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1-naphthyl acetate + H2O
1-naphthol + acetate
1-naphthyl butyrate + H2O
1-naphthol + butyrate
2-naphthyl acetate + H2O
2-naphthol + acetate
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
4-methylumbelliferyl butyrate + H2O
4-methylumbelliferone + butyrate
4-methylumbelliferyl propionate + H2O
4-methylumbelliferone + propionate
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
-
-
-
?
carboxyfluorescein diacetate + H2O
?
-
-
-
-
?
carboxyfluorescin diacetate + H2O
?
-
-
-
-
?
diethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + diethyl phosphate
-
-
-
-
?
dimethyl 4-nitrophenyl phosphate + H2O
4-nitrophenol + dimethyl phosphate
-
-
-
-
?
naphthyl acetate + H2O
naphthol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
p-nitrophenyl butyrate + H2O
p-nitrophenol + butyrate
-
22°C, pH 7.4, no substrate inhibition below 1 mM p-nitrophenyl butyrate
quantification at 405 nm
-
?
S-formylglutathione + H2O
formate + glutathione
-
-
-
-
?
S-lactoylglutathione + H2O
lactone + glutathione
-
-
quantification at 240 nm
-
?
additional information
?
-
1-naphthyl acetate + H2O
1-naphthol + acetate
-
phenotype Es-B, but not Es-D
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
phenotype Es-B, but not Es-D
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
phenotype Es-B, but not Es-D
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
-
?
1-naphthyl butyrate + H2O
1-naphthol + butyrate
-
phenotype Es-B, but not Es-D
-
-
?
1-naphthyl butyrate + H2O
1-naphthol + butyrate
-
phenotype Es-B, but not Es-D
-
-
?
1-naphthyl butyrate + H2O
1-naphthol + butyrate
-
phenotype Es-B, but not Es-D
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
phenotype Es-D
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
phenotype Es-D
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
phenotype Es-D
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
esterase D is identical to sialic acid-specific O-acetylesterase, which appears to be involved in the recycling of O-acetylated sialic acid molecules
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
hydrolysis via formation of covalent, negatively charged enzyme-substrate tetrahydral intermediate: nucleophilic attack of S149 on carbonyl moiety after activation by H260, intermediate stabilized in oxyanion hole by M150 and L54, H260 stabilized by D226, release of alcohol followed by nucleophilic attack of water molecule at the acidic moiety and release of acetate
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
2 min, 25°C, pH 7.4
reaction stop by citric acid, quantification at 460 nm
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
-
?
4-methylumbelliferyl butyrate + H2O
4-methylumbelliferone + butyrate
-
two electrophoretic phenotypes: Es-B and Es-D
-
-
?
4-methylumbelliferyl butyrate + H2O
4-methylumbelliferone + butyrate
-
two electrophoretic phenotypes: Es-B and Es-D
-
-
?
4-methylumbelliferyl butyrate + H2O
4-methylumbelliferone + butyrate
-
two electrophoretic phenotypes: Es-B and Es-D
-
-
?
4-methylumbelliferyl butyrate + H2O
4-methylumbelliferone + butyrate
-
-
-
-
?
4-methylumbelliferyl propionate + H2O
4-methylumbelliferone + propionate
-
Es-D
-
-
?
4-methylumbelliferyl propionate + H2O
4-methylumbelliferone + propionate
-
Es-D
-
-
?
4-methylumbelliferyl propionate + H2O
4-methylumbelliferone + propionate
-
Es-D
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
22°C, pH 7.4, no substrate inhibition below 5 mM p-nitrophenyl acetate
quantification at 405 nm
-
?
additional information
?
-
-
acts on short chain acyl esters of 4-methylumbelliferone, but not on naphthyl, indoxyl or thiocholine esters
-
-
?
additional information
?
-
-
the enzyme catalyses the hydrolysis of S-formylglutathione, a role in formaldehyde detoxification is possible
-
-
?
additional information
?
-
-
preference of p-nitrophenyl acetate over p-nitrophenyl butyrate reversed upon mutation W197I
-
-
?
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4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
additional information
?
-
-
the enzyme catalyses the hydrolysis of S-formylglutathione, a role in formaldehyde detoxification is possible
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
phenotype Es-D
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
phenotype Es-D
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
phenotype Es-D
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
esterase D is identical to sialic acid-specific O-acetylesterase, which appears to be involved in the recycling of O-acetylated sialic acid molecules
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
hydrolysis via formation of covalent, negatively charged enzyme-substrate tetrahydral intermediate: nucleophilic attack of S149 on carbonyl moiety after activation by H260, intermediate stabilized in oxyanion hole by M150 and L54, H260 stabilized by D226, release of alcohol followed by nucleophilic attack of water molecule at the acidic moiety and release of acetate
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
-
?
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0.86 - 3.1
1-naphthyl acetate
2.9 - 4
2-naphthyl acetate
0.067
4-Methylumbelliferyl butyrate
-
pH 5.2, 37°C, isoenzymes Es D1 and Es D2
0.9
4-nitrophenyl acetate
-
pH 5.2, 37°C, isoenzymes Es D1 and Es D2
0.55 - 0.83
4-nitrophenyl butyrate
0.01 - 0.076
4-yethylumbelliferyl acetate
0.056
carboxyfluorescin diacetate
-
pH 7.0, 37°C
1.7
Naphthyl acetate
-
pH 6.0, 23°C
0.013 - 2.6
p-nitrophenyl acetate
0.012 - 0.14
p-nitrophenyl butyrate
0.88
S-formylglutathione
-
pH 7.0, 30°C
additional information
p-nitrophenyl butyrate
0.86
1-naphthyl acetate
-
pH 7.0, 37°C
2.5
1-naphthyl acetate
-
pH 5.2, 37°C, isoenzyme Es D1
3.1
1-naphthyl acetate
-
pH 5.2, 37°C, isoenzyme Es D2
2.9
2-naphthyl acetate
-
pH 5.2, 37°C, isoenzyme Es D2
4
2-naphthyl acetate
-
pH 5.2, 37°C, isoenzyme Es D1
0.55
4-nitrophenyl butyrate
-
pH 5.2, 37°C, isoenzyme Es D1n
0.83
4-nitrophenyl butyrate
-
pH 5.2, 37°C, isoenzyme Es D2
0.01
4-yethylumbelliferyl acetate
-
pH 6.0, 23°C
0.022
4-yethylumbelliferyl acetate
-
25°C, isoenzymes Es D1 and Es D2
0.03
4-yethylumbelliferyl acetate
-
pH 5.2, 37°C, isoenzymes Es D1 and Es D2
0.076
4-yethylumbelliferyl acetate
-
pH 7.0, 37°C
0.013
p-nitrophenyl acetate
-
mutant C60R/W197I
0.054
p-nitrophenyl acetate
-
mutant W197I
0.1
p-nitrophenyl acetate
-
mutant C60Q/W197I
0.14
p-nitrophenyl acetate
-
mutant C60S/W197I
0.15
p-nitrophenyl acetate
-
mutant C60K/W197I
0.19
p-nitrophenyl acetate
-
mutant C60S
0.23
p-nitrophenyl acetate
-
mutant L58F/W197I, Lineweaver-Burke extrapolation
0.32
p-nitrophenyl acetate
-
mutant C60S, after removal of beta-mercaptoethanol
0.34
p-nitrophenyl acetate
-
mutant C60H/W197I
0.35
p-nitrophenyl acetate
-
pH 7.0, 37°C
0.4
p-nitrophenyl acetate
-
wild-type
0.9
p-nitrophenyl acetate
-
mutant M162H
2.6
p-nitrophenyl acetate
-
mutant M162H/C60S/W197I
0.012
p-nitrophenyl butyrate
-
mutant C60Q/W197I
0.018
p-nitrophenyl butyrate
-
mutant C60R/W197I
0.019
p-nitrophenyl butyrate
-
mutant C60S/W197I
0.03
p-nitrophenyl butyrate
-
mutant C60K/W197I
0.04
p-nitrophenyl butyrate
-
mutant W197I
0.09
p-nitrophenyl butyrate
-
wild-type
0.09
p-nitrophenyl butyrate
-
mutant C60S
0.12
p-nitrophenyl butyrate
-
mutant M162H/C60S/W197I
0.13
p-nitrophenyl butyrate
-
mutant C60H/W197I
0.14
p-nitrophenyl butyrate
-
mutant M162H
3
S-Lactoylglutathione
-
mutant C60S/W197I
3
S-Lactoylglutathione
-
mutant W197I
additional information
p-nitrophenyl butyrate
-
below 0.2 mM, mutant L58F/W197I, Lineweaver-Burke extrapolation
additional information
S-Lactoylglutathione
-
above 1.25 mM, wild-type
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0.0067 - 0.767
diethyl 4-nitrophenyl phosphate
0.00483 - 0.8
dimethyl 4-nitrophenyl phosphate
0.0055 - 1.783
p-nitrophenyl acetate
0.0032 - 3.63
p-nitrophenyl butyrate
50 - 116.7
S-Lactoylglutathione
additional information
dimethyl p-nitrophenyl phosphate
0.0067
diethyl 4-nitrophenyl phosphate
-
phosphorylation rate, wild-type, 22°C
0.1
diethyl 4-nitrophenyl phosphate
-
phosphorylation rate, mutant W197H, 22°C
0.187
diethyl 4-nitrophenyl phosphate
-
phosphorylation rate, mutant W197I, 22°C
0.317
diethyl 4-nitrophenyl phosphate
-
phosphorylation rate, double mutant W197I/C60Q, 22°C
0.333
diethyl 4-nitrophenyl phosphate
-
phosphorylation rate, double mutant W197I/C60H, 22°C
0.733
diethyl 4-nitrophenyl phosphate
-
phosphorylation rate, double mutant W197I/C60R, 22°C
0.767
diethyl 4-nitrophenyl phosphate
-
phosphorylation rate, double mutant W197I/C60K, 22°C
0.00483
dimethyl 4-nitrophenyl phosphate
-
phosphorylation rate, wild-type, 22°C
0.217
dimethyl 4-nitrophenyl phosphate
-
phosphorylation rate, mutant M162H, 22°C
0.24
dimethyl 4-nitrophenyl phosphate
-
phosphorylation rate, double mutant W197I/C60Q, 22°C
0.417
dimethyl 4-nitrophenyl phosphate
-
phosphorylation rate, mutant W197I, 22°C
0.53
dimethyl 4-nitrophenyl phosphate
-
phosphorylation rate, double mutant W197I/C60H, 22°C
0.637
dimethyl 4-nitrophenyl phosphate
-
phosphorylation rate, double mutant W197I/C60K, 22°C
0.8
dimethyl 4-nitrophenyl phosphate
-
phosphorylation rate, double mutant W197I/C60R, 22°C
0.0055
p-nitrophenyl acetate
-
mutant L58F/W197I, Lineweaver-Burke extrapolation
0.0283
p-nitrophenyl acetate
-
mutant M162H/C60S/W197I
0.1
p-nitrophenyl acetate
-
mutant M162H
0.317
p-nitrophenyl acetate
-
mutant C60R/W197I
0.525
p-nitrophenyl acetate
-
mutant C60Q/W197I
0.763
p-nitrophenyl acetate
-
mutant C60S/W197I
0.972
p-nitrophenyl acetate
-
mutant C60K/W197I
1.05
p-nitrophenyl acetate
-
mutant W197I
1.5
p-nitrophenyl acetate
-
mutant C60H/W197I
1.53
p-nitrophenyl acetate
-
mutant C60S
1.583
p-nitrophenyl acetate
-
mutant C60S, after removal of beta-mercaptoethanol
1.783
p-nitrophenyl acetate
-
wild-type
0.0032
p-nitrophenyl butyrate
-
mutant M162H
0.04
p-nitrophenyl butyrate
-
mutant M162H/C60S/W197I
0.078
p-nitrophenyl butyrate
-
mutant C60S
0.198
p-nitrophenyl butyrate
-
wild-type
0.43
p-nitrophenyl butyrate
-
mutant C60R/W197I
1.23
p-nitrophenyl butyrate
-
mutant C60K/W197I
1.65
p-nitrophenyl butyrate
-
mutant C60Q/W197I
1.917
p-nitrophenyl butyrate
-
mutant W197I
1.97
p-nitrophenyl butyrate
-
mutant C60S/W197I
3.63
p-nitrophenyl butyrate
-
mutant C60H/W197I
50
S-Lactoylglutathione
-
mutant C60S/W197I
116.7
S-Lactoylglutathione
-
mutant W197I
additional information
dimethyl p-nitrophenyl phosphate
-
no detectable phosphorylation rate, double mutant W197I/C60S, 22°C
additional information
p-nitrophenyl acetate
-
mutant C60H/W197I, catalytic efficiency kcat/KM: 260 +/-20 1/mM/min
additional information
p-nitrophenyl acetate
-
mutant C60K/W197I, catalytic efficiency kcat/KM: 390 +/-30 1/mM/min
additional information
p-nitrophenyl acetate
-
mutant C60Q/W197I, catalytic efficiency kcat/KM: 320 +/-30 1/mM/min
additional information
p-nitrophenyl acetate
-
mutant C60R/W197I, catalytic efficiency kcat/KM: 1500 +/-200 1/mM/min
additional information
p-nitrophenyl acetate
-
mutant C60S, after removal of beta-mercaptoethanol, catalytic efficiency kcat/KM: 300 +/-60 1/mM/min
additional information
p-nitrophenyl acetate
-
mutant C60S, catalytic efficiency kcat/KM: 480 +/-80 1/mM/min
additional information
p-nitrophenyl acetate
-
mutant C60S/W197I, catalytic efficiency kcat/KM: 330 +/-20 1/mM/min
additional information
p-nitrophenyl acetate
-
mutant L58F/W197I, Lineweaver-Burke extrapolation, catalytic efficiency kcat/KM: 1.5 +/-0.5 1/mM/min
additional information
p-nitrophenyl acetate
-
mutant M162H, catalytic efficiency kcat/KM: 7 +/-3 1/mM/min
additional information
p-nitrophenyl acetate
-
mutant M162H/C60S/W197I, catalytic efficiency kcat/KM: 0.7 +/-0.2 1/mM/min
additional information
p-nitrophenyl acetate
-
mutant W197I, catalytic efficiency kcat/KM: 1200 +/-100 1/mM/min
additional information
p-nitrophenyl acetate
-
wild-type, catalytic efficiency kcat/KM: 270 +/-50 1/mM/min
additional information
p-nitrophenyl butyrate
-
maximal 0.0165/sec, mutant L58F/W197I, Lineweaver-Burke extrapolation
additional information
p-nitrophenyl butyrate
-
mutant C60H/W197I, catalytic efficiency kcat/KM: 1700 +/-300 1/mM/min
additional information
p-nitrophenyl butyrate
-
mutant C60K/W197I, catalytic efficiency kcat/KM: 2500 +/-1600 1/mM/min
additional information
p-nitrophenyl butyrate
-
