Information on EC 2.7.8.13 - phospho-N-acetylmuramoyl-pentapeptide-transferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.8.13
-
RECOMMENDED NAME
GeneOntology No.
phospho-N-acetylmuramoyl-pentapeptide-transferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
substituted phospho group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Peptidoglycan biosynthesis
-
-
peptidoglycan biosynthesis I (meso-diaminopimelate containing)
-
-
peptidoglycan biosynthesis II (staphylococci)
-
-
peptidoglycan biosynthesis IV (Enterococcus faecium)
-
-
peptidoglycan biosynthesis V (beta-lactam resistance)
-
-
peptidoglycan biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-MurAc(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala):undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide-transferase
In Gram-negative and some Gram-positive organisms the L-lysine is replaced by meso-2,6-diaminoheptanedioate (meso-2,6-diaminopimelate, A2pm), which is combined with adjacent residues through its L-centre. The undecaprenol involved is ditrans,octacis-undecaprenol (for definitions, click here).
CAS REGISTRY NUMBER
COMMENTARY hide
9068-50-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain KM
-
-
Manually annotated by BRENDA team
strain KM
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain DH5alpha
-
-
Manually annotated by BRENDA team
strain K12
-
-
Manually annotated by BRENDA team
strain LMC500
-
-
Manually annotated by BRENDA team
strain Y-10
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
recombinant
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-dansyl-UDP-NAc-muramoyl-L-Ala-gamma-D-Glu-meso-diaminopimelic acid-D-Ala-D-Ala + undecaprenyl phosphate
UMP + ?
show the reaction diagram
N-fluorescamine-UDP-NAc-muramoyl-L-Ala-gamma-D-Glu-meso-diaminopimelic acid-D-Ala-D-Ala + undecaprenyl phosphate
UMP + ?
show the reaction diagram
N-o-phthalaldehyde-UDP-NAc-muramoyl-L-Ala-gamma-D-Glu-meso-diaminopimelic acid-D-Ala-D-Ala + undecaprenyl phosphate
UMP + ?
show the reaction diagram
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
show the reaction diagram
UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
show the reaction diagram
UDP-MurNAc + undecaprenyl phosphate
UMP + MurNAc-diphosphoundecaprenol
show the reaction diagram
UDP-MurNAc(oyl-L-Ala-gamma-D-Glu-meso-2,6-diaminopimelyl-(N-dansyl)-D-Ala-D-Ala) + dodecaprenyl phosphate
UMP + MurNAc(oyl-L-Ala-gamma-D-Glu-meso-2,6-diaminopimelyl-(N-dansyl)-D-Ala-D-Ala)-diphosphododecaprenol
show the reaction diagram
-
dPP
-
r
UDP-MurNAc-(oyl-L-Ala) + undecaprenyl phosphate
UMP + MurNAc(oyl-L-Ala)-diphosphoundecaprenol
show the reaction diagram
UDP-MurNAc-(oyl-L-Ala-gamma-D-Glu) + undecaprenyl phosphate
UMP + MurNAc(oyl-L-Ala-gamma-D-Glu)-diphosphoundecaprenol
show the reaction diagram
-
36% activity compared to UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)
-
-
r
UDP-MurNAc-(oyl-L-Ala-gamma-D-Glu-meso-diaminopimelate) + undecaprenyl phosphate
UMP + MurNAc(oyl-L-Ala-gamma-D-Glu-meso-diaminopimelate)-diphosphoundecaprenol
show the reaction diagram
-
51% activity compared to UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)
-
-
r
UDP-MurNAc-L-Ala-gamma-D-Glu-m-DAP-[Nepsilon-dansyl]-D-Ala-D-Ala + undecaprenyl phosphate
UMP + MurNAc-L-Ala-gamma-D-Glu-m-DAP-[Nepsilon-dansyl]-D-Ala-D-Ala-diphosphoundecaprenol
show the reaction diagram
UDP-MurNAc-pentapeptide + C55-isoprenyl phosphate
C55-isoprenyl-diphosphate-MurNAc-pentapeptide
show the reaction diagram
UDP-MurNAc-pentapeptide + undecaprenoid-1-ol-phosphate
lipid-P-P-MurNAc-pentapeptide + UMP
show the reaction diagram
UDP-MurNAc-pentapeptide + undecaprenyl phosphate
UMP + MurNAc-pentapeptide-diphosphoundecaprenol
show the reaction diagram
UDP-MurNAc-pentapeptide + undecaprenyl phosphate
UMP + undecaprenol diphosphate N-acetylmuramyl pentapeptide
show the reaction diagram
-
30 min, 35°C, pH 7.5
-
-
?
UDP-MurNAc-pentapeptide + undecaprenyl-phosphate
UMP + MurNAc-pentapeptide diphosphoryl undecaprenol
show the reaction diagram
UDP-N-acetyl-alpha-D-glucosamine + ditrans,octacis-undecaprenyl phosphate
UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
show the reaction diagram
UDP-N-acetylmuramoyl-pentapeptide + undecaprenyl phosphate
?
show the reaction diagram
-
a homogeneous fluorescence resonance energy transfer (FRET)-based assay for the translocase activity of MraY in which the donor fluorophore is attached to UNAM-pp and the acceptor is embedded in detergent micelles containing MraY and undecaprenyl phosphate is described
-
-
?
UDP-N-acetylmuramoyl-pentapeptide + undecaprenyl phosphate
UMP + N-acetylmuramoyl-pentapeptide-diphosphoundecaprenol
show the reaction diagram
25°C, pH 7.2
lipid I synthesis, peptidoglycan biosynthesis
-
?
UDP-N-acetylmuramoyl-pentapeptide + [2H]UMP
UMP + [2H]UDP-NAc-muramoyl-pentapeptide
show the reaction diagram
UDP-NAc-muramoyl-L-Ala-gamma-D-Glu-meso-diaminopimelic acid-D-Ala-D-Ala + undecaprenyl phosphate
UMP + ?
show the reaction diagram
UDP-NAc-muramoyl-pentapeptide + acceptor
UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
show the reaction diagram
UDP-NAc-muramoyl-pentapeptide + acceptor
UMP + acceptor-phospho-NAc-muramoyl-pentapeptide +
show the reaction diagram
UDP-NAc-muramoyl-pentapeptide + dodecaprenyl-phosphate
UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
show the reaction diagram
-
dodecaprenyl phosphate is a more efficient substrate than heptaprenyl-phosphate, enzyme is selective for larger substrate which is closer in chain length to the natural substrate, undecaprenyl-phosphate
-
r
UDP-NAc-muramoyl-pentapeptide + heptaprenyl-phosphate
UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
show the reaction diagram
-
-
-
r
UDP-NAc-muramoyl-pentapeptide + undecaprenyl phosphate
UMP + undecaprenyl-phospho-NAc-muramoyl-pentapeptide
show the reaction diagram
UDPMur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
show the reaction diagram
UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
show the reaction diagram
UDP-MurNAc-pentapeptide + undecaprenyl phosphate
UMP + MurNAc-pentapeptide-diphosphoundecaprenol
show the reaction diagram
UDP-MurNAc-pentapeptide + undecaprenyl-phosphate
UMP + MurNAc-pentapeptide diphosphoryl undecaprenol
show the reaction diagram
UDP-N-acetyl-alpha-D-glucosamine + ditrans,octacis-undecaprenyl phosphate
UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
show the reaction diagram
UDP-NAc-muramoyl-pentapeptide + acceptor
UMP + acceptor-phospho-NAc-muramoyl-pentapeptide +
show the reaction diagram
UDPMur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
show the reaction diagram
-
catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis
-
-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5'-O-(5-amino-5-deoxy-beta-D-ribofuranosyl)-3'-deoxy-3',3'-difluorouridine
-
low inhibition (29% at 11.4 mM), analogue of a liposidomycin (potent and selective inhibitor) assembled from lactol and gem-difluoromethylenated nucleoside with 1,2-trans-beta-furanoside linkage
A-94964
Amphomycin
-
-
caprazamycin B
-
-
CPZEN-45
-
CPZEN-45 strongly inhibits incorporation of radiolabeled glycerol into growing cultures and shows antibacterial activity against caprazamycin-resistant strains, including a strain overexpressing translocase-I MraY, involved in the biosynthesis of peptidoglycan, the target of the caprazamycins. Very poor inhibition by vancomycin
dodecylamine
-
a competitive inhibitor of MraY
EDTA
-
complete inhibition at 2 mM
liposidomycin C
-
specific inhibitor of peptidoglycan synthesis of bacteria
mureidomycin A
mureidomycin B
-
IC50: 0.000038 mM
N-(uracilylpentyl)-beta-D-O-(5-amino-5-deoxyribosyl)-L-serine
-
synthesis of analogue of naturally occurring inhibitors like liposidomycins and caprazamycins in which uridine moiety is replaced by a C5 acyclic alkyl chain, and uracil, aminoribose and amino acid moieties are retained
protein E
-
Ristomycin
-
-
RU66950
-
IC50: 0.07 mM
-
RU70157
-
IC50: 0.0054 mM
-
RU72143
-
IC50: 0.0015 mM
-
RU72839
-
IC50: 0.0003 mM
-
RU74709
-
IC50: 0.000096 mM
-
RU74854
-
IC50: 0.000022 mM
-
RU75413
-
IC50: 0.000033 mM
-
RU76491
-
IC50: 0.000084 mM
-
RU76492
-
IC50: 0.0001 mM
-
RU77254
-
IC50: 0.000027 mM
-
RU78695
-
IC50: 0.000034 mM
-
RU80303
-
IC50: 0.0005 mM
-
RU88108
-
IC50: 0.000051 mM
-
RU88110
-
IC50: 0.000016 mM
-
RU88114
-
IC50: 0.000084 mM
-
RU88155
-
IC50: 0.0015 mM
-
Triton X-100
-
activity is lost upon addition, can be restored by addition of lipid fractions to the assay
tunicamycin
Vancomycin
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Moenomycin
-
activates partially purified enzyme, maximal activation at 1 mg moenomycin/mg protein
Neutral lipid
-
stimulates synthesis of C55-isoprenyl-P-P-MurNAc-pentapeptide from UDPMurNAc-pentapeptide, no effect on exchange reaction of UDP-MurNAc-pentapeptide with UMP
-
phosphatidylglycerol
-
activity is stimulated 5-10fold by inclusion of 0.1 mg/ml in both radiochemical and fluorescence enhancement assays
Phospholipid
-
phospholipide sensitive to phospholipase necessary for enzymatic activity
Polar lipid fraction
-
required by exchange reaction of UDP-MurNAc-pentapeptide with UMP
-
undecaprenyl phosphate
-
stimulates exchange reaction
Undecaprenyl-diphosphate-MurNAc-pentapeptide
-
stimulates exchange reaction
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.067 - 0.087
5'-UMP
0.019
dansyl-UDP-NAc-muramoyl-pentapeptide
-
pH 7.5, 30°C
-
0.013
dodecaprenylphosphate
-
pH 7.5, 30°C
0.019
heptaprenylphosphate
-
pH 7.5, 30°C
1
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)
-
-
0.083
UDP-MurNAc-Ala-D-Glu-D-Ala-Glu
-
pH 7.8, 37°C, transfer reaction
0.022
UDP-MurNAc-Ala-D-Glu-Dap-D-Ala-D-Ala
-
pH 7.8, 37°C, exchange reaction
0.18
UDP-MurNAc-Ala-D-Glu-Lys
-
pH 7.8, 37°C
0.058 - 0.4
UDP-MurNAc-Ala-D-Glu-Lys-D-Ala
0.02 - 0.055
UDP-MurNAc-Ala-D-Glu-Lys-D-Ala-D-Ala
0.029
UDP-MurNAc-Ala-D-Glu-Lys-D-Ala-Gly
-
pH 7.8, 37°C, exchange reaction
0.063 - 0.44
UDP-MurNAc-Ala-D-Glu-Lys-Gly-D-Ala
0.1
UDP-MurNAc-Ala-D-Glu-mDap-D-Ala-D-Ala
-
pH 7.8, 37°C, transfer reaction
0.016
UDP-MurNAc-Ala-D-Glu-Orn-D-Ala-D-Ala
-
pH 7.8, 37°C, exchange reaction
4.4
UDP-MurNAc-Ala-Glu-Lys
-
pH 7.8, 37°C, transfer reaction
0.044 - 0.5
UDP-MurNAc-Gly-D-Glu-Lys-D-Ala-D-Ala
0.087 - 0.27
UDP-MurNAc-pentapeptide
0.34 - 3.4
UDP-N-acetylmuramoyl-pentapeptide
0.0018 - 0.037
UDP-NAc-muramoyl-pentapeptide
0.027
UMP
-
pH 7.8, 25°C
0.15 - 0.35
undecaprenyl phosphate
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.33
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)
Bacillus subtilis
-
-
0.00005 - 5.3
UDP-N-acetylmuramoyl-pentapeptide
0.00005 - 5.33
undecaprenyl phosphate
additional information
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000036
mureidomycin A
-
pH 7.5, 30°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1
A-94964
0.00005
caprazamycin B
Bacillus subtilis
-
pH and temperature not specified in the publication
0.0004
CPZEN-45
Bacillus subtilis
-
pH and temperature not specified in the publication
1
dodecylamine
Bacillus subtilis
-
pH 7.6, 37°C
0.000038
mureidomycin B
Escherichia coli
-
IC50: 0.000038 mM
0.07
RU66950
Escherichia coli
-
IC50: 0.07 mM
-
0.0054
RU70157
Escherichia coli
-
IC50: 0.0054 mM
-
0.0015
RU72143
Escherichia coli
-
IC50: 0.0015 mM
-
0.0003
RU72839
Escherichia coli
-
IC50: 0.0003 mM
-
0.000096
RU74709
Escherichia coli
-
IC50: 0.000096 mM
-
0.000022
RU74854
Escherichia coli
-
IC50: 0.000022 mM
-
0.000033
RU75413
Escherichia coli
-
IC50: 0.000033 mM
-
0.000084
RU76491
Escherichia coli
-
IC50: 0.000084 mM
-
0.0001
RU76492
Escherichia coli
-
IC50: 0.0001 mM
-
0.000027
RU77254
Escherichia coli
-
IC50: 0.000027 mM
-
0.000034
RU78695
Escherichia coli
-
IC50: 0.000034 mM
-
0.0005
RU80303
Escherichia coli
-
IC50: 0.0005 mM
-
0.000051
RU88108
Escherichia coli
-
IC50: 0.000051 mM
-
0.000016
RU88110
Escherichia coli
-
IC50: 0.000016 mM
-
0.000084
RU88114
Escherichia coli
-
IC50: 0.000084 mM
-
0.0015
RU88155
Escherichia coli
-
IC50: 0.0015 mM
-
0.0019 - 0.012
tunicamycin
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00002
-
mutant H289R
0.00013
-
mutant D99N
0.00024
-
mutant D98N
0.000276
-
specific activity of particulate enzyme from recombinant strain JM109 (pBROC525), in absence of exogenous lipid acceptor
0.00078
-
mutant K116Q
0.0024
-
mutant H290R
0.0058
-
mutant N171A
0.0066
-
mutant D174N
0.0072
-
mutant D231N
0.0086
-
mutant H291R
0.0087
-
mutant K48Q
0.0092
-
mutant H45R
0.026
-
mutant N168A
0.028
-
mutant D177N
0.18
-
mutant E299Q
1.7
-
wild-type
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6
-
assay at
9.4
-
9.0-9.4, mutant D98N
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
-
activity is significantly weaker at values below pH7.5 or over pH 9.0, about 40% of activity maximum at pH 6.5, about 80% of activity maximum at pH 9.0
6.8 - 9.4
-
mutant D98N shows different pH profile (specific activity ratio between pH9.4/pH7.6: 2.5) than wild-type (and all mutants except mutant D98N, specific activity ratio between pH9.4/pH7.6: 0.22-0.83)
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.9
-
electrofocusing in a pH gradient 3-8 containing 8 M urea
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
[Clostridium] bolteae 90A9
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000 - 45000
-
SDS-PAGE
100000 - 200000
-
second peak of exchange activity, low molecular weight form, gel filtration
2000000
-
major peak of activity, high molecular weight form, gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
no loss of activity observed upon gel filtration, desalted enzyme is completely inactive
-
only 2.5% of the activity can be recovered after dialysis
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-196°C, retains complete activity for at least 2 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from membrane extracts in presence of DDM detergent and by Ni-NTA metal affinity chromatography
-
low molecular weight form
-
partially
-
solubilization from membrane preparation through shaking for 30 min at 4°C at pH 7.5 and in presence of 1 mM MgCl2 and 2 mM beta-mercaptoethanol
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
amplification of the mraY gene, previously called open reading frame Y (ORF-Y,1,080 bp) at 2 min in the chromosome map
-
cloning of the murE-dd1B region, the cell wall biosynthesis and cell division gene cluster, expressed in Escherichia coli BL21(DE3)
-
expression of wild-type enzyme and mutant enzymes D115N, D116N and D267N as C-terminal hexahistidine fusion proteins
-
gene mraY, cloning in Escherichia coli strain DH5alpha
-
His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha
in medium-copy plasmid pBAD30 for complementation studies in Escherichia coli K-12 mutant strain RY3321 lacking endogenous MraY
-
in pET28b for site-directed mutagenesis and expression with N-terminal hexa-His-tag in Escherichia coli C43(DE3) or functional complementation in BW25113-derived thermosensitive strain (EcoliTsMraY) lacking endogenous MraY
-
mraY gene cloned into pASK-IBA3 vector and overexpressed
-
overexpressed in recombinant Escherichia coli JM109
-
Staphylococcus aureus MraY is functional in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D174N
-
0.39% of wild-type activity, 70% of optimum activity in presence of 250 mM Mg2+, altered catalytic efficiency (kcat), not capable of functional complementation, invariant residue in third cytoplasmic segment
D177N
-
1.6% of wild-type activity, 70% of optimum activity in presence of 250 mM Mg2+, altered catalytic efficiency (kcat), invariant residue in third cytoplasmic segment
D98A
-
site-directed mutagenesis, the mutant shows altered pH dependence compared to the wild-type enzyme
D98N
-
0.014% of wild-type activity, shifted pH optimum (pH 9.0-9.4) compared to wild-type (pH 7.5), highly decreased turnover (kcat, pH 7.2, elevated turnover at pH 9.4), decreased KM for UDP-N-acetylmuramoyl-pentapeptide (pH 7.2 and pH 9.4), increased KM for undecaprenyl phosphate (2fold at pH 7.2, 6fold at pH 9.4), not capable of functional complementation, invariant residue in second cytoplasmic segment
D99N
-
0.0076% of wild-type activity, highly decreased catalytic efficiency (kcat), increased KM for undecaprenyl phosphate, not capable of functional complementation, invariant residue in second cytoplasmic segment
E299Q
-
11% of wild-type activity, catalytic activity similarly affected by the presence of 250 mM Mg2+ as wild-type, slightly altered catalytic efficiency (kcat, 10%), invariant residue in fifth cytoplasmic segment
H289R
-
0.0012% of wild-type activity, highly decreased catalytic efficiency (kcat), not capable of functional complementation, invariant residue in fifth cytoplasmic segment
H290R
-
0.14% of wild-type activity, altered catalytic efficiency (kcat), not capable of functional complementation, invariant residue in fifth cytoplasmic segment
H291R
-
0.50% of wild-type activity, altered catalytic efficiency (kcat), increased KM for UDP-N-acetylmuramoyl-pentapeptide, invariant residue in fifth cytoplasmic segment
H45R
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0.54% of wild-type activity, 70% of optimum activity in presence of 250 mM Mg2+, altered catalytic efficiency (kcat), increased KM for UDP-N-acetylmuramoyl-pentapeptide, invariant residue in first cytoplasmic segment
K116Q
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0.046% of wild-type activity, highly decreased catalytic efficiency (kcat), not capable of functional complementation, invariant residue in second cytoplasmic segment
K48Q
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0.51% of wild-type activity, altered catalytic efficiency (kcat), increased KM for UDP-N-acetylmuramoyl-pentapeptide, invariant residue in first cytoplasmic segment
N168A
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1.5% of wild-type activity, altered catalytic efficiency (kcat), invariant residue in third cytoplasmic segment
N171A
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0.34% of wild-type activity, altered catalytic efficiency (kcat), not capable of functional complementation, invariant residue in third cytoplasmic segment
D98A
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site-directed mutagenesis, the mutant shows altered pH dependence compared to the wild-type enzyme
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D115N
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mutatio eliminates translocase 1 activity
D116N
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mutatio eliminates translocase 1 activity
deltaL172
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single-codon deletion in putative transmembrane domain TMD5, mediates PhiX174-resistancy only in catalytically active form and at high culture densitiy
F288L
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missense mutation in putative transmembrane domain TMD9, mediates PhiX174-resistancy only in catalytically active form
G168S
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mediates PhiX174-resistancy only at high culture densitiy, similar to wild-type
P170L
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mediates PhiX174-resistancy only in catalytically active form and at high culture densitiy, interacts with Epos gene product more strongly than with protein E
V291M
-
mediates PhiX174-resistancy only at high culture densitiy, similar to wild-type
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
drug development
medicine
molecular biology
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synthesis of radiolabelled UDP-MurNAc-pentapeptide as biochemical tools for studying peptidoglycan biosynthesis or the kinetic characterization of MraY
synthesis
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