Information on EC 2.7.1.82 - ethanolamine kinase

Word Map on EC 2.7.1.82
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.7.1.82
-
RECOMMENDED NAME
GeneOntology No.
ethanolamine kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + ethanolamine = ADP + O-phosphoethanolamine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phopho group transfer
-
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
choline biosynthesis I
-
-
Glycerophospholipid metabolism
-
-
Metabolic pathways
-
-
phosphatidylethanolamine biosynthesis II
-
-
plasmalogen biosynthesis
-
-
phosphatidylethanolamine bioynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:ethanolamine O-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9075-78-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
NCIB 9205
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene At2g26830
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
pigeon
-
-
Manually annotated by BRENDA team
Flavobacterium rhenanum
NCIB 9157, enzyme formed only when amino alcohol serves as a substrate and when activated by ADP
-
-
Manually annotated by BRENDA team
chicken
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Hemidactylus sp.
lizard
-
-
Manually annotated by BRENDA team
catfish
-
-
Manually annotated by BRENDA team
NCIB 8185, enzyme formed only when amino alcohol serves as a substrate and when activated by ADP
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
3D7
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
i.e. Hypocrea jecorina, ATCC 26921, gene Treki1
-
-
Manually annotated by BRENDA team
i.e. Hypocrea jecorina, ATCC 26921, gene Treki1
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (R)-1-aminopropan-2-ol
ADP + (R)-1-aminopropyl 2-phosphate
show the reaction diagram
ATP + (R)-2-aminobutan-1-ol
ADP + (R)-2-aminobutyl phosphate
show the reaction diagram
ATP + (R)-2-aminopropan-1-ol
ADP + (R)-2-aminopropyl phosphate
show the reaction diagram
ATP + (S)-1-aminopropan-2-ol
ADP + (S)-1-aminopropyl 2-phosphate
show the reaction diagram
ATP + (S)-2-aminobutan-1-ol
ADP + (S)-2-aminobutyl phosphate
show the reaction diagram
ATP + (S)-2-aminopropan-1-ol
ADP + (S)-2-aminopropyl phosphate
show the reaction diagram
ATP + 2-amino-2-methylpropan-1-ol
ADP + 2-amino-2-methylpropan-1-ol phosphate
show the reaction diagram
ATP + 3-aminopropan-1-ol
ADP + 3-aminopropyl 1-phosphate
show the reaction diagram
ATP + 4-aminobutan-1-ol
ADP + 4-aminobutyl 1-phosphate
show the reaction diagram
60% of the activity with choline
-
-
?
ATP + choline
ADP + ?
show the reaction diagram
ATP + choline
ADP + choline phosphate
show the reaction diagram
-
-
-
?
ATP + diethanolamine
ADP + O2-[(2-hydroxyethyl)amino]ethyl phosphate
show the reaction diagram
ATP + DL-1-aminopropan-2-ol
ADP + DL-1-aminopropane 2-phosphate
show the reaction diagram
ATP + ethanolamine
ADP + O-phosphoethanolamine
show the reaction diagram
ATP + N,N-diethylethanolamine
ADP + N,N-diethyl-O-phosphoethanolamine
show the reaction diagram
ATP + N,N-dimethylethanolamine
ADP + N,N-dimethyl-O-phosphoethanolamine
show the reaction diagram
ATP + N-ethylethanolamine
ADP + N-ethyl-O-phosphoethanolamine
show the reaction diagram
ATP + N-methylethanolamine
ADP + N-methyl-O-phosphoethanolamine
show the reaction diagram
choline + ATP
ADP + O-phosphocholine
show the reaction diagram
-
ethanolamine kinase II, not ethanolamine kinase I
-
-
?
GTP + ethanolamine
GDP + O-phosphoethanolamine
show the reaction diagram
-
13% of the activity with ATP
-
-
-
ITP + ethanolamine
IDP + O-phosphoethanolamine
show the reaction diagram
-
45% of the activity with ATP
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + ethanolamine
ADP + O-phosphoethanolamine
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP
Flavobacterium rhenanum
-
stimulates
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
100% activity in the presence of 0.0015 mM Zn2+, depletion of zinc from the growth medium of wild type cells causes a 2fold increase in ethanolamine kinase activity when compared with cells grown in the presence of zinc
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
2,3-Dimercaptopropanol
-
-
2-amino-1-butanol
3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium]
SAR97276
Acetyl-beta-methylcholine chloride
-
-
Acetylcholine
-
-
betaine
Butyrylcholine iodide
-
-
butyrylthiocholine iodide
-
-
choline
choline phosphate
-
-
cysteine
-
-
EGTA
-
stimulation at 0.4-0.9 mM, inhibition at 1.8 mM
Ethanolaminephosphate
-
-
hemicholinium
-
-
hemicholinium-3
-
Inositol
-
decreases gene EKI1 expression, involving the transcription factors Ino2p, Ino4p, and Opi1p, the UASINO element of the EKI promotor is required for the repressive regulation, the inhibitory effect on the enzyme is correlated with a decrease of ethanolamine incorporation into CDP-ethanolamine pathway intermediates and into phosphatidylethanolamine and phosphatidylcholine
Methane sulfonylcholine chloride
-
-
MgATP2-
-
substrate inhibition
monomethylethanolamine
-
-
N,N-dimethylethanolamine
N-ethylmaleimide
-
-
N-Methyl-N-isopropylethanolamine
-
-
N-Methylethanolamine
-
-
Ni2+
-
inhibits activating effect of Mg2+
p-chloromercuribenzoate
phosphorylcholine
Phosphorylethanolamine
Propionylcholine iodide
-
-
SAR97276
bis-thiazolium compound designed as a choline analogue, affects ethanolamine kinase and choline kinase activities similarly. Inhibition is competitive and correlates with the impairment of cellular phosphatidylcholine biosynthesis
stearoyl-CoA
-
-
Succinylcholine chloride
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
stimulates
cysteine
-
stimulates
EGTA
-
stimulation at 0.4-0.9 mM, inhibition at 1.8 mM
glutathione
-
stimulates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0206 - 0.5
ATP
0.02 - 0.275
choline
0.36
DL-1-aminopropan-2-ol
Flavobacterium rhenanum
-
-
0.008 - 10
ethanolamine
1.5 - 10
MgATP2-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00016 - 12.9
ethanolamine
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.73
3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium]
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.46 - 1.36
2-amino-1-butanol
0.61 - 0.79
3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium]
0.46
hemicholinium-3
Plasmodium falciparum
Q8IIB7
pH 8.0, 37°C
0.79
SAR97276
Plasmodium falciparum
Q8IIB7
pH 8.0, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.3
pH 8.0, 37°C
1.398
-
pH 8.5
10.73
-
pH 8.5
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
-
ethanolamine kinase I
8 - 9.5
-
ethanolamine kinase II
8.5 - 10.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4 - 10
-
-
8 - 9
-
80% of maximum activity at pH 8.0 and pH 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
during metamorphosis
Manually annotated by BRENDA team
high expression level of isozyme CEK4 in male reproductive organs
Manually annotated by BRENDA team
-
germinating
Manually annotated by BRENDA team
-
high expression of isoform Eki2 at the time of sex-differentiation
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17000 - 19000
-
gel filtration, monomeric form
36000 - 37000
-
dimeric form, gel filtration
36000
-
ethanolamine kinase I, gel filtration
42000
-
2 * 42000, SDS-PAGE
43000
-
x * 47000, or x-y * 47000 + y * 43000, or x * 43000, active form consitsts of oligomers of alpha/beta isoforms, with relatively small parts of alpha/alpha or beta/beta homo-oligomers, SDS-PAGE
47000
-
x * 47000, or x-y * 47000 + y * 43000, or x * 43000, active form consitsts of oligomers of alpha/beta isoforms, with relatively small parts of alpha/alpha or beta/beta homo-oligomers, SDS-PAGE
48000
determined by SDS-PAGE and Western Blot analysis
49100
2 * 49100, calculated, 2 * 51655, MALDI-TOF
51655
2 * 49100, calculated, 2 * 51655, MALDI-TOF
70140
2 * 70140, calculated, 2 * 72614, MALDI-TOF
72614
2 * 70140, calculated, 2 * 72614, MALDI-TOF
86000
-
2 * 86000, SDS-PAGE
87000
-
gel filtration
90000
-
gel filtration
100000
gel filtration
109000
determined by gel filtration
110000
-
gel filtration
140000
gel filtration
160000
-
ethanolamine kinase II, gel filtration
190000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 40000, about, sequence calculation
monomer
-
1 * 17000-19000, SDS-PAGE, gel filtration, enzyme exists in monomeric and dimeric form
oligomer
-
x * 47000, or x-y * 47000 + y * 43000, or x * 43000, active form consitsts of oligomers of alpha/beta isoforms, with relatively small parts of alpha/alpha or beta/beta homo-oligomers, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
20 min, complete inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
inactivation by repeated freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or 0-4°C, crude cell extract, appreciable loss of activity
-
-20°C, 0.02 M phosphate buffer, pH 7.0, 2.9 mM DTT, protein concentration 1.0 mg/ml, ethanolamine kinase I, 2 days, 40% loss of activity
-
-20°C, 10 days, stable
-
0-4°C or -20°C, partially purified enzyme, 10-14 days, 50% loss of activity
-
4°C, 1 week, ethanolamine kinase I, 50% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
copurification of choline kinase and ethanolamine kinase
-
ethanolamine kinase I and II
-
recombinant protein; recombinant protein
using a HisTrap chelating and a gel filtration column
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in 293T cells
expressed in Escherichia coli strain strain DH5alpha
-
expression as GST-fusion protein in Escherichia coli strain BL21
-
expression in Escherichia coli
expression in Escherichia coli; expression in Escherichia coli
expression of the enzyme fused to the lacZ reporter gene in several yeast strains and in Escherichia coli strain DH5alpha, expression analysis in presence of inositol and choline in correlation to wild-type and mutated promotor
-
gene At2g26830, PCR-based genotyping of CEK family members CEK1-4
gene eki1, a Sp site at position (-40/-31) is essential for the basal transcription of this gene, quantitative real-time PCR transcription analysis of ek1 mRNA, Identification of important regulatory regions in the ek1 gene promoter by analysis of 4 promoter constructs, trichostatin A activates basal ek1 promoter activity via Sp(-40/-31) site
gene ETNK1, overexpression of wild-type and N244S mutant enzymes in murine Ba/F3 cells
gene Treki1, subcloningin Escherichia coli strain DH5alpha
-
into the vector pET24b for expression in Escherichia coli BL21DE3-pRIL cells
overview
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression levels increases during erythrocytic development
substantial induction of splicing variant ek2alpha mRNA expression in response to trichostatin A treatment in Hep-G2 cells, the elevated ek2alpha mRNA expression may be due to the accumu­lation of acetylated histones H3 and H4, located around the promoter regions. