Information on EC 2.7.7.30 - fucose-1-phosphate guanylyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.7.30
-
RECOMMENDED NAME
GeneOntology No.
fucose-1-phosphate guanylyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GTP + beta-L-fucose 1-phosphate = diphosphate + GDP-L-fucose
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
-
-
Fructose and mannose metabolism
-
-
GDP-L-fucose biosynthesis II (from L-fucose)
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
GTP:beta-L-fucose-1-phosphate guanylyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9033-14-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ecotype Columbia (Col)
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-amino-6-chloropurine-beta-D-riboside 5'-triphosphate + L-fucose 1-phosphate
diphosphate + 2-amino-6-chloropurine-beta-D-riboside 5'-(alpha-L-fucosyl)diphosphate
show the reaction diagram
-
-
-
-
?
2-aminoadenosine triphosphate + L-fucose 1-phosphate
diphosphate + (2-aminoadenosine diphosphate)-L-fucose
show the reaction diagram
-
-
-
-
?
7-deazaguanosine triphosphate + L-fucose 1-phosphate
diphosphate + (7-deazaguanosine diphosphate)-L-fucose
show the reaction diagram
-
-
-
-
?
8-oxoguanosine triphosphate + L-fucose 1-phosphate
diphosphate + (8-oxoguanosine diphosphate)-L-fucose
show the reaction diagram
-
-
-
-
?
ATP + beta-L-fucose 1-phosphate
ADP-L-fucose + diphosphate
show the reaction diagram
-
-
-
?
ATP + L-fucose 1-phosphate
diphosphate + ADP-L-fucose
show the reaction diagram
diphosphate + GDP-L-fucose
GTP + L-fucose 1-phosphate
show the reaction diagram
-
-
-
r
GTP + 6,7-dideoxy-beta-L-galacto-hept-6-enopyranose 1-phosphate
GDP-6,7-dideoxy-beta-L-galacto-hept-6-enopyranose + diphosphate
show the reaction diagram
-
-
-
-
?
GTP + 6-azido-6-deoxy-beta-L-galactopyranose 1-phosphate
GDP-6-azido-6-deoxy-beta-L-galactopyranose + diphosphate
show the reaction diagram
-
-
-
-
?
GTP + 6-deoxy-6-fluoro-beta-L-galactopyranose 1-phosphate
GDP-6-deoxy-6-fluoro-beta-L-galactopyranose + diphosphate
show the reaction diagram
-
-
-
-
?
GTP + 6-O-methyl-beta-L-galactopyranose 1-phosphate
GDP-6-O-methyl-beta-L-galactopyranose + diphosphate
show the reaction diagram
-
-
-
-
?
GTP + alpha-D-(5-cyano)arabinopyranose 1-phosphate
GDP-alpha-D-(5-cyano)arabinopyranose + diphosphate
show the reaction diagram
-
-
-
-
?
GTP + alpha-D-arabinopyranose 1-phosphate
GDP-alpha-D-arabinopyranose + diphosphate
show the reaction diagram
GTP + alpha-D-mannose 1-phosphate
diphosphate + GDP-alpha-D-mannopyranose
show the reaction diagram
-
17.5% of activity with GDP-L-fucose
-
?
GTP + beta-L-fucose 1-phosphate
diphosphate + GDP-L-fucose
show the reaction diagram
-
-
-
-
?
GTP + beta-L-fucose 1-phosphate
GDP-L-fucose + diphosphate
show the reaction diagram
GTP + beta-L-galactopyranose 1-phosphate
GDP-beta-L-galactopyranose + diphosphate
show the reaction diagram
-
-
-
-
?
GTP + D-arabinose 1-phosphate
diphosphate + GDP-arabinose
show the reaction diagram
-
-
-
-
?
GTP + D-arabinose 1-phosphate
GDP-D-arabinopyranoside + diphosphate
show the reaction diagram
-
5-10% of activity with L-fucose
-
?
GTP + D-fructose 1-phosphate
diphosphate + GDP-fructose
show the reaction diagram
-
-
-
-
?
GTP + D-galactose 1-phosphate
diphosphate + GDP-galactose
show the reaction diagram
-
-
-
-
?
GTP + D-glucosamine 1-phosphate
diphosphate + GDP-glucosamine
show the reaction diagram
-
-
-
-
?
GTP + D-glucose 1,6-diphosphate
diphosphate + ?
show the reaction diagram
-
-
-
-
?
GTP + D-glucose 1-phosphate
diphosphate + GDP-glucose
show the reaction diagram
-
-
-
-
?
GTP + D-mannose 1-phosphate
diphosphate + GDP-mannose
show the reaction diagram
-
-
-
-
?
