Information on EC 2.7.1.B19 - geranylgeraniol kinase

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The expected taxonomic range for this enzyme is: Sulfolobus acidocaldarius

EC NUMBER
COMMENTARY hide
2.7.1.B19
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
geranylgeraniol kinase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + geranylgeraniol = ADP + geranylgeranyl phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
isoprenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
ATP:geranylgeraniol O-phosphotransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (2E,6E)-farnesol
ADP + (2E,6E)-farnesyl phosphate
show the reaction diagram
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7% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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?
ATP + all-trans-hexaprenol
ADP + all-trans-hexaprenyl phosphate
show the reaction diagram
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38% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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?
ATP + all-trans-octaprenol
ADP + all-trans-octaprenyl phosphate
show the reaction diagram
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24% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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?
ATP + geranylgeraniol
ADP + geranylgeranyl phosphate
show the reaction diagram
CTP + geranylgeraniol
CDP + geranylgeranyl phosphate
show the reaction diagram
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63% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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?
GTP + geranylgeraniol
GDP + geranylgeranyl phosphate
show the reaction diagram
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87% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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?
UTP + geranylgeraniol
UDP + geranylgeranyl phosphate
show the reaction diagram
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76% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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?
additional information
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neither geraniol nor (22E,6E,10Z,14Z,18Z,22Z,26Z,30Z,34Z,38Z)-undecaprenol are active as substrates
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + geranylgeraniol
ADP + geranylgeranyl phosphate
show the reaction diagram
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all-trans-geranylgeraniol seems to be converted to its monophosphate in the membrane, and this product moves to the cytosol to undergo a second phosphorylation
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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5 mM, activates. No activity in absence of cations
Mg2+
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5 mM, activates. No activity in absence of cations
Mn2+
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5 mM, activates. No activity in absence of cations
Ni2+
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5 mM, activates. No activity in absence of cations
Zn2+
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5 mM, activates. No activity in absence of cations
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.65
ATP
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pH 7.0, 55C, assay with membrane fraction
0.000027
geranylgeraniol
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pH 7.0, 55C, assay with membrane fraction
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70 - 90
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activity at 70% is about 40% of the activity at 90C, no activity below 40C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
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1 h, about 15% loss of activity
95
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1 h, more than 90% loss of activity