Information on EC 2.7.3.8 - ammonia kinase

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The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae

EC NUMBER
COMMENTARY hide
2.7.3.8
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RECOMMENDED NAME
GeneOntology No.
ammonia kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + NH3 = ADP + phosphoramide
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
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SYSTEMATIC NAME
IUBMB Comments
ATP:ammonia phosphotransferase
Has a wide specificity. In the reverse direction, N-phosphoglycine and N-phosphohistidine can also act as phosphate donors, and ADP, dADP, GDP, CDP, dTDP, dCDP, IDP and UDP can act as phosphate acceptors (in decreasing order of activity).
CAS REGISTRY NUMBER
COMMENTARY hide
37278-16-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + phosphoiodohistidine
ATP + iodohistidine
show the reaction diagram
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L-isomer and DL-racemate of phosphoiodohistidine
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-
?
ADP + phosphoramide
ATP + NH3
show the reaction diagram
-
-
-
-
?
CDP + phosphoramide
CTP + NH3
show the reaction diagram
-
-
-
-
?
dADP + phosphoramide
dATP + NH3
show the reaction diagram
-
-
-
-
?
dCDP + phosphoramide
dCTP + NH3
show the reaction diagram
-
-
-
-
?
GDP + phosphoramide
GTP + NH3
show the reaction diagram
-
-
-
-
?
IDP + phosphoramide
ITP + NH3
show the reaction diagram
-
-
-
-
?
TDP + phosphoramide
TTP + NH3
show the reaction diagram
-
-
-
-
?
UDP + phosphoramide
UTP + NH3
show the reaction diagram
-
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Fe2+
-
1.67 mM, 48% inhibition
Hg2+
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0.083 mM, 76% inhibition
iodoacetate
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1.67 mM, 12% inhibition
Mg2+
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133 mM, complete inhibition, inhibits reaction with phosphormaide or phosphoiodohistidine
Mn2+
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1.67 mM, 49% inhibition
NEM
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1.67 mM, 44% inhibition
oligomycin
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0.267 mM, 42% inhibition
p-chloromercuribenzoate
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0.83 mM, 95% inhibition
PO43-
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1.67 mM, 26% inhibition
Sodium arsenate
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1.67 mM, 65% inhibition
Sodium azide
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0.267 mM, 42% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.115
ADP
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pH 7.2
0.139
phosphoramide
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pH 7.2
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9
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reaction with phosphoiodohistidine, double pH maximum with peaks at 6.0 and 7.8
7 - 7.2
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reaction with phosphoramide
7.8
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reaction with phosphoiodohistidine, double pH maximum with peaks at 6.0 and 7.8
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
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pH 6.0: about 60% of maximal activity, pH 8.0: about 70% of maximal activity, reaction with phosphoramide
6.5 - 8.5
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pH 6.5: about 50% of maximal activity, pH 8.5: about 40% of maximal activity, reaction with phosphoiodohistidine
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
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10 min, denaturation above
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE