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CDP-diacylglycerol + choline
CMP + phosphatidylcholine
additional information
?
-
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
r
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
phosphatidylcholine formation by enzyme Pcs is reversible and proceeds via two successive reactions
-
-
r
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
r
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
phosphatidylcholine formation by enzyme Pcs is reversible and proceeds via two successive reactions
-
-
r
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
r
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
the enzyme utilizes exogenous choline for phosphatidylcholine synthesis
-
-
r
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
phosphatidylcholine synthesis important for functionality of Dot/Icm translocator (formation of replicative vacuole), binding to macrophages, sufficient level of flagellin protein (linked to bacterial cytotoxicity, cellular adhesion)
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
overexpression of PcsA complements low phosphatidylcholine levels in membrane of mutant strain lacking PcsA or lacking both PcsA and PmtA (phospholipid N-methyltransferase)
lipid analysis by thin-layer chromatography
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
the phosphatidylcholine synthase gene is solely responsible for the ability of the organism to synthesize phosphatidylcholine
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
second bacterial pathway for phosphatidylcholine biosynthesis
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
second bacterial pathway for phosphatidylcholine biosynthesis
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
additional information
?
-
no activity with gamma-butyrobetaine, betaine, and carnitine instead of choline
-
-
?
additional information
?
-
-
no activity with gamma-butyrobetaine, betaine, and carnitine instead of choline
-
-
?
additional information
?
-
the enzyme exhibits broad substrate specificity towards choline derivatives. The presence of CDP-diacylglycerol and manganese is a prerequisite for cooperative binding of choline. No activity with gamma-butyrobetaine, betaine, and carnitine instead of choline
-
-
?
additional information
?
-
-
the enzyme exhibits broad substrate specificity towards choline derivatives. The presence of CDP-diacylglycerol and manganese is a prerequisite for cooperative binding of choline. No activity with gamma-butyrobetaine, betaine, and carnitine instead of choline
-
-
?
additional information
?
-
no activity with gamma-butyrobetaine, betaine, and carnitine instead of choline
-
-
?
additional information
?
-
the enzyme exhibits broad substrate specificity towards choline derivatives. The presence of CDP-diacylglycerol and manganese is a prerequisite for cooperative binding of choline. No activity with gamma-butyrobetaine, betaine, and carnitine instead of choline
-
-
?
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CDP-diacylglycerol + choline
CMP + phosphatidylcholine
additional information
?
-
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
r
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
-
-
r
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
the enzyme utilizes exogenous choline for phosphatidylcholine synthesis
-
-
r
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
phosphatidylcholine synthesis important for functionality of Dot/Icm translocator (formation of replicative vacuole), binding to macrophages, sufficient level of flagellin protein (linked to bacterial cytotoxicity, cellular adhesion)
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
the phosphatidylcholine synthase gene is solely responsible for the ability of the organism to synthesize phosphatidylcholine
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
second bacterial pathway for phosphatidylcholine biosynthesis
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
second bacterial pathway for phosphatidylcholine biosynthesis
-
?
CDP-diacylglycerol + choline
CMP + phosphatidylcholine
-
enzyme of the phosphatidylcholine synthase pathway
-
-
?
additional information
?
-
no activity with gamma-butyrobetaine, betaine, and carnitine instead of choline
-
-
?
additional information
?
-
-
no activity with gamma-butyrobetaine, betaine, and carnitine instead of choline
-
-
?
additional information
?
-
no activity with gamma-butyrobetaine, betaine, and carnitine instead of choline
-
-
?
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Mg2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+
Mg2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+
Mg2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+
Mg2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+
Mg2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+
Mg2+
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+
Mg2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+
Mg2+
-
20fold less effective than Mn2+
Mg2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+
Mn2+
required for activity
Mn2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+. At 10 mM Mn2+ maximal activity is reached
Mn2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+. At 10 mM Mn2+ maximal activity is reached
Mn2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+. At 10 mM Mn2+ maximal activity is reached
Mn2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+. At 10 mM Mn2+ maximal activity is reached
Mn2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+. At 10 mM Mn2+ maximal activity is reached
Mn2+
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+. At 10 mM Mn2+ maximal activity is reached
Mn2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+. At 10 mM Mn2+ maximal activity is reached
Mn2+
-
optimal activation at 10 mM, more effective than Mg2+
Mn2+
-
requires the presence of bivalent cations such as Mg2+ or Mn2+ for its function, with Mn2+ being much more efficient (more than 20fold higher activity) than Mg2+. At 10 mM Mn2+ maximal activity is reached
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malfunction
-
a deletion mutant is much more sensitive than the wild type strain when challenged with Al3+ and tetradecyltrimethylammonium bromide, and shows a significant loss of viability
malfunction
-
a deletion mutant is much more sensitive than the wild type strain when challenged with Al3+ and tetradecyltrimethylammonium bromide, and shows a significant loss of viability
-
metabolism
-
the enzyme is responsible for the synthesis of phosphatidylcholine, which acts as a temporary reservoir for Al3+
metabolism
the phosphatidylcholine synthase pathway is the primary pathway for synthesis of phosphatidylcholine in Legionelly dumoffii. Choline is condensed directly with CDP-diacylglyceride to form phosphatidylcholine in a reaction catalyzed by a bacterium-specific Pcs enzyme
metabolism
-
the enzyme is responsible for the synthesis of phosphatidylcholine, which acts as a temporary reservoir for Al3+
-
physiological function
phosphatidylcholine, produced by the enzyme, is essential for the expression of virulence-related genes that is controlled by the two-component system VirA/G
physiological function
the enzyme synthesizes phosphatidylcholine, a membrane-forming phospholipid, via the phosphatidylcholine synthase pathway, Pcs. Structurally different PC species are distributed in the outer and inner membranes
physiological function
-
phosphatidylcholine, produced by the enzyme, is essential for the expression of virulence-related genes that is controlled by the two-component system VirA/G
-
additional information
cytoplasmic loop between the second and third membrane helix contains the majority of the conserved amino acids of a CDP-alcohol phosphotransferase motif (DGX2ARX12GX3DX3D), crucial role of this motif in choline binding and enzyme activity
additional information
-
cytoplasmic loop between the second and third membrane helix contains the majority of the conserved amino acids of a CDP-alcohol phosphotransferase motif (DGX2ARX12GX3DX3D), crucial role of this motif in choline binding and enzyme activity
additional information
-
cytoplasmic loop between the second and third membrane helix contains the majority of the conserved amino acids of a CDP-alcohol phosphotransferase motif (DGX2ARX12GX3DX3D), crucial role of this motif in choline binding and enzyme activity
-
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De Rudder, K.E.E.; Sohlenkamp, C.; Geiger, O.
Plant-exuded choline is used for rhizobial membrane lipid biosynthesis by phosphatidylcholine synthase
J. Biol. Chem.
274
20011-20016
1999
Sinorhizobium meliloti
brenda
Sohlenkamp, C.; de Rudder, K.E.E.; Rhrs, V.; Lopez-Lara, I.M.; Geiger, O.
Cloning and characterization of the gene for phosphatidylcholine synthase
J. Biol. Chem.
275
18919-18925
2000
Sinorhizobium meliloti (Q9KJY8), Sinorhizobium meliloti
brenda
Wilderman, P.J.; Vasil, A.I.; Martin, W.E.; Murphy, R.C.; Vasil, M.L.
Pseudomonas aeruginosa synthesizes phosphatidylcholine by use of the phosphatidylcholine synthase pathway
J. Bacteriol.
184
4792-4799
2002
Pseudomonas aeruginosa
brenda
Martinez-Morales, F.; Schobert, M.; Lopez-Lara, I.M.; Geiger, O.
Pathways for phosphatidylcholine biosynthesis in bacteria
Microbiology
149
3461-3471
2003
Agrobacterium tumefaciens, Borreliella burgdorferi, Bradyrhizobium japonicum, Brucella melitensis (D0B707), Brucella melitensis, Legionella pneumophila, Mesorhizobium loti, Pseudomonas aeruginosa, Rhizobium leguminosarum, Sinorhizobium meliloti
brenda
Wang, X.G.; Scagliotti, J.P.; Hu, L.T.
Phospholipid synthesis in Borrelia burgdorferi: BB0249 and BB0721 encode functional phosphatidylcholine synthase and phosphatidylglycerolphosphate synthase proteins
Microbiology
150
391-397
2004
Borreliella burgdorferi (O51265), Borreliella burgdorferi
brenda
Wessel, M.; Kluesener, S.; Goedeke, J.; Fritz, C.; Hacker, S.; Narberhaus, F.
Virulence of Agrobacterium tumefaciens requires phosphatidylcholine in the bacterial membrane
Mol. Microbiol.
62
906-915
2006
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
brenda
Conover, G.M.; Martinez-Morales, F.; Heidtman, M.I.; Luo, Z.Q.; Tang, M.; Chen, C.; Geiger, O.; Isberg, R.R.
Phosphatidylcholine synthesis is required for optimal function of Legionella pneumophila virulence determinants
Cell. Microbiol.
10
514-528
2008
Legionella pneumophila
brenda
Solis-Oviedo, R.L.; Martinez-Morales, F.; Geiger, O.; Sohlenkamp, C.
Functional and topological analysis of phosphatidylcholine synthase from Sinorhizobium meliloti
Biochim. Biophys. Acta
1821
573-581
2012
Sinorhizobium meliloti (Q9KJY8), Sinorhizobium meliloti
brenda
Geiger, O.; Lopez-Lara, I.M.; Sohlenkamp, C.
Phosphatidylcholine biosynthesis and function in bacteria
Biochim. Biophys. Acta
1831
503-513
2013
Agrobacterium tumefaciens, Bacillus subtilis, Brucella abortus, Brucella melitensis, Escherichia coli, Pseudomonas aeruginosa (Q9HXE9), Pseudomonas putida, Pseudomonas putida KT 2240, Sinorhizobium meliloti
brenda
He, H.; Wu, B.; Xiong, M.; Li, Y.; Wu, W.; Wang, X.
Gene cloning, structural gene and promoter identification, and active assay of the phosphatidylcholine synthase of Pseudomonas sp. strain 593
Can. J. Microbiol.
57
785-794
2011
Pseudomonas sp. (E9NR86), Pseudomonas sp., Pseudomonas sp. 593 (E9NR86)
brenda
Palusinska-Szysz, M.; Janczarek, M.; Kalitynski, R.; Dawidowicz, A.L.; Russa, R.
Legionella bozemanae synthesizes phosphatidylcholine from exogenous choline
Microbiol. Res.
166
87-98
2011
Legionella bozemanae (D5KX81), Legionella bozemanae, Legionella bozemanae ATCC 33217 (D5KX81)
brenda
Boeris, P.S.; Lucchesi, G.I.
The phosphatidylcholine synthase of Pseudomonas putida A ATCC 12633 is responsible for the synthesis of phosphatidylcholine, which acts as a temporary reservoir for Al3+
Microbiology
158
1249-1257
2012
Pseudomonas putida, Pseudomonas putida A ATCC 12633
brenda
Aktas, M.; Koester, S.; Kizilirmak, S.; Casanova, J.C.; Betz, H.; Fritz, C.; Moser, R.; Yildiz, O.e.; Narberhaus, F.
Enzymatic properties and substrate specificity of a bacterial phosphatidylcholine synthase
FEBS J.
281
3523-3541
2014
Agrobacterium tumefaciens (A9CIM3), Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970 (A9CIM3)
brenda
Palusinska-Szysz, M.; Szuster-Ciesielska, A.; Kania, M.; Janczarek, M.; Chmiel, E.; Danikiewicz, W.
Legionella dumoffii utilizes exogenous choline for phosphatidylcholine synthesis
Int. J. Mol. Sci.
15
8256-8279
2014
Fluoribacter dumoffii (S4X5L9), Fluoribacter dumoffii
brenda