mutant C60Q/W197I, catalytic efficiency kcat/KM: 8000 +/-1000 1/mM/min
additional information
p-nitrophenyl butyrate
-
mutant C60R/W197I, catalytic efficiency kcat/KM: 1400 +/-200 1/mM/min
additional information
p-nitrophenyl butyrate
-
mutant C60S, catalytic efficiency kcat/KM: 50 +/-20 1/mM/min
additional information
p-nitrophenyl butyrate
-
mutant C60S/W197I, catalytic efficiency kcat/KM: 6200 +/-700 1/mM/min
additional information
p-nitrophenyl butyrate
-
mutant L58F/W197I, Lineweaver-Burke extrapolation, catalytic efficiency kcat/KM: below 5 1/mM/min
additional information
p-nitrophenyl butyrate
-
mutant M162H, catalytic efficiency kcat/KM: 1.4 +/-0.5 1/mM/min
additional information
p-nitrophenyl butyrate
-
mutant M162H/C60S/W197I, catalytic efficiency kcat/KM: 20 +/-5 1/mM/min
additional information
p-nitrophenyl butyrate
-
mutant W197I, catalytic efficiency kcat/KM: 2900 +/-700 1/mM/min
additional information
p-nitrophenyl butyrate
-
wild-type, catalytic efficiency kcat/KM: 130 +/-30 1/mM/min
additional information
S-Lactoylglutathione
-
mutant C60S/W197I, catalytic efficiency kcat/KM: 1000 +/-700 1/mM/min
additional information
S-Lactoylglutathione
-
mutant W197I, catalytic efficiency kcat/KM: 2000 +/-1000 1/mM/min
additional information
S-Lactoylglutathione
-
wild-type, activity not twice above background (spontaneous hydrolysis)
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additional information
Diethyl p-nitrophenyl phosphate
additional information
Diethyl p-nitrophenyl phosphate
-
double mutant W197I/C60H, spontaneous dephosphorylation below 1%, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
double mutant W197I/C60K, spontaneous dephosphorylation below 2%, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
double mutant W197I/C60Q, spontaneous dephosphorylation below 5%, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
double mutant W197I/C60R, spontaneous dephosphorylation below 1%, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
half maximal phosphorylation at inhibitor concentration of 0.38 +/-0.05 mM, mutant W197H, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
half maximal phosphorylation at inhibitor concentration of 0.6 +/-0.2 mM, double mutant W197I/C60K, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
half maximal phosphorylation at inhibitor concentration of 0.7 +/-0.1 mM, mutant W197I, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
half maximal phosphorylation at inhibitor concentration of 0.8 +/-0.6 mM, double mutant W197I/C60H, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
half maximal phosphorylation at inhibitor concentration of 1.04 +/-0.09 mM, double mutant W197I/C60R, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
half maximal phosphorylation at inhibitor concentration of 1.1 +/-0.3 mM, double mutant W197I/C60Q, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
half maximal phosphorylation at inhibitor concentration of 29.9 +/-9 mM, wild-type, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
mutant W197I, spontaneous dephosphorylation below 4%, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
rate of enzyme-inhibitor complex formation in absence of substrate: 0.014 +/-0.005 mM/h, wild-type, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
rate of enzyme-inhibitor complex formation in absence of substrate: 16 +/-2 mM/h, mutants W197I and W197H, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
rate of enzyme-inhibitor complex formation in absence of substrate: 17 +/-5 mM/h, double mutant W197I/C60Q, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
rate of enzyme-inhibitor complex formation in absence of substrate: 30 +/-20 mM/h, double mutant W197I/C60H, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
rate of enzyme-inhibitor complex formation in absence of substrate: 42 +/-4 mM/h, double mutant W197I/C60R, 22°C
additional information
Diethyl p-nitrophenyl phosphate
-
rate of enzyme-inhibitor complex formation in absence of substrate: 80 +/-30 mM/h, double mutant W197I/C60K, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
double mutant W197I/C60H, spontaneous dephosphorylation rate: 0.09 1/h, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
double mutant W197I/C60K, spontaneous dephosphorylation rate: 0.24 1/h, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
double mutant W197I/C60Q, spontaneous dephosphorylation rate: 0.25 1/h, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
double mutant W197I/C60R, spontaneous dephosphorylation rate: 0.15 1/h, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
double mutant W197I/C60S, spontaneous dephosphorylation rate: 0.17 1/h, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
half maximal phosphorylation at inhibitor concentration of 2.3 +/-0.3 mM, double mutant W197I/C60K, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
half maximal phosphorylation at inhibitor concentration of 2.5 +/-0.9 mM, wild-type, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
half maximal phosphorylation at inhibitor concentration of 200 +/-100 mM, mutant M162H, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
half maximal phosphorylation at inhibitor concentration of 3.5 +/-0.8 mM, mutant W197I, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
half maximal phosphorylation at inhibitor concentration of 4 +/-2 mM, double mutant W197I/C60H, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
half maximal phosphorylation at inhibitor concentration of 5 +/-1 mM, double mutant W197I/C60Q, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
half maximal phosphorylation at inhibitor concentration of 5 +/-1 mM, double mutant W197I/C60R, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
mutant M162H, spontaneous dephosphorylation rate: 0.20 1/h, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
mutant W197I, spontaneous dephosphorylation rate: 0.4 1/h, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
rate of enzyme-inhibitor complex formation in absence of substrate: 0.07 +/-0.05 mM/h, mutant M162H, 22°C
additional information
dimethyl p-nitrophenyl phosphate
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rate of enzyme-inhibitor complex formation in absence of substrate: 0.12 +/-0.04 mM/h, wild-type, 22°C
additional information
dimethyl p-nitrophenyl phosphate
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rate of enzyme-inhibitor complex formation in absence of substrate: 10 +/-2 mM/h, double mutant W197I/C60R, 22°C
additional information
dimethyl p-nitrophenyl phosphate
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rate of enzyme-inhibitor complex formation in absence of substrate: 12.9 +/-0.6 mM/h, double mutant W197I/C60Q, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
rate of enzyme-inhibitor complex formation in absence of substrate: 17 +/-2 mM/h, double mutant W197I/C60K, 22°C
additional information
dimethyl p-nitrophenyl phosphate
-
rate of enzyme-inhibitor complex formation in absence of substrate: 7 +/-2 mM/h, mutant W197I, 22°C
additional information
dimethyl p-nitrophenyl phosphate
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rate of enzyme-inhibitor complex formation in absence of substrate: 8 +/-4 mM/h, double mutant W197I/C60H, 22°C
additional information
dimethyl p-nitrophenyl phosphate
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wild-type, spontaneous dephosphorylation rate: 0.8 1/h, 22°C
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hanging drop: 16°C, 3 days, reservoir solution: sodium citrate pH 5.6, 200 mM ammonium acetate, 30% (w/v) polyethylene glycol (PEG) 4000, crystal: space group P2(1), unit cell parameters: a: 51.54, b: 70.72, c: 65.01, alpha: 90°, beta: 108.84°, gamma: 90°, 2 molecules in the asymmetric unit, molecular replacement using PDB: 1PV1, co-crystallization and soaking with substrate failed, structure: several insertions in canonical alpha/beta-hydrolase fold, GxSxG motif, active site in positively charged cleft including residues S149, D226, and H260, and aromatic residues (H148, F172 W183, F228, H260, Y262) lined at the surface, catalysis mediated by residues S153, H264, and D230, oxyanion hole formed by e.g. L54 and M150
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apo-enzyme (PDB: 1PV1) and mutant W197I (PDB: 3C6B) following inhibition through phosphorylation with diethyl p-nitrophenyl phosphate, sitting drop: pH 4.6, 25.5% (w/v) polyethylene glycol (PEG) 4000, 15% glycerol, 17°C, crystals: space group: P2(1)2(1)2(1) for PDB: 1PV1 (four molecules in asymmetric unit, dimer of dimers), C2 for PDB: 3C6B, structure: PDB: 1PV1: three-layer sandwich without disulfide bonds, catalytic triad: S161, H276, D241 and adjacent C60, oxyanion hole: L58 and M162, PDB: 3C6B: tetrahedral shape, sulfenic acid at residue C60, structural superimposition of wild-type and mutant W197I: natural resistance to paraoxon (diethyl p-nitrophenyl phosphate, an organophosphorus inhibitor) due to steric hindrance by W197 within the enzymes acyl pocket (L58, W102, I189, F243, L248, W197)
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D226A
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4.3% of wild-type activity
D226N
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9% of wild-type activity
H260A
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3% of wild-type activity
H260Q
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1.3% of wild-type activity
L54A
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83% of wild-type activity
M150
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162% of wild-type activity, possibly due to reduced steric hindrance
S149A
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catalytically inactive
S149T
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1.3% of wild-type activity
C60H/W197I
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increased kcat and similar KM for p-nitrophenyl substrates compared to mutant W197I, increased phoshorylation by diethyl p-nitrophenyl phosphate
C60K/W197I
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similar kcat and KM for p-nitrophenyl substrates compared to mutant W197I, increased phoshorylation by diethyl p-nitrophenyl phosphate, 11°C decreased melting temperature compared to mutant W197I
C60Q/W197I
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decreased catalytic activity, but no affect on KM for p-nitrophenyl acetate compared to mutant W197I
C60R/W197I
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similar kcat and KM for p-nitrophenyl substrates compared to mutant W197I, increased phoshorylation by diethyl p-nitrophenyl phosphate, 10°C decreased melting temperature compared to mutant W197I
C60S/W197I
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double mutant
G57H
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decreased catalytic activity, enhanced dephosphorylation rate, close to oxyanion hole
G57H/W197I
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catalytically inactive
L58H
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decreased catalytic activity, far from oxyanion hole
M162H
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similar catalytic activity like wild-type, far from oxyanion hole
M162H/C60S/W197I
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triple mutant
W197H
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comparable to mutant W197I
W197I
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p-nitrophenyl butyrate preferred over p-nitrophenyl acetate as substrate, increased specificity towards and turnover of p-nitrophenyl butyrate and S-lactoylglutathione, enhanced organophosphate inhibition under pseudo-first-order conditions, no enhanced organophosphate hydrolysis, similar spontaneous dephosphorylation rate like wild-type, deprotonated at residue C60 leads to formation of a sulfenic acid
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Homo sapiens
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Homo sapiens
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83
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Homo sapiens
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83
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Homo sapiens
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-
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