Information on EC 2.7.1.B19 - geranylgeraniol kinase

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The expected taxonomic range for this enzyme is: Sulfolobus acidocaldarius

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EC NUMBER
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2.7.1.B19
preliminary BRENDA-supplied EC number
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RECOMMENDED NAME
GeneOntology No.
geranylgeraniol kinase
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REACTION
REACTION DIAGRAM
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ORGANISM
UNIPROT
LITERATURE
ATP + geranylgeraniol = ADP + geranylgeranyl phosphate
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
isoprenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
ATP:geranylgeraniol O-phosphotransferase
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ORGANISM
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LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (2E,6E)-farnesol
ADP + (2E,6E)-farnesyl phosphate
show the reaction diagram
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7% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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-
?
ATP + all-trans-hexaprenol
ADP + all-trans-hexaprenyl phosphate
show the reaction diagram
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38% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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-
?
ATP + all-trans-octaprenol
ADP + all-trans-octaprenyl phosphate
show the reaction diagram
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24% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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-
?
ATP + geranylgeraniol
ADP + geranylgeranyl phosphate
show the reaction diagram
CTP + geranylgeraniol
CDP + geranylgeranyl phosphate
show the reaction diagram
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63% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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?
GTP + geranylgeraniol
GDP + geranylgeranyl phosphate
show the reaction diagram
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87% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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?
UTP + geranylgeraniol
UDP + geranylgeranyl phosphate
show the reaction diagram
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76% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes
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?
additional information
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neither geraniol nor (22E,6E,10Z,14Z,18Z,22Z,26Z,30Z,34Z,38Z)-undecaprenol are active as substrates
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
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LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + geranylgeraniol
ADP + geranylgeranyl phosphate
show the reaction diagram
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all-trans-geranylgeraniol seems to be converted to its monophosphate in the membrane, and this product moves to the cytosol to undergo a second phosphorylation
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?
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METALS and IONS
ORGANISM
UNIPROT
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LITERATURE
Co2+
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5 mM, activates. No activity in absence of cations
Mg2+
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5 mM, activates. No activity in absence of cations
Mn2+
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5 mM, activates. No activity in absence of cations
Ni2+
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5 mM, activates. No activity in absence of cations
Zn2+
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5 mM, activates. No activity in absence of cations
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INHIBITORS
ORGANISM
UNIPROT
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LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
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LITERATURE
IMAGE
0.65
ATP
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pH 7.0, 55°C, assay with membrane fraction
0.000027
geranylgeraniol
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pH 7.0, 55°C, assay with membrane fraction
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pH OPTIMUM
ORGANISM
UNIPROT
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LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
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LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
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LITERATURE
70 - 90
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activity at 70% is about 40% of the activity at 90°C, no activity below 40°C
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LOCALIZATION
ORGANISM
UNIPROT
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GeneOntology No.
LITERATURE
SOURCE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
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1 h, about 15% loss of activity
95
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1 h, more than 90% loss of activity
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LINKS TO OTHER DATABASES (specific for EC-Number 2.7.1.B19)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)