GTP + L-fucose 1-phosphate
diphosphate + GDP-L-fucose
show the reaction diagram
GTP + L-fucose 1-phosphate
GDP-L-fucose + diphosphate
show the reaction diagram
-
-
-
-
r
ITP + L-fucose 1-phosphate
diphosphate + IDP-L-fucose
show the reaction diagram
L-fucose 1-phosphate + GTP
GDP-L-fucose + diphosphate
show the reaction diagram
L-fucose-1-phosphate + GTP
GDP-L-fucose + diphosphate
show the reaction diagram
N1-methylguanosine triphosphate + L-fucose 1-phosphate
diphosphate + (N1-methylguanosine diphosphate)-L-fucose
show the reaction diagram
-
-
-
-
?
N2-methylguanosine triphosphate + L-fucose 1-phosphate
diphosphate + (N2-methylguanosine diphosphate)-L-fucose
show the reaction diagram
-
-
-
-
?
O6-methylguanosine triphosphate + L-fucose 1-phosphate
diphosphate + (O6-methylguanosine diphosphate)-L-fucose
show the reaction diagram
-
-
-
-
?
tricyclic guanosine triphosphate + L-fucose 1-phosphate
diphosphate + (tricyclic guanosine diphosphate)-L-fucose
show the reaction diagram
-
-
-
-
?
UTP + L-fucose 1-phosphate
diphosphate + UDP-L-fucose
show the reaction diagram
-
18.7% of activity with GTP
-
?
xanthosine triphosphate + L-fucose 1-phosphate
diphosphate + (xanthosine diphosphate)-L-fucose
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GTP + beta-L-fucose 1-phosphate
diphosphate + GDP-L-fucose
show the reaction diagram
-
-
-
-
?
GTP + L-fucose 1-phosphate
diphosphate + GDP-L-fucose
show the reaction diagram
GTP + L-fucose 1-phosphate
GDP-L-fucose + diphosphate
show the reaction diagram
-
-
-
-
r
L-fucose 1-phosphate + GTP
GDP-L-fucose + diphosphate
show the reaction diagram
L-fucose-1-phosphate + GTP
GDP-L-fucose + diphosphate
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
requires the presence of divalent metal cations at the active site
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diphosphate
-
addition of diphosphate strongly inhibits the formation of GDP-L-Fuc by rAtFKGPi
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.163
2-amino-6-chloropurine-beta-D-riboside 5'-triphosphate
-
pH 7.0, 37°C
0.21
2-aminoadenosine triphosphate
-
pH 7.0, 37°C
0.0754
7-deazaguanosine triphosphate
-
pH 7.0, 37°C
0.0636
8-oxoguanosine triphosphate
-
pH 7.0, 37°C
0.516 - 0.8
ATP
0.03
beta-L-fucose 1-phosphate
-
-
0.0854
D-Arabinose 1-phosphate
-
pH 7.0, 37°C
0.823
D-Fructose 1-phosphate
-
pH 7.0, 37°C
0.677
D-galactose 1-phosphate
-
pH 7.0, 37°C
0.828
D-glucosamine 1-phosphate
-
pH 7.0, 37°C
0.621
D-glucose 1,6-diphosphate
-
pH 7.0, 37°C
0.346
D-glucose 1-phosphate
-
pH 7.0, 37°C
0.542
D-mannose 1-phosphate
-
pH 7.0, 37°C
0.135
diphosphate
-
pH 7.8, 37°C
0.06
fucose 1-phosphate
-
pH 7.8, 37°C
0.12
GDP-L-fucose
0.012 - 0.633
GTP
0.0616
ITP
-
pH 7.0, 37°C
0.067
L-fucose 1-phosphate
-
pH 7.0, 37°C
0.052
L-fucose-1-phosphate
-
-
0.423
N1-methylguanosine triphosphate
-
pH 7.0, 37°C
1.263
N2-methylguanosine triphosphate
-
pH 7.0, 37°C
0.121
O6-methylguanosine triphosphate
-
pH 7.0, 37°C
0.232
tricyclic guanosine triphosphate
-
pH 7.0, 37°C
0.823
xanthosine triphosphate
-
pH 7.0, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.27
2-amino-6-chloropurine-beta-D-riboside 5'-triphosphate
Homo sapiens
-
pH 7.0, 37°C
0.32
2-aminoadenosine triphosphate
Homo sapiens
-
pH 7.0, 37°C
1.5
7-deazaguanosine triphosphate
Homo sapiens
-
pH 7.0, 37°C
2.6
8-oxoguanosine triphosphate
Homo sapiens
-
pH 7.0, 37°C
0.4
arabinose 1-phosphate
Homo sapiens
-
pH 7.0, 37°C
0.009 - 0.22
ATP
0.03
fructose 1-phosphate
Homo sapiens
-
pH 7.0, 37°C
0.23
Galactose 1-phosphate
Homo sapiens
-
pH 7.0, 37°C
0.21
glucosamine 1-phosphate
Homo sapiens
-
pH 7.0, 37°C
0.18
glucose 1,6-diphosphate
Homo sapiens
-
pH 7.0, 37°C
0.32
glucose 1-phosphate
Homo sapiens
-
pH 7.0, 37°C
0.001 - 2.6
GTP
1.2
ITP
Homo sapiens
-
pH 7.0, 37°C
3.6
L-fucose 1-phosphate
Homo sapiens
-
pH 7.0, 37°C
0.2
mannose 1-phosphate
Homo sapiens
-
pH 7.0, 37°C
0.02
N1-methylguanosine triphosphate
Homo sapiens
-
pH 7.0, 37°C
0.009
N2-methylguanosine triphosphate
Homo sapiens
-
pH 7.0, 37°C
0.09
O6-methylguanosine triphosphate
Homo sapiens
-
pH 7.0, 37°C
0.29
tricyclic guanosine triphosphate
Homo sapiens
-
pH 7.0, 37°C
0.03
xanthosine triphosphate
Homo sapiens
-
pH 7.0, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0036
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 7.8
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-
7.5
-
activity assay
7.8
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9.5
-
approx. 85% of maximal activity at pH 6.0, approx. 70% of maximal activity at pH 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
activity assay
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 45
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rAtFKGP, GDP-L-Fuc pyrophosphorylase activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.87
-
calculated, theoretical isoelectric point
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
high expression level
Manually annotated by BRENDA team
-
mature plant
Manually annotated by BRENDA team
mRNA detected by northern analysis
Manually annotated by BRENDA team
-
mature plant
Manually annotated by BRENDA team
-
mature plant, relatively high expression of AtFKGP mRNA
Manually annotated by BRENDA team
-
mature plant
Manually annotated by BRENDA team
-
D51 cell, clonal derivative of mouse L 929 cell
Manually annotated by BRENDA team
mRNA detected by northern analysis
Manually annotated by BRENDA team
-
seedling
Manually annotated by BRENDA team
-
mature plant
Manually annotated by BRENDA team
mRNA detected by northern analysis
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
61000
-
gel filtration
66600
-
-
116400
-
AtFKGP, calculated
118800
x * 118800, calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 118800, calculated
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapor diffusion at 20°C
-
to 2.6 A resolution. Space group P41212, a single enzyme monomer per asymmetric unit
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-16°C, 10 mM dithioerythritol, 24 hours, 30-35% loss of activity
-
-16°C, 24 hours, 80% loss of activity
-
-20°C, 50 mM sucrose, several months, no loss of activity
-
-20°C, 50% (w/v) glycerol, 1 mM PMSF, 0.2 M NaCl, 50 mM Tris-HCl buffer, pH 7.5, 1 month, no loss of activity
-
-20°C, recombinant protein with maltose-binding protein affinity tag, stable for at least three months
0°C, 24 hours, 85% loss of activity
-
4°C, 1mM PMSF, 0.2 M NaCl, 50 mM Tris-HCl buffer, pH 7.5, 1 day, loss of more than 50% activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
polyethylene glycol, DE-52, Phenyl-Sepharose, hydroxylapatite, Sephacryl S-300, Red-Sepharose, chromatofocusing
-
protamine sulfate, ammonium sulfate, calcium phosphate gel, Sephadex G-100, DEAE-cellulose, alumina C-gamma
-
recombinant protein with maltose-binding protein affinity tag
Sepharose FF column, the recombinant protein fused to thioredoxin and His tags is partially purified by chelating chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in BL21 (DE3) gold strain of Escherichia coli
-
expression in Saccharomyces cerevisiae
-
expression of cDNA in murine myeloma cell line, i.e. NSO cells, and in Sf9 cells
-
expresssion in Escherichia coli
into the vector pET-16b for expression in Escherichia coli cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D184A
-
the ratio of turnover number to Km-value (for GTP) is 1.02fold higher than the wild-type ratio
D299A
-
the ratio of turnover number to Km-value (for GTP) is 1.04fold higher than the wild-type ratio
D427A
-
the ratio of turnover number to Km-value (for GTP) is 1.06fold lower than the wild-type ratio
D77A
-
the ratio of turnover number to Km-value (for GTP) is 16600fold lower than the wild-type ratio
E289A
-
the ratio of turnover number to Km-value (for GTP) is 1.1fold lower than the wild-type ratio
E297A
-
the ratio of turnover number to Km-value (for GTP) is 19.5fold lower than the wild-type ratio
E297A/E432A
-
the ratio of turnover number to Km-value (for GTP) is 24900fold lower than the wild-type ratio
E432A
-
the ratio of turnover number to Km-value (for GTP) is 113fold lower than the wild-type ratio
E566A
-
the ratio of turnover number to Km-value (for GTP) is 1.06fold higher than the wild-type ratio
E574A
-
the ratio of turnover number to Km-value (for GTP) is 1.05fold lower than the wild-type ratio
K478A
-
the ratio of turnover number to Km-value (for GTP) is 1.13fold lower than the wild-type ratio
K479A
-
the ratio of turnover number to Km-value (for GTP) is 1.15fold lower than the wild-type ratio
K482A
-
the ratio of turnover number to Km-value (for GTP) is 166fold lower than the wild-type ratio
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis