?
N-(3-oxodecyl)-L-homoserine lactone + H2O
N-(3-oxodecyl)-L-homoserine
-
-
-
-
?
N-(3-oxodecyl)-L-homoserine lactone + H2O
N-(3-oxodecyl)-L-homoserine
-
-
-
-
?
N-(3-oxodecyl)-L-homoserine lactone + H2O
N-(3-oxodecyl)-L-homoserine
-
-
-
-
?
N-(3-oxododecanoyl)-L-homoserine lactone + H2O
N-(3-oxododecanoyl)-L-homoserine
-
-
-
?
N-(3-oxododecanoyl)-L-homoserine lactone + H2O
N-(3-oxododecanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxododecanoyl)-L-homoserine lactone + H2O
N-(3-oxododecanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxododecanoyl)-L-homoserine lactone + H2O
N-(3-oxododecanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxododecanoyl)-L-homoserine lactone + H2O
N-(3-oxododecanoyl)-L-homoserine
the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms
-
-
?
N-(3-oxododecanoyl)-L-homoserine lactone + H2O
N-(3-oxododecanoyl)-L-homoserine
the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms
-
-
?
N-(3-oxododecyl)-L-homoserine lactone + H2O
N-(3-oxododecyl)-L-homoserine
-
-
-
-
?
N-(3-oxododecyl)-L-homoserine lactone + H2O
N-(3-oxododecyl)-L-homoserine
-
-
-
-
?
N-(3-oxododecyl)-L-homoserine lactone + H2O
N-(3-oxododecyl)-L-homoserine
-
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
L-homoserine lactone + 3-oxohexanoate
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
L-homoserine lactone + 3-oxohexanoate
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
product identification by mass spectrometry
-
-
r
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms
-
-
?
N-(3-oxooctanoyl)-L-homoserine lactone + H2O
N-(3-oxooctanoyl)-L-homoserine
-
-
-
?
N-(3-oxooctanoyl)-L-homoserine lactone + H2O
N-(3-oxooctanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxooctanoyl)-L-homoserine lactone + H2O
N-(3-oxooctanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxooctanoyl)-L-homoserine lactone + H2O
N-(3-oxooctanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxooctanoyl)-L-homoserine lactone + H2O
N-(3-oxooctanoyl)-L-homoserine
the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms
-
-
?
N-(3-oxooctanoyl)-L-homoserine lactone + H2O
N-(3-oxooctanoyl)-L-homoserine
the enzyme has a preference for N-acyl homoserine lactones with acyl chain lengths of at least 8 carbon atoms
-
-
?
N-(3-oxooctyl)-L-homoserine lactone + H2O
N-(3-oxooctyl)-L-homoserine
-
-
-
-
?
N-(3-oxooctyl)-L-homoserine lactone + H2O
N-(3-oxooctyl)-L-homoserine
-
-
-
-
?
N-(3-oxooctyl)-L-homoserine lactone + H2O
N-(3-oxooctyl)-L-homoserine
-
-
-
-
?
N-3-oxo-dodecanoyl-L-homoserine lactone + H2O
N-3-oxo-dodecanoyl-L-homoserine
-
-
-
-
?
N-3-oxo-dodecanoyl-L-homoserine lactone + H2O
N-3-oxo-dodecanoyl-L-homoserine
-
-
-
-
?
N-3-oxo-hexanoyl-L-homoserine lactone + H2O
N-3-oxo-hexanoyl-L-homoserine
-
-
-
-
?
N-3-oxo-hexanoyl-L-homoserine lactone + H2O
N-3-oxo-hexanoyl-L-homoserine
-
lower activity
-
-
?
N-3-oxo-octanoyl-L-homoserine lactone + H2O
N-3-oxo-octanoyl-L-homoserine
-
-
-
-
?
N-3-oxo-octanoyl-L-homoserine lactone + H2O
N-3-oxo-octanoyl-L-homoserine
-
-
-
?
N-3-oxo-octanoyl-L-homoserine lactone + H2O
N-3-oxo-octanoyl-L-homoserine
the substrate is derived from Agrobacterium tumefaciens KYC6
-
-
?
N-3-oxodecanoyl-L-homoserine lactone + H2O
N-3-oxodecanoyl-L-homoserine
-
-
-
?
N-3-oxodecanoyl-L-homoserine lactone + H2O
N-3-oxodecanoyl-L-homoserine
-
high activity
-
-
?
N-3-oxodecanoyl-L-homoserine lactone + H2O
N-3-oxodecanoyl-L-homoserine
-
-
-
?
N-3-oxodecanoyl-L-homoserine lactone + H2O
N-3-oxodecanoyl-L-homoserine
-
high activity
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
the substrate regulates production of virulence determinants of the human pathogen Pseudomonas aeruginosa
product identification by mass spectrometry
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
the substrate regulates production of virulence determinants of the human pathogen Pseudomonas aeruginosa
product identification by mass spectrometry
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
best substrate
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
best substrate
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
about 60% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
about 60% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
product identification by mass spectrometry
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
product identification by mass spectrometry
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
Q7B8C0, Q7B8C1, Q7B8C2, Q7B8C3, Q7B8C4, Q7B8C5, Q8KI79, Q8KTW4, Q8KTW5, Q8KTW6, Q8KTW7, Q8KTW8, Q8KTW9, Q8KTX0, Q8KTX1 -
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
best substrate
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
best substrate
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
?
N-3-oxohexanoyl-L-homoserine lactone + H2O
N-3-oxohexanoyl-L-homoserine
-
-
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
-
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
-
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
high activity
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
the substrate controls Ti plasmid conjugal transfer in Agrobacterium tumefaciens
product identification by mass spectrometry
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
the substrate controls Ti plasmid conjugal transfer in Agrobacterium tumefaciens
product identification by mass spectrometry
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
-
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
-
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
about 80% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
about 80% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
-
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
the isozymes AttM, AiiB, and AiiC inactivate the acylhomoserine lactone quorum-sensing signaling and attenuate the virulence of Erwinia carotovora subsp. atroseptica strain 6276 on Solanum tuberosum, function and regulation of quorum-sensing signaling, overview
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
the isozymes AttM, AiiB, and AiiC inactivate the acylhomoserine lactone quorum-sensing signaling and attenuate the virulence of Erwinia carotovora subsp. atroseptica strain 6276 on Solanum tuberosum, function and regulation of quorum-sensing signaling, overview
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
the acylhomoserine lactones are inducers of quorum-sensing signaling
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
the acylhomoserine lactones are inducers of quorum-sensing signaling
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
-
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
N-acylhomoserine lactones, AHL, regulate protease production, swarming motility, biofilm formation, and Caenorhabditis elegans killing efficiency in most strains of the Burkholderia complex organisms, which is inhibited by Bacillus AHL lactonase acting as a quorum-quenching enzyme
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain IBN98 on Solanum tuberosum, acylhomoserine lactones are autoinducers of quorum-sensing signaling
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, the inhibition of which is a feasible approach for prevention of bacterial infection
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the acylhomoserine lactones are inducers of quorum-sensing signaling
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
-
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
N-acylhomoserine lactones, AHL, regulate protease production, swarming motility, biofilm formation, and Caenorhabditis elegans killing efficiency in most strains of the Burkholderia complex organisms, which is inhibited by Bacillus AHL lactonase acting as a quorum-quenching enzyme
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, the inhibition of which is a feasible approach for prevention of bacterial infection
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain IBN98 on Solanum tuberosum, acylhomoserine lactones are autoinducers of quorum-sensing signaling
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
Q7B8C0, Q7B8C1, Q7B8C2, Q7B8C3, Q7B8C4, Q7B8C5, Q8KI79, Q8KTW4, Q8KTW5, Q8KTW6, Q8KTW7, Q8KTW8, Q8KTW9, Q8KTX0, Q8KTX1 the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain IBN98 on Solanum tuberosum, acylhomoserine lactones are autoinducers of quorum-sensing signaling
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the acylhomoserine lactones are inducers of quorum-sensing signaling
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain IBN98 on Solanum tuberosum, acylhomoserine lactones are autoinducers of quorum-sensing signaling
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
-
-
?
N-butanoyl-DL-homoserine lactone + H2O
N-butanoyl-DL-homoserine
-
relative activity: 190%
-
-
?
N-butanoyl-DL-homoserine lactone + H2O
N-butanoyl-DL-homoserine
-
-
-
-
?
N-butanoyl-L-homoserine lactone + H2O
N-butanoyl-L-homoserine
-
-
-
?
N-butanoyl-L-homoserine lactone + H2O
N-butanoyl-L-homoserine
-
-
-
?
N-butanoyl-L-homoserine lactone + H2O
N-butanoyl-L-homoserine
-
-
-
-
?
N-butanoyl-L-homoserine lactone + H2O
N-butanoyl-L-homoserine
-
high activity
-
-
?
N-butanoyl-L-homoserine lactone + H2O
N-butanoyl-L-homoserine
the substrate regulates production of virulence determinants of the human pathogen Pseudomonas aeruginosa
product identification by mass spectrometry
-
?
N-butanoyl-L-homoserine lactone + H2O
N-butanoyl-L-homoserine
-
-
-
-
?
N-butanoyl-L-homoserine lactone + H2O
N-butanoyl-L-homoserine
-
weak substrate
-
-
?
N-butyryl-DL-homoserine lactone + H2O
N-butyryl-DL-homoserine
-
-
-
-
?
N-butyryl-DL-homoserine lactone + H2O
N-butyryl-DL-homoserine
-
-
-
-
?
N-butyryl-DL-homoserine lactone + H2O
N-butyryl-DL-homoserine
-
-
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
-
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
-
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
the kcat/Km of wild-type enzyme for N-decanoyl-L-homoserine lactone is about 50fold higher than that for N-butyryl-L-homoserine lactone. The triple mutant (F64W/V69W/A206F) shows a 100fold shifted preference for N-butyryl-L-homoserine lactone
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
the kcat/Km of wild-type enzyme for N-decanoyl-L-homoserine lactone is about 50fold higher than that for N-butyryl-L-homoserine lactone. The triple mutant (F64W/V69W/A206F) shows a 100fold shifted preference for N-butyryl-L-homoserine lactone
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
-
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
-
-
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
-
substrate of mutant E101N/R230I, not of the wild-type enzyme
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
-
substrate for mutant enzymes E101G/R230C and E101N/R230I
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
-
-
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
-
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
-
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
-
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
-
about 10% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
-
-
-
-
?
N-butyryl-L-homoserine lactone + H2O
N-butyryl-L-homoserine
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
L-homoserine lactone + decanoate
best substrate
-
-
?
N-decanoyl-L-homoserine lactone + H2O
L-homoserine lactone + decanoate
best substrate
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
highest activity
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
high activity
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
the kcat/Km of wild-type enzyme for N-decanoyl-L-homoserine lactone is about 50fold higher than that for N-butyryl-L-homoserine lactone. The triple mutant (F64W/V69W/A206F) shows a 100fold shifted preference for N-butyryl-L-homoserine lactone
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
the kcat/Km of wild-type enzyme for N-decanoyl-L-homoserine lactone is about 50fold higher than that for N-butyryl-L-homoserine lactone. The triple mutant (F64W/V69W/A206F) shows a 100fold shifted preference for N-butyryl-L-homoserine lactone
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
best substrate
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
best substrate
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
relative activity: 95.6%
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
high activity
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
about 40% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
about 40% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
L-homoserine lactone + dodecanoate
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
L-homoserine lactone + dodecanoate
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
-
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
-
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
-
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
-
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
-
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
relative activity: 89.7%
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
-
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
-
-
-
-
?
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
-
-
-
?
N-heptanoyl-L-homoserine lactone + H2O
N-heptanoyl-L-homoserine
-
-
-
?
N-heptanoyl-L-homoserine lactone + H2O
N-heptanoyl-L-homoserine
-
-
-
-
?
N-heptanoyl-L-homoserine lactone + H2O
N-heptanoyl-L-homoserine
-
-
-
?
N-heptanoyl-L-homoserine lactone + H2O
N-heptanoyl-L-homoserine
-
about 10% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone
-
-
?
N-hexanoyl-(S)-homoserine lactone
N-hexanoyl-(S)-homoserine
-
-
-
?
N-hexanoyl-(S)-homoserine lactone
N-hexanoyl-(S)-homoserine
-
-
-
?
N-hexanoyl-(S)-homoserine lactone
N-hexanoyl-(S)-homoserine
-
-
-
?
N-hexanoyl-(S)-homoserine lactone + H2O
N-hexanoyl-(S)-homoserine
no activity with N-hexanoyl-(R)-homoserine lactone
-
-
?
N-hexanoyl-(S)-homoserine lactone + H2O
N-hexanoyl-(S)-homoserine
no activity with N-hexanoyl-(R)-homoserine lactone
-
-
?
N-hexanoyl-(S)-homoserine lactone + H2O
N-hexanoyl-(S)-homoserine
no activity with N-hexanoyl-(R)-homoserine lactone
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
L-homoserine lactone + hexanoate
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
L-homoserine lactone + hexanoate
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
product analysis of recombinant enzyme expressed in Escherichia coli strain JM109
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
product analysis of recombinant enzyme expressed in Escherichia coli strain JM109
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
best substrate
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
the structures of the lactonase bound with two different concentrations of the ring-opened product of N-hexanoyl-L-homoserine lactone exhibit different product configurations, overview
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
relative activity: 99.6%
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
lower activity
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
lower activity
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
about 90% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
about 90% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
product identification by mass spectrometry
-
-
r
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
product identification by mass spectrometry
-
-
r
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
product identification by mass spectrometry
-
-
r
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
product identification by mass spectrometry
-
-
r
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
product identification by mass spectrometry
-
-
r
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexyl-L-homoserine lactone + H2O
N-hexyl-L-homoserine
-
-
-
-
?
N-hexyl-L-homoserine lactone + H2O
N-hexyl-L-homoserine
-
-
-
-
?
N-hexyl-L-homoserine lactone + H2O
N-hexyl-L-homoserine
-
-
-
-
?
N-octanoyl-DL-homoserine lactone + H2O
N-octanoyl-DL-homoserine
-
relative activity: 457%
-
-
?
N-octanoyl-DL-homoserine lactone + H2O
N-octanoyl-DL-homoserine
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
L-homoserine lactone + octanoate
worst substrate
-
-
?
N-octanoyl-L-homoserine lactone + H2O
L-homoserine lactone + octanoate
worst substrate
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
high activity
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
relative activity: 98.9%
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
lower activity
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
lower activity
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
relative activity: 98.9%
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
about 55% of the activity compared to N-3-oxohexanoyl-L-homoserine lactone
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
?
N-pentanoyl-L-homoserine lactone + H2O
N-pentanoyl-L-homoserine
-
-
-
?
N-pentanoyl-L-homoserine lactone + H2O
N-pentanoyl-L-homoserine
-
-
-
?
N-tetradecanoyl-L-homoserine lactone + H2O
N-tetradecanoyl-L-homoserine
-
-
-
?
N-tetradecanoyl-L-homoserine lactone + H2O
N-tetradecanoyl-L-homoserine
-
-
-
-
?
N-tetradecanoyl-L-homoserine lactone + H2O
N-tetradecanoyl-L-homoserine
-
-
-
-
?
N-tetradecanoyl-L-homoserine lactone + H2O
N-tetradecanoyl-L-homoserine
-
-
-
-
?
N-tetradecanoyl-L-homoserine lactone + H2O
N-tetradecanoyl-L-homoserine
-
-
-
-
?
additional information
?
-
-
BlcC does not degrade the acyl-L-homoserine lactone bound by TraR in the quorum quenching system, overview. BlcC does not significantly affect transfer of the Ti plasmid in planta
-
-
?
additional information
?
-
-
degradation of acyl homoserine lactone signal molecules
-
-
?
additional information
?
-
-
BlcC does not degrade the acyl-L-homoserine lactone bound by TraR
-
-
?
additional information
?
-
substrate specificity, overview
-
-
?
additional information
?
-
quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview
-
-
?
additional information
?
-
-
quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview
-
-
?
additional information
?
-
quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview
-
-
?
additional information
?
-
-
N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence
-
-
?
additional information
?
-
-
substrate specificity, poor activity with non-acyl lactones and no activity with non-cyclic esters, but strong activity with all N-acyl-(S)-homoserine lactones of different chain length and nature, the amide group and the ketone at C1 position of the substrate's acyl chain might be important for substrate-enzyme interaction, overview
-
-
?
additional information
?
-
the substrate N-acyl-L-homoserine lactones show negligible enzyme-independent delactonization
-
-
?
additional information
?
-
-
the substrate N-acyl-L-homoserine lactones show negligible enzyme-independent delactonization
-
-
?
additional information
?
-
the substrate N-acyl-L-homoserine lactones show negligible enzyme-independent delactonization
-
-
?
additional information
?
-
the quorum sensing system of Aeromonas veronii LP-11 is quenched by AHL lactonase AiiAAI96, probably by enzymatically inactivating the N-acyl-homoserine lactones
-
-
?
additional information
?
-
substrate binding analysis by quantum mechanics/molecular mechanics (QM/MM) molecular dynamics simulations, overview
-
-
?
additional information
?
-
the enzyme possesses a broad substrate-binding cavity, structure, overview
-
-
?
additional information
?
-
-
the enzyme possesses a broad substrate-binding cavity, structure, overview
-
-
?
additional information
?
-
N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence
-
-
?
additional information
?
-
N-acyl homoserine lactone, AHL, quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence and biofilm formation of Gram-negative bacteria, the enzyme deactivates the signaling by degradation of AHLs via lactone ring hydrolysis
-
-
?
additional information
?
-
N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence
-
-
?
additional information
?
-
N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
enzyme additionally displays organophosphate hydrolase activity, EC 3.1.8.1
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
degradation of acyl homoserine lactone signal molecules
-
-
?
additional information
?
-
-
degradation of acyl homoserine lactone signal molecules
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
the wild-type enzyme does also show low hydrolysis activity with paraoxon, while its mutants do not
-
-
?
additional information
?
-
-
no activity towards N-butyryl-L-homoserine lactone, N-decanoyl-L-homoserine lactone, and N-(3-oxohexanoyl)-L-homoserine lactone
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
the h-PON1 can hydrolyze (and inactivate) a variety of substrates including aryl esters, thioesters, phosphotriesters, carbonates, lactones and thiolactones, and its various hydrolytic activities can be broadly grouped into three categories, namely arylesterase, organophosphatase, and lactonase. The native activity of h-PON1 seems to be lactonase. Polymorphism at position 192 plays an important role in determining the substrate specificity and catalytic efficiency of the enzyme
-
-
?
additional information
?
-
h-PON1 enzyme is also active with diethyl-paraoxon, diisopropyl fluorophosphate and chlorpyrifos-oxon, cf. EC 3.1.8.1 and EC 3.1.8.2
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview
-
-
?
additional information
?
-
-
quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview
-
-
?
additional information
?
-
quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview
-
-
?
additional information
?
-
poor activity with N-butyryl-L-homoserine lactone
-
-
?
additional information
?
-
-
poor activity with N-butyryl-L-homoserine lactone
-
-
?
additional information
?
-
poor activity with N-butyryl-L-homoserine lactone
-
-
?
additional information
?
-
-
poor activity with N-butyryl-L-homoserine lactone
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lacton ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
substrate specificity, N-butanoyl-L-homoserine lactone is a poor substrate
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lacton ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
substrate specificity, N-butanoyl-L-homoserine lactone is a poor substrate
-
-
?
additional information
?
-
-
substrate specificity, overview
-
-
?
additional information
?
-
-
the AHL-degrading enzyme catalyzes the lactone ring opening of N-3-oxohexanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone by hydrolyzing the lactones
-
-
?
additional information
?
-
-
substrate specificity, overview
-
-
?
additional information
?
-
-
the AHL-degrading enzyme catalyzes the lactone ring opening of N-3-oxohexanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone by hydrolyzing the lactones
-
-
?
additional information
?
-
-
the enzyme is involved in inactivation of N-acylhomoserine lactone signaling in regulation of infection and virulence functions
-
-
?
additional information
?
-
-
degradation of acyl homoserine lactone signal molecules
-
-
?
additional information
?
-
QsdA inactivates N-acyl homoserine lactones with an acyl chain ranging from C6 to C14, with or without substitution at carbon 3. N-AHSL lactonase is unrelated to N-AHSL-degrading enzymes, i.e., the lactonase AiiA and the amidohydrolase AiiD, overview. Bacteria harboring the qsdA gene interfere very efficiently with quorum-sensing-regulated functions, overview
-
-
?
additional information
?
-
-
QsdA inactivates N-acyl homoserine lactones with an acyl chain ranging from C6 to C14, with or without substitution at carbon 3. N-AHSL lactonase is unrelated to N-AHSL-degrading enzymes, i.e., the lactonase AiiA and the amidohydrolase AiiD, overview. Bacteria harboring the qsdA gene interfere very efficiently with quorum-sensing-regulated functions, overview
-
-
?
additional information
?
-
-
enzyme additionally displays organophosphate hydrolase activity, EC 3.1.8.1
-
-
?
additional information
?
-
-
degradation of acyl homoserine lactone signal molecules
-
-
?
additional information
?
-
-
enzyme additionally displays organophosphate hydrolase activity, EC 3.1.8.1
-
-
?
additional information
?
-
the phosphotriesterase-like lactonase enzyme is bifunctional showing lactonase (EC 3.1.1.81) and phosphotriesterase (EC 3.1.8.1) activities
-
-
?
additional information
?
-
no activity with coumaphos, fensulfothion, fenitrothion, diazinon, chlorpyrifos, and ethyl parathion. Low activity with malathion, methyl parathion methyl paraoxon, and ethyl paraoxon
-
-
?
additional information
?
-
the enzyme has activity on acyl-homoserine lactones and 5-thiobutyl butyrolactone (TBBL). Substrate docking analysis. The C258 residue in the active site is involved in an interaction with the oxygen atom from the amide in the lactone analogue. C258 residue might have a key role in the lactone hydrolysis mechanism
-
-
?
additional information
?
-
-
the enzyme has lactonase activity with a preference toward N-acyl homoserine lactones with 8-10 carbon aliphatic chains and oxo-lactones with shorter chains
-
-
?
additional information
?
-
the enzyme is also active on diethyl-paraoxon, dimethyl-paraoxon, diethyl-parathion, dimethyl-parathion, and malathion. The lactone ring hydrolysis is followed by acidification of the medium for which Cresol purple serves as pH indicator. No or poor activity is detected against phenylacetate, dihydrocoumarin, 4-nitrophenyl acetate, 4-nitrophenyl decanoate, and AHLs with short aliphatic substituents (4 to 6 carbon atoms), e.g. N-hexanoyl-DL-homoserine lactone, N-butanoyl-DL-homoserine lactone, and N-3-oxo-hexanoyl-DL-homoserine lactone. AHLs with longer substituents are better substrates for VmoLac, including C8 and C10-AHLs. Oxo-lactones are the best substrates for VmoLac. gamma-caprolactone and gamma-heptalactone are degraded with approximately the same catalytic efficiencies, and oxo-lactones with long aliphatic chains such as nonanoic-gamma-lactone and undecanoic-delta-lactone, are hydrolyzed with high catalytic efficiencies
-
-
?
additional information
?
-
-
the enzyme is also active on diethyl-paraoxon, dimethyl-paraoxon, diethyl-parathion, dimethyl-parathion, and malathion. The lactone ring hydrolysis is followed by acidification of the medium for which Cresol purple serves as pH indicator. No or poor activity is detected against phenylacetate, dihydrocoumarin, 4-nitrophenyl acetate, 4-nitrophenyl decanoate, and AHLs with short aliphatic substituents (4 to 6 carbon atoms), e.g. N-hexanoyl-DL-homoserine lactone, N-butanoyl-DL-homoserine lactone, and N-3-oxo-hexanoyl-DL-homoserine lactone. AHLs with longer substituents are better substrates for VmoLac, including C8 and C10-AHLs. Oxo-lactones are the best substrates for VmoLac. gamma-caprolactone and gamma-heptalactone are degraded with approximately the same catalytic efficiencies, and oxo-lactones with long aliphatic chains such as nonanoic-gamma-lactone and undecanoic-delta-lactone, are hydrolyzed with high catalytic efficiencies
-
-
?
additional information
?
-
VmoLac is a proficient lactonase with promiscuous, poor esterase and phosphotriesterase activities
-
-
?
additional information
?
-
-
VmoLac is a proficient lactonase with promiscuous, poor esterase and phosphotriesterase activities
-
-
?
additional information
?
-
VmoLac is a proficient lactonase with promiscuous, poor esterase and phosphotriesterase activities
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
homo-L-cysteine thiolactone + H2O
homo-L-cysteine
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
N-(3-oxooctanoyl)-L-homoserine lactone + H2O
N-(3-oxooctanoyl)-L-homoserine
-
-
-
-
?
N-3-oxo-dodecanoyl-L-homoserine lactone + H2O
N-3-oxo-dodecanoyl-L-homoserine
-
-
-
-
?
N-3-oxo-hexanoyl-L-homoserine lactone + H2O
N-3-oxo-hexanoyl-L-homoserine
-
lower activity
-
-
?
N-3-oxo-octanoyl-L-homoserine lactone + H2O
N-3-oxo-octanoyl-L-homoserine
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
N-dodecanoyl-L-homoserine lactone + H2O
N-dodecanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
additional information
?
-
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
-
-
-
?
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
-
-
-
?
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
-
-
-
-
?
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
-
-
-
?
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
Tritonibacter mobilis
-
-
-
-
?
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
Tritonibacter mobilis YJ3
-
-
-
-
?
an N-acyl-L-homoserine lactone + H2O
an N-acyl-L-homoserine
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
-
-
-
-
?
N-(3-oxohexanoyl)-L-homoserine lactone + H2O
N-(3-oxohexanoyl)-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxododecanoyl-L-homoserine lactone + H2O
N-3-oxododecanoyl-L-homoserine
-
-
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
-
-
-
?
N-3-oxooctanoyl-L-homoserine lactone + H2O
N-3-oxooctanoyl-L-homoserine
-
-
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
the isozymes AttM, AiiB, and AiiC inactivate the acylhomoserine lactone quorum-sensing signaling and attenuate the virulence of Erwinia carotovora subsp. atroseptica strain 6276 on Solanum tuberosum, function and regulation of quorum-sensing signaling, overview
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
the isozymes AttM, AiiB, and AiiC inactivate the acylhomoserine lactone quorum-sensing signaling and attenuate the virulence of Erwinia carotovora subsp. atroseptica strain 6276 on Solanum tuberosum, function and regulation of quorum-sensing signaling, overview
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
N-acylhomoserine lactones, AHL, regulate protease production, swarming motility, biofilm formation, and Caenorhabditis elegans killing efficiency in most strains of the Burkholderia complex organisms, which is inhibited by Bacillus AHL lactonase acting as a quorum-quenching enzyme
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain IBN98 on Solanum tuberosum, acylhomoserine lactones are autoinducers of quorum-sensing signaling
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, the inhibition of which is a feasible approach for prevention of bacterial infection
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
-
N-acylhomoserine lactones, AHL, regulate protease production, swarming motility, biofilm formation, and Caenorhabditis elegans killing efficiency in most strains of the Burkholderia complex organisms, which is inhibited by Bacillus AHL lactonase acting as a quorum-quenching enzyme
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, the inhibition of which is a feasible approach for prevention of bacterial infection
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, mechanism
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain IBN98 on Solanum tuberosum, acylhomoserine lactones are autoinducers of quorum-sensing signaling
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence bacteria on plants
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
Q7B8C0, Q7B8C1, Q7B8C2, Q7B8C3, Q7B8C4, Q7B8C5, Q8KI79, Q8KTW4, Q8KTW5, Q8KTW6, Q8KTW7, Q8KTW8, Q8KTW9, Q8KTX0, Q8KTX1 the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain IBN98 on Solanum tuberosum, acylhomoserine lactones are autoinducers of quorum-sensing signaling
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain IBN98 on Solanum tuberosum, acylhomoserine lactones are autoinducers of quorum-sensing signaling
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria
-
-
?
N-acyl-(S)-homoserine lactone + H2O
N-acyl-(S)-homoserine
the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-decanoyl-L-homoserine lactone + H2O
N-decanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
lower activity
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
lower activity
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
-
?
N-hexanoyl-L-homoserine lactone + H2O
N-hexanoyl-L-homoserine
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
lower activity
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
lower activity
-
-
?
N-octanoyl-L-homoserine lactone + H2O
N-octanoyl-L-homoserine
-
-
-
?
additional information
?
-
-
BlcC does not degrade the acyl-L-homoserine lactone bound by TraR in the quorum quenching system, overview. BlcC does not significantly affect transfer of the Ti plasmid in planta
-
-
?
additional information
?
-
-
degradation of acyl homoserine lactone signal molecules
-
-
?
additional information
?
-
quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview
-
-
?
additional information
?
-
-
quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview
-
-
?
additional information
?
-
quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview
-
-
?
additional information
?
-
-
N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence
-
-
?
additional information
?
-
N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence
-
-
?
additional information
?
-
N-acyl homoserine lactone, AHL, quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence and biofilm formation of Gram-negative bacteria, the enzyme deactivates the signaling by degradation of AHLs via lactone ring hydrolysis
-
-
?
additional information
?
-
N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence
-
-
?
additional information
?
-
N-acyl homoserine lactone quorum-sensing signals are the vital elements of bacterial quorum-sensing systems, which regulate diverse biological functions, including virulence
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
degradation of acyl homoserine lactone signal molecules
-
-
?
additional information
?
-
-
degradation of acyl homoserine lactone signal molecules
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
the h-PON1 can hydrolyze (and inactivate) a variety of substrates including aryl esters, thioesters, phosphotriesters, carbonates, lactones and thiolactones, and its various hydrolytic activities can be broadly grouped into three categories, namely arylesterase, organophosphatase, and lactonase. The native activity of h-PON1 seems to be lactonase. Polymorphism at position 192 plays an important role in determining the substrate specificity and catalytic efficiency of the enzyme
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview
-
-
?
additional information
?
-
-
quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview
-
-
?
additional information
?
-
quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lactone ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lacton ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
acyl-homoserine lactone quorum-sensing signals play a key role in synchronizing virulence gene expression during bloodstream infections of mammals, the enzyme inactivates the AHL signaling by hydrolysis of the lacton ring thus acting as quorum-quenching enzyme
-
-
?
additional information
?
-
-
the AHL-degrading enzyme catalyzes the lactone ring opening of N-3-oxohexanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone by hydrolyzing the lactones
-
-
?
additional information
?
-
-
the AHL-degrading enzyme catalyzes the lactone ring opening of N-3-oxohexanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone by hydrolyzing the lactones
-
-
?
additional information
?
-
-
the enzyme is involved in inactivation of N-acylhomoserine lactone signaling in regulation of infection and virulence functions
-
-
?
additional information
?
-
-
degradation of acyl homoserine lactone signal molecules
-
-
?
additional information
?
-
QsdA inactivates N-acyl homoserine lactones with an acyl chain ranging from C6 to C14, with or without substitution at carbon 3. N-AHSL lactonase is unrelated to N-AHSL-degrading enzymes, i.e., the lactonase AiiA and the amidohydrolase AiiD, overview. Bacteria harboring the qsdA gene interfere very efficiently with quorum-sensing-regulated functions, overview
-
-
?
additional information
?
-
-
QsdA inactivates N-acyl homoserine lactones with an acyl chain ranging from C6 to C14, with or without substitution at carbon 3. N-AHSL lactonase is unrelated to N-AHSL-degrading enzymes, i.e., the lactonase AiiA and the amidohydrolase AiiD, overview. Bacteria harboring the qsdA gene interfere very efficiently with quorum-sensing-regulated functions, overview
-
-
?
additional information
?
-
-
degradation of acyl homoserine lactone signal molecules
-
-
?
additional information
?
-
the phosphotriesterase-like lactonase enzyme is bifunctional showing lactonase (EC 3.1.1.81) and phosphotriesterase (EC 3.1.8.1) activities
-
-
?
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0.55
(R)-gamma-caprolactone
pH 8.0, 25°C, recombinant enzyme
0.75
(S)-gamma-caprolactone
pH 8.0, 25°C, recombinant enzyme
0.0174
3-oxo-dodecanoyl-L-homoserine lactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
0.0185 - 18.5
delta-decalactone
0.839
delta-dodecalactone
pH and temperature not specified in the publication
0.035 - 1.22
delta-nonalactone
0.105
delta-undecalactone
pH and temperature not specified in the publication
0.0187 - 18.7
delta-valerolactone
0.839
dodecanoic-delta-lactone
pH 8.0, 25°C, recombinant enzyme
0.0021
dodecanoic-gamma-lactone
pH 8.0, 25°C, recombinant enzyme
0.171
gamma-butyrolactone
3.709
Gamma-caprolactone
pH and temperature not specified in the publication
0.0021
gamma-dodecalactone
pH and temperature not specified in the publication
0.872
gamma-heptalactone
pH and temperature not specified in the publication
0.0369 - 36.9
gamma-heptanolide
0.0024 - 1.1
gamma-nonalactone
0.12
gamma-undecalactone
pH and temperature not specified in the publication
0.872
heptanoic-gamma-lactone
pH 8.0, 25°C, recombinant enzyme
0.306
L-homoserine lactone
-
pH 8.2, 25°C
0.224
N-(3-hydroxyoctanoyl)-DL-homoserine lactone
-
pH 8.0, 22°C, recombinant enzyme
0.05
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.0, 25°C
0.184
N-(3-oxodecyl)-L-homoserine lactone
-
pH 8.2, 25°C
0.101 - 0.74
N-(3-oxododecanoyl)-L-homoserine lactone
0.101
N-(3-oxododecyl)-L-homoserine lactone
-
pH 8.2, 25°C
2.9 - 26
N-(3-oxohexanoyl)-L-homoserine lactone
0.16 - 9
N-(3-oxooctanoyl)-L-homoserine lactone
0.608
N-(3-oxooctyl)-L-homoserine lactone
-
pH 8.2, 25°C
0.0075
N-3-hydroxybutanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
0.231
N-3-oxo-decanoyl-DL-homoserine lactone
pH 8.0, 25°C, recombinant enzyme
0.48 - 0.74
N-3-oxo-dodecanoyl-L-homoserine lactone
9 - 26
N-3-oxo-hexanoyl-L-homoserine lactone
0.186
N-3-oxo-octanoyl-DL-homoserine lactone
pH 8.0, 25°C, recombinant enzyme
2.9 - 5.9
N-3-oxo-octanoyl-L-homoserine lactone
0.0047
N-3-oxobutanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
0.0014
N-3-oxodecanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
0.00219 - 15.21
N-3-oxododecanoyl-L-homoserine lactone
0.003 - 0.95
N-3-oxohexanoyl-L-homoserine lactone
0.0023 - 0.49
N-3-oxooctanoyl-L-homoserine lactone
0.413 - 0.5
N-butanoyl-DL-homoserine lactone
0.0051 - 6.01
N-butanoyl-L-homoserine lactone
0.412
N-butyryl-DL-homoserine lactone
-
pH 8.2, 25°C
0.0094 - 39.5
N-butyryl-L-homoserine lactone
0.00145 - 14.88
N-decanoyl-L-homoserine lactone
0.007
N-dodecanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
8.84
N-heptanoyl-DL-homoserine lactone
-
pH 7.4, 32°C
0.047 - 1.6
N-heptanoyl-L-homoserine lactone
6.7
N-hexanoyl-(S)-homoserine lactone
pH 7.4, 28°C, purified detagged recombinant enzyme
0.86
N-hexanoyl-DL-homoserine lactone
-
pH 7.4, 32°C
0.003089 - 10
N-hexanoyl-L-homoserine lactone
0.6 - 1.3
N-hexanoyl-L-homoserine thiolactone
0.526
N-hexyl-L-homoserine lactone
-
pH 8.2, 25°C
0.262 - 3.81
N-octanoyl-DL-homoserine lactone
0.0026 - 10.35
N-octanoyl-L-homoserine lactone
0.13 - 6
N-pentanoyl-L-homoserine lactone
0.036
N-tetradecanoyl-L-homoserine lactone
-
pH 7.4, 25°C
1.22
nonanoic-delta-lactone
pH 8.0, 25°C, recombinant enzyme
0.047
nonanoic-gamma-lactone
pH 8.0, 25°C, recombinant enzyme
0.69
paraoxon
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
3.709
rac-gamma-caprolactone
pH 8.0, 25°C, recombinant enzyme
0.105
undecanoic-delta-lactone
pH 8.0, 25°C, recombinant enzyme
0.12
undecanoic-gamma-lactone
pH 8.0, 25°C, recombinant enzyme
additional information
additional information
-
0.0185
delta-decalactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
18.5
delta-decalactone
pH 8.3, 25°C
0.035
delta-nonalactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
0.035
delta-nonalactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
1.1
delta-nonalactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
1.1
delta-nonalactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
1.22
delta-nonalactone
pH and temperature not specified in the publication
0.0187
delta-valerolactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
18.7
delta-valerolactone
pH 8.3, 25°C
0.171
gamma-butyrolactone
pH 8.3, 25°C
0.171
gamma-butyrolactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
0.0369
gamma-heptanolide
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
36.9
gamma-heptanolide
pH 8.3, 25°C
0.0024
gamma-nonalactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
0.024
gamma-nonalactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.047
gamma-nonalactone
pH and temperature not specified in the publication
0.46
gamma-nonalactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
0.46
gamma-nonalactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
1.1
gamma-nonalactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.101
N-(3-oxododecanoyl)-L-homoserine lactone
-
pH 8.0, 22°C, recombinant enzyme
0.17
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.0, 25°C
0.48
N-(3-oxododecanoyl)-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.74
N-(3-oxododecanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
2.9
N-(3-oxohexanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
5.6
N-(3-oxohexanoyl)-L-homoserine lactone
pH 8.0, 25°C
9
N-(3-oxohexanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
26
N-(3-oxohexanoyl)-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.16
N-(3-oxooctanoyl)-L-homoserine lactone
pH 8.0, 25°C
2.9
N-(3-oxooctanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
3.8
N-(3-oxooctanoyl)-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
5.9
N-(3-oxooctanoyl)-L-homoserine lactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
9
N-(3-oxooctanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.48
N-3-oxo-dodecanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
0.74
N-3-oxo-dodecanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
9
N-3-oxo-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
26
N-3-oxo-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
2.9
N-3-oxo-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
3.8
N-3-oxo-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
5.9
N-3-oxo-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
0.00219
N-3-oxododecanoyl-L-homoserine lactone
pH 8.3, 25°C
0.014
N-3-oxododecanoyl-L-homoserine lactone
pH 8.3, 25°C
0.0174
N-3-oxododecanoyl-L-homoserine lactone
pH 8.3, 25°C
0.44
N-3-oxododecanoyl-L-homoserine lactone
pH 7.1, 25°C
15.21
N-3-oxododecanoyl-L-homoserine lactone
-
pH 7.2, 30°C
0.003
N-3-oxohexanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
0.066
N-3-oxohexanoyl-L-homoserine lactone
-
pH 7.4, 25°C
0.95
N-3-oxohexanoyl-L-homoserine lactone
pH 7.1, 25°C
0.0023
N-3-oxooctanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
0.13
N-3-oxooctanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
0.186
N-3-oxooctanoyl-L-homoserine lactone
pH and temperature not specified in the publication
0.49
N-3-oxooctanoyl-L-homoserine lactone
pH 7.1, 25°C
0.413
N-butanoyl-DL-homoserine lactone
-
pH 8.0, 22°C, recombinant enzyme
0.5
N-butanoyl-DL-homoserine lactone
-
pH 7.4, 32°C
0.0051
N-butanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
0.0105
N-butanoyl-L-homoserine lactone
pH 8.3, 25°C
6.01
N-butanoyl-L-homoserine lactone
-
pH 7.2, 30°C
0.0094
N-butyryl-L-homoserine lactone
pH 8.3, 25°C
0.0094
N-butyryl-L-homoserine lactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
0.229
N-butyryl-L-homoserine lactone
pH 8.3, 25°C
0.47
N-butyryl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
0.85
N-butyryl-L-homoserine lactone
pH 7.1, 25°C
1.3
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/V69W/A206F
2.8
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/A206F
4.9
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/V69W
5.42
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69F
6.1
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69W/A206F
6.42
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69W
7.1
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64A
8.7
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206W
8.88
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206V
11.7
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206L
12.6
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206F
13
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69I
13.4
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69L
14.3
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69A
16.1
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W
27.3
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206I
27.5
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, wild-type enzyme
39.5
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64L
0.00145
N-decanoyl-L-homoserine lactone
pH 8.3, 25°C
0.012
N-decanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
0.0147
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206V
0.0496
N-decanoyl-L-homoserine lactone
pH 8.3, 25°C
0.0572
N-decanoyl-L-homoserine lactone
pH 8.3, 25°C
0.0572
N-decanoyl-L-homoserine lactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
0.0614
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206L
0.094
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69L
0.111
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/V69W
0.127
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69W/A206F
0.13
N-decanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
0.13
N-decanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
0.13
N-decanoyl-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.13
N-decanoyl-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.14
N-decanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
0.14
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206W
0.15
N-decanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
0.169
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206F
0.195
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69W
0.206
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69F
0.208
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64A
0.218
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69I
0.224
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/A206F
0.23
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W
0.231
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication
0.315
N-decanoyl-L-homoserine lactone
-
pH 7.4, 25°C
0.315
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206I
0.358
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69A
0.385
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64L
0.419
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/V69W/A206F
0.424
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, wild-type enzyme
14.88
N-decanoyl-L-homoserine lactone
-
pH 7.2, 30°C
0.047
N-heptanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
0.3
N-heptanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
1.6
N-heptanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
0.003089
N-hexanoyl-L-homoserine lactone
pH 7.4, 30°C
0.0038
N-hexanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
0.00797
N-hexanoyl-L-homoserine lactone
pH 8.3, 25°C
0.061
N-hexanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
0.0827
N-hexanoyl-L-homoserine lactone
pH 8.3, 25°C
0.0827
N-hexanoyl-L-homoserine lactone
-
pH 8.3, 25°C
0.0827
N-hexanoyl-L-homoserine lactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
0.09
N-hexanoyl-L-homoserine lactone
mutant D108N with Co2+
0.18
N-hexanoyl-L-homoserine lactone
-
Mn2+-form of AHL lactonase, at pH 7.4 and at 28°C
0.204
N-hexanoyl-L-homoserine lactone
-
pH 7.4, 25°C
0.24
N-hexanoyl-L-homoserine lactone
-
Cd2+-form of AHL lactonase, at pH 7.4 and at 28°C
0.36
N-hexanoyl-L-homoserine lactone
-
Co2+-form of AHL lactonase, at pH 7.4 and at 28°C
0.79
N-hexanoyl-L-homoserine lactone
pH 7.1, 25°C
1.6
N-hexanoyl-L-homoserine lactone
mutant D108N with Zn2+
2
N-hexanoyl-L-homoserine lactone
mutant Y194F with Co2+
2.1
N-hexanoyl-L-homoserine lactone
mutant Y194F with Zn2+
2.1
N-hexanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
2.4
N-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
2.4
N-hexanoyl-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
2.6
N-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
2.6
N-hexanoyl-L-homoserine lactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
3.7
N-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
3.7
N-hexanoyl-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
5.6
N-hexanoyl-L-homoserine lactone
-
Zn2+-form of AHL lactonase, at pH 7.4 and at 28°C
7.88
N-hexanoyl-L-homoserine lactone
-
pH 7.2, 30°C
10
N-hexanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
0.6
N-hexanoyl-L-homoserine thiolactone
mutant Y194F
1.3
N-hexanoyl-L-homoserine thiolactone
mutant D108N
0.262
N-octanoyl-DL-homoserine lactone
pH 8.0, 25°C, recombinant enzyme
3.81
N-octanoyl-DL-homoserine lactone
-
pH 7.4, 32°C
0.0026
N-octanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
0.083
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
0.095
N-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
0.095
N-octanoyl-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.18
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
0.26
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F64C/F68C, presence of DTT
0.262
N-octanoyl-L-homoserine lactone
pH and temperature not specified in the publication
0.39
N-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
0.39
N-octanoyl-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.44
N-octanoyl-L-homoserine lactone
pH 7.1, 25°C
0.7
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F64C/F68C, absence of DTT
0.7
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type, presence of DTT
0.8
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
1.2
N-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
1.2
N-octanoyl-L-homoserine lactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
10.35
N-octanoyl-L-homoserine lactone
-
pH 7.2, 30°C
0.13
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
1.8
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F64C/F68C, presence of DTT
3
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
4.5
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F64C/F68C, absence of DTT
6
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
6
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type, presence of DTT
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics and thermodynamics
-
additional information
additional information
steady-state kinetics, different enzyme constitutions
-
additional information
additional information
steady-state kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
-
steady-state kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
-
antibiotics binding kinetics at pH 7.5 and pH 10.5, overview. Kinetic analysis of the AHL lactonase activity of His6-OPH and its enzyme-polyelectrolyte complex (EPC) in the presence of antibiotics
-
additional information
additional information
gamma- and delta-dodecanoic lactone substrates have an allosteric profile of hydrolysis
-
additional information
additional information
-
gamma- and delta-dodecanoic lactone substrates have an allosteric profile of hydrolysis
-
additional information
additional information
kinetic analysis of lactonase activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3.04
(R)-gamma-caprolactone
pH 8.0, 25°C, recombinant enzyme
1.89
(S)-gamma-caprolactone
pH 8.0, 25°C, recombinant enzyme
4.9
3-oxo-dodecanoyl-L-homoserine lactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
28.08
delta-dodecalactone
pH and temperature not specified in the publication
1.6 - 88.91
delta-nonalactone
60.78
delta-undecalactone
pH and temperature not specified in the publication
28.08
dodecanoic-delta-lactone
pH 8.0, 25°C, recombinant enzyme
0.16
dodecanoic-gamma-lactone
pH 8.0, 25°C, recombinant enzyme
112.3
Gamma-caprolactone
pH and temperature not specified in the publication
0.16
gamma-dodecalactone
pH and temperature not specified in the publication
27.25
gamma-heptalactone
pH and temperature not specified in the publication
2 - 44.49
gamma-nonalactone
8.86
gamma-undecalactone
pH and temperature not specified in the publication
27.25
heptanoic-gamma-lactone
pH 8.0, 25°C, recombinant enzyme
4.69
L-homoserine lactone
-
pH 8.2, 25°C
1.5
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.0, 25°C
2.67
N-(3-oxodecyl)-L-homoserine lactone
-
pH 8.2, 25°C
0.95 - 6.9
N-(3-oxododecanoyl)-L-homoserine lactone
1.83
N-(3-oxododecyl)-L-homoserine lactone
-
pH 8.2, 25°C
0.4 - 1.8
N-(3-oxohexanoyl)-L-homoserine lactone
0.29 - 1.8
N-(3-oxooctanoyl)-L-homoserine lactone
4.66
N-(3-oxooctyl)-L-homoserine lactone
-
pH 8.2, 25°C
29.3
N-3-hydroxybutanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
0.47
N-3-oxo-decanoyl-DL-homoserine lactone
pH 8.0, 25°C, recombinant enzyme
2 - 6.9
N-3-oxo-dodecanoyl-L-homoserine lactone
0.4 - 0.91
N-3-oxo-hexanoyl-L-homoserine lactone
0.35
N-3-oxo-octanoyl-DL-homoserine lactone
pH 8.0, 25°C, recombinant enzyme
0.29 - 1.8
N-3-oxo-octanoyl-L-homoserine lactone
28.6
N-3-oxobutanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
20.2
N-3-oxodecanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
4.9 - 224
N-3-oxododecanoyl-L-homoserine lactone
0.168 - 293
N-3-oxohexanoyl-L-homoserine lactone
0.35 - 218
N-3-oxooctanoyl-L-homoserine lactone
0.00549
N-butanoyl-DL-homoserine lactone
-
pH 7.4, 32°C
13.54 - 61.14
N-butanoyl-L-homoserine lactone
2.62
N-butyryl-DL-homoserine lactone
-
pH 8.2, 25°C
0.009 - 236
N-butyryl-L-homoserine lactone
0.32 - 98.31
N-decanoyl-L-homoserine lactone
0.4
N-dodecanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
0.0235
N-heptanoyl-DL-homoserine lactone
-
pH 7.4, 32°C
0.3 - 80
N-heptanoyl-L-homoserine lactone
117
N-hexanoyl-(S)-homoserine lactone
pH 7.4, 28°C, purified detagged recombinant enzyme
0.0102
N-hexanoyl-DL-homoserine lactone
-
pH 7.4, 32°C
0.17 - 510
N-hexanoyl-L-homoserine lactone
1.11 - 5.4
N-hexanoyl-L-homoserine thiolactone
3.17
N-hexyl-L-homoserine lactone
-
pH 8.2, 25°C
0.0446 - 0.59
N-octanoyl-DL-homoserine lactone
0.3 - 158
N-octanoyl-L-homoserine lactone
0.08 - 57
N-pentanoyl-L-homoserine lactone
0.038
N-tetradecanoyl-L-homoserine lactone
-
pH 7.4, 25°C
88.91
nonanoic-delta-lactone
pH 8.0, 25°C, recombinant enzyme
44.49
nonanoic-gamma-lactone
pH 8.0, 25°C, recombinant enzyme
0.0031
paraoxon
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
112.3
rac-gamma-caprolactone
pH 8.0, 25°C, recombinant enzyme
60.78
undecanoic-delta-lactone
pH 8.0, 25°C, recombinant enzyme
8.86
undecanoic-gamma-lactone
pH 8.0, 25°C, recombinant enzyme
8.4
delta-decalactone
pH 8.3, 25°C
8.4
delta-decalactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
1.6
delta-nonalactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
1.6
delta-nonalactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
29
delta-nonalactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
29
delta-nonalactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
88.91
delta-nonalactone
pH and temperature not specified in the publication
8.4
delta-valerolactone
pH 8.3, 25°C
8.4
delta-valerolactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
23.5
gamma-butyrolactone
pH 8.3, 25°C
23.5
gamma-butyrolactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
7
gamma-heptanolide
pH 8.3, 25°C
7
gamma-heptanolide
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
2
gamma-nonalactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
2
gamma-nonalactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
2.9
gamma-nonalactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
2.9
gamma-nonalactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
22
gamma-nonalactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
44.49
gamma-nonalactone
pH and temperature not specified in the publication
0.95
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.0, 25°C
2
N-(3-oxododecanoyl)-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
6.9
N-(3-oxododecanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.4
N-(3-oxohexanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.52
N-(3-oxohexanoyl)-L-homoserine lactone
pH 8.0, 25°C
0.91
N-(3-oxohexanoyl)-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
1.8
N-(3-oxohexanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.29
N-(3-oxooctanoyl)-L-homoserine lactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.4
N-(3-oxooctanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
1
N-(3-oxooctanoyl)-L-homoserine lactone
pH 8.0, 25°C
1.1
N-(3-oxooctanoyl)-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
1.8
N-(3-oxooctanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
2
N-3-oxo-dodecanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
6.9
N-3-oxo-dodecanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
0.4
N-3-oxo-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
0.91
N-3-oxo-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
0.29
N-3-oxo-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
1.1
N-3-oxo-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
1.8
N-3-oxo-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
4.9
N-3-oxododecanoyl-L-homoserine lactone
pH 8.3, 25°C
5.03
N-3-oxododecanoyl-L-homoserine lactone
pH 8.3, 25°C
9.48
N-3-oxododecanoyl-L-homoserine lactone
pH 8.3, 25°C
101.5
N-3-oxododecanoyl-L-homoserine lactone
-
pH 7.2, 30°C
224
N-3-oxododecanoyl-L-homoserine lactone
pH 7.1, 25°C
0.168
N-3-oxohexanoyl-L-homoserine lactone
-
pH 7.4, 25°C
22.7
N-3-oxohexanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
293
N-3-oxohexanoyl-L-homoserine lactone
pH 7.1, 25°C
0.35
N-3-oxooctanoyl-L-homoserine lactone
pH and temperature not specified in the publication
2 - 8
N-3-oxooctanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
22.2
N-3-oxooctanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
218
N-3-oxooctanoyl-L-homoserine lactone
pH 7.1, 25°C
13.54
N-butanoyl-L-homoserine lactone
pH 8.3, 25°C
37.6
N-butanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
61.14
N-butanoyl-L-homoserine lactone
-
pH 7.2, 30°C
0.009
N-butyryl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
0.009
N-butyryl-L-homoserine lactone
-
kcat below 0.009 s-1, mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.011
N-butyryl-L-homoserine lactone
-
kcat below 0.011 s-1, mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.57
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64A
1.07
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206V
3.06
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206I
12.6
N-butyryl-L-homoserine lactone
pH 8.3, 25°C
12.6
N-butyryl-L-homoserine lactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
14.6
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206L
19.06
N-butyryl-L-homoserine lactone
pH 8.3, 25°C
39
N-butyryl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
40.2
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69F
51.7
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206W
59.1
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64L
59.4
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/V69W/A206F
61.5
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/A206F
69.3
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69I
93.2
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69A
104
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206F
121
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, wild-type enzyme
135
N-butyryl-L-homoserine lactone
pH 7.1, 25°C
138
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69L
159
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W
174
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69W
192
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69W/A206F
236
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/V69W
0.32
N-decanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
0.43
N-decanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
0.43
N-decanoyl-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.47
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication
1.05
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206V
1.321
N-decanoyl-L-homoserine lactone
-
pH 7.4, 25°C
1.7
N-decanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
1.7
N-decanoyl-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
2.98
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64A
4.16
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206I
5.13
N-decanoyl-L-homoserine lactone
pH 8.3, 25°C
5.2
N-decanoyl-L-homoserine lactone
pH 8.3, 25°C
5.2
N-decanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
5.2
N-decanoyl-L-homoserine lactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
5.48
N-decanoyl-L-homoserine lactone
pH 8.3, 25°C
7.41
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W
7.83
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/V69W
8.63
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206L
8.72
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/V69W/A206F
12.5
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69L
16.4
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69W/A206F
19.9
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69F
21.1
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/A206F
25.9
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69W
27.3
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69I
29
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64L
38.5
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206W
42
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206F
54
N-decanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
75
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69A
89
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, wild-type enzyme
98.31
N-decanoyl-L-homoserine lactone
-
pH 7.2, 30°C
0.3
N-heptanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
34
N-heptanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
80
N-heptanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
0.17
N-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
0.17
N-hexanoyl-L-homoserine lactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.2
N-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
0.2
N-hexanoyl-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.25
N-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
0.25
N-hexanoyl-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.257
N-hexanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
0.31
N-hexanoyl-L-homoserine lactone
mutant Y194F with Zn2+
0.649
N-hexanoyl-L-homoserine lactone
-
pH 7.4, 25°C
1.4
N-hexanoyl-L-homoserine lactone
mutant D108N with Zn2+
3 - 6
N-hexanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
3.5
N-hexanoyl-L-homoserine lactone
mutant D108N with Co2+
8.95
N-hexanoyl-L-homoserine lactone
pH 8.3, 25°C
13.97
N-hexanoyl-L-homoserine lactone
pH 8.3, 25°C
14
N-hexanoyl-L-homoserine lactone
pH 8.3, 25°C
14
N-hexanoyl-L-homoserine lactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
16.6
N-hexanoyl-L-homoserine lactone
mutant Y194F with Co2+
35.7
N-hexanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
59
N-hexanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
69.43
N-hexanoyl-L-homoserine lactone
-
pH 7.2, 30°C
91
N-hexanoyl-L-homoserine lactone
-
Zn2+-form of AHL lactonase, at pH 7.4 and at 28°C
226
N-hexanoyl-L-homoserine lactone
pH 7.1, 25°C
330
N-hexanoyl-L-homoserine lactone
-
Mn2+-form of AHL lactonase, at pH 7.4 and at 28°C
480
N-hexanoyl-L-homoserine lactone
-
Cd2+-form of AHL lactonase, at pH 7.4 and at 28°C
510
N-hexanoyl-L-homoserine lactone
-
Co2+-form of AHL lactonase, at pH 7.4 and at 28°C
1.11
N-hexanoyl-L-homoserine thiolactone
mutant D108N
5.4
N-hexanoyl-L-homoserine thiolactone
mutant Y194F
0.0446
N-octanoyl-DL-homoserine lactone
-
pH 7.4, 32°C
0.59
N-octanoyl-DL-homoserine lactone
pH 8.0, 25°C, recombinant enzyme
0.3
N-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
0.3
N-octanoyl-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.46
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
0.52
N-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
0.52
N-octanoyl-L-homoserine lactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.59
N-octanoyl-L-homoserine lactone
pH and temperature not specified in the publication
0.83
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F64C/F68C, absence of DTT
1.4
N-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
1.4
N-octanoyl-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
8.2
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F64C/F68C, presence of DTT
27.5
N-octanoyl-L-homoserine lactone
-
pH 7.4, 22°C, recombinant wild-type enzyme
34
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
45
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
57
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type, presence of DTT
80.07
N-octanoyl-L-homoserine lactone
-
pH 7.2, 30°C
158
N-octanoyl-L-homoserine lactone
pH 7.1, 25°C
0.08
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
3
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F64C/F68C, absence of DTT
6.1
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F64C/F68C, presence of DTT
43
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
45
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type, presence of DTT
57
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.53
(R)-gamma-caprolactone
pH 8.0, 25°C, recombinant enzyme
2.52
(S)-gamma-caprolactone
pH 8.0, 25°C, recombinant enzyme
280
3-oxo-dodecanoyl-L-homoserine lactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
335
delta-dodecalactone
pH and temperature not specified in the publication
1.4 - 840
delta-nonalactone
580
delta-undecalactone
pH and temperature not specified in the publication
33.47
dodecanoic-delta-lactone
pH 8.0, 25°C, recombinant enzyme
76.19
dodecanoic-gamma-lactone
pH 8.0, 25°C, recombinant enzyme
30.3
Gamma-caprolactone
pH and temperature not specified in the publication
77.7
gamma-dodecalactone
pH and temperature not specified in the publication
31.1
gamma-heptalactone
pH and temperature not specified in the publication
4.3 - 948
gamma-nonalactone
73.6
gamma-undecalactone
pH and temperature not specified in the publication
31.25
heptanoic-gamma-lactone
pH 8.0, 25°C, recombinant enzyme
15.3
L-homoserine lactone
-
pH 8.2, 25°C
290
N-(3-oxodecanoyl)-L-homoserine lactone
pH 8.0, 25°C
14.5
N-(3-oxodecyl)-L-homoserine lactone
-
pH 8.2, 25°C
0.13 - 9.3
N-(3-oxododecanoyl)-L-homoserine lactone
18.1
N-(3-oxododecyl)-L-homoserine lactone
-
pH 8.2, 25°C
0.0045 - 0.093
N-(3-oxohexanoyl)-L-homoserine lactone
0.021 - 6.6
N-(3-oxooctanoyl)-L-homoserine lactone
7.66
N-(3-oxooctyl)-L-homoserine lactone
-
pH 8.2, 25°C
2.04
N-3-oxo-decanoyl-DL-homoserine lactone
pH 8.0, 25°C, recombinant enzyme
0.13 - 9.3
N-3-oxo-dodecanoyl-L-homoserine lactone
0.0045 - 0.045
N-3-oxo-hexanoyl-L-homoserine lactone
1.88
N-3-oxo-octanoyl-DL-homoserine lactone
pH 8.0, 25°C, recombinant enzyme
0.05 - 0.64
N-3-oxo-octanoyl-L-homoserine lactone
0.141 - 4310
N-3-oxododecanoyl-L-homoserine lactone
2.7 - 310
N-3-oxohexanoyl-L-homoserine lactone
1.88 - 450
N-3-oxooctanoyl-L-homoserine lactone
0.0109
N-butanoyl-DL-homoserine lactone
-
pH 7.4, 32°C
1.29 - 10.17
N-butanoyl-L-homoserine lactone
6.35
N-butyryl-DL-homoserine lactone
-
pH 8.2, 25°C
0.0809 - 1300
N-butyryl-L-homoserine lactone
0.103 - 3770
N-decanoyl-L-homoserine lactone
57
N-dodecanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
0.00266
N-heptanoyl-DL-homoserine lactone
-
pH 7.4, 32°C
0.19 - 1700
N-heptanoyl-L-homoserine lactone
0.0119
N-hexanoyl-DL-homoserine lactone
-
pH 7.4, 32°C
0.026 - 2000
N-hexanoyl-L-homoserine lactone
6.35
N-hexyl-L-homoserine lactone
-
pH 8.2, 25°C
0.0117 - 2.25
N-octanoyl-DL-homoserine lactone
0.43 - 540
N-octanoyl-L-homoserine lactone
0.012 - 440
N-pentanoyl-L-homoserine lactone
0.92
N-tetradecanoyl-L-homoserine lactone
-
pH 7.4, 25°C
72.88
nonanoic-delta-lactone
pH 8.0, 25°C, recombinant enzyme
946.6
nonanoic-gamma-lactone
pH 8.0, 25°C, recombinant enzyme
0.0045
paraoxon
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
30.28
rac-gamma-caprolactone
pH 8.0, 25°C, recombinant enzyme
2.55 - 9650
undecanoic-delta-lactone
0.905 - 573
undecanoic-gamma-lactone
450
delta-decalactone
pH 8.3, 25°C
450
delta-decalactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
1.4
delta-nonalactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
1.4
delta-nonalactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
3.9
delta-nonalactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
3.9
delta-nonalactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
72.9
delta-nonalactone
pH and temperature not specified in the publication
840
delta-nonalactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
840
delta-nonalactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
450
delta-valerolactone
pH 8.3, 25°C
450
delta-valerolactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
140
gamma-butyrolactone
pH 8.3, 25°C
140
gamma-butyrolactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
19
gamma-heptanolide
pH 8.3, 25°C
19
gamma-heptanolide
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
4.3
gamma-nonalactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
4.3
gamma-nonalactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
21
gamma-nonalactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
21
gamma-nonalactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
120
gamma-nonalactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
120
gamma-nonalactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
948
gamma-nonalactone
pH and temperature not specified in the publication
0.13
N-(3-oxododecanoyl)-L-homoserine lactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
4.2
N-(3-oxododecanoyl)-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
5.5
N-(3-oxododecanoyl)-L-homoserine lactone
pH 8.0, 25°C
9.3
N-(3-oxododecanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.0045
N-(3-oxohexanoyl)-L-homoserine lactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.017
N-(3-oxohexanoyl)-L-homoserine lactone
-
mutant enzyme E101V/R230T, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.035
N-(3-oxohexanoyl)-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.045
N-(3-oxohexanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.045
N-(3-oxohexanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.093
N-(3-oxohexanoyl)-L-homoserine lactone
pH 8.0, 25°C
0.021
N-(3-oxooctanoyl)-L-homoserine lactone
-
mutant enzyme E101K/R230P, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.05
N-(3-oxooctanoyl)-L-homoserine lactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.11
N-(3-oxooctanoyl)-L-homoserine lactone
-
mutant enzyme E101V/R230T, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.28
N-(3-oxooctanoyl)-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.64
N-(3-oxooctanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.64
N-(3-oxooctanoyl)-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
6.6
N-(3-oxooctanoyl)-L-homoserine lactone
pH 8.0, 25°C
0.13
N-3-oxo-dodecanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
4.2
N-3-oxo-dodecanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
9.3
N-3-oxo-dodecanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
0.0045
N-3-oxo-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
0.035
N-3-oxo-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
0.045
N-3-oxo-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
0.05
N-3-oxo-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
0.28
N-3-oxo-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
0.64
N-3-oxo-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
0.141
N-3-oxododecanoyl-L-homoserine lactone
mutant enzyme C258L/I261F/W263A, at pH 8.0 and 25°C
0.36
N-3-oxododecanoyl-L-homoserine lactone
pH 8.3, 25°C
0.584
N-3-oxododecanoyl-L-homoserine lactone
mutant enzyme L72I/Y99F/I122L/L228M/F229S/W263L, at pH 8.0 and 25°C
1.78
N-3-oxododecanoyl-L-homoserine lactone
mutant enzyme F46L/C258A/W263M/I280T, at pH 8.0 and 25°C
6.67
N-3-oxododecanoyl-L-homoserine lactone
-
pH 7.2, 30°C
31.6
N-3-oxododecanoyl-L-homoserine lactone
wild type enzyme, at pH 8.0 and 25°C
280
N-3-oxododecanoyl-L-homoserine lactone
pH 8.3, 25°C
510
N-3-oxododecanoyl-L-homoserine lactone
pH 7.1, 25°C
4310
N-3-oxododecanoyl-L-homoserine lactone
pH 8.3, 25°C
2.7
N-3-oxohexanoyl-L-homoserine lactone
-
pH 7.4, 25°C
310
N-3-oxohexanoyl-L-homoserine lactone
pH 7.1, 25°C
1.88
N-3-oxooctanoyl-L-homoserine lactone
pH and temperature not specified in the publication
220
N-3-oxooctanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
450
N-3-oxooctanoyl-L-homoserine lactone
pH 7.1, 25°C
1.29
N-butanoyl-L-homoserine lactone
pH 8.3, 25°C
10.17
N-butanoyl-L-homoserine lactone
-
pH 7.2, 30°C
0.0809
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64A
0.112
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206I
0.121
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206V
1.25
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206L
1.5
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64L
4.38
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, wild-type enzyme
5.33
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69I
5.94
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206W
6.51
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69A
7.43
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69F
8.3
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206F
9.88
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W
10.4
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69L
21.9
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/A206F
27
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69W
31.4
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69W/A206F
44.1
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/V69W/A206F
47.7
N-butyryl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/V69W
83
N-butyryl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
83.1
N-butyryl-L-homoserine lactone
pH 8.3, 25°C
160
N-butyryl-L-homoserine lactone
pH 7.1, 25°C
1300
N-butyryl-L-homoserine lactone
pH 8.3, 25°C
1300
N-butyryl-L-homoserine lactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
0.103
N-decanoyl-L-homoserine lactone
pH 8.3, 25°C
0.3
N-decanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
0.3
N-decanoyl-L-homoserine lactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
2.1
N-decanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
3.3
N-decanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
3.3
N-decanoyl-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
3.9
N-decanoyl-L-homoserine lactone
-
pH 7.4, 25°C
6.61
N-decanoyl-L-homoserine lactone
-
pH 7.2, 30°C
13
N-decanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
13
N-decanoyl-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
13.2
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206I
14.3
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64A
20.8
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/V69W/A206F
32.1
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W
70.8
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/V69W
71.4
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206V
75.2
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64L
92
N-decanoyl-L-homoserine lactone
pH 8.3, 25°C
92
N-decanoyl-L-homoserine lactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
94.2
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme F64W/A206F
96.6
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69F
125
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69I
130
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69W/A206F
133
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69W
134
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69L
141
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206L
210
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, wild-type enzyme
210
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme V69A
248
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206F
275
N-decanoyl-L-homoserine lactone
pH and temperature not specified in the publication, mutant enzyme A206W
390
N-decanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
430
N-decanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
3770
N-decanoyl-L-homoserine lactone
pH 8.3, 25°C
0.19
N-heptanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
110
N-heptanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
1700
N-heptanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
0.026
N-hexanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
0.066
N-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
0.066
N-hexanoyl-L-homoserine lactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.068
N-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
0.068
N-hexanoyl-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.082
N-hexanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
0.082
N-hexanoyl-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.169
N-hexanoyl-L-homoserine lactone
pH 8.3, 25°C
3.2
N-hexanoyl-L-homoserine lactone
-
pH 7.4, 25°C
8.81
N-hexanoyl-L-homoserine lactone
-
pH 7.2, 30°C
16
N-hexanoyl-L-homoserine lactone
-
Zn2+-form of AHL lactonase, at pH 7.4 and at 28°C
17
N-hexanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
170
N-hexanoyl-L-homoserine lactone
pH 8.3, 25°C
170
N-hexanoyl-L-homoserine lactone
in 2.5 mM Bicine pH 8.3, 150 mM NaCl, 0.2 mM cresol purple, 0.5% (v/v) DMSO and 0.2 mM CoCl2, at 25°C
290
N-hexanoyl-L-homoserine lactone
pH 7.1, 25°C
970
N-hexanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
1123
N-hexanoyl-L-homoserine lactone
pH 8.3, 25°C
1400
N-hexanoyl-L-homoserine lactone
-
Co2+-form of AHL lactonase, at pH 7.4 and at 28°C
1800
N-hexanoyl-L-homoserine lactone
-
Mn2+-form of AHL lactonase, at pH 7.4 and at 28°C
2000
N-hexanoyl-L-homoserine lactone
-
Cd2+-form of AHL lactonase, at pH 7.4 and at 28°C
0.0117
N-octanoyl-DL-homoserine lactone
-
pH 7.4, 32°C
2.25
N-octanoyl-DL-homoserine lactone
pH 8.0, 25°C, recombinant enzyme
0.43
N-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant wild-type enzyme
0.43
N-octanoyl-L-homoserine lactone
-
wild type enzyme, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
0.58
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
1.2
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F64C/F68C, absence of DTT
2.23
N-octanoyl-L-homoserine lactone
