Information on EC 2.7.4.23 - ribose 1,5-bisphosphate phosphokinase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.7.4.23
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RECOMMENDED NAME
GeneOntology No.
ribose 1,5-bisphosphate phosphokinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + alpha-D-ribose 1,5-bisphosphate = ADP + 5-phospho-alpha-D-ribose 1-diphosphate
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
aminomethylphosphonate degradation
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glyphosate degradation III
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Pentose phosphate pathway
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PRPP biosynthesis II
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SYSTEMATIC NAME
IUBMB Comments
ATP:ribose-1,5-bisphosphate phosphotransferase
This enzyme, found in NAD supression mutants of Escherichia coli, synthesizes 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) without the participation of EC 2.7.6.1, ribose-phosphate diphosphokinase. Ribose, ribose 1-phosphate and ribose 5-phosphate are not substrates, and GTP cannot act as a phosphate donor.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
K-12 strains; phnN
UniProt
Manually annotated by BRENDA team
phnN
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + ribose 1,5-bisphosphate
5-phospho-alpha-D-ribose 1-diphosphate + ADP
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.01
acitivity very low in a crude extract of strain HO1088/pTR553 before addition of IPTG
0.03
acitivity in a crude extract of strain HO1088/pHO500 after addition of IPTG
0.12
acitivity in a crude extract of strain HO1088/pTR553 after addition of IPTG
8.3
purified PhnN protein
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21410
calculated from the deduced nucleotide sequence of the allele specifying the histidine-tailed polypeptide
23500
gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purification of histidine-tailed ribose 1,5-bisphosphokinase, centrifugation, ultrasonic treatment, application to Co2+-containing Talon metal affinity resin, purified almost to homogeneity
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
into pSK50-delta A2 and then subcloned into pCAH56 and into pAH126
Show AA Sequence (1947 entries)
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