Information on EC 2.7.1.94 - acylglycerol kinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.1.94
-
RECOMMENDED NAME
GeneOntology No.
acylglycerol kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + acylglycerol = ADP + acyl-sn-glycerol 3-phosphate
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycerolipid metabolism
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-
Metabolic pathways
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-
SYSTEMATIC NAME
IUBMB Comments
ATP:acylglycerol 3-phosphotransferase
Acts on both 1- and 2-acylglycerols.
CAS REGISTRY NUMBER
COMMENTARY hide
62213-37-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
strain B
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Manually annotated by BRENDA team
identity of diacylglycerol kinase and monoacylglycerol kinase is established by employing purified enzyme and immunospecific antibody
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Manually annotated by BRENDA team
isozyme alpha
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 1,2-dioleoyl-sn-glycerol
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
recombinant enzyme
-
-
?
ATP + 1,2-dipalmitoylglycerol
ADP + 1,2-dipalmitoylglycerol 3-phosphate
show the reaction diagram
-
-
product identified as alpha-phosphatidic acid
?
ATP + 1-arachidonoylglycerol
ADP + 1-arachidonoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-arachidoylglycerol
ADP + 1-arachidoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-caproylglycerol
ADP + 1-caproylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-capryloylglycerol
ADP + 1-capryloylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-lauroylglycerol
ADP + 1-lauroylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-linolenoylglycerol
ADP + 1-linolenoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-linoleoylglycerol
ADP + 1-linoleoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-monoolein
ADP + 1-oleoylglycerol 3-phosphate
show the reaction diagram
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i.e. 1-monooleolylglyerol
-
-
?
ATP + 1-myristoylglycerol
ADP + 1-myristoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-oleoyl-sn-glycerol
ADP + 1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
recombinant enzyme
-
-
?
ATP + 1-palmitoleoylglycerol
ADP + 1-palmitoleoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 1-palmitoyl-sn-glycerol
ADP + 1-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
recombinant enzyme
-
-
?
ATP + 1-palmitoylglycerol
ADP + 1-palmitoylglycerol 3-phosphate
show the reaction diagram
ATP + 1-stearoyl-sn-glycerol
ADP + 1-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
recombinant enzyme
-
-
?
ATP + 1-stearoylglycerol
ADP + 1-stearoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-arachidonoyl glycerol
ADP + 2-arachidonoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-arachidonoyl-glycerol
ADP + 2-arachidonoyl-glycerol 3-phosphate
show the reaction diagram
ATP + 2-arachidonoylglycerol
ADP + 2-arachidonoylglycerol 3-phosphate
show the reaction diagram
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-
-
-
?
ATP + 2-arachidoylglycerol
ADP + 2-arachidoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-caproylglycerol
ADP + 2-caproylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-lauroylglycerol
ADP + 2-lauroylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-linolenoylglycerol
ADP + 2-linolenoylglycerol 3-phosphate
show the reaction diagram
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-
-
-
?
ATP + 2-linoleoylglycerol
ADP + 2-linoleoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-monoolein
ADP + 2-oleoyoglycerol 3-phosphate
show the reaction diagram
-
i.e. 2-monooleolylglyerol
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-
?
ATP + 2-palmitoleoylglycerol
ADP + 2-palmitoleoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-palmitoylglycerol
ADP + 2-palmitoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 2-stearoylglycerol
ADP + 2-stearoylglycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
ATP + acylglycerol
ADP + acyl-sn-glycerol 3-phosphate
show the reaction diagram
ATP + diacylglycerol
ADP + phosphatidic acid
show the reaction diagram
ATP + monoacylglycerol
ADP + lysophosphatidic acid
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + acylglycerol
ADP + acyl-sn-glycerol 3-phosphate
show the reaction diagram
ATP + diacylglycerol
ADP + phosphatidic acid
show the reaction diagram
-
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i.e. diacyl-glycerol 3-phosphate, regulation mechanism, overview
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?
ATP + monoacylglycerol
ADP + lysophosphatidic acid
show the reaction diagram
-
-
i.e. acyl-sn-glycerol 3-phosphate, regulation mechanism, overview
-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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5 mM, 60% as effective as Mg2+ in activation
Cu2+
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5 mM, 51% as effective as Mg2+ in activation
Fe2+
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5 mM, 60% as effective as Mg2+ in activation
Ni2+
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5 mM, 60% as effective as Mg2+ in activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoic acid)
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DTT prevents inhibition
NEM
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DTT prevents inhibition
octyl-beta-glucopyranoside
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inhibits the recombinant enzyme in cell extract of transfected NIH 3T3 cells
p-Chloromercuriphenylsulfonate
phosphatidylcholine
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inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
phosphatidylethanolamine
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inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
sphingomyelin
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inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cutsum
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no absolute requirement, optimal concentration: 0.1%
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glutathione
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stimulates
Phospholipids
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e.g. phosphatidylcholine, phosphatidylethanolamine or sphingomyelin, inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate
additional information
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protein peak eluted from hydroxylapatite by 25 mM phosphate activates and stabilizes
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4 - 7.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
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about 35% of maximal activity at pH 6.5 and pH 7.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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enzyme overexpression
Manually annotated by BRENDA team
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high expression level
Manually annotated by BRENDA team
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enzyme overexpression in early stage
Manually annotated by BRENDA team
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low expression in normal and very low expression level in tumor cells
Manually annotated by BRENDA team
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high expression level
Manually annotated by BRENDA team
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enzyme AGK is significantly upregulated in NPC cell lines and clinical specimens as indicated by real-time PCR and Western blotting, overview
Manually annotated by BRENDA team
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low expression level in normal and tumor cells
Manually annotated by BRENDA team
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proliferative diabetic retinopathy and proliferative vitreoretinopathy epiretinal membranes
Manually annotated by BRENDA team
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high expression level
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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proliferative diabetic retinopathy and proliferative vitreoretinopathy epiretinal membranes
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
72000
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x * 72000, SDS-PAGE, enzyme exists as a complex that is stabilized by 0.5 M NaCl and, on complete dissociation yields a major protein of MW 72000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 72000, SDS-PAGE, enzyme exists as a complex that is stabilized by 0.5 M NaCl and, on complete dissociation yields a major protein of MW 72000
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
DTT, EDTA or ATP stabilizes during purification
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protein peak eluted from hydroxylapatite by 25 mM phosphate activates and stabilizes
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant human His6-tagged DGK isozyme theta from COS-7 cells by nickel affinity chromatography
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recombinant murine His6-tagged enzyme AGK from COS-7 cells by nickel affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
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DNA and amino acid sequence determination and analysis, expression in human PC-3 cells and HEK-293 cells, in mouse NIH 3T3 cells, and in rat RH7777 cells
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gene agk, quantitative real-time PCR enzyme expression analysis
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gene agk, quantitative real-time PCR expression analysis of AGK
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gene agk, recombinant overexpression of FLAG-tagged AGK in ECa109 and KYSE510 cells
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recombinant expression of His6-tagged enzyme AGK from vector pSF-CMV-NH2-His-EKT3 in COS-7 cells
recombinant expression of human His6-tagged DGK isozyme theta from vector pSF-CMV-NH2-His-EKT3 in COS-7 cells; recombinant expression of human p3×FLAG-CMV-DGK isozyme delta2 in COS-7 cells; recombinant expression of human p3×FLAG-CMV-DGK isozyme epsilon in COS-7 cells; recombinant expression of human p3×FLAG-CMV-DGK isozyme eta1 in COS-7 cells; recombinant expression of human p3×FLAG-CMV-DGK isozyme gamma in COS-7 cells; recombinant expression of human p3×FLAG-CMV-DGK isozyme kappa in COS-7 cells; recombinant expression of human p3×FLAG-CMV-DGK isozyme zeta1 in COS-7 cells
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recombinant expression of pig p3×FLAG-CMV-DGK isozyme alpha in COS-7 cells
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recombinant expression of rat p3×FLAG-CMV-DGK isozyme beta in COS-7 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expressions of enzyme is significantly correlated with primary Gleason grade of cancer foci and capsular invasion
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G126E
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site-directed mutagenesis, mutation of the conserved glycine residue of the glycine-rich loop of the ATP binding site, catalytically inactive mutant
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
drug development
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the enzyme AGK-based mechanism for constitutive activation of JAK2/STAT3 signaling in solid tumors and may represent a therapeutic target
medicine