pH and temperature not specified in the publication
3.2
N-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101G/R230C
3.2
N-octanoyl-L-homoserine lactone
-
mutant enzyme E101G/R230C, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
3.7
N-octanoyl-L-homoserine lactone
-
pH 8.3, 37°C, recombinant mutant E101N/R230I
3.7
N-octanoyl-L-homoserine lactone
-
mutant enzyme E101N/R230I, in 2.5 mM Bicine buffer, pH 8.3, at 37°C
7.74
N-octanoyl-L-homoserine lactone
-
pH 7.2, 30°C
32
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F64C/F68C, presence of DTT
81
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type, presence of DTT
190
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
360
N-octanoyl-L-homoserine lactone
pH 7.1, 25°C
540
N-octanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
0.012
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F107W
0.67
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F64C/F68C, absence of DTT
3.4
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, mutant F64C/F68C, presence of DTT
7.5
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type, presence of DTT
14
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication, wild-type
440
N-pentanoyl-L-homoserine lactone
pH 7.5, temperature not specified in the publication
2.55
undecanoic-delta-lactone
wild type enzyme, at pH 8.0 and 25°C
2.55
undecanoic-delta-lactone
mutant enzyme C258L/I261F/W263A, at pH 8.0 and 25°C
5.33
undecanoic-delta-lactone
mutant enzyme V27A/I76T/Y97W/Y99F/L130P/L226V, at pH 8.0 and 25°C
7.5
undecanoic-delta-lactone
mutant enzyme V27A/Y97W/L228M/W263M, at pH 8.0 and 25°C
360
undecanoic-delta-lactone
mutant enzyme L72I/Y99F/I122L/L228M/F229S/W263L, at pH 8.0 and 25°C
578.86
undecanoic-delta-lactone
pH 8.0, 25°C, recombinant enzyme
9650
undecanoic-delta-lactone
mutant enzyme F46L/C258A/W263M/I280T, at pH 8.0 and 25°C
0.905
undecanoic-gamma-lactone
mutant enzyme V27A/I76T/Y97W/Y99F/L130P/L226V, at pH 8.0 and 25°C
1.63
undecanoic-gamma-lactone
mutant enzyme C258L/I261F/W263A, at pH 8.0 and 25°C
2.36
undecanoic-gamma-lactone
wild type enzyme, at pH 8.0 and 25°C
16
undecanoic-gamma-lactone
mutant enzyme V27A/Y97W/L228M/W263M, at pH 8.0 and 25°C
73.83
undecanoic-gamma-lactone
pH 8.0, 25°C, recombinant enzyme
440
undecanoic-gamma-lactone
mutant enzyme F46L/C258A/W263M/I280T, at pH 8.0 and 25°C
573
undecanoic-gamma-lactone
mutant enzyme L72I/Y99F/I122L/L228M/F229S/W263L, at pH 8.0 and 25°C
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V27G/P67Q/L72C/Y97S/Y99A/T177D/R223L/L226Q/L228M/W263H
D108E
site-directed mutagenesis, mutation of a zinc-binding motif residue, the mutant shows 90.8% of wild-type enzyme activity
D108S/H109S
site-directed mutagenesis, mutation of zinc-binding motif residues, inactive mutant
D191S
site-directed mutagenesis, mutation of a zinc-binding motif residue, inactive mutant
D236S
site-directed mutagenesis, the mutation does not affect the enzyme activity
H104L/H106L/D108L
site-directed mutagenesis, mutation of zinc-binding motif residues, the mutant shows 61.4% of wild-type enzyme activity
H104L/H106L/D108L/H109L
site-directed mutagenesis, mutation of zinc-binding motif residues, the mutant shows 37.9% of wild-type enzyme activity
H104S
site-directed mutagenesis, mutation of a zinc-binding motif residue, the mutant is as active as the wild-type enzyme
H104S/H106S
site-directed mutagenesis, mutation of zinc-binding motif residues, the mutant shows 51.1% of wild-type enzyme activity
H104S/H106S/D108S/H109Sc
site-directed mutagenesis, mutation of zinc-binding motif residues, inactive mutant
H104S/H106S/H109S
site-directed mutagenesis, mutation of zinc-binding motif residues, inactive mutant
H169S
-
site-directed mutagenesis, the mutant shows 53.1% of wild-type enzyme activity, and altered secondary structure
H235S
site-directed mutagenesis, the mutation does not affect the enzyme activity
D108S/H109S
-
site-directed mutagenesis, mutation of zinc-binding motif residues, inactive mutant
-
H104L/H106L/D108L/H109L
-
site-directed mutagenesis, mutation of zinc-binding motif residues, the mutant shows 37.9% of wild-type enzyme activity
-
H104S/H106S
-
site-directed mutagenesis, mutation of zinc-binding motif residues, the mutant shows 51.1% of wild-type enzyme activity
-
H104S/H106S/D108S/H109Sc
-
site-directed mutagenesis, mutation of zinc-binding motif residues, inactive mutant
-
H104S/H106S/H109S
-
site-directed mutagenesis, mutation of zinc-binding motif residues, inactive mutant
-
A206W
site-directed mutagenesis, structure and catalytic mechanism compared to the wild-type, overview
D108N
site-directed mutagenesis, structure and catalytic mechanism compared to the wild-type, overview
F107W
crystal structure of AiiA F107W mutant determined in the presence and absence of N-decanoyl- L-homoserine is reported. Mutant shows lower catalytic activity for all substrates tested. Catalytic efficacies (kcat/Km) decrease by 2 to 3 orders of magnitude with N-pentanoyl-L-homoserine lactone displaying the highest decrease
F64C/F68C
in the absence of DTT, the enzymatic activity of F64C/F68C AiiA is impaired as compared to that of the wild-type enzyme, and this reduction is more severe for the long N-octanoyl-L-homoserine lactone substrate than for the short N-pentanoyl-L-homoserine lactone substrate. The kcat value is decreased by 14 and 41fold and the KM by 2 and 4fold, resulting in 21 and 158fold decreases in kcat/KM for the N-pentanoyl-L-homoserine lactone and N-octanoyl-L-homoserine lactone substrates, respectively. The addition of DTT not only partially restores enzyme activity but also recovers the characteristic substrate specificity of the wild-type enzyme
G207D
site-directed mutagenesis, structure and catalytic mechanism compared to the wild-type, overview
G207W
site-directed mutagenesis, structure and catalytic mechanism compared to the wild-type, overview
Y194F
site-directed mutagenesis, structure and catalytic mechanism compared to the wild-type, overview
A206F
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 3.34% compared to 2.09% for wild-type enzyme
A206I
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 0.85% compared to 2.09% for wild-type enzyme
A206L
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 0.89% compared to 2.09% for wild-type enzyme
A206V
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 0.17% compared to 2.09% for wild-type enzyme
A206W
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 2.16% compared to 2.09% for wild-type enzyme
D108A
site-directed mutagenesis, the mutant enzyme shows a reduced Zn2+ content compared to the wild-type enzyme
D191A
site-directed mutagenesis, the mutant enzyme shows a reduced Zn2+ content compared to the wild-type enzyme
F64A
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 0.56% compared to 2.09% for wild-type enzyme
F64I
no activity with N-butyryl-L-homoserine lactone and N-decanoyl-L-homoserine lactone
F64L
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 1.98% compared to 2.09% for wild-type enzyme
F64V
no activity with N-butyryl-L-homoserine lactone and N-decanoyl-L-homoserine lactone
F64W
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 30.71% compared to 2.09% for wild-type enzyme
F64W/A206F
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 23.21% compared to 2.09% for wild-type enzyme
F64W/V69W
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 67.42% compared to 2.09% for wild-type enzyme
F64W/V69W/A206F
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 211.82% compared to 2.09% for wild-type enzyme. The mutant enzyme shows a 100fold shifted preference for N-butyryl-L-homoserine lactone
H109A
site-directed mutagenesis, the mutant enzyme shows a reduced Zn2+ content compared to the wild-type enzyme
H235A
site-directed mutagenesis, the mutant enzyme shows a reduced Zn2+ content compared to the wild-type enzyme
V69A
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 3.1% compared to 2.09% for wild-type enzyme
V69F
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 7.69% compared to 2.09% for wild-type enzyme
V69I
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 4.25% compared to 2.09% for wild-type enzyme
V69L
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 7.72% compared to 2.09% for wild-type enzyme
V69W
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 20.34% compared to 2.09% for wild-type enzyme
V69W/A206F
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 24.23% compared to 2.09% for wild-type enzyme
A206W
-
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 2.16% compared to 2.09% for wild-type enzyme
-
F64A
-
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 0.56% compared to 2.09% for wild-type enzyme
-
F64L
-
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 1.98% compared to 2.09% for wild-type enzyme
-
V69A
-
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 3.1% compared to 2.09% for wild-type enzyme
-
V69F
-
the ratio of kcat/Km for N-butyryl-L-homoserine lactone/N-decanoyl-L-homoserine lactone is 7.69% compared to 2.09% for wild-type enzyme
-
E101K/R230P
-
the mutant shows decreased catalytic efficiency toward 3-oxo-N-octanoyl-L-homoserine lactone and no activioty towards 3-oxo-N-hexanoyl-L-homoserine lactone compared to the wild type enzyme
E101V/R230T
-
the mutant shows increased catalytic efficiency compared to the wild type enzyme
H115W/R192K
site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type
H115W/R192K/A137T
site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type
H115W/R192K/A137T/D94H/S211T
site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type
H115W/R192K/A137T/L130F
site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type
H115W/R192K/A137T/M127I/D263H
site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type
H115W/R192K/A137T/S81R/P165A
site-directed mutagenesis, the mutant shows altered substrate specificity compared to wild-type
L55M
natural polymorphism, the polymorphism at the 55th position of h-PON1 does not affect the catalytic properties of the enzyme
R192E
natural polymorphism, polymorphism at position 192 plays an important role in determining the substrate specificity and catalytic efficiency of the enzyme
E219G
activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 9.1%, activity with substrate N-dodecanoyl-L-homoserine lactone: 25.7%, crystal structure determined at 1.33 A
E219T
the mutant is active at wild type levels
F138G
activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 97.3%, activity with substrate N-dodecanoyl-L-homoserine lactone: 100%
F189G
activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 88.8%, activity with substrate N-dodecanoyl-L-homoserine lactone: 86%
F192G
activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 77.8%, activity with substrate N-dodecanoyl-L-homoserine lactone: 71.7%
F221G
activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 72.7%, activity with substrate N-dodecanoyl-L-homoserine lactone: 77.8%
H248G
mutant shows no activity
L103G
activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 77.2%, activity with substrate N-dodecanoyl-L-homoserine lactone: 78.5%
M144G
activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 98.7%, activity with substrate N-dodecanoyl-L-homoserine lactone: 82.2%
M188G
activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 99.2%, activity with substrate N-dodecanoyl-L-homoserine lactone: 68.2%
M77G
activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 92.2%, activity with substrate N-dodecanoyl-L-homoserine lactone: 80.4%
N33G
activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: 96.5%, activity with substrate N-dodecanoyl-L-homoserine lactone: 92.1%
S102G
mutant shows no activity, crystal structure determined at 1.33 A
Y160G
activity with substrate N-(3-oxo-octanoyl)-L-homoserine lactone: no activity, activity with substrate N-dodecanoyl-L-homoserine lactone: 10.3%
E219T
-
the mutant is active at wild type levels
-
F46L/C258A/W263M/I280T
the mutant exhibits enhanced phosphotriesterase activity compared to the wild type enzyme
L72I/Y99F/I122L/L228M/F229S/W263L
the mutant exhibits enhanced phosphotriesterase activity compared to the wild type enzyme
V27A/I76T/Y97W/Y99F/L130P/L226V
the mutant exhibits enhanced phosphotriesterase activity compared to the wild type enzyme
V27A/Y97W/L228M/W263M
the mutant demonstrates a large increase in catalytic efficiencies over the wild-type enzyme, with increases of 2210fold, 163fold, 58fold, 16fold against methyl parathion, malathion, ethyl paraoxon, and methyl paraoxon, respectively
W263F
site-directed mutagenesis, the W263 residue is previously demonstrated to be involved in the formation of an hydrophobic channel for the substrate leaving group, the mutant enzyme shows decreased lactonase activity compared to the wild-type
V27G/P67Q/L72C/Y97S/Y99A/T177D/R223L/L226Q/L228M/W263H
inactive
V27G/P67Q/L72C/Y97S/Y99A/T177D/R223L/L226Q/L228M/W263H
-
inactive
-
D108S
site-directed mutagenesis, mutation of a zinc-binding motif residue, inactive mutant
D108S
-
site-directed mutagenesis, the mutant shows no activity, and altered secondary structure
H106S
site-directed mutagenesis, mutation of a zinc-binding motif residue, the mutant shows 61.4% of wild-type enzyme activity
H106S
-
site-directed mutagenesis, the mutant shows 53.5% of wild-type enzyme activity, and altered secondary structure
H109S
site-directed mutagenesis, mutation of a zinc-binding motif residue, inactive mutant
H109S
-
site-directed mutagenesis, the mutant shows no activity, and altered secondary structure
D266N
-
inactive
D266N
-
catalytically inactive mutant
E101G/R230C
-
the mutant shows increased catalytic efficiency compared to the wild type enzyme
E101G/R230C
-
the mutant has an increased value of kcat/Km of 72fold toward 3-oxo-N-dodecanoyl-L-homoserine lactone
E101N/R230I
-
the mutant has an increased value of kcat/Km of 72fold toward 3-oxo-N-dodecanoyl-L-homoserine lactone
E101N/R230I
-
the mutant has an 72fold increased kcat/Km value toward 3-oxo-N-dodecanoyl-L-homoserine lactone compared to the wild type enzyme and also exhibits lactonase activity toward N-butyryl-L-homoserine lactone
C258L/I261F/W263A
site-directed mutagenesis, the mutant is not able to hydrolyze C8-HSL or C10-HSL and its paraoxonase activity is 3fold higher than the lactonase activity on 5-thiobutyl butyrolactone, as opposed to the wild-type paraoxonase activity which is 760fold lower than the lactonase activity. The combination of C258L, I261F, and W263A mutations in the SsoPox triple mutant improves the hydrolytic specific activity in terms of kcat/KM toward paraoxon 12fold, the kcat 294fold compared to wild-type SsoPox, while the KM value increases
C258L/I261F/W263A
the mutant exhibits enhanced phosphotriesterase activity compared to the wild type enzyme
W263I
a variant with increased catalytic effectiveness against N-3-oxododecanoyl-L-homoserine lactone
W263I
-
the mutant has increased lactonase and phosphotriesterase activities compared to the wild type
W263I
-
a variant with increased catalytic effectiveness against N-3-oxododecanoyl-L-homoserine lactone
-
additional information
-
induction or mutational derepression of blc as well as a null mutation in blcC have no significant effect on the induction of or continued transfer of pTiC58 from donors in any stage of growth, including stationary phase, overview
additional information
-
expression of AiiA lactonase in Burkholderia thailandensis completely abolishes the accumulation of N-decanoyl-L-homoserine lactone and N-octanoyl-L-homoserine lactone, reduces N-hexanoyl-L-homoserine lactone levels, alters both swarming and twitching motility, causes a significant increase in generation time, and affects carbon metabolism, but the AHL lactonase activity does not enhance beta-hemolytic activity in the transgenic bacteria, overview
additional information
-
expression of AiiA lactonase in Burkholderia thailandensis completely abolishes the accumulation of N-decanoyl-L-homoserine lactone and N-octanoyl-L-homoserine lactone, reduces N-hexanoyl-L-homoserine lactone levels, alters both swarming and twitching motility, causes a significant increase in generation time, and affects carbon metabolism, but the AHL lactonase activity does not enhance beta-hemolytic activity in the transgenic bacteria, overview
-
additional information
-
expression of AiiA lactonase in Burkholderia thailandensis reduces the concentrations of N-decanoyl-L-homoserine lactone, N-octanoyl-L-homoserine lactone, and N-hexanoyl-L-homoserine lactone, alters both swarming and twitching motility, and caused fluctuations in carbon utilization, but the AHL lactonase activity does not enhance beta-hemolytic activity in the transgenic bacteria, overview
additional information
-
AHL-lactonase is produced by overexpression in a zwittermicin A, ZwA, producing strain Bacillus cereus, neither ZwA nor AHL-lactonase impact the yield of the other. Construction of transgenic plants using Agrobacterium tumefaciens strain WCF47 for transfection, the transgenic plants show enhanced pesticide-resistance by combining the two antibacterial agents, overview
additional information
-
expression of AiiA lactonase in Burkholderia thailandensis reduces the concentrations of N-decanoyl-L-homoserine lactone, N-octanoyl-L-homoserine lactone, and N-hexanoyl-L-homoserine lactone, alters both swarming and twitching motility, and caused fluctuations in carbon utilization, but the AHL lactonase activity does not enhance beta-hemolytic activity in the transgenic bacteria, overview
-
additional information
construction of transgenic Solanum tuberosum and Nicotiana tabacum plants via Agrobacterium tumefaciens transfection with Bacillus sp. gene aiiA inserted into their genome, the transgenic plants express the recombinant aiiA gene and show increased AHL-lactonase activity compared to the wild-type plants, as well as enhanced resistance to plant pathogen Erwinia carotovora, the recombinant enzyme might be trapped in microsomes
additional information
-
construction of transgenic Solanum tuberosum and Nicotiana tabacum plants via Agrobacterium tumefaciens transfection with Bacillus sp. gene aiiA inserted into their genome, the transgenic plants express the recombinant aiiA gene and show increased AHL-lactonase activity compared to the wild-type plants, as well as enhanced resistance to plant pathogen Erwinia carotovora, the recombinant enzyme might be trapped in microsomes
additional information
-
construction of transgenic Solanum tuberosum and Nicotiana tabacum plants via Agrobacterium tumefaciens transfection with Bacillus sp. gene aiiA inserted into their genome, the transgenic plants express the recombinant aiiA gene and show increased AHL-lactonase activity compared to the wild-type plants, as well as enhanced resistance to plant pathogen Erwinia carotovora, the recombinant enzyme might be trapped in microsomes
-
additional information
-
construction of an AiiA deficient-mutant to investigate the role of AHL-lactonase for Bacillus thuringiensis and for the protection of pepper roots from root rot
additional information
-
an approach to quorum quenching, rational design of antibacterials in combination with hexahistidine-tagged organophosphorus hydrolase
additional information
the h-PON1 is a polymorphic enzyme, and two polymorphisms are reported in the coding region of the enzyme: one at position 55 (Leu/Met) and the other at position 192 (Arg/Gln). It is observed that the polymorphism at the 55th position of h-PON1 does not affect the catalytic properties of the enzyme, while polymorphism at position 192 plays an important role in determining the substrate specificity and catalytic efficiency of the enzyme. Random mutagenesis approach to increase the organophosphate (OP)-hydrolyzing activity of recombinant h-PON1, and mutant screening for OP-hydrolyzing activity. The mutants show a 10-340fold increased organophosphate-hydrolyzing activity against different organophosphate substrates but also exhibit differential lactonase and arylesterase activities compared to the wild-type
additional information
expression of Microbacterium testaceum AiiM in the plant pathogen Pectobacterium carotovorum subsp. carotovorum reduces the pectinase activity markedly and attenuated soft rot symptoms on Solanum tuberosum tuber slices
additional information
-
expression of Microbacterium testaceum AiiM in the plant pathogen Pectobacterium carotovorum subsp. carotovorum reduces the pectinase activity markedly and attenuated soft rot symptoms on Solanum tuberosum tuber slices
additional information
-
expression of Microbacterium testaceum AiiM in the plant pathogen Pectobacterium carotovorum subsp. carotovorum reduces the pectinase activity markedly and attenuated soft rot symptoms on Solanum tuberosum tuber slices
-
additional information
evolution of a lactonase into a phosphotriesterase, semi-rational engineering approach is used to design an efficient and thermostable organophosphate hydrolase, starting from enzyme SsoPox from Sulfolobus solfataricus as a lactonase scaffold. In particular, by in vitro evolution of the SsoPox ancillary promiscuous activity, the triple mutant C258L/I261F/W263A is obtained which, retaining its inherent stability, shows an enhancement of its hydrolytic activity on paraoxon up to 300fold. The mutant is tested in formulations of different solvents (methanol or ethanol) or detergents (SDS or a commercial soap) for the cleaning of pesticide-contaminated surfaces. Construction of a chimeric gene ssopox-pte by insertion of 16 conserved residues of pte gene in the ssopox sequence. Recombination by DNA StEP between ssopox-pte chimera and ssopox gene
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Dong, Y.H.; Gusti, A.R.; Zhang, Q.; Xu, J.L.; Zhang, L.H.
Identification of quorum-quenching N-acyl homoserine lactonases from Bacillus species
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1754-1759
2002
Bacillus cereus, Bacillus mycoides, Bacillus sp. (in: Bacteria) (Q8RPW9), Bacillus sp. (in: Bacteria), Bacillus thuringiensis (A3FJ64), Bacillus thuringiensis (Q8RJA0), Bacillus thuringiensis (Q8RPW5), Bacillus thuringiensis (Q8RPW6), Bacillus thuringiensis, no activity in Bacillus fusiformis, no activity in Bacillus sphaericus
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Lee, S.J.; Park, S.Y.; Lee, J.J.; Yum, D.Y.; Koo, B.T.; Lee, J.K.
Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis
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Bacillus thuringiensis serovar kurstaki (P0CJ63), Bacillus thuringiensis (Q7B8C0), Bacillus thuringiensis (Q7B8C1), Bacillus thuringiensis (Q7B8C2), Bacillus thuringiensis (Q7B8C3), Bacillus thuringiensis (Q7B8C4), Bacillus thuringiensis (Q7B8C5), Bacillus thuringiensis (Q8KI79), Bacillus thuringiensis (Q8KTW4), Bacillus thuringiensis (Q8KTW5), Bacillus thuringiensis (Q8KTW6), Bacillus thuringiensis (Q8KTW7), Bacillus thuringiensis (Q8KTW8), Bacillus thuringiensis (Q8KTW9), Bacillus thuringiensis (Q8KTX0), Bacillus thuringiensis (Q8KTX1), Bacillus sp. (in: Bacteria) (Q9L8R8), Bacillus sp. (in: Bacteria) IBN35 (Q9L8R8)
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The Ti plasmid of Agrobacterium tumefaciens harbors an attM-paralogous gene, aiiB, also encoding N-acyl homoserine lactonase activity
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4989-4993
2003
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
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Ulrich, R.L.
Quorum quenching: Enzymatic disruption of N-acylhomoserine lactone-mediated bacterial communication in Burkholderia thailandensis
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2004
Bacillus anthracis, Bacillus cereus, Bacillus anthracis Ames, Bacillus cereus A24
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Wopperer, J.; Cardona, S.T.; Huber, B.; Jacobi, C.A.; Valvano, M.A.; Eberl, L.
A quorum-quenching approach to investigate the conservation of quorum-sensing-regulated functions within the Burkholderia cepacia complex
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2006
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) 240B1
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Thomas, P.W.; Stone, E.M.; Costello, A.L.; Tierney, D.L.; Fast, W.
The quorum-quenching lactonase from Bacillus thuringiensis is a metalloprotein
Biochemistry
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2005
Bacillus thuringiensis serovar kurstaki (P0CJ63), Bacillus thuringiensis serovar kurstaki 4A3 (P0CJ63), Bacillus thuringiensis serovar kurstaki BGSC 4A3 (P0CJ63)
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Bacillus thuringiensis serovar kurstaki (P0CJ63)
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Bos taurus, Bos taurus aiiA, Capra hircus, Capra hircus aiiA, Equus caballus, Equus caballus aiiA, Homo sapiens, Homo sapiens aiiA, Mus musculus, Mus musculus aiiA, no activity in Gallus gallus, Oryctolagus cuniculus, Oryctolagus cuniculus aiiA
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Wang, L.H.; Weng, L.X.; Dong, Y.H.; Zhang, L.H.
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Bacillus sp. (in: Bacteria)
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AhlD, an N-acylhomoserine lactonase in Arthrobacter sp., and predicted homologues in other bacteria
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Arthrobacter sp. (Q7X3T2), Arthrobacter sp., Klebsiella pneumoniae (Q7X477), Klebsiella pneumoniae, Arthrobacter sp. IBN110 (Q7X3T2), Klebsiella pneumoniae KCTC 2241 / ATCC 25306 (Q7X477)
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Acyl-homoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum-quenching enzymes
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2003
Ralstonia sp.
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Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase
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Bacillus sp. (in: Bacteria) (Q9L8R8), Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) 240B1 (Q9L8R8)
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Three-dimensional structure of the quorum-quenching N-acyl homoserine lactone hydrolase from Bacillus thuringiensis
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Bacillus thuringiensis serovar kurstaki (P0CJ63)
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The molecular structure and catalytic mechanism of a quorum-quenching N-acyl-L-homoserine lactone hydrolase
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Bacillus thuringiensis serovar kurstaki (P0CJ63)
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AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora
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Bacillus sp. (in: Bacteria) (Q9L8R8), Bacillus sp. (in: Bacteria) 240B1 (Q9L8R8)
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Quorum quenching activity in Anabaena sp. PCC 7120: identification of AiiC, a novel AHL-acylase
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Anabaena sp. (Q8YQA9)
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Rhodococcus corynebacterioides, Rhodococcus corynebacterioides CBCT240, Rhodococcus erythropolis (Q5DQU3), Rhodococcus erythropolis, Rhodococcus rhodochrous, Rhodococcus rhodochrous CECT3042
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Bacillus thuringiensis (P0CJ63), Bacillus thuringiensis
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Bacillus cereus
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Bacillus thuringiensis (P0CJ63)
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Agrobacterium tumefaciens
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Bacillus thuringiensis
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2010
Microbacterium testaceum (C6L862), Microbacterium testaceum, Microbacterium testaceum StLB037 (C6L862), Microbacterium testaceum StLB037
brenda
Augustine, N.; Kumar, P.; Thomas, S.
Inhibition of Vibrio cholerae biofilm by AiiA enzyme produced from Bacillus spp.
Arch. Microbiol.
192
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2010
Bacillus sp. B6 (D9IVB6), Bacillus sp. B10 (D9IVB7)
brenda
Seo, M.J.; Lee, B.S.; Pyun, Y.R.; Park, H.
Isolation and characterization of N-acylhomoserine lactonase from the Thermophilic bacterium, Geobacillus caldoxylosilyticus YS-8
Biosci. Biotechnol. Biochem.
75
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2011
Parageobacillus caldoxylosilyticus, Parageobacillus caldoxylosilyticus YS-8
brenda
Chow, J.Y.; Xue, B.; Lee, K.H.; Tung, A.; Wu, L.; Robinson, R.C.; Yew, W.S.
Directed evolution of a thermostable quorum-quenching lactonase from the amidohydrolase superfamily
J. Biol. Chem.
285
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2010
Geobacillus kaustophilus
brenda
Bijtenhoorn, P.; Schipper, C.; Hornung, C.; Quitschau, M.; Grond, S.; Weiland, N.; Streit, W.
BpiB05, a novel metagenome-derived hydrolase acting on N-acylhomoserine lactones
J. Biotechnol.
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86-94
2011
uncultured bacterium (B2BKC0)
brenda
Fan, J.; Qian, G.; Yang, X.; Gu, C.; Kang, Y.; Ma, Y.; Hu, B.; Liu, F.
Biocontrol of bacterial soft rot of calla lily by elicitor HarpinXoo and N-acyl homoserine lactonase (AttM)
World J. Microbiol. Biotechnol.
27
401-410
2011
Agrobacterium tumefaciens
brenda
Mei, G.Y.; Yan, X.X.; Turak, A.; Luo, Z.Q.; Zhang, L.Q.
AidH, an alpha/beta-hydrolase fold family member from an Ochrobactrum sp. strain, is a novel N-acylhomoserine lactonase
Appl. Environ. Microbiol.
76
4933-4942
2010
Ochrobactrum sp. T63 (D2J2T6), Ochrobactrum sp. T63 ATCC 49188 (D2J2T6)
brenda
Thomas, P.W.; Fast, W.
Heterologous overexpression, purification, and in vitro characterization of AHL lactonases
Methods Mol. Biol.
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2011
Bacillus thuringiensis
brenda
Chen, R.; Zhou, Z.; Cao, Y.; Bai, Y.; Yao, B.
High yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromonas hydrophila mortality in aquaculture
Microb. Cell Fact.
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39
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Bacillus thuringiensis serovar kyushuensis (D8VDY4), Bacillus thuringiensis serovar kyushuensis B546 (D8VDY4)
brenda
Ng, F.S.; Wright, D.M.; Seah, S.Y.
Characterization of a phosphotriesterase-like lactonase from Sulfolobus solfataricus and its immobilization for disruption of quorum sensing
Appl. Environ. Microbiol.
77
1181-1186
2010
Saccharolobus solfataricus (Q97VT7), Saccharolobus solfataricus, Saccharolobus solfataricus P2 (Q97VT7)
brenda
Sirotkina, M.; Efremenko, E.N.
Rhodococcus lactonase with organophosphate hydrolase (OPH) activity and His6-tagged OPH with lactonase activity: evolutionary proximity of the enzymes and new possibilities in their application
Appl. Microbiol. Biotechnol.
98
2647-2656
2014
Brevundimonas diminuta, Rhodococcus ruber, Rhodococcus erythropolis, Rhodococcus ruber AC-1513D, Rhodococcus erythropolis AC-1514D
brenda
Charendoff, M.; Shah, H.; Briggs, J.
New insights into the binding and catalytic mechanisms of Bacillus thuringiensis lactonase: insights into B. thuringiensis AiiA mechanism
PLoS ONE
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e75395
2013
Bacillus thuringiensis
brenda
Gao, A.; Mei, G.Y.; Liu, S.; Wang, P.; Tang, Q.; Liu, Y.P.; Wen, H.; An, X.M.; Zhang, L.Q.; Yan, X.X.; Liang, D.C.
High-resolution structures of AidH complexes provide insights into a novel catalytic mechanism for N-acyl homoserine lactonase
Acta Crystallogr. Sect. D
69
82-91
2013
Ochrobactrum sp. T63 (D2J2T6)
brenda
Pedroza, C.; Flrez, A.; Ruiz, O.; Orduz, S.
Enzymatic hydrolysis of molecules associated with bacterial quorum sensing using an acyl homoserine lactonase from a novel Bacillus thuringiensis strain
Antonie van Leeuwenhoek
105
253-264
2014
Bacillus thuringiensis
brenda
Ochiai, S.; Yasumoto, S.; Morohoshi, T.; Ikeda, T.
AmiE, a novel N-acylhomoserine lactone acylase belonging to the amidase family, from the activated sludge isolate Acinetobacter sp. Ooi24
Appl. Environ. Microbiol.
80
6919-6925
2014
Acinetobacter sp.
brenda
Liu, C.F.; Liu, D.; Momb, J.; Thomas, P.W.; Lajoie, A.; Petsko, G.A.; Fast, W.; Ringe, D.
A phenylalanine clamp controls substrate specificity in the quorum-quenching metallo-gamma-lactonase from Bacillus thuringiensis
Biochemistry
52
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2013
Bacillus thuringiensis (A3FJ64), Bacillus thuringiensis
brenda
Vinoj, G.; Vaseeharan, B.; Thomas, S.; Spiers, A.; Shanthi, S.
Quorum-quenching activity of the AHL-lactonase from Bacillus licheniformis DAHB1 inhibits Vibrio biofilm formation in vitro and reduces shrimp intestinal colonisation and mortality
Mar. Biotechnol.
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2014
Bacillus licheniformis
brenda
Wang, W.Z.; Morohoshi, T.; Someya, N.; Ikeda, T.
Diversity and distribution of N-acylhomoserine lactone (AHL)-degrading activity and AHL-lactonase (AiiM) in genus microbacterium
Microbes Environ.
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2012
Microbacterium sp., Microbacterium testaceum
brenda
Hanano, A.; Harba, M.; Al-Ali, M.; Ammouneh, H.
Silencing of Erwinia amylovora sy69 AHL-quorum sensing by a Bacillus simplex AHL-inducible aiiA gene encoding a zinc-dependent N-acyl-homoserine lactonase
Plant Pathol.
63
773-783
2014
Peribacillus simplex
brenda
Ghani, N.A.; Norizan, S.N.; Chan, X.Y.; Yin, W.F.; Chan, K.G.
Labrenzia sp. BM1: a quorum quenching bacterium that degrades N-acyl homoserine lactones via lactonase activity
Sensors
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2014
Labrenzia sp. BM1
brenda
Ghani, N.; Sulaiman, J.; Ismail, Z.; Chan, X.; Yin, W.; Chan, K.
Rhodotorula Mucilaginosa, a quorum quenching yeast exhibiting lactonase activity isolated from a tropical shoreline
Sensors
14
6463-6473
2014
Rhodotorula mucilaginosa
brenda
Bergonzi, C.; Schwab, M.; Elias, M.
The quorum-quenching lactonase from Geobacillus caldoxylosilyticus purification, characterization, crystallization and crystallographic analysis
Acta Crystallogr. Sect. F
72
681-686
2016
Parageobacillus caldoxylosilyticus (A0A023DFE8), Parageobacillus caldoxylosilyticus, Parageobacillus caldoxylosilyticus NBRC 107762 (A0A023DFE8)
brenda
Bergonzi, C.; Schwab, M.; Chabriere, E.; Elias, M.
The quorum-quenching lactonase from Alicyclobacter acidoterrestris purification, kinetic characterization, crystallization and crystallographic analysis
Acta Crystallogr. Sect. F
73
476-480
2017
Alicyclobacillus acidoterrestris (T0BMH6)
brenda
Tripathy, R.K.; Aggarwal, G.; Bajaj, P.; Kathuria, D.; Bharatam, P.V.; Pande, A.H.
Towards understanding the catalytic mechanism of human paraoxonase 1 experimental and in silico mutagenesis studies
Appl. Biochem. Biotechnol.
182
1642-1662
2017
Homo sapiens (P27169)
brenda
Tang, K.; Su, Y.; Brackman, G.; Cui, F.; Zhang, Y.; Shi, X.; Coenye, T.; Zhang, X.
MomL, a novel marine-derived N-Acyl homoserine lactonase from Muricauda olearia
Appl. Environ. Microbiol.
81
774-782
2015
Muricauda olearia (A0A0A1EIU0), Muricauda olearia, Muricauda olearia Th120 (A0A0A1EIU0)
brenda
Sakr, M.M.; Aboshanab, K.M.; Elkhatib, W.F.; Yassien, M.A.; Hassouna, N.A.
Overexpressed recombinant quorum quenching lactonase reduces the virulence, motility and biofilm formation of multidrug-resistant Pseudomonas aeruginosa clinical isolates
Appl. Microbiol. Biotechnol.
102
10613-10622
2018
Bacillus mycoides (R4UZR2), Bacillus mycoides
brenda
Kyeong, H.; Kim, J.; Kim, H.
Design of N-acyl homoserine lactonase with high substrate specificity by a rational approach
Appl. Microbiol. Biotechnol.
99
4735-4742
2015
Bacillus thuringiensis serovar kurstaki (P0CJ63), Bacillus thuringiensis serovar kurstaki HD-263 (P0CJ63)
brenda
Mayer, C.; Romero, M.; Muras, A.; Otero, A.
Aii20J, a wide-spectrum thermostable N-acylhomoserine lactonase from the marine bacterium Tenacibaculum sp. 20J, can quench AHL-mediated acid resistance in Escherichia coli
Appl. Microbiol. Biotechnol.
99
9523-9539
2015
Tenacibaculum sp. 20J (A0A0H3YS61)
brenda
Mascarenhas, R.; Thomas, P.W.; Wu, C.X.; Nocek, B.P.; Hoang, Q.Q.; Liu, D.; Fast, W.
Structural and biochemical characterization of AidC, a quorum-quenching lactonase with atypical selectivity
Biochemistry
54
4342-4353
2015
Chryseobacterium sp. StRB126 (I7HB71)
brenda
Aslanli, A.; Lyagin, I.; Efremenko, E.
Novel approach to quorum quenching rational design of antibacterials in combination with hexahistidine-tagged organophosphorus hydrolase
Biol. Chem.
399
869-879
2018
Escherichia coli
brenda
Del Giudice, I.; Coppolecchia, R.; Merone, L.; Porzio, E.; Carusone, T.M.; Mandrich, L.; Worek, F.; Manco, G.
An efficient thermostable organophosphate hydrolase and its application in pesticide decontamination
Biotechnol. Bioeng.
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724-734
2016
Saccharolobus solfataricus (Q97VT7)
brenda
Shastry, R.P.; Dolan, S.K.; Abdelhamid, Y.; Vittal, R.R.; Welch, M.
Purification and characterisation of a quorum quenching AHL-lactonase from the endophytic bacterium Enterobacter sp. CS66
FEMS Microbiol. Lett.
365
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Enterobacter sp. CS66
brenda
Fan, X.; Liang, M.; Wang, L.; Chen, R.; Li, H.; Liu, X.
Aii810, a novel cold-adapted N-acylhomoserine lactonase discovered in a metagenome, can strongly attenuate Pseudomonas aeruginosa virulence factors and biofilm formation
Front. Microbiol.
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1950
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uncultured bacterium (A0A286S6I1)
brenda
Guendouze, A.; Plener, L.; Bzdrenga, J.; Jacquet, P.; Remy, B.; Elias, M.; Lavigne, J.P.; Daude, D.; Chabriere, E.
Effect of quorum quenching lactonase in clinical isolates of Pseudomonas aeruginosa and comparison with quorum sensing inhibitors
Front. Microbiol.
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227
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Saccharolobus solfataricus (Q97VT7), Saccharolobus solfataricus DSM 1617 (Q97VT7)
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Rajesh, P.S.; Rai, V.R.
Purification and antibiofilm activity of AHL-lactonase from endophytic Enterobacter aerogenes VT66
Int. J. Biol. Macromol.
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1046-1052
2015
Klebsiella aerogenes, Klebsiella aerogenes VT66P
brenda
Gui, M.; Wu, R.; Liu, L.; Wang, S.; Zhang, L.; Li, P.
Effects of quorum quenching by AHL lactonase on AHLs, protease, motility and proteome patterns in Aeromonas veronii LP-11
Int. J. Food Microbiol.
252
61-68
2017
Bacillus sp. AI96 (G9B7C4)
brenda
Easwaran, N.; Karthikeyan, S.; Sridharan, B.; Gothandam, K.
Identification and analysis of the salt tolerant property of AHL lactonase (AiiATSAWB) of Bacillus species
J. Basic Microbiol.
55
579-590
2015
Bacillus sp. TSAWB (T2KSW9)
brenda
Morohoshi, T.; Tominaga, Y.; Someya, N.; Ikeda, T.
Characterization of a novel thermostable N-acylhomoserine lactonase from the thermophilic bacterium Thermaerobacter marianensis
J. Biosci. Bioeng.
120
1-5
2015
Bacillus cereus, Thermaerobacter marianensis, Thermaerobacter nagasakiensis (A0A060NR36), Thermaerobacter nagasakiensis, Thermaerobacter composti (A0A060NTE1), Solibacillus silvestris (F8WSM9), Solibacillus silvestris, Chryseobacterium sp. StRB126 (I7HB71), Bacillus cereus ATCC 14579, Solibacillus silvestris StLB046 (F8WSM9), Thermaerobacter nagasakiensis JCM 11223 (A0A060NR36), Thermaerobacter marianensis JCM 10246, Thermaerobacter composti JCM 15650 (A0A060NTE1)
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Morohoshi, T.; Kamimura, Y.; Sato, N.; Iizumi, T.
Distribution and characterization of N-acylhomoserine lactone (AHL)-degrading activity and AHL lactonase gene (qsdS) in Sphingopyxis
J. Biosci. Bioeng.
127
411-417
2018
Sphingopyxis alaskensis, Sphingopyxis bauzanensis, Sphingomonas ursincola, Sphingopyxis sp. EG6 (A0A2Z5U8E0), Sphingopyxis sp. FD7 (A0A2Z5UR29), Sphingopyxis bauzanensis DSM 22271, Sphingopyxis alaskensis DSM 13593
brenda
Cai, X.; Yu, M.; Shan, H.; Tian, X.; Zheng, Y.; Xue, C.; Zhang, X.H.
Characterization of a novel N-acylhomoserine lactonase RmmL from Ruegeria mobilis YJ3
Mar. Drugs
16
E370
2018
Tritonibacter mobilis, Tritonibacter mobilis YJ3
brenda
See-Too, W.S.; Convey, P.; Pearce, D.A.; Chan, K.G.
Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp
Microb. Cell Fact.
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179
2018
Planococcus versutus, Planococcus versutus L10.15T
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Liu, W.; Ran, C.; Liu, Z.; Gao, Q.; Xu, S.; Ringo, E.; Myklebust, R.; Gu, Z.; Zhou, Z.
Effects of dietary Lactobacillus plantarum and AHL lactonase on the control of Aeromonas hydrophila infectionin tilapia
MicrobiologyOpen
5
687-699
2016
Ochrobactrum sp. M231 (J9PRP0)
brenda
Rhoads, M.K.; Hauk, P.; Gupta, V.; Bookstaver, M.L.; Stephens, K.; Payne, G.F.; Bentley, W.E.
Modification and assembly of a versatile lactonase for bacterial quorum quenching
Molecules
23
E341
2018
Saccharolobus solfataricus
brenda
Garge, S.S.; Nerurkar, A.S.
Attenuation of quorum sensing regulated virulence of Pectobacterium carotovorum subsp. carotovorum through an AHL lactonase produced by Lysinibacillus sp. Gs50
PLoS ONE
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e0167344
2016
Lysinibacillus sp. (A0A142LT01), Lysinibacillus sp. Gs50 (A0A142LT01)
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Hiblot, J.; Bzdrenga, J.; Champion, C.; Chabriere, E.; Elias, M.
Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia
Sci. Rep.
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8372
2015
Vulcanisaeta moutnovskia (F0QXN6), Vulcanisaeta moutnovskia, Vulcanisaeta moutnovskia 768-28 (F0QXN6)
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Remy, B.; Plener, L.; Poirier, L.; Elias, M.; Daude, D.; Chabriere, E.
Harnessing hyperthermostable lactonase from Sulfolobus solfataricus for biotechnological applications
Sci. Rep.
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37780
2016
Saccharolobus solfataricus
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Jacquet, P.; Hiblot, J.; Daude, D.; Bergonzi, C.; Gotthard, G.; Armstrong, N.; Chabriere, E.; Elias, M.
Rational engineering of a native hyperthermostable lactonase into a broad spectrum phosphotriesterase
Sci. Rep.
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16745
2017
Saccharolobus solfataricus (Q97VT7)
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Torres, M.; Uroz, S.; Salto, R.; Fauchery, L.; Quesada, E.; Llamas, I.
HqiA, a novel quorum-quenching enzyme which expands the AHL lactonase family
Sci. Rep.
7
943
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uncultured bacterium
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Bergonzi, C.; Schwab, M.; Naik, T.; Daude, D.; Chabriere, E.; Elias, M.
Structural and biochemical characterization of AaL, a quorum quenching lactonase with unusual kinetic properties
Sci. Rep.
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11262
2018
Alicyclobacillus acidoterrestris (T0BMH6), Alicyclobacillus acidoterrestris, Alicyclobacillus acidoterrestris DSM 3922 (T0BMH6)
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Dong, W.; Zhu, J.; Guo, X.; Kong, D.; Zhang, Q.; Zhou, Y.; Liu, X.; Zhao, S.; Ruan, Z.
Characterization of AiiK, an AHL lactonase, from Kurthia huakui LAM0618T and its application in quorum quenching on Pseudomonas aeruginosa PAO1
Sci. Rep.
8
6013
2018
Kurthia huakuii, Kurthia huakuii LAM0618T
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Hiblot, J.; Bzdrenga, J.; Champion, C.; Chabriere, E.; Elia, M.
Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia
Sci. Rep.
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8372
2015
Vulcanisaeta moutnovskia (F0QXN6), Vulcanisaeta moutnovskia
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