Information on EC 2.7.7.34 - glucose-1-phosphate guanylyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria

EC NUMBER
COMMENTARY hide
2.7.7.34
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RECOMMENDED NAME
GeneOntology No.
glucose-1-phosphate guanylyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GTP + alpha-D-glucose 1-phosphate = diphosphate + GDP-glucose
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
GDP-glucose biosynthesis
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Starch and sucrose metabolism
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Amino sugar and nucleotide sugar metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
GTP:alpha-D-glucose-1-phosphate guanylyltransferase
Also acts, more slowly, on D-mannose 1-phosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
9033-13-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mast cell tumor, grown in LAF mice
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-
Manually annotated by BRENDA team
IFO 3439, IFO 3123
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Manually annotated by BRENDA team
IFO 3122, IFO 3430, IFO 3356
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Manually annotated by BRENDA team
IFO 3145
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Manually annotated by BRENDA team
IFO 3409
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Manually annotated by BRENDA team
IFO 3389
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Manually annotated by BRENDA team
IFO 3441
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Manually annotated by BRENDA team
IFO 3310
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Manually annotated by BRENDA team
IFO 3111
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Manually annotated by BRENDA team
strain AKU 2801
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Manually annotated by BRENDA team
strain AKU 2801
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dGTP + alpha-D-glucosamine 1-phosphate
diphosphate + dGDP-glucosamine
show the reaction diagram
very low activity
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-
?
dGTP + alpha-D-glucose 1-phosphate
diphosphate + dGDP-glucose
show the reaction diagram
very low activity
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?
GTP + alpha-D-glucosamine 1-phosphate
diphosphate + GDP-glucosamine
show the reaction diagram
best substrate
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-
?
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
show the reaction diagram
GTP + D-mannose 1-phosphate
diphosphate + GDP-mannose
show the reaction diagram
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less effective than alpha-D-glucose 1-phosphate
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r
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
best activating metal ion
Ni2+
activates slightly
Zn2+
highly activating
additional information
the metal cofactor requirements of the multifunctional enzyme differ with the substrates used, overview
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-mannose 1-phosphate
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0.07 mM: 18% inhibition in presence of 3.5 mM GDPglucose, 40% inhibition in presence of 0.7 mM GDPglucose, no inhibition in presence of more than 7 mM GDPglucose
EDTA
complete inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
diphosphate
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pH 7.4, 30C
0.1
GDPglucose
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pH 7.4, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.046
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0.2
with dGTP and alpha-D-glucose 1-phosphate, purified His-tagged recombinant enzyme, pH 7.6, 85C
0.5
with dGTP and alpha-D-glucosamine 1-phosphate, purified His-tagged recombinant enzyme, pH 7.6, 85C
5.2
with GTP and alpha-D-glucose 1-phosphate, purified His-tagged recombinant enzyme, pH 7.6, 85C
7.3
with GTP and alpha-D-glucosamine 1-phosphate, purified His-tagged recombinant enzyme, pH 7.6, 85C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2 - 7.7
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8.7
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pH 6: 65% of maximal activity, pH 8.7: 60% of maximal activity at pH 8.7
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
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no activity in fibrosarcomase and umbilical cord
Manually annotated by BRENDA team
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, dried acetone powder, stable for at least two weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
mast cell tumor, grown in LAF mice, partial
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recombinant His-tagged PH0925 protein from Escherichia coli strain BL21-Codon Plus(DE3)-RIL by nickel affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene PH0925, recombinant expression of His-tagged enzyme PH0925 in Escherichia coli strain BL21-Codon Plus(DE3)-RIL
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information