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ADP:protein phosphotransferase
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bifunctional regulatory protein
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PPDK regulatory protein 1
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PPDK regulatory protein 2
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PPDK regulatory protein isoform-2
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pyruvate orthophosphate dikinase regulatory protein
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pyruvate, orthophosphate dikinase regulatory protein
pyruvate, orthophosphate dikinase regulatory protein 1
pyruvate, orthophosphate dikinase regulatory protein 2
pyruvate,Pi dikinase regulatory protein
regulatory protein-like, ADP-dependent protein kinase
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PPDK regulatory protein
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PPDK regulatory protein
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PPDK regulatory protein
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bifunctional serine/threonine kinase-phosphatase
pyruvate, orthophosphate dikinase regulatory protein
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pyruvate, orthophosphate dikinase regulatory protein
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bifunctional protein kinase/phosphatase
pyruvate, orthophosphate dikinase regulatory protein 1
bifunctional
pyruvate, orthophosphate dikinase regulatory protein 1
bifunctional Ser/Thr protein kinase/protein phosphatase
pyruvate, orthophosphate dikinase regulatory protein 2
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bifunctional
pyruvate, orthophosphate dikinase regulatory protein 2
monofunctional Ser/Thr protein kinase
pyruvate,Pi dikinase regulatory protein
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pyruvate,Pi dikinase regulatory protein
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bifunctional, catalyses phosphorylation and dephosphorylation of pyruvate,Pi dikinase
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ADP + [pyruvate phosphate dikinase]
AMP + [pyruvate phosphate dikinase] phosphate
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?
ADP + [pyruvate, phosphate dikinase]
AMP + [pyruvate, phosphate dikinase] phosphate
C4-pyruvate,phosphate dikinase + ADP
[C4-pyruvate,phosphate dikinase]phosphate + AMP
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active
Thr456 phosphorylated (inactive)
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?
pyruvate, phosphate dikinase + ADP
[pyruvate, phosphate dikinase] phosphate + AMP
pyruvate,phosphate dikinase + ADP
[pyruvate,phosphate dikinase]phosphate + AMP
pyruvate,Pi dikinase + ADP
[pyruvate,Pi dikinase]phosphate + AMP
additional information
?
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ADP + [pyruvate, phosphate dikinase]
AMP + [pyruvate, phosphate dikinase] phosphate
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?
ADP + [pyruvate, phosphate dikinase]
AMP + [pyruvate, phosphate dikinase] phosphate
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reversible phosphorylation at Thr527 and Ser528, but not Thr309 and Ser506, of PPDK
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r
ADP + [pyruvate, phosphate dikinase]
AMP + [pyruvate, phosphate dikinase] phosphate
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i.e. recombinant His-tagged wild-type and mutant maize PPDKs as substrates, reversible phosphorylation at Thr527 and Ser528, but not Thr309 and Ser506, of PPDK. Phosphorylation is detected on wild-type PPDK, PPDK-T309A, and PPDK-S506A, but not on PPDK-T527A, PPDK-S528A, or PPDK-H529A
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r
pyruvate, phosphate dikinase + ADP
[pyruvate, phosphate dikinase] phosphate + AMP
active
inactive
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?
pyruvate, phosphate dikinase + ADP
[pyruvate, phosphate dikinase] phosphate + AMP
His458 phosphorylated (active), maize wild type and T456S mutant pyruvate, phosphate dikinase serve as substrate, T456V mutant pyruvate, phosphate dikinase serves not as substrate
His458 and Thr456 phosphorylated (inactive)
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?
pyruvate, phosphate dikinase + ADP
[pyruvate, phosphate dikinase] phosphate + AMP
His458 phosphorylated, active
His458 and Thr456 phosphorylated (inactive)
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?
pyruvate, phosphate dikinase + ADP
[pyruvate, phosphate dikinase] phosphate + AMP
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active
Thr residue phosphorylated (inactive)
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?
pyruvate, phosphate dikinase + ADP
[pyruvate, phosphate dikinase] phosphate + AMP
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His458 phosphorylated (active)
Thr456 and His458 phosphorylated (inactive)
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?
pyruvate, phosphate dikinase + ADP
[pyruvate, phosphate dikinase] phosphate + AMP
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His458 phosphorylated, active
His458 and Thr456 phosphorylated (inactive), T456S mutant protein substrate is also phosphorylated, H456N mutant protein substrat is not phosphorylated
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?
pyruvate,phosphate dikinase + ADP
[pyruvate,phosphate dikinase]phosphate + AMP
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His phosphorylated (active)
Thr and His phosphorylated (inactive)
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?
pyruvate,phosphate dikinase + ADP
[pyruvate,phosphate dikinase]phosphate + AMP
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His458 phosphorylated (activ), substrate mutant proteins T456Y and H458N are not phosphorylated (mutant protein T456S is phosphorylated), therefore a phosphorylated His458 in the substrate protein is necessary and the regulatory protein is functionally a Ser/Thr protein kinase
His458 and Thr456 phosphorylated (inactive)
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?
pyruvate,Pi dikinase + ADP
[pyruvate,Pi dikinase]phosphate + AMP
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His phosphorylated (active), substrate is dephosphorylated by conversion of pyruvate to phosphoenolpyruvate, dephosphorylated substrate serves not as substrate
Thr and His phosphorylated (inactive)
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?
pyruvate,Pi dikinase + ADP
[pyruvate,Pi dikinase]phosphate + AMP
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one His residue phosphorylated (active)
a His residue and a Thr residue phosphorylated (inactive)
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?
pyruvate,Pi dikinase + ADP
[pyruvate,Pi dikinase]phosphate + AMP
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substrate pyruvate,Pi dikinase is phosphorylated at a His residue and active; CDP shows 40% activity, GDP 35% activity and UDP 0% activity in comparison with ADP
phosphorylated at a Thr residue, inactive
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?
additional information
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PPDK regulatory protein (PDRP) is a unique bifunctional enzyme, catalyzes this light-dependent regulation by reversible phosphorylation of an active-site Thr527 in PPDK
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additional information
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modeling suggests that the two hydrogen bonds between the highly conserved residues Ser528 and Gly525 are required for PDRP-mediated phosphorylation of the active-site Thr527 of PPDK, substrate PPDK three-dimensional structure analysis, overview
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additional information
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isoform PDRP2 lacks the phosphotransferase activity of the bifunctional PDRP1 isoform except when PDRP2 in the assays is elevated 5-10fold
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additional information
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isoform PDRP2 lacks the phosphotransferase activity of the bifunctional PDRP1 isoform except when PDRP2 in the assays is elevated 5-10fold
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Chastain, C.J.; Botschner, M.; Harrington, G.E.; Thompson, B.J.; Mills, S.E.; Sarath, G.; Chollet, R.
Further analysis of maize C(4) pyruvate,orthophosphate dikinase phosphorylation by its bifunctional regulatory protein using selective substitutions of the regulatory Thr-456 and catalytic His-458 residues
Arch. Biochem. Biophys.
375
165-170
2000
Zea mays
brenda
Chastain, C.J.; Chollet, R.
Regulation of pyruvate, orthophosphate dikinase by ADP-/Pi-dependent reversible phosphorylation in C3 and C4 plants
Plant Physiol. Biochem.
41
523-532
2003
Zea mays
-
brenda
Burnell, J.N.; Chastain, C.J.
Cloning and expression of maize-leaf pyruvate, Pi dikinase regulatory protein gene
Biochem. Biophys. Res. Commun.
345
675-680
2006
Zea mays
brenda
Chastain, C.J.; Heck, J.W.; Colquhoun, T.A.; Voge, D.G.; Gu, X.Y.
Posttranslational regulation of pyruvate, orthophosphate dikinase in developing rice (Oryza sativa) seeds
Planta
224
924-934
2006
Oryza sativa
brenda
Chastain, C.J.; Xu, W.; Parsley, K.; Sarath, G.; Hibberd, J.M.; Chollet, R.
The pyruvate, orthophosphate dikinase regulatory proteins of Arabidopsis possess a novel, unprecedented Ser/Thr protein kinase primary structure
Plant J.
53
854-863
2008
Arabidopsis thaliana (O49562), Arabidopsis thaliana (Q9MAC9), Arabidopsis thaliana
brenda
Burnell, J.; Hatch, M.
Regulation of C4 photosynthesis: Identification of a catalytically important histidine residue and its role in the regulation of pyruvate,Pi dikinase
Arch. Biochem. Biophys.
231
175-182
1984
Zea mays
brenda
Burnell, J.; Hatch, M.
Regulation of C4 photosynthesis: Purification and properties of the protein catalyzing ADP-mediated inactivation and Pi-mediated activation of pyruvate,Pi dikinase
Arch. Biochem. Biophys.
237
490-503
1985
Zea mays
brenda
Budde, R.; Ernst, S.; Chollet, R.
Substrate specificity and regulation of the maize (Zea mays) leaf ADP: protein phosphotransferase catalysing phosphorylation/inactivation of pyruvate, orthophosphate dikinase
Biochem. J.
236
579-584
1986
Zea mays
brenda
Roeske, C.; Kutny, R.; Budde, R.; Chollet, R.
Sequence of the phosphothreonyl regulatory site peptide from inactive maize leaf pyruvate, orthophosphate dikinase
J. Biol. Chem.
263
6683-6687
1988
Zea mays
brenda
Chastain, C.; Thompson, B.; Chollet, R.
Maize recombinant C4-pyruvate,orthophosphate dikinase: Expression in Escherichia coli, partial purification, and characterization of the phosphorylatable protein
Photosynth. Res.
49
83-89
1996
Zea mays
brenda
Astley, H.; Parsley, K.; Aubry, S.; Chastain, C.; Burnell, J.; Webb, M.; Hibberd, J.
The pyruvate, orthophosphate dikinase regulatory proteins of Arabidopsis are both bifunctional and interact with the catalytic and nucleotide-binding domains of pyruvate, orthophosphate dikinase
Plant J.
68
1070-1080
2011
Arabidopsis thaliana, Arabidopsis thaliana (O23404)
brenda
Chen, Y.B.; Lu, T.C.; Wang, H.X.; Shen, J.; Bu, T.T.; Chao, Q.; Gao, Z.F.; Zhu, X.G.; Wang, Y.F.; Wang, B.C.
Posttranslational modification of maize chloroplast pyruvate orthophosphate dikinase reveals the precise regulatory mechanism of its enzymatic activity
Plant Physiol.
165
534-549
2014
Zea mays
brenda
Chastain, C.J.; Baird, L.M.; Walker, M.T.; Bergman, C.C.; Novbatova, G.T.; Mamani-Quispe, C.S.; Burnell, J.N.
Maize leaf PPDK regulatory protein isoform-2 is specific to bundle sheath chloroplasts and paradoxically lacks a Pi-dependent PPDK activation activity
J. Exp. Bot.
69
1171-1181
2018
Zea mays (A0A1D6IDV8), Zea mays
brenda
Jiang, L.; Chen, Y.; Zheng, J.; Chen, Z.; Liu, Y.; Tao, Y.; Wu, W.; Chen, Z.; Wang, B.
Structural basis of reversible phosphorylation by maize pyruvate orthophosphate dikinase regulatory protein
Plant Physiol.
170
732-741
2016
Zea mays
brenda