Information on EC 2.7.7.46 - gentamicin 2''-nucleotidyltransferase

Word Map on EC 2.7.7.46
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.7.46
-
RECOMMENDED NAME
GeneOntology No.
gentamicin 2''-nucleotidyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
nucleoside triphosphate + gentamicin = diphosphate + 2''-nucleotidylgentamicin
show the reaction diagram
ATP, dATP, CTP, ITP and GTP can act as donors; kanamycin, tobramycin and sisomicin can also act as acceptors. The nucleotidyl residue is transferred to the 2-hydroxy of the 3-amino-3-deoxy-D-glucose moiety in the antibiotic
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
NTP:gentamicin 2''-nucleotidyltransferase
ATP, dATP, CTP, ITP and GTP can act as donors; kanamycin, tobramycin and sisomicin can also act as acceptors. The nucleotidyl residue is transferred to the 2-hydroxy of the 3-amino-3-deoxy-D-glucose moiety in the antibiotic.
CAS REGISTRY NUMBER
COMMENTARY hide
62213-33-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain JR66/W677
-
-
Manually annotated by BRENDA team
strain W677/pMY10
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenosine 5'-mono-O-phosphorothiolate + tobramycin
diphosphate + 2''-(adenosine-5'-thiophosphoryl)-tobramycin
show the reaction diagram
-
-
-
-
r
ATP + dibekacin
diphosphate + 2''-adenylyldibekacin
show the reaction diagram
-
-
-
-
r
ATP + dideoxykanamycin B
diphosphate + 3',4'-dideoxykanamycin B-2''-adenylate
show the reaction diagram
ATP + gentamicin A
diphosphate + 2''-adenylylgentamicin A
show the reaction diagram
ATP + gentamicin C1
diphosphate + 2''-adenylylgentamicin C1
show the reaction diagram
ATP + gentamicin C1a
diphosphate + 2''-adenylylgentamicin C1a
show the reaction diagram
ATP + gentamicin C2
diphosphate + 2''-adenylylgentamicin C2
show the reaction diagram
-
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
-
-
-
ATP + kanamycin A
diphosphate + 2''-adenylylkanamycin A
show the reaction diagram
ATP + neomycin C
diphosphate + 2''-adenylylneomycin C
show the reaction diagram
ATP + netilmicin C
diphosphate + 2''-adenylylnetilmicin C
show the reaction diagram
-
-
-
-
r
ATP + sisomicin
diphosphate + 2''-adenylylsisomicin
show the reaction diagram
ATP + tobramycin
diphosphate + 2''-(adenosine-5'-phosphoryl)-tobramycin
show the reaction diagram
-
-
-
-
r
ATP + tobramycin
diphosphate + 2''-adenylyltobramycin
show the reaction diagram
CTP + tobramycin
diphosphate + 2''-adenylyltobramycin
show the reaction diagram
-
-
-
-
r
dATP + tobramycin
diphosphate + 2''-adenylyltobramycin
show the reaction diagram
-
-
-
-
r
dCTP + tobramycin
diphosphate + 2''-adenylyltobramycin
show the reaction diagram
-
-
-
-
r
dGTP + tobramycin
diphosphate + 2''-adenylyltobramycin
show the reaction diagram
-
-
-
-
r
dTTP + tobramycin
diphosphate + 2''-adenylyltobramycin
show the reaction diagram
-
-
-
-
r
GTP + tobramycin
diphosphate + 2''-adenylyltobramycin
show the reaction diagram
-
-
-
-
r
additional information
?
-
-
amikacin, neomycin, lividomycin and butirosin A and B are no substrates
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + gentamicin A
diphosphate + 2''-adenylylgentamicin A
show the reaction diagram
-
gentamicin A, C1, C1a, or C2 can act as acceptors, dATP, CTP, ITP or GTP can act as donors
-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
shows enzymatic activity only in presence of cations, optimal concentration 1.0 mM
additional information
-
K+ and Na+ have almost no effect, NH4+ has a low enhancing effect
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
above 0.5 mM
gentamycin C1
-
-
MgATP2-
-
metal chelates being the true substrates, free nucleotides being inhibitors
MgCTP2-
-
metal chelates being the true substrates, free nucleotides being inhibitors
MgdATP2-
-
metal chelates being the true substrates, free nucleotides being inhibitors
MgdCTP2-
-
metal chelates being the true substrates, free nucleotides being inhibitors
MgdGTP2-
-
metal chelates being the true substrates, free nucleotides being inhibitors
MgdTTP2-
-
metal chelates being the true substrates, free nucleotides being inhibitors
MgGTP2-
-
; metal chelates being the true substrates, free nucleotides being inhibitors
neomycin C
-
dead-end inhibition, uncompetitive versus MgATP2-
netilmicin
-
substrate inhibition
sisomicin
-
uncompetitive substrate inhibition
tobramycin
-
substrate inhibition, uncompetitive versus MgATP2-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.404
MgATP2-
-
pH 9.0, 25°C
2.008
MgCTP2-
-
pH 9.0, 25°C
0.03
MgdATP2-
-
pH 9.0, 25°C
0.09
MgdCTP2-
-
pH 9.0, 25°C
0.03
MgdGTP2-
-
pH 9.0, 25°C
0.09
MgdTTP2-
-
pH 9.0, 25°C
0.00276
tobramycin
-
pH 9.0, 25°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.84
gentamycin C1
-
-
0.864
MgATP2-
-
pH 9.0, 25°C
0.404
MgCTP
-
pH 9.0, 25°C
-
0.011
MgdATP2-
-
pH 9.0, 25°C
0.047
MgdCTP
-
pH 9.0, 25°C
0.027
MgdGTP
-
pH 9.0, 25°C
0.145
MgdTTP
-
pH 9.0, 25°C
-
0.01
MgGTP2-
-
pH 9.0, 25°C
5.12
netilmicin
-
-
0.154
sisomicin
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.134
-
isoform I
0.274
-
isoform II
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.5
-
isoform I
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9.5
-
-
6.5 - 10
-
-
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7
-
2 related isoforms, isoelectric focusing
6
-
2 related isoforms, isoelectric focusing
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31500
-
isoform II, SDS-PAGE
32500
-
isoform I, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 31500, isoform II, SDS-PAGE; 1 * 32500, isoform I, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
highly unstable after purification
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, activity remains only for a few hours
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 related isoforms
-
plasmid pPK237, purified from an E. coli K12 host RC85F-
-
recombinant enzyme, purified from overexpressing E. coli BL21(DE3)
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ANT (2'')-Ia cloned and overexpressed in Escherichia coli BL21(DE3)
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine