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Information on EC 2.7.1.B19 - geranylgeraniol kinase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Sulfolobus acidocaldarius
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2.7.1.B19
preliminary BRENDA-supplied EC number
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ATP + geranylgeraniol = ADP + geranylgeranyl phosphate
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isoprenoid biosynthesis
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ATP:geranylgeraniol O-phosphotransferase
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brenda
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ATP + (2E,6E)-farnesol
ADP + (2E,6E)-farnesyl phosphate
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7% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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ATP + all-trans-hexaprenol
ADP + all-trans-hexaprenyl phosphate
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38% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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ATP + all-trans-octaprenol
ADP + all-trans-octaprenyl phosphate
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24% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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ATP + geranylgeraniol
ADP + geranylgeranyl phosphate
CTP + geranylgeraniol
CDP + geranylgeranyl phosphate
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63% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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GTP + geranylgeraniol
GDP + geranylgeranyl phosphate
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87% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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UTP + geranylgeraniol
UDP + geranylgeranyl phosphate
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76% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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additional information
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neither geraniol nor (22E,6E,10Z,14Z,18Z,22Z,26Z,30Z,34Z,38Z)-undecaprenol are active as substrates
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ATP + geranylgeraniol
ADP + geranylgeranyl phosphate
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all-trans-geranylgeraniol seems to be converted to its monophosphate in the membrane, and this product moves to the cytosol to undergo a second phosphorylation
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ATP + geranylgeraniol
ADP + geranylgeranyl phosphate
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all-trans-geranylgeraniol and ATP are the best substrates, activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes. No formation of geranylgeranyl diphosphate is observed
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ATP + geranylgeraniol
ADP + geranylgeranyl phosphate
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all-trans-geranylgeraniol seems to be converted to its monophosphate in the membrane, and this product moves to the cytosol to undergo a second phosphorylation
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Co2+
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5 mM, activates. No activity in absence of cations
Mg2+
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5 mM, activates. No activity in absence of cations
Mn2+
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5 mM, activates. No activity in absence of cations
Ni2+
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5 mM, activates. No activity in absence of cations
Zn2+
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5 mM, activates. No activity in absence of cations
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0.65
ATP
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pH 7.0, 55°C, assay with membrane fraction
0.000027
geranylgeraniol
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pH 7.0, 55°C, assay with membrane fraction
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70 - 90
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activity at 70% is about 40% of the activity at 90°C, no activity below 40°C
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brenda
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80
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1 h, about 15% loss of activity
95
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1 h, more than 90% loss of activity
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Ohnuma, S.; Watanabe, M.; Nishino, T.
Identification and characterization of geranylgeraniol kinase and geranylgeranyl phosphate kinase from the archaebacterium Sulfolobus acidocaldarius
J. Biochem.
119
541-547
1996
Sulfolobus acidocaldarius
brenda
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