Information on EC 2.7.6.4 - nucleotide diphosphokinase

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The expected taxonomic range for this enzyme is: Streptomyces

EC NUMBER
COMMENTARY hide
2.7.6.4
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RECOMMENDED NAME
GeneOntology No.
nucleotide diphosphokinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + nucleoside 5'-phosphate = AMP + 5'-phosphonucleoside 3'-diphosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diphosphate transfer
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SYSTEMATIC NAME
IUBMB Comments
ATP:nucleoside-5'-phosphate diphosphotransferase
The enzyme acts on the 5'-mono-, di- and triphosphate derivatives of purine nucleosides.
CAS REGISTRY NUMBER
COMMENTARY hide
53167-92-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP
AMP + adenosine-3'-diphosphate-5'-triphosphate
show the reaction diagram
ATP + ADP
AMP + adenosine 3'-diphosphate 5'-diphosphate
show the reaction diagram
ATP + AMP
AMP + adenosine 3'-phosphate 5'-diphosphate
show the reaction diagram
ATP + GDP
AMP + guanosine 3'-diphosphate 5'-diphosphate
show the reaction diagram
ATP + GMP
AMP + guanosine 3'-phosphate 5'-diphosphate
show the reaction diagram
ATP + GTP
AMP + guanosine-3'-diphosphate-5'-triphosphate
show the reaction diagram
ATP + IDP
AMP + inosine 3'-diphosphate 5'-diphosphate
show the reaction diagram
ATP + IMP
AMP + inosine 3'-diphosphate 5'-phosphate
show the reaction diagram
ATP + ITP
AMP + inosine 3'-diphosphate 5'-triphosphate
show the reaction diagram
dATP + ADP
dAMP + adenosine 3'-diphosphate 5'-diphosphate
show the reaction diagram
dATP + AMP
dAMP + adenosine 3'-diphosphate 5'-phosphate
show the reaction diagram
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-
-
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?
dATP + ApG
dAMP + ApGpp
show the reaction diagram
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-
-
-
?
dATP + ApUpG
dAMP + ApUpGpp
show the reaction diagram
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-
-
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?
dATP + ATP
dAMP + adenosine 3'-diphosphate 5'-triphosphate
show the reaction diagram
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-
-
-
?
dATP + beta-NADH
dAMP + ?
show the reaction diagram
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-
-
-
?
dATP + CTP
dAMP + cytidine 3'-diphosphate 5'-triphosphate
show the reaction diagram
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-
-
-
?
dATP + GDP
dAMP + guanosine 3'-diphosphate 5'-diphosphate
show the reaction diagram
dATP + GDP-glucose
dAMP + ?
show the reaction diagram
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-
-
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?
dATP + GMP
dAMP + guanosine 3'-diphosphate 5'-phosphate
show the reaction diagram
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-
-
-
?
dATP + GpA
dAMP + GpApp
show the reaction diagram
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-
-
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?
dATP + GpG
dAMP + GpGpp
show the reaction diagram
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?
dATP + GTP
dAMP + guanosine 3'-diphosphate 5'-triphosphate
show the reaction diagram
dATP + IDP
dAMP + inosine 3'-diphosphate 5'-triphosphate
show the reaction diagram
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-
-
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?
dATP + IMP
dAMP + inosine 3'-diphosphate 5'-phosphate
show the reaction diagram
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-
-
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?
dATP + ITP
dAMP + inosine 3'-diphosphate 5'-triphosphate
show the reaction diagram
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-
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?
dATP + UDP-glucose
dAMP + ?
show the reaction diagram
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?
dATP + UpG
dAMP + UpGpp
show the reaction diagram
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?
dATP + UTP
dAMP + uridine 3'-diphosphate 5'-triphosphate
show the reaction diagram
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?
pppApp + ADP
ppApp + ATP
show the reaction diagram
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?
pppApp + GDP
ppGpp + ATP
show the reaction diagram
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?
pppApp + GTP
pppGpp + ATP
show the reaction diagram
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32.2% of the activity with GTP as substrate
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?
additional information
?
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dinucleotide 3',5'-diphosphodiester linkages, namely pAppA, ppAppA, and pppAppA are formed as transient intermediate during the diphosphokinase-catalyzed reaction of ApppA, AppppA, and ApppppA leading to the synthesis of pAp, ppAp and pppAp, which in turn is further converted to pAp, respectively
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ni2+
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absolute requirement for bivalent cations, at pH 10.0 Mg2+ is most effective, while Mn2+, Co2+ and Zn2+ show little activity. At pH 7.0, Co2+ is most effective and Mg2+, Mn2+, Ni2+ and Fe2+ show little activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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5 mM, 98.5% inhibition
KMnO4
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1 mM, 76% inhibition
Na-diphosphate
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1 mM, 14% inhibition
SDS
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0.1%, 87% inhibition
tetracyclin
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0.5 mg/ml, 37% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bovine serum albumin
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0.01 mg/ml, stimulates
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Tween 40
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0.01-0.001%, stimulate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.44 - 16.67
ADP
2.78
AMP
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pH 10.0, 37C, reaction with ATP
0.2 - 0.46
GDP
0.89
GMP
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pH 10.0, 37C, reaction with ATP
0.47 - 1.75
GTP
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
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optimal pH with Co2+ as stimulating cation
7.5 - 8
9 - 9.5
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reaction activated by Co2+ and Zn2+
9.5 - 10
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Mg2+ as activating cation
10 - 10.5
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optimal pH with Mg2+ or Mn2+ as stimulating cation
10
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above, reaction activated by Mg2+ or Mn2+
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 55
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40C: 70% of maximal activity, 55C: about 85% of maximal activity, pH 10.0
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.85
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isoelectric focusing
6.9
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isoelectric focusing
7.7
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isoelectric focusing
8.3
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isoelectric focusing
9.2
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isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000
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gel filtration
21000
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gel filtration
23000
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gel filtration
24000
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glycerol gradient centrifugation
25000
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gel filtration
26000
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gel filtration
28000
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gel filtration
29000
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equilibrium sedimentation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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37C, 20 h, 70% loss of activity
645209
5.5 - 10.5
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37C, 20 h, stable
645209
7 - 11
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37C, 2 h, 90% of the original activity is retained
645214
11
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37C, 20 h, 80% loss of activity
645209
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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pH 7-11, 37C, over 90% of the original activity is retained after 2 h
40
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10 min, 50% loss of original activity, inactivation is prevented by addition of substrate, non-ionic surface active agents or protein
60
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30 min, more than 80% of the activity remains
65
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30 min, 75% residual activity
100
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pH 9-11, in 0.05 M glycine/NaOH buffer containing 0.01 M MgCl2, no significant loss of activity after 15 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
at low protein concentration, below 0.01 mg/ml, considerable loss in activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, 0.05 M glycine/NaOH buffer, pH 9.0, 0.01 M MgCl2, in 60% saturated ammonium sulfate, 1 mg protein per mol, stable for at least several months
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no measurable loss of activity is detected after several months in a refrigerator
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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