Information on EC 3.1.1.2 - arylesterase

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The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria

EC NUMBER
COMMENTARY hide
3.1.1.2
-
RECOMMENDED NAME
GeneOntology No.
arylesterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a phenyl acetate + H2O = a phenol + acetate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
non-pathway related
-
-
Bisphenol degradation
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
aryl-ester hydrolase
Acts on many phenolic esters. The reactions of EC 3.1.8.1 aryldialkylphosphatase, were previously attributed to this enzyme. It is likely that the three forms of human paraoxonase are lactonases rather than aromatic esterases [7,8]. The natural substrates of the paraoxonases are lactones [7,8], with (+/-)-5-hydroxy-6E,8Z,11Z,4Z-eicostetraenoic-acid 1,5-lactone being the best substrate [8].
CAS REGISTRY NUMBER
COMMENTARY hide
9032-73-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
2 populations, one of which, Rothamsted, is suceptible to herbicides, while the other, Peldon, shows cross-resistance to multiple classes of herbicides
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-
Manually annotated by BRENDA team
strain DSM 4304
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
strains S587 and S5019
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-
Manually annotated by BRENDA team
strains E109, S5080 and S5079
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
enzyme associated with resistance to methyl-parathion
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Huebner, pyrethroid-resistant strain
-
-
Manually annotated by BRENDA team
Huebner, pyrethroid-resistant strain
-
-
Manually annotated by BRENDA team
LILA, enzyme EstB, gene estB
SwissProt
Manually annotated by BRENDA team
enzyme EstA, gene estA
SwissProt
Manually annotated by BRENDA team
enzyme EstA, gene estA
SwissProt
Manually annotated by BRENDA team
lipase/esterase
UniProt
Manually annotated by BRENDA team
strains U510, LL30, LL32 and CH38
-
-
Manually annotated by BRENDA team
parsley
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
baker's yeast
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-
Manually annotated by BRENDA team
chimeric mammalian dubbed G3C9-8His, obtained using directed evolution via gene shuffling of human, rabbit, rat and mouse genes
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-
Manually annotated by BRENDA team
cv. Hunter
-
-
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-naphthyl acetate + H2O
1-naphthol + acetate
show the reaction diagram
1-phenylvinyl acetate + H2O
1-phenylethylenol + acetate
show the reaction diagram
-
-
-
-
?
2,2,2-trifluoroethyl acetate + H2O
2,2,2-trifluoroethanol + acetate
show the reaction diagram
-
-
-
-
?
2,2-difluoroethyl acetate + H2O
2,2-difluoroethanol + acetate
show the reaction diagram
-
-
-
-
?
2,3-difluorophenyl acetate + H2O
2,3-difluorophenol + acetate
show the reaction diagram
-
-
-
-
?
2,4-difluorophenyl acetate + H2O
2,4-difluorophenol + acetate
show the reaction diagram
-
-
-
-
?
2,6-difluorophenyl acetate + H2O
2,6-difluorophenol + acetate
show the reaction diagram
-
-
-
-
?
2-carboxythiophenyl acetate + H2O
2-carboxythiophenol + acetate
show the reaction diagram
-
-
-
?
2-fluoroethyl acetate + H2O
2-fluoroethanol + acetate
show the reaction diagram
-
-
-
-
?
2-fluorophenyl acetate + H2O
2-fluorophenol + acetate
show the reaction diagram
-
-
-
-
?
2-methoxythiophenyl acetate + H2O
2-methoxythiophenol + acetate
show the reaction diagram
-
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
show the reaction diagram
2-naphthyl butanoate + H2O
2-naphthol + butanoate
show the reaction diagram
-
-
-
?
2-napthyl acetate + H2O
2-naphthol + acetate
show the reaction diagram
-
-
-
?
2-nitrophenyl acetate + H2O
2-nitrophenol + acetate
show the reaction diagram
3,4-dimethyl phenyl acetate + H2O
3,4-dimethylphenol + acetate
show the reaction diagram
-
-
-
-
?
3-acetoxypyridine + H2O
?
show the reaction diagram
-
-
?
3-cyanophenyl acetate + H2O
3-cyanophenol + acetate
show the reaction diagram
-
-
-
-
?
3-fluorophenyl acetate + H2O
3-fluorophenol + acetate
show the reaction diagram
-
-
-
-
?
3-naphthyl acetate + H2O
3-naphthol + acetate
show the reaction diagram
-
-
-
?
3-naphthyl butanoate + H2O
3-naphthol + butanoate
show the reaction diagram
-
-
-
?
3-nitrophenyl acetate + H2O
3-nitrophenol + acetate
show the reaction diagram
-
-
-
-
?
4-(chloromethyl)phenyl acetate + H2O
4-chloromethyl phenol + acetate
show the reaction diagram
-
-
-
-
?
4-acetoxyacetophenone + H2O
4-hydroxyacetophenone + acetate
show the reaction diagram
-
-
-
-
?
4-acetoxybenzaldehyde + H2O
4-hydroxybenzaldehyde + acetate
show the reaction diagram
-
-
-
-
?
4-acetoxybenzaldehyde + H2O
?
show the reaction diagram
4-carboxyphenyl acetate + H2O
4-carboxyphenol + acetate
show the reaction diagram
-
-
-
?
4-carboxythiophenyl acetate + H2O
4-carboxythiophenol + acetate
show the reaction diagram
-
-
-
?
4-chlorophenyl acetate + H2O
4-chlorophenol + acetate
show the reaction diagram
-
-
-
-
?
4-cyanophenyl acetate + H2O
4-cyanophenol + acetate
show the reaction diagram
-
-
-
-
?
4-methoxy phenyl acetate + H2O
4-methoxy phenol + acetate
show the reaction diagram
-
-
-
-
?
4-methylphenyl acetate + H2O
4-methylphenol + acetate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate
4-nitrophenol + acetate
show the reaction diagram
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
4-nitrophenyl acetate + H2O
phenol + acetate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl butanoate + H2O
4-nitrophenol + butanoate
show the reaction diagram
4-nitrophenyl butyrate
4-nitrophenol + butyrate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
show the reaction diagram
4-nitrophenyl caprate + H2O
4-nitrophenol + caprate
show the reaction diagram
-
-
-
?
4-nitrophenyl caproate + H2O
4-nitrophenol + caproate
show the reaction diagram
4-nitrophenyl caprylate + H2O
4-nitrophenol + caprylate
show the reaction diagram
4-nitrophenyl decanoate
4-nitrophenol + decanoate
show the reaction diagram
-
low activity
-
-
?
4-nitrophenyl decanoate + H2O
4-nitrophenol + decanoate
show the reaction diagram
-
-
?
4-nitrophenyl diethylphosphate + H2O
diethylphosphate + 4-nitrophenol
show the reaction diagram
4-nitrophenyl hexanoate + H2O
4-nitrophenol + hexanoate
show the reaction diagram
4-nitrophenyl laurate + H2O
4-nitrophenol + laurate
show the reaction diagram
4-nitrophenyl myristate + H2O
4-nitrophenol + myristate
show the reaction diagram
about 10% of the activity with 4-nitrophenyl acetate
-
-
?
4-nitrophenyl octanoate + H2O
4-nitrophenol + octanoate
show the reaction diagram
-
-
?
4-nitrophenyl palmitate + H2O
4-nitrophenol + palmitate
show the reaction diagram
4-nitrophenyl pentanoate + H2O
4-nitrophenol + pentanoate
show the reaction diagram
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
show the reaction diagram
4-nitrophenyl valerate + H2O
4-nitrophenol + valerate
show the reaction diagram
-
-
?
9-(4-chlorophenyloxycarbonyl)-10-methylacridinium triflate + H2O
9-carboxy-10-methylacridinium triflate + 4-chlorophenol
show the reaction diagram
-
-
-
-
?
acetylcholine chloride + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
show the reaction diagram
benzyl acetate + H2O
benzyl alcohol + acetate
show the reaction diagram
-
-
-
-
?
beta-naphthyl acetate + H2O
beta-naphthol + acetate
show the reaction diagram
-
-
-
-
?
binapacryl + H2O
?
show the reaction diagram
bromoxynil octanoate + H2O
?
show the reaction diagram
butyl acetate + H2O
butanol + acetate
show the reaction diagram
-
-
-
-
?
butyrylcholine iodide + H2O
?
show the reaction diagram
-
-
-
-
?
diclofop methyl + H2O
?
show the reaction diagram
ethyl acetate + H2O
ethanol + acetate
show the reaction diagram
-
-
-
-
?
ethyl hexanoate + H2O
ethanol + hexanoate
show the reaction diagram
-
-
?
isopropyl acetate + H2O
isobutanol + acetate
show the reaction diagram
-
-
-
-
?
ketoprofen ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
methyl 4-(acetyloxy)benzoate + H2O
methyl 4-hydroxybenzoate + acetate
show the reaction diagram
-
-
-
-
?
mipafox + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl butyrate + H2O
p-nitrophenol + butanoate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl propionate + H2O
p-nitrophenol + propanoate
show the reaction diagram
-
-
-
-
?
phenyl acetate + H2O
?
show the reaction diagram
phenyl acetate + H2O
phenol + acetate
show the reaction diagram
phenyl valerate + H2O
phenol + valerate
show the reaction diagram
-
-
-
-
?
phenylacetate + H2O
phenol + acetate
show the reaction diagram
phenylthioacetate + H2O
thiophenol + acetate
show the reaction diagram
propioncholine iodide + H2O
?
show the reaction diagram
-
-
-
-
?
propyl acetate + H2O
propanol + acetate
show the reaction diagram
-
-
-
-
?
S-methyl thiobutanoate + H2O
methanol + thiobutanoate
show the reaction diagram
-
-
-
?
thiophenyl acetate + H2O
?
show the reaction diagram
thiophenyl acetate + H2O
phenol + thioacetate
show the reaction diagram
-
-
-
-
?
thiophenyl acetate + H2O
thiophenol + acetate
show the reaction diagram
-
-
-
?
tributyrin + H2O
dibutyrin + butyrate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phenyl acetate + H2O
?
show the reaction diagram
phenylacetate + H2O
phenol + acetate
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
-
added to assay
Co2+
catalytic activity is strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+)
Mg2+
-
slightly acitvating
Ni2+
catalytic activity is strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+)
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-hydroxyquinoline
2-mercaptoethanol
2-propanol
55% residual activity in the presence of 90% (v/v) 2-propanol, after 60 min at 70C
4-chloromercuribenzoate
6-palmityl-ascorbic acid
-
-
acetonitrile
43% residual activity in the presence of 90% (v/v) acetonitrile, after 60 min at 70C
Ag+
9.7% residual activity at 5 mM
ascorbate
-
0.5 mM inhibits by ca. 19%. Ascorbate/Cu2+ (0.5 mM/0.001 mM) system shows ca. 91% inactivation
butylated hydroxytoluene
-
-
Cr3+
39.8% residual activity at 5 mM
diethyl dicarbonate
diethyldicarbonate
0.5 mM, complete loss of activity
diisopropyl fluorophosphate
diisopropylfluorophosphate
dimethyl sulfoxide
71% residual activity in the presence of 90% (v/v) dimethyl sulfoxide, after 60 min at 70C
dimyristoylphosphatidic acid
-
0.4 mM, 81% inhibition of arylesterase activity, 64% inhibition of paraoxonase activity
dimyristoylphosphatidylserine
-
no significant inhibition of both paraoxonase and arylesterase activity up to 0.030 mM, remarkable inhibition of both activities at 0.10.4 mM, with a greater inhibition of arylesterase activity
dithiothreitol
19.4% residual activity at 5 mM
eserine
ethanol
Fe3+
13.7% residual activity at 5 mM
high density lipoprotein
-
incubation of serum or high density lipoprotein from healthy subjects with very low density lipoprotein significantly decreases serum paraoxonase 1 lactonase or arylesterase activities by up to 11% or 24%, and HDL-associated paraoxonase 1 lactonase or arylesterase activities by up to 32% or 46%, respectively
-
iodoacetic acid
slight inhibition
lanthanum(III) chloride
-
about 50% inhibition at 0.05 mM
methanol
48% residual activity in the presence of 90% (v/v) methanol, after 60 min at 70C
methylglyoxal
-
decreases specific activity in a concentration-dependent manner
metrifonate
-
50% inhibition at 0.00048 mM, pralidoxime protects
N-ethylmaleimide
-
leads to 24fold reduced enzyme activity at 0.2 mM
nitrilotriacetic acid
-
-
organophosphorous compounds
-
inhibit the enzyme of all genotypes slightly after in vivo exposure
-
p-chloromercuribenzoate
p-hydroxymercuribenzoate
Paraoxon
pepstatin A
phenyl acetate
Phenylglyoxal
phenylmethylsulfonyl fluoride
phosphatidylserine
-
-
piperonyl butoxide
-
50% inhibition at 0.6 mM. Enzyme inhibition in vivo reaches a maximum after 4 h and enzyme subsequently recovers over a 24 h period
pyridoxal 5'-phosphate
0.5 mM, 62% residual activity
sodium dodecylsulfate
1 mM, 32% of initial activity
sodium hypochlorite
-
at 1 mM causes approximately 15% decrease in activity, at 1 mM and in the presence of PBS buffer causes approximately 76% decrease in activity
Triton X-100
Tween 80
21% residual activity in the presence of 5% (v/v) Tween 80, after 60 min at 70C
Valproic acid
-
arylesterase activity is decreased after 60 days of valproic acid treatment
very low density lipoprotein
-
inhibits recombinant paraoxonase 1 lactonase or arylesterase activities by up to 20% or 42%, respectively
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-dilauroyl-phosphatidylcholine
-
stimulation
1-palmitoyl-2-arachidonoylphosphatidylcholine
-
stimulates arylesterase activity
1-palmitoyl-2-linoleoylphosphatidylcholine
-
stimulates arylesterase activity
3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate
the enzyme is activated by 87% by incubation with 1% (w/v) 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate, at 30C
aspirin
-
induces enzyme expression in the liver and stimulates enzymatic activity in blood plasma
Ca2+
-
required
cardiolipin
-
stimulates
cyclophosphamide
-
22% increase in the activity of PON1 in the liver and 23% increase in PON1 activity in the serum is observed 24 h after treatment with a single dose of 150 mg/kg cyclophosphamide
cysteine
1 mM, 117% of initial activity
di-oleoyl phosphatidylcholine
-
2.6 mM di-oleoyl phosphatidylcholine stimulates arylesterase activity of PON1 3.5fold
didecanoylphosphatidylcholine
-
stimulation of arylesterase activity is greater than stimulation of paraoxonase activity
dilauroyl phosphatidylcholine
dilauroylphosphatidylcholine
-
stimulation of arylesterase activity is greater than stimulation of paraoxonase activity
dilinoleoylphosphatidylcholine
-
stimulates arylesterase activity
dimethyl sulfoxide
21% increase of activity at 50% (v/v) dimethyl sulfoxide, at 30C
dimyristoyl phosphatidylcholine
dimyristoylphosphatidylcholine
-
stimulation of arylesterase activity is greater than stimulation of paraoxonase activity
dimyristoylphosphatidylglycerol
-
activates the arylesterase activity and inhibits paraoxonase activity
dioleoyl phosphatidylcholine
dipalmitoylphosphatidylcholine
-
stimulation of arylesterase activity is greater than stimulation of paraoxonase activity
ethanol
high-density lipoprotein
-
paraoxonase and arylesterase activities are stimulated by high-density lipoprotein by 2-5fold, almost independently of the apoliporotein content
-
lecithin
Lubrol
the enzyme is activated by 14% by incubation with 1% Lubrol, at 30C
-
lysolecithin
lysophosphatidylinositol
-
0.01 mM, 10% stimulation of arylesterase activity
Mg2+
-
Mg2, Mn2+ and other divalent metal ions can not substitute for Ca2+ and lead to a loss od arylesterase activty
Mn2+
-
Mg2, Mn2+ and other divalent metal ions can not substitute for Ca2+ and lead to a loss od arylesterase activty
monooleoyl-lysophosphatidylserine
-
0.01 mM, 8% stimulation of arylesterase activity
Nonidet P-40
8.2% activation at 5% (v/v)
palmitoyl-lysophosphatidylglycerol
-
0.01 mM, 47% stimulation of arylesterase activity
Paraoxon
115 increase of activita in the presence of 0.5 mM paraoxon, after 30 min at 30C
phosphate
phosphatidylcholine
-
purified A or B-type esterases are stimulated
phosphatidylethanolamine
phosphatidylglycerol
phosphatidylinositol
-
enhances arylesterase activity, but slightly decreases paraoxonase activity
phosphatidylsenine
-
stimulates
-
pyridoxal 5'-phosphate
110% activity in the presence of 5 mM pyridoxal 5'-phosphate, after 30 min at 30C
salicylic acid
-
induces enzyme expression in the liver and stimulates enzymatic activity in blood plasma
sphingomyelin
-
stimulates
Triton X-100
Tween 20
Tween 40
16.4% activation at 5% (v/v)
Tween 80
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.041
1-naphthyl acetate
in 50 mM Tris-HCl (pH 8.0), at 37C
5
1-phenylvinyl acetate
-
-
19
2,2,2-trifluoroethyl acetate
-
-
24.8
2,2-difluoroethyl acetate
-
-
1.19
2,3-difluorophenyl acetate
-
-
2.23
2,4-difluorophenyl acetate
-
-
2
2,6-difluorophenyl acetate
-
-
54
2-fluoroethyl acetate
-
-
1.5
2-fluorophenyl acetate
-
-
0.0079 - 0.65
2-naphthyl acetate
0.0128
2-naphthyl butanoate
pH 7.0, 25
2.1
3,4-dimethyl phenyl acetate
-
-
1.9
3-cyanophenyl acetate
-
-
1.4
3-fluorophenyl acetate
-
-
0.0052
3-naphthyl acetate
pH 7.0, 25
0.0088
3-naphthyl butanoate
pH 7.0, 25
2.2
3-nitrophenyl acetate
-
-
2
4-acetoxy methyl benzoate
-
-
2.6
4-acetoxyacetophenone
-
-
2.3
4-acetoxybenzaldehyde
1
4-chlorophenyl acetate
-
-
4.1
4-methoxy phenyl acetate
-
-
0.000043 - 8.19
4-nitrophenyl acetate
0.174 - 0.38
4-nitrophenyl butanoate
0.224
4-nitrophenyl butyrate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
0.081
4-nitrophenyl caprate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
0.031
4-nitrophenyl caproate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
0.028
4-nitrophenyl caprylate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
0.084
4-nitrophenyl decanoate
EstB, pH 7.5, 30C
0.029
4-nitrophenyl hexanoate
EstB, pH 7.5, 30C
0.122
4-nitrophenyl laurate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
0.23
4-nitrophenyl octanoate
EstB, pH 7.5, 30C
0.276
4-nitrophenyl palmitate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
0.063
4-nitrophenyl pentanoate
EstB, pH 7.5, 30C
0.234
4-nitrophenyl phosphate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
1.4
4-nitrophenyl propionate
EstB, pH 7.5, 30C
0.029 - 0.19
Alpha-naphthyl acetate
5
Benzyl acetate
-
-
2.1
beta-naphthyl acetate
-
-
10
butyl acetate
-
-
15.4
ethyl acetate
-
-
3.2
ethyl hexanoate
EstB, pH 7.5, 30C
1.6
ketoprofen ethyl ester
-
at pH 8.0
0.043 - 0.29
p-nitrophenyl acetate
1.26
p-tolyl acetate
-
-
1.42
Paraoxon
-
pH 7.4, 25C
0.01 - 16
phenyl acetate
5.5
phenylthioacetate
EstB, pH 7.5, 30C
13
propyl acetate
-
-
7.2
S-methyl thiobutanoate
pH 8.0, 25
-
additional information
9-(4-chlorophenyloxycarbonyl)-10-methylacridinium triflate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
1-phenylvinyl acetate
-
-
4
2,2,2-trifluoroethyl acetate
-
-
0.82
2,2-difluoroethyl acetate
-
-
403
2,3-difluorophenyl acetate
-
-
334
2,4-difluorophenyl acetate
-
-
145
2,6-difluorophenyl acetate
-
-
0.5
2-fluoroethyl acetate
-
-
431
2-fluorophenyl acetate
-
-
0.66 - 55.2
2-naphthyl acetate
7
2-naphthyl butanoate
pH 7.0, 25
51
3,4-dimethyl phenyl acetate
-
-
125
3-cyanophenyl acetate
-
-
571
3-fluorophenyl acetate
-
-
99
3-naphthyl acetate
pH 7.0, 25
16.4
3-naphthyl butanoate
pH 7.0, 25
59
3-nitrophenyl acetate
-
-
14
4-acetoxy methyl benzoate
-
-
13
4-acetoxyacetophenone
-
pH 8.0
26
4-acetoxybenzaldehyde
117
4-chlorophenyl acetate
-
-
23
4-cyanophenyl acetate
-
-
0.244
4-diethyl phosphate methyl benzoate
-
pH 8.0
0.13 - 2500
4-nitrophenyl acetate
97.2
4-nitrophenyl butanoate
pH 7.0, 25
285
4-nitrophenyl butyrate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
341
4-nitrophenyl caprate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
467
4-nitrophenyl caproate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
410
4-nitrophenyl caprylate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
264
4-nitrophenyl laurate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
265
4-nitrophenyl palmitate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
269
4-nitrophenyl phosphate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
450
Alpha-naphthyl acetate
recombinant wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 60C
0.0008 - 0.02
Benzyl acetate
0.27
butyl acetate
-
-
0.242
ethyl acetate
-
-
18
naphthalen-2-yl acetate
-
pH 8.0, wild-type enzyme
68
p-tolyl acetate
-
-
0.35 - 4778
phenyl acetate
0.4
propyl acetate
-
-
20
S-methyl thiobutanoate
pH 8.0, 25
-
additional information
additional information
-
all kinetic parameters increase with temperature, but the ratios kcat/Km and Kp/Km remain practically constant
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1612
1-naphthyl acetate
in 50 mM Tris-HCl (pH 8.0), at 37C
6900
2-naphthyl acetate
pH 7.0, 25
540
2-naphthyl butanoate
pH 7.0, 25
19100
3-naphthyl acetate
pH 7.0, 25
1900
3-naphthyl butanoate
pH 7.0, 25
0.06 - 10600
4-nitrophenyl acetate
560
4-nitrophenyl butanoate
pH 7.0, 25
0.000133 - 23100
phenyl acetate
2.8
S-methyl thiobutanoate
pH 8.0, 25
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.508 - 0.598
diisopropylfluorophosphate
0.401 - 0.885
phenyl acetate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00014
-
partially purified enzyme, substrate diclofop methyl
0.00024
-
partially purified enzyme, substrate binapacryl
0.00031
-
partially purified enzyme, substrate binapacryl, population Rothamsted
0.00043
-
partially purified enzyme, substrate binapacryl, population Peldon
0.00049
-
partially purified enzyme, substrate binapacryl
0.00066
-
partially purified enzyme, substrate diclofop methyl
0.0048
-
partially purified enzyme, substrate bromoxynil octanoate, population Peldon
0.0056
-
partially purified enzyme, substrate bromoxynil octanoate
0.006
-
purified enzyme, substrate binapacryl
0.0061
-
partially purified enzyme, substrate bromoxynil octanoate, population Rothamsted
0.007
-
partially purified enzyme, substrate bromoxynil octanoate
0.03
-
partially purified enzyme, substrate 4-nitrophenyl acetate
0.04
-
partially purified enzyme, substrate 4-nitrophenyl acetate
0.0636
-
-
0.08
-
partially purified enzyme, substrate 4-nitrophenyl acetate
0.086
pH 8.0, 25C, substrate: phenyl acetate, purified recombinant enzyme
0.19
-
purified enzyme, substrate bromoxynil octanoate
0.7
pH 8.0, 25C, substrate: 4-nitrophenyl acetate, purified recombinant enzyme
0.96
pH 8.0, 25C, substrate: 4-nitrophenyl propionate, purified recombinant enzyme
1.22
-
isozyme 192Q, crude enzyme, using phenyl acetate as substrate, in 50 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 25C
1.3
pH 8.0, 25C, substrate: 4-nitrophenyl butyrate, purified recombinant enzyme
1.34
-
isozyme 192R, crude enzyme, using phenyl acetate as substrate, in 50 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 25C
1.4
pH 8.0, 25C, substrate: 4-nitrophenyl butyrate, purified recombinant enzyme
4.1
pH 8.0, 25C, substrate: phenyl acetate, purified recombinant enzyme
4.18
-
purified enzyme, substrate 4-nitrophenyl butyrate
5.15
-
recombinant enzyme from crude cell extract
6.5
recombinant enzyme from crude cell extract, using phenyl acetate as substrate, at 60C and pH 7.0
8.5
-
recombinant enzyme, after purification, in 20 mM Tris-HCl buffer (pH 7.4), at 37C
11.4
pH 8.0, 25C, substrate: 4-nitrophenyl butyrate, purified recombinant enzyme
13.6
pH 8.0, 25C, substrate: 4-nitrophenyl propionate, purified recombinant enzyme
20.7
pH 8.0, 25C, substrate: 4-nitrophenyl propionate, purified recombinant enzyme
35
-
recombinant Sm23, with 4-nitrophenyl acetate as substrate
39
pH 8.0, 25C, substrate: 4-nitrophenyl acetate, purified recombinant enzyme
40
-
-
77.9
-
purified enzyme, substrate 4-nitrophenyl acetate
85.05
-
using phenyl acetate as substrate (arylesterase activity of PON1), at 25C
155.6
-
37C, pH 7.6
159.2
-
purified enzyme, substrate beta-naphthyl acetate
170.7
-
purified enzyme, substrate alpha-naphthyl acetate
465
-
recombinant enzyme after 90fold purification
486 - 519
purified recombinant wild-type enzyme, substrate phenyl acetate
558
-
purified enzyme from phenotype A blood plasma
623
purified recombinant wild-type enzyme, substrate phenyl acetate in presence of 3.5% NaCl
882
-
purified enzyme from phenotype A blood plasma
970
-
isozyme 192Q, after 795fold purification, using phenyl acetate as substrate, in 50 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 25C
1048
-
isozyme 192R, after 783fold purification, using phenyl acetate as substrate, in 50 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 25C
1120
pH 8.0, 25C, substrate: phenyl acetate, purified recombinant enzyme
5580
recombinant enzyme after 858.5fold purification, using phenyl acetate as substrate, at 60C and pH 7.0
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6
-
; p-nitrophenyl acetate
7.8
-
p-nitrophenyl acetate
7.8 - 8
-
-
additional information
-
pH-rate profile of PON1 with 2-naphthyl acetate
pH RANGE