Information on EC 2.7.8.8 - CDP-diacylglycerol-serine O-phosphatidyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.8.8
-
RECOMMENDED NAME
GeneOntology No.
CDP-diacylglycerol-serine O-phosphatidyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
substituted phospho group transfer
-
-
-
-
transphosphatidylation
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
Glycerophospholipid metabolism
-
-
Glycine, serine and threonine metabolism
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-
Metabolic pathways
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phosphatidylethanolamine biosynthesis I
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superpathway of phospholipid biosynthesis II (plants)
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lipid metabolism
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phosphatidylethanolamine bioynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
CDP-diacylglycerol:L-serine 3-sn-phosphatidyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9068-48-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
cultivar japonica
-
-
Manually annotated by BRENDA team
cv. Kitaake
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
show the reaction diagram
-
41% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
-
-
?
CDP-1,2-diacylglycerol + L-serine
CMP + 1,2-diacyl-sn-glycerol-3-phospho-L-serine
show the reaction diagram
-
fatty acids from lecithin
-
-
?
CDP-1,2-dicaproyl-DL-glycerol + L-Ser
CMP + 3-O-sn-1,2-dicaproylphosphatidylserine
show the reaction diagram
-
-
-
-
?
CDP-1,2-dipalmitoyl-L-glycerol + L-Ser
CMP + 1,2-dipalmitoylphosphatidylserine
show the reaction diagram
-
-
-
-
?
CDP-2,3-bis-O-(oleoyl)-sn-glycerol + L-serine
CMP + 2,3-bis-O-(oleoyl)-sn-glycero-1-phospho-L-serine
show the reaction diagram
-
17% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
-
-
?
CDP-diacylglycerol + glycerol
CMP + phosphatidylglycerol
show the reaction diagram
-
low activity
-
?
CDP-diacylglycerol + H2O
CMP + phosphatidic acid
show the reaction diagram
CDP-diacylglycerol + L-Ser
CMP + 3-O-sn-phosphatidyl-L-serine
show the reaction diagram
CDP-diacylglycerol + L-serine
CMP + (3-sn-phosphatidyl)-L-serine
show the reaction diagram
CDP-diacylglycerol + L-serine
CMP + 3-O-sn-phosphatidyl-L-serine
show the reaction diagram
CDP-diacylglycerol + sn-glycero-3-phosphate
CMP + phosphatidylglycerophosphate
show the reaction diagram
phosphatidylserine + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CDP-diacylglycerol + L-serine
CMP + (3-sn-phosphatidyl)-L-serine
show the reaction diagram
CDP-diacylglycerol + L-serine
CMP + 3-O-sn-phosphatidyl-L-serine
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
dependent on
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
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inhibits activity as the molar ratio of Triton X-100 to CDP-diacylglycerol raises beyond the point of maximal activity
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cardiolipin
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activates. The enzyme is completely desensitized by treatment for 5 min at 40C against the effect of cadiolipin without loss of activity
diphosphatidylglycerol
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membrane association and activity of PtdSer synthase is increased, studied with mixed micelles containing phosphatidylglycerol (one charge) or diphosphatidylglycerol (two charges), the two main anionic membrane lipids in Escherichia coli
phosphatidylethanolamine
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slightly activates. The enzyme is completely desensitized by treatment for 5 min at 40C against the effect of phosphatidylethanolamine without loss of activity
phosphatidylglycerol
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membrane association and activity of PtdSer synthase is increased, studied with mixed micelles containing phosphatidylglycerol (one charge) or diphosphatidylglycerol (two charges), the two main anionic membrane lipids in Escherichia coli
Triton X-100
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.085
L-Ser
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pH 7.2, 37C, membrane-bound enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.34
extract supernatant
2.62
after ammonium sulfate precipitation
7.86
purification step SP-Sepharose HP
13.62
purification step Sephadex G-75
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.5
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-
7
transphosphatidylation
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
activity assay
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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isoform PSS1 promoter activity is prominent in developing anthers
Manually annotated by BRENDA team
isozyme OsPSS-1 gene is expressed predominantly in elongating cells
Manually annotated by BRENDA team
additional information
quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses of wild-type plants reveals that OsPSS-1 is expressed in various organs, including roots, culms, and leaves, with the highest expression in panicles
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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possible regulatory mechanism: cross-feedback regulatory model which assumes two forms of phosphatidylserine synthase, only molecules bound with acidic phospholipids of the membrane are active in phosphatidylserine synthesis, whereas others in the cytoplasm are latent
Manually annotated by BRENDA team
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isoform PSS1 is localized in endoplasmic reticulum membrane during pollen development
Manually annotated by BRENDA team
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isoform PSS1 is localized in nuclei during pollen development
Manually annotated by BRENDA team
additional information
OsPSS-1 localizes to organelles associated with exocytosis
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Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
-
x * 37000, SDS-PAGE
52800
theoretical
52817
-
x * 52817, calculation from nucleotide sequence
53000
determined by SDS-PAGE
54000
-
x * 54000, SDS-PAGE
55500
-
x * 55500, calculated from amino acid sequence
500000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
high ionic strength buffers are neceassary to prevent irreversible precipitation and inactivation of the enzyme
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, stable for ar least 1 month
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4C, 25% loss after 1 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
after ammonium sulfate precipitation on a SP-Sepharose and a Sephadex G-75 gel column
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli M15 (pREP4) cells
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expressed in HEK-293 cell
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into the vector pBES for expression in Bacillus subtilis DB104
transformation of OsPSS-1-GFP in Arabidopsis thaliana protoplasts, subcellular localization in endoplasmic reticulum, plasma membrane, and trans-Golgi network/early endosomes
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
food industry
phospholipids, especially phosphatidylserine, have many applications in functional food and pharmaceutical industries
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