Reference on EC 2.7.6.3 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Volpe, F.; Ballantine, S.P.; Delves, C.J.
The multifunctional folic acid synthesis fas gene of Pneumocystis carinii encodes dihydroneopterin aldolase, hydroxymethyldihydropterin pyrophosphokinase and dihydropteroate synthase
Eur. J. Biochem.
216
449-458
1993
Pneumocystis carinii
Richey, D.P.; Brown, G.M.
The biosynthesis of folic acid. IX. Purification and properties of the enzymes required for the formation of dihydropteroic acid
J. Biol. Chem.
244
1582-1592
1969
Escherichia coli
Walter, R.D.; Knigk, E.
Biosynthesis of folic acid compounds in plasmodia. Purification and properties of the 7,8-dihydropteroate-synthesizing enzyme from Plasmodium chabaudi
Hoppe-Seyler's Z. Physiol. Chem.
355
431-437
1974
Plasmodium chabaudi
Walter, R.D.; Knigk, E.
7,8-Dihydropteroate-synthesizing enzyme from Plasmodium chabaudi
Methods Enzymol.
66
564-570
1980
Plasmodium chabaudi
Talarico, T.L.; Dev, I.K.; Dallas, W.S.; Ferone, R.; Ray, P.H.
Purification and partial characterization of 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase and 7,8-dihydropteroate synthase from Escherichia coli MC4100
J. Bacteriol.
173
7029-7032
1991
Escherichia coli
Mouillon, J.M.; Ravanel, S.; Douce, R.; Rebeille, F.
Folate synthesis in higher-plant mitochondria: coupling between the dihydropterin pyrophosphokinase and the dihydropteroate synthase activities
Biochem. J.
363
313-319
2002
Pisum sativum
Bock, L.; Bartels, R.
New method for the purification of 7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase (E.C. 2.7.6.3) from Escherichia coli
J. Chromatogr.
26
206-209
1983
Escherichia coli
-
Bermingham, A.; Bottomley, J.R.; Primrose, W.U.; Derrick, J.P.
Equilibrium and kinetic studies of substrate binding to 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Escherichia coli
J. Biol. Chem.
275
17962-17967
2000
Escherichia coli (P26281), Escherichia coli
Ballantine, S.P.; Volpe, F.; Delves, C.J.
The hydroxymethyldihydropterin pyrophosphokinase domain of the multifunctional folic acid synthesis Fas protein of Pneumocystis carinii expressed as an independent enzyme in Escherichia coli: refolding and characterization of the recombinant enzyme
Protein Expr. Purif.
5
371-378
1994
Escherichia coli, Pneumocystis carinii
Li, Y.; Gong, Y.; Shi, G.; Blaszczyk, J.; Ji, X.; Yan, H.
Chemical transformation is not rate-limiting in the reaction catalyzed by Escherichia coli 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
Biochemistry
41
8777-8783
2002
Escherichia coli
Li, Y.; Wu, Y.; Blaszczyk, J.; Ji, X.; Yan, H.
Catalytic roles of arginine residues 82 and 92 of Escherichia coli 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase: site-directed mutagenesis and biochemical studies
Biochemistry
42
1581-1588
2003
Escherichia coli
Hennig, M.; Dale, G.E.; D'Arcy, A.; Danel, F.; Fischer, S.; Gray, C.P.; Jolidon, S.; Muller, F.; Page, M.G.; Pattison, P.; Oefner, C.
The structure and function of the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Haemophilus influenzae
J. Mol. Biol.
287
211-219
1999
Haemophilus influenzae
Garcon, A.; Bermingham, A.; Lian, L.Y.; Derrick, J.P.
Kinetic and structural characterization of a product complex of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Escherichia coli
Biochem. J.
380
867-873
2004
Escherichia coli (P26281), Escherichia coli
Blaszczyk, J.; Li, Y.; Shi, G.; Yan, H.; Ji, X.
Dynamic roles of arginine residues 82 and 92 of Escherichia coli 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase: crystallographic studies
Biochemistry
42
1573-1580
2003
Escherichia coli (P26281), Escherichia coli
Blaszczyk, J.; Li, Y.; Wu, Y.; Shi, G.; Ji, X.; Yan, H.
Essential roles of a dynamic loop in the catalysis of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
Biochemistry
43
1469-1477
2004
Escherichia coli (P26281)
Li, Y.; Blaszczyk, J.; Wu, Y.; Shi, G.; Ji, X.; Yan, H.
Is the critical role of loop 3 of Escherichia coli 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase in catalysis due to loop-3 residues arginine-84 and tryptophan-89? Site-directed mutagenesis, biochemical, and crystallographic studies
Biochemistry
44
8590-8599
2005
Escherichia coli (P26281), Escherichia coli
Yang, R.; Lee, M.C.; Yan, H.; Duan, Y.
Loop conformation and dynamics of the Escherichia coli HPPK apo-enzyme and its binary complex with MgATP
Biophys. J.
89
95-106
2005
Escherichia coli (P26281), Escherichia coli
Lawrence, M.C.; Iliades, P.; Fernley, R.T.; Berglez, J.; Pilling, P.A.; Macreadie, I.G.
The three-dimensional structure of the bifunctional 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/dihydropteroate synthase of Saccharomyces cerevisiae
J. Mol. Biol.
348
655-670
2005
Saccharomyces cerevisiae
Kasekarn, W.; Sirawaraporn, R.; Chahomchuen, T.; Cowman, A.F.; Sirawaraporn, W.
Molecular characterization of bifunctional hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase from Plasmodium falciparum
Mol. Biochem. Parasitol.
137
43-53
2004
Plasmodium falciparum
Joensson, M.; Swedberg, G.
Hydroxymethyldihydropterin pyrophosphokinase from Plasmodium falciparum complements a folK-knockout mutant in E. coli when expressed as a separate polypeptide detached from dihydropteroate synthase
Mol. Biochem. Parasitol.
140
123-125
2005
Plasmodium falciparum
Fernley, R.T.; Iliades, P.; Macreadie, I.
A rapid assay for dihydropteroate synthase activity suitable for identification of inhibitors
Anal. Biochem.
360
227-234
2007
Saccharomyces cerevisiae (P53848)
Li, G.; Felczak, K.; Shi, G.; Yan, H.
Mechanism of the conformational transitions in 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase as revealed by NMR spectroscopy
Biochemistry
45
12573-12581
2006
Escherichia coli (P26281)
de Beer, T.A.; Louw, A.I.; Joubert, F.
Elucidation of sulfadoxine resistance with structural models of the bifunctional Plasmodium falciparum dihydropterin pyrophosphokinase-dihydropteroate synthase
Bioorg. Med. Chem.
14
4433-4443
2006
Plasmodium falciparum (Q27865)
Storozhenko, S.; Navarrete, O.; Ravanel, S.; De Brouwer, V.; Chaerle, P.; Zhang, G.F.; Bastien, O.; Lambert, W.; Rebeille, F.; Van Der Straeten, D.
Cytosolic hydroxymethyldihydropterin pyrophosphokinase/dihydropteroate synthase from Arabidopsis thaliana: a specific role in early development and stress response
J. Biol. Chem.
282
10749-10761
2007
Arabidopsis thaliana, Arabidopsis thaliana (Q1ENB6)
Garcon, A.; Levy, C.; Derrick, J.P.
Crystal structure of the bifunctional dihydroneopterin aldolase/6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Streptococcus pneumoniae
J. Mol. Biol.
360
644-653
2006
Streptococcus pneumoniae
Dittrich, S.; Mitchell, S.L.; Blagborough, A.M.; Wang, Q.; Wang, P.; Sims, P.F.; Hyde, J.E.
An atypical orthologue of 6-pyruvoyltetrahydropterin synthase can provide the missing link in the folate biosynthesis pathway of malaria parasites
Mol. Microbiol.
67
609-618
2008
Plasmodium falciparum, Toxoplasma gondii
Blaszczyk, J.; Li, Y.; Cherry, S.; Alexandratos, J.; Wu, Y.; Shaw, G.; Tropea, J.E.; Waugh, D.S.; Yan, H.; Ji, X.
Structure and activity of Yersinia pestis 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase as a novel target for the development of anti-plague therapeutics
Acta Crystallogr. Sect. D
D63
1169-1177
2007
Yersinia pestis (Q7CKD7), Yersinia pestis
Chhabra, S.; Newman, J.; Peat, T.S.; Fernley, R.T.; Caine, J.; Simpson, J.S.; Swarbrick, J.D.
Crystallization and preliminary X-ray analysis of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Staphylococcus aureus
Acta Crystallogr. Sect. F
66
575-578
2010
Staphylococcus aureus
Shi, G.; Shaw, G.; Li, Y.; Wu, Y.; Yan, H.; Ji, X.
Bisubstrate analog inhibitors of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase: new lead exhibits a distinct binding mode
Bioorg. Med. Chem.
20
4303-4309
2012
Escherichia coli (P26281)
Shi, G.; Shaw, G.; Liang, Y.H.; Subburaman, P.; Li, Y.; Wu, Y.; Yan, H.; Ji, X.
Bisubstrate analogue inhibitors of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase: New design with improved properties
Bioorg. Med. Chem.
20
47-57
2012
Escherichia coli (P26281)
Pemble, C.W.; Mehta, P.K.; Mehra, S.; Li, Z.; Nourse, A.; Lee, R.E.; White, S.W.
Crystal structure of the 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase-dihydropteroate synthase bifunctional enzyme from Francisella tularensis
PLoS ONE
5
e14165
2010
Francisella tularensis (A0A6B2JKF9), Francisella tularensis, Francisella tularensis LVS (A0A6B2JKF9)
Yan, H.; Ji, X.
Role of protein conformational dynamics in the catalysis by 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
Protein Pept. Lett.
18
328-335
2011
Escherichia coli (P26281)
Gao, K.; He, H.; Yang, M.; Yan, H.
Molecular dynamics simulations of the Escherichia coli HPPK apo-enzyme reveal a network of conformational transitions
Biochemistry
54
6734-6742
2015
Escherichia coli (P26281), Escherichia coli
Yun, M.K.; Hoagland, D.; Kumar, G.; Waddell, M.B.; Rock, C.O.; Lee, R.E.; White, S.W.
The identification, analysis and structure-based development of novel inhibitors of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
Bioorg. Med. Chem.
22
2157-2165
2014
Escherichia coli (P26281)
Shaw, G.X.; Li, Y.; Shi, G.; Wu, Y.; Cherry, S.; Needle, D.; Zhang, D.; Tropea, J.E.; Waugh, D.S.; Yan, H.; Ji, X.
Structural enzymology and inhibition of the bi-functional folate pathway enzyme HPPK-DHPS from the biowarfare agent Francisella tularensis
FEBS J.
281
4123-4137
2014
Francisella tularensis (Q5NGA7), Francisella tularensis SCHU S4 (Q5NGA7)
Dennis, M.L.; Chhabra, S.; Wang, Z.C.; Debono, A.; Dolezal, O.; Newman, J.; Pitcher, N.P.; Rahmani, R.; Cleary, B.; Barlow, N.; Hattarki, M.; Graham, B.; Peat, T.S.; Baell, J.B.; Swarbrick, J.D.
Structure-based design and development of functionalized mercaptoguanine derivatives as inhibitors of the folate biosynthesis pathway enzyme 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Staphylococcus aureus
J. Med. Chem.
57
9612-9626
2014
Staphylococcus aureus
Dennis, M.L.; Pitcher, N.P.; Lee, M.D.; DeBono, A.J.; Wang, Z.C.; Harjani, J.R.; Rahmani, R.; Cleary, B.; Peat, T.S.; Baell, J.B.; Swarbrick, J.D.
Structural basis for the selective binding of inhibitors to 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Staphylococcus aureus and Escherichia coli
J. Med. Chem.
59
5248-5263
2016
Staphylococcus aureus, Escherichia coli (P26281), Escherichia coli
Chitnumsub, P.; Jaruwat, A.; Talawanich, Y.; Noytanom, K.; Liwnaree, B.; Poen, S.; Yuthavong, Y.
The structure of Plasmodium falciparum hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase reveals the basis of sulfa resistance
FEBS J.
287
3273-3297
2020
Plasmodium falciparum (Q25704)
Yogavel, M.; Nettleship, J.E.; Sharma, A.; Harlos, K.; Jamwal, A.; Chaturvedi, R.; Sharma, M.; Jain, V.; Chhibber-Goel, J.; Sharma, A.
Structure of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase-dihydropteroate synthase from Plasmodium vivax sheds light on drug resistance
J. Biol. Chem.
293
14962-14972
2018
Plasmodium vivax (A5JZS1), Plasmodium vivax, Plasmodium vivax Salvador 1 (A5JZS1), Plasmodium vivax Salvador I (A5JZS1)
Gao, K.; Jia, Y.; Yang, M.
A network of conformational transitions revealed by molecular dynamics simulations of the binary complex of Escherichia coli 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase with MgATP
Biochemistry
55
6931-6939
2016
Escherichia coli (P26281), Escherichia coli
Shi, G.; Shaw, G.X.; Zhu, F.; Tarasov, S.G.; Ji, X.
Bisubstrate inhibitors of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase Transition state analogs for high affinity binding
Bioorg. Med. Chem.
29
115847
2020
Escherichia coli (P26281)
Marimuthu, P.; Singaravelu, K.; Namasivayam, V.
Probing the binding mechanism of mercaptoguanine derivatives as inhibitors of HPPK by docking and molecular dynamics simulations
J. Biomol. Struct. Dyn.
35
3507-3521
2017
Staphylococcus aureus
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