Information on EC 2.7.1.B26 - aminoglycoside 3'-phosphotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.B26
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
aminoglycoside 3'-phosphotransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GTP + gentamicin JI-20A = GDP + gentamicin JI-20A 3'-phosphate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
GTP:gentamicin JI-20A 3'-O-phosphotransferase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + amikacin
ADP + amikacin 3'-phosphate
show the reaction diagram
-
-
-
-
?
ATP + kanamycin A
ADP + kanamycin A 3'-phosphate
show the reaction diagram
-
-
-
-
?
ATP + neomycin B
ADP + neomycin B 3'-phosphate
show the reaction diagram
-
-
-
-
?
GTP + 1-deaminoneamine
GDP + 1-deaminoneamine 3'-phosphate
show the reaction diagram
-
-
-
?
GTP + 2'-deaminoneamine
GDP + 2'-deaminoneamine 3'-phosphate
show the reaction diagram
-
-
-
?
GTP + 3-deaminoneamine
GDP + 3-deaminoneamine 3'-phosphate
show the reaction diagram
-
-
-
?
GTP + 6'-deaminoneamine
GDP + 6'-deaminoneamine 3'-phosphate
show the reaction diagram
-
-
-
?
GTP + amikacin
GDP + amikacin 3'-phosphate
show the reaction diagram
GTP + gentamicin JI-20A
GDP + gentamicin JI-20A 3'-phosphate
show the reaction diagram
GTP + kanamycin A
GDP + kanamycin A 3'-phosphate
show the reaction diagram
GTP + kanamycin A
GDP + kanmycin A 3'-phosphate
show the reaction diagram
-
-
-
?
GTP + kanamycin B
GDP + kanamycin B 3'-phosphate
show the reaction diagram
GTP + neamine
GDP + neamine 3'-phosphate
show the reaction diagram
-
-
-
?
GTP + neomycin B
GDP + neomycin B 3'-phosphate
show the reaction diagram
additional information
?
-
an amino group on C-1 and either an amino or a hydroxyl group at the 2' and 6' positions are important for detoxification of aminoglycosides by the enzyme
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide
-
10 mM, 2% residual activity
4-chloromercuribenzoic acid
-
0.5 mM, 3% residual activity
diethyldicarbonate
-
2.5 mM, 10% residual activity
kanamycin A
-
-
N-ethylmaleimide
-
0.5 mM, 47% residual activity
neomycin B
-
-
Phenylglyoxal
-
2.5 mM, 10% residual activity
Tetranitromethane
-
2.5 mM, 23% residual activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0518
1-deaminoneamine
-
pH not specified in the publication, temperature not specified in the publication
0.0359
2'-deaminoneamine
-
pH not specified in the publication, temperature not specified in the publication
-
0.0071
3-deaminoneamine
-
pH not specified in the publication, temperature not specified in the publication
-
0.0818
6'-deaminoneamine
-
pH not specified in the publication, temperature not specified in the publication
-
0.048 - 0.245
amikacin
0.00116 - 0.0335
ATP
0.00092 - 0.018
kanamycin A
0.012
kanamycin B
pH 7.5, 37°C
0.02
neamine
-
pH not specified in the publication, temperature not specified in the publication
0.00029 - 0.027
neomycin B
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.03
1-deaminoneamine
Enterococcus faecium
-
pH not specified in the publication, temperature not specified in the publication
0.39
2'-deaminoneamine
Enterococcus faecium
-
pH not specified in the publication, temperature not specified in the publication
-
1.16
3-deaminoneamine
Enterococcus faecium
-
pH not specified in the publication, temperature not specified in the publication
-
0.278
6'-deaminoneamine
Enterococcus faecium
-
pH not specified in the publication, temperature not specified in the publication
-
0.12 - 2.46
amikacin
0.35 - 3.75
ATP
0.125 - 3.36
kanamycin A
0.07
kanamycin B
Micromonospora echinospora
Q6QVT6
pH 7.5, 37°C
2
neamine
Enterococcus faecium
-
pH not specified in the publication, temperature not specified in the publication
0.122 - 2.46
neomycin B
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
100
1-deaminoneamine
Enterococcus faecium
-
pH not specified in the publication, temperature not specified in the publication
206100
160
2'-deaminoneamine
Enterococcus faecium
-
pH not specified in the publication, temperature not specified in the publication
206874
20
3-deaminoneamine
Enterococcus faecium
-
pH not specified in the publication, temperature not specified in the publication
206873
11
6'-deaminoneamine
Enterococcus faecium
-
pH not specified in the publication, temperature not specified in the publication
206875
2.5
amikacin
Micromonospora echinospora
Q6QVT6
pH 7.5, 37°C
1394
3.4 - 6.7
kanamycin A
789
5.8
kanamycin B
Micromonospora echinospora
Q6QVT6
pH 7.5, 37°C
3078
8.6
neomycin B
Micromonospora echinospora
Q6QVT6
pH 7.5, 37°C
2279
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.3
kanamycin
-
pH not specified in the publication, temperature not specified in the publication
1.68 - 6.7
kanamycin A
2
neamine
-
pH not specified in the publication, temperature not specified in the publication
1.84 - 6.95
neomycin B
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
x * 30000, SDS-PAGE and calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
protein contains no disulfide bonds
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H123A
-
mutation has minimal effects on phosphorylation of several aminoglycosides
H188A
-
mutation has minimal effects on phosphorylation of several aminoglycosides
H78A
-
mutation has minimal effects on phosphorylation of several aminoglycosides
H82A
-
mutation has minimal effects on phosphorylation of several aminoglycosides