Information on EC 2.7.4.12 - T2-induced deoxynucleotide kinase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.7.4.12
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RECOMMENDED NAME
GeneOntology No.
T2-induced deoxynucleotide kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + dGMP (or dTMP) = ADP + dGDP (or dTDP)
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
purine deoxyribonucleosides salvage
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pyrimidine deoxyribonucleotide phosphorylation
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pyrimidine deoxyribonucleotides biosynthesis from CTP
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pyrimidine deoxyribonucleotides de novo biosynthesis I
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pyrimidine deoxyribonucleotides de novo biosynthesis II
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pyrimidine deoxyribonucleotides de novo biosynthesis III
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pyrimidine deoxyribonucleotides de novo biosynthesis IV
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Pyrimidine metabolism
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superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)
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purine metabolism
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SYSTEMATIC NAME
IUBMB Comments
ATP:(d)NMP phosphotransferase
dTMP and dAMP can act as acceptors; dATP can act as donor.
CAS REGISTRY NUMBER
COMMENTARY hide
37278-99-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
infected with bacteriophage T2
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 5-hydroxymethyl-dCMP
ADP + 5-hydroxymethyl-dCDP
show the reaction diagram
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phosphorylation at about half the rate of dGMP or dTMP
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r
ATP + dGMP
ADP + dGDP
show the reaction diagram
ATP + dTMP
ADP + dTDP
show the reaction diagram
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as good as dGMP
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r
dATP + dGMP
dADP + dGDP
show the reaction diagram
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phosphorylation at about 80-90% the rate of ATP
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r
dATP + dTMP
dADP + dTDP
show the reaction diagram
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phosphorylation at about 80-90% the rate of ATP
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-
r
additional information
?
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poor substrates are dUMP or 5-methyl-dCMP, no donor substrates are GTP, CTP, dGTP, dCTP, or dTTP, no acceptor substrates are dCMP, dAMP, GMP, or UMP
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + dGMP
ADP + dGDP
show the reaction diagram
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or dTMP
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r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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requirement, about 60% as effective as Mn2+, dGMP as substrate
Mg2+
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requirement, about 90% as effective as Mn2+, dGMP as substrate
Mn2+
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requirement, dGMP as substrate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-hydroxymethyl-dCMP
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dGMP or dTMP as substrate
dGMP
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dTMP or 5-hydroxymethyl-dCMP as substrate
dTMP
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dGMP or 5-hydroxymethyl-dCMP as substrate
additional information
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tryptic digestion inactivates
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.056
5-hydroxymethyl-dCMP
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pH 7.4, 37°C
0.82 - 5
ATP
0.085
dGMP
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pH 7.4, 37°C
0.278 - 0.313
dTMP
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pH 7.4, 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04 - 0.044
5-hydroxymethyl-dCMP
0.064 - 1.28
dGMP
0.25 - 0.314
dTMP
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
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dTMP or dGMP
8.6
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5-hydroxymethyl-dCMP
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.3
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pH 7: about 75% of maximal activity of 5-hydroxymethyl-dCMP, 84% of maximal activity dTMP or 87% of maximal activity of dGMP, pH 9.3: about 79% of maximal activity of dTMP, 83% of maximal activity of dGMP or 90% of maximal activity of 5-hydroxymethyl-dCMP
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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3 min, about 60% loss of activity at 37°C
642541
4.4
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37°C, 3 min, about 30% loss of activity, and about 45% loss of activity within 6 min
642541
4.7
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15 min, about 20% loss of activity at 37°C
642541
5.4
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15 min stable at 37°C
642541
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, crude preparation, at least a year
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4°C, partially purified preparation, at least 2 months
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at -10°C to 4°C, 1 mM EDTA, up to 20% loss of activity within 6 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
infected with bacteriophage T2
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