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AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
ATP + 2-ketobutyrate + H2O
?
-
8% of the activity with pyruvate
-
-
?
ATP + 2-oxoglutarate + H2O
?
-
2% of the activity with pyruvate
-
-
?
ATP + 3-fluoropyruvate + H2O
AMP + (Z)-fluorophosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
additional information
?
-
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
-
-
-
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
the enzyme is involved in the production of pyruvate, phosphoenolpyruvate and ATP from glycerol in Pseudomonas fluorescens
-
-
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
-
-
-
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
the enzyme is involved in the production of pyruvate, phosphoenolpyruvate and ATP from glycerol in Pseudomonas fluorescens
-
-
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
-
-
-
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
-
-
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
CMP, GMP, IMP, UMP, CDP, GDP, IDP, and UDP are inert as the acceptor. Diphosphate can not be substituted for phosphate. The enzyme does not catalyze the diphosphate-dependent formation of pyruvate from phosphoenolpyruvate and AMP
-
-
r
ATP + 3-fluoropyruvate + H2O
AMP + (Z)-fluorophosphoenolpyruvate + phosphate
stereochemically specific reaction
-
r
ATP + 3-fluoropyruvate + H2O
AMP + (Z)-fluorophosphoenolpyruvate + phosphate
10times slower reaction rate than with pyruvate
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
?
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
ir
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
-
ir
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
highly specific
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
phosphorylated enzyme as an intermediate
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
equilibrium lies far to the side of phosphoenolpyruvate formation
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
in the reverse reaction dAMP yields 1% of the rate obtained with AMP, 3'-AMP gives no reaction
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
enzyme is essential for gluconeogenesis in Escherichia coli and Salmonella typhimurium during the growth on pyruvate, lactate, alanine or serine, in certain circumstances the enzyme may also provide phosphoenolpyruvate under glycolytic conditions
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
involved in gluconeogenesis
-
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
essential step in gluconeogenesis if pyruvate or lactate is used as carbon source
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
phospho-transfer mechanism, overview
-
-
ir
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
highly specific
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
in the reverse reaction dAMP yields 1% of the rate obtained with AMP, 3'-AMP gives no reaction
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
enzyme is essential for gluconeogenesis in Escherichia coli and Salmonella typhimurium during the growth on pyruvate, lactate, alanine or serine, in certain circumstances the enzyme may also provide phosphoenolpyruvate under glycolytic conditions
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
?
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
?
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
absolutely specific for ATP, highly specific for pyruvate
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
phosphorylated enzyme as an intermediate
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
equilibrium lies far to the side of phosphoenolpyruvate formation
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
involved in gluconeogenesis
-
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
?
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
?
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
involved in gluconeogenesis
-
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
ir
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
ir
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
-
r
additional information
?
-
-
no activity with 2-keto-3-methylvalerate, 2-ketoisocaproate, 2-ketomalonate, phenylpyruvate, hydroxyphenylpyruvate, imidazolpyruvate, and indole-3-pyruvate
-
-
?
additional information
?
-
neither GTP, CTP, ITP nor UTP is active as phosphoryl donor
-
-
?
additional information
?
-
-
neither GTP, CTP, ITP nor UTP is active as phosphoryl donor
-
-
?
additional information
?
-
neither GTP, CTP, ITP nor UTP is active as phosphoryl donor
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
the enzyme is involved in the production of pyruvate, phosphoenolpyruvate and ATP from glycerol in Pseudomonas fluorescens
-
-
?
AMP + phosphoenolpyruvate + phosphate
ATP + pyruvate + H2O
-
the enzyme is involved in the production of pyruvate, phosphoenolpyruvate and ATP from glycerol in Pseudomonas fluorescens
-
-
?
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
ir
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
-
-
-
ir
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
enzyme is essential for gluconeogenesis in Escherichia coli and Salmonella typhimurium during the growth on pyruvate, lactate, alanine or serine, in certain circumstances the enzyme may also provide phosphoenolpyruvate under glycolytic conditions
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
involved in gluconeogenesis
-
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
essential step in gluconeogenesis if pyruvate or lactate is used as carbon source
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
enzyme is essential for gluconeogenesis in Escherichia coli and Salmonella typhimurium during the growth on pyruvate, lactate, alanine or serine, in certain circumstances the enzyme may also provide phosphoenolpyruvate under glycolytic conditions
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
involved in gluconeogenesis
-
-
r
ATP + pyruvate + H2O
AMP + phosphoenolpyruvate + phosphate
-
involved in gluconeogenesis
-
-
r
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Berman, K.M.; Cohn, M.
Phosphoenolpyruvate synthetase of Escherichia coli. Purification, some properties, and the role of divalent metal ions
J. Biol. Chem.
245
5309-5318
1970
Escherichia coli
brenda
Berman, K.M.; Cohn, M.
Phosphoenolpyruvate synthetase. Partial reactions studied with adenosine triphosphate analogues and the inorganic phosphate-H2 18O exchange reaction
J. Biol. Chem.
245
5319-5325
1970
Escherichia coli
brenda
Cooper, R.A.; Kornberg, H.L.
Net formation of phosphoenolpyruvate from pyruvate by Escherichia coli
Biochim. Biophys. Acta
104
618-620
1965
Escherichia coli
brenda
Cooper, R.A.; Kornberg, H.L.
Phosphorylated enzyme as an intermediate in the phosphoenolpyruvate synthase reaction
Biochem. J.
105
49c-50c
1967
Escherichia coli
-
brenda
Cooper, R.A.; Kornberg, H.L.
Phosphoenolpyruvate synthetase and pyruvate, phosphate dikinase
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
10
631-649
1974
Escherichia coli, Escherichia coli B / ATCC 11303
-
brenda
Chulavatnatol, M.; Atkinson, D.E.
Phosphoenolpyruvate synthetase from Escherichia coli. Effects of adenylate energy charge and modifier concentrations
J. Biol. Chem.
248
2712-2715
1973
Escherichia coli, Escherichia coli B / ATCC 11303
brenda
Niersbach, M.; Kreuzaler, F.; Geerse, R.H.; Postma, P.W.; Hirsch, H.J.
Cloning and nucleotide sequence of the Escherichia coli K-12 ppsA gene, encoding PEP synthase
Mol. Gen. Genet.
231
332-336
1992
Escherichia coli (P23538)
brenda
Narindrasorasak, S.; Bridger, W.A.
Phosphoenolypyruvate synthetase of Escherichia coli: molecular weight, subunit composition, and identification of phosphohistidine in phosphoenzyme intermediate
J. Biol. Chem.
252
3121-3127
1977
Escherichia coli
brenda
Jakeman, D.L.; Evans, J.N.S.
Overexpression, purification, and use of phosphoenol pyruvate synthetase in the synthesis of PEP analogs
Bioorg. Chem.
26
245-253
1998
Escherichia coli (P23538)
-
brenda
Harauz, G.
Symmetry in the 2.25 MDa homomultimeric phosphoenolpyruvate synthase from Staphylothermus marinus: analyses of negatively stained preparations
Micron
29
161-173
1998
Staphylothermus marinus
-
brenda
Hutchins, A.M.; Holden, J.F.; Adams, M.W.
Phosphoenolpyruvate synthetase from the hyperthermophilic archaeon Pyrococcus furiosus
J. Bacteriol.
183
709-715
2001
Pyrococcus furiosus
brenda
Padilla, L.; Agosin, E.
Heterologous expression of Escherichia coli ppsA (phosphoenolpyruvate synthetase) and galU (UDP-glucose pyrophosphorylase) genes in Corynebacterium glutamicum, and its impact on trehalose synthesis
Metab. Eng.
7
260-268
2005
Escherichia coli (P23538), Escherichia coli
brenda
Tjaden, B.; Plagens, A.; Doerr, C.; Siebers, B.; Hensel, R.
Phosphoenolpyruvate synthetase and pyruvate, phosphate dikinase of Thermoproteus tenax: key pieces in the puzzle of archaeal carbohydrate metabolism
Mol. Microbiol.
60
287-298
2006
Thermoproteus tenax (Q70WQ8), Thermoproteus tenax, Thermoproteus tenax Kra1 (Q70WQ8)
brenda
Imanaka, H.; Yamatsu, A.; Fukui, T.; Atomi, H.; Imanaka, T.
Phosphoenolpyruvate synthase plays an essential role for glycolysis in the modified Embden-Meyerhof pathway in Thermococcus kodakarensis
Mol. Microbiol.
61
898-909
2006
Thermococcus kodakarensis, Thermococcus kodakarensis KW128
brenda
Burnell, J.N.
Cloning and characterization of Escherichia coli DUF299: a bifunctional ADP-dependent kinase-Pi-dependent pyrophosphorylase from bacteria
BMC Biochem.
11
1-1
2010
Zea mays
brenda
Auger, C.; Appanna, V.; Castonguay, Z.; Han, S.; Appanna, V.D.
A facile electrophoretic technique to monitor phosphoenolpyruvate-dependent kinases
Electrophoresis
33
1095-1101
2012
Pseudomonas fluorescens, Pseudomonas fluorescens ATCC 13525
brenda
Sakuraba, H.; Utsumi, E.; Schreier, H.J.; Ohshima, T.
Transcriptional regulation of phosphoenolpyruvate synthase by maltose in the hyperthermophilic archaeon, Pyrococcus furiosus
J. Biosci. Bioeng.
92
108-113
2001
Pyrococcus furiosus (P42850), Pyrococcus furiosus
brenda
Haferkamp, P.
Biochemical studies of enzymes involved in glycolysis of the thermoacidophilic crenarchaeon Sulfolobus solfataricus
PH. D. Thesis Universität Duisburg-Essen
2011
0000
2011
Saccharolobus solfataricus (Q97V40), Saccharolobus solfataricus DSM 1617 (Q97V40)
-
brenda
McCormick, N.E.; Jakeman, D.L.
On the mechanism of phosphoenolpyruvate synthetase (PEPs) and its inhibition by sodium fluoride: potential magnesium and aluminum fluoride complexes of phosphoryl transfer
Biochem. Cell Biol.
93
236-240
2015
Escherichia coli (P23538), Escherichia coli
brenda
Chen, X.; Li, M.; Zhou, L.; Shen, W.; Algasan, G.; Fan, Y.; Wang, Z.
Metabolic engineering of Escherichia coli for improving shikimate synthesis from glucose
Biores. Technol.
166
64-71
2014
Escherichia coli (P23538), Escherichia coli
brenda
Theriot, C.; Tove, S.; Grunden, A.
Characterization of two proline dipeptidases (prolidases) from the hyperthermophilic archaeon Pyrococcus horikoshii
Appl. Microbiol. Biotechnol.
86
177-188
2010
Pyrococcus horikoshii
brenda
Schäfer, T.; Schönheit, P.
Gluconeogenesis from pyruvate in the hyperthermophilic archaeon Pyrococcus furiosus involvement of reactions of the Embden-Meyerhof pathway
Arch. Microbiol.
159
354-363
1993
Pyrococcus furiosus (P42850)
-
brenda
Adams, M.; Holden, J.; Menon, A.; Schut, G.; Grunden, A.; Hou, C.; Hutchins, A.; Jenney F.E., J.; Kim, C.; Ma, K.; Pan, G.; Roy, R.; Sapra, R.; Story, S.; Verhagen, M.
Key role for sulfur in peptide metabolism and in regulation of three hydrogenases in the hyperthermophilic archaeon Pyrococcus furiosus
J. Bacteriol.
183
716-724
2001
Pyrococcus furiosus (P42850)
brenda
Barbier, T.; Zuniga-Ripa, A.; Moussa, S.; Plovier, H.; Sternon, J.F.; Lazaro-Anton, L.; Conde-Alvarez, R.; De Bolle, X.; Iriarte, M.; Moriyon, I.; Letesson, J.J.
Brucella central carbon metabolism an update
Crit. Rev. Microbiol.
44
182-211
2018
no activity in Brucella sp.
brenda
Alhasawi, A.; Thomas, S.C.; Appanna, V.D.
Metabolic networks to generate pyruvate, PEP and ATP from glycerol in Pseudomonas fluorescens
Enzyme Microb. Technol.
85
51-56
2016
Pseudomonas fluorescens, Pseudomonas fluorescens 13525
brenda