Information on EC 2.7.7.5 - sulfate adenylyltransferase (ADP)

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The expected taxonomic range for this enzyme is: Archaea, Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.7.5
-
RECOMMENDED NAME
GeneOntology No.
sulfate adenylyltransferase (ADP)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ADP + sulfate = phosphate + adenylyl sulfate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sulfite oxidation II
-
-
sulfate reduction
-
-
Sulfur metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
ADP:sulfate adenylyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9027-70-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
thermophilic, DSM 3772
-
-
Manually annotated by BRENDA team
Saccharomyces fragilis
-
-
-
Manually annotated by BRENDA team
strain RT
SwissProt
Manually annotated by BRENDA team
strain RT
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + phosphate
phosphate + ADP
show the reaction diagram
CDP + phosphate
phosphate + CDP
show the reaction diagram
Saccharomyces fragilis
-
-
-
?
GDP + phosphate
phosphate + GDP
show the reaction diagram
Saccharomyces fragilis
-
-
-
?
IDP + phosphate
phosphate + IDP
show the reaction diagram
Saccharomyces fragilis
-
-
-
?
phosphate + adenosine 5'-phosphoamidate
ADP + NH4+
show the reaction diagram
22% reaction velocity compared to adenosine 5'-phosphosulfate
-
-
ir
phosphate + adenosine 5'-phosphofluoride
ADP + fluoride
show the reaction diagram
8% reaction velocity compared to adenosine 5'-phosphosulfate
-
-
?
phosphate + adenosine 5'-phosphosulfate
ADP + SO42-
show the reaction diagram
phosphate + diadenosine tetraphosphate
?
show the reaction diagram
2% reaction velocity compared to adenosine 5'-phosphosulfate
-
-
?
phosphate + diadenosine triphosphate
?
show the reaction diagram
phosphate + inosine 5'-phosphosulfate
IDP + SO42-
show the reaction diagram
UDP + phosphate
phosphate + UDP
show the reaction diagram
Saccharomyces fragilis
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphate + adenosine 5'-phosphosulfate
ADP + SO42-
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
Saccharomyces fragilis
-
slight inhibition
Ca2+
Saccharomyces fragilis
-
strong inhibition
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine 5'-phosphosulfate
-
substrate inhibition above 1 mM
AMP
Saccharomyces fragilis
-
-
arsenate
chromate
Saccharomyces fragilis
-
-
diacetyl
slow but complete inactivation
F-
Saccharomyces fragilis
-
-
N-ethylmaleimide
NaCl
Saccharomyces fragilis
-
slight inhibition
nitrate
Saccharomyces fragilis
-
-
p-chloromercuribenzoate
Phenylglyoxal
incomplete inactivation
phosphate
selenate
Saccharomyces fragilis
-
-
sulfate
vanadate
Saccharomyces fragilis
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
Saccharomyces fragilis
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25 - 26
adenosine 5'-phosphosulfate
0.0005
ADP
Saccharomyces fragilis
-
30C
0.0003
GDP
Saccharomyces fragilis
-
30C
0.0015 - 600
phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.6 - 6
adenosine 5'-phosphosulfate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11.7
phosphate
-
30C, pH 8
167
sulfate
-
4 mM adenosine 5'-phosphosulfate, 30C, pH 8
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.03
-
purified enzyme, 30C, pH 7.5
0.043
-
cytoplasm, pH 8, 80C
0.8
-
purified enzyme, 30C, pH 8
16
Saccharomyces fragilis
-
purified enzyme, 30C
2100
purified enzyme, pH 7.6
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
Saccharomyces fragilis
-
-
8.5 - 9
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2 - 6.8
Hint4 degrades adenosine 5'-phosphosulfate phosphorolytically twice as fast at pH 4.2 than at pH 6.8
5.5 - 7.5
Saccharomyces fragilis
-
rapid loss of activity above pH 7.5 with UDP
5.5 - 8.5
Saccharomyces fragilis
-
with ADP, CDP and GDP
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
Saccharomyces fragilis
-
-
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41390
calculated from DNA sequence
120000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
rapid loss of activity below pH 6 or above pH 8
643454
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
Saccharomyces fragilis
-
84% loss of activity within 1 min
60
-
69% loss of activity within 5 min
70
-
no activity after 5 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
purified enzyme stabilized by 5 mg/ml bovine serum albumin
very heat labile
-
very labile , 50% loss of activity by four times freeze and thaw
-
very labile in crude extract, partially stabilized by addition of EDTA and 2-mercaptoethanol
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18C stable for several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE