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The expected taxonomic range for this enzyme is: Mus musculus
Synonyms
3'-phosphoadenosine 5'-phosphosulfate-psychosine sulphotransferase, PAPS:psychosine sulphotransferase,
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3'-phosphoadenosine 5'-phosphosulfate-psychosine sulphotransferase
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PAPS:psychosine sulphotransferase
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3'-phosphoadenylyl sulfate + galactosylsphingosine = adenosine 3',5'-bisphosphate + psychosine sulfate
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sulfate group transfer
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3'-phosphoadenylyl-sulfate:galactosylsphingosine sulfotransferase
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3'-phosphoadenylylsulfate + galactosylsphingosine
adenosine 3',5'-bisphosphate + psychosine sulfate
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3'-phosphoadenylylsulfate + galactosylsphingosine
adenosine 3',5'-bisphosphate + psychosine sulfate
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Mg2+
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divalent ions other than magnesium causes a reduction of enzyme activity
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ATP
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decreases the incorporation of sulfate ions into psychosine sulfate
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EDTA
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stimulatory effect
glutathione
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stimulates SO42- incorporation by 23%
Tween 20
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detergent is necessary for enzyme action in vitro, removing Tween 20 from the incubation medium inhibits enzyme activity 92.5%
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0.5
galactosylsphingosine
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pH 7.4, 37°C, psychosine
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6.4 - 8
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about 50% of activity maximum at pH 6.4, about 60% of activity maximum at pH 7.4
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mouse, normal and Jimpy mutant, maximum enzyme activity at 17-19 days after birth
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additional information
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hardly any effect in liver
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45 - 55
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at 45°C enzyme activity is reduced by 85%, at 55°C activity is lost
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-20°C, microsomes or whole homogenate retains enzyme activity for at least 1 month
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Nussbaum, J.L.; Mandel, P.
Enzymic synthesis of psychosine sulphate
J. Neurochem.
19
1789-1802
1972
Mus musculus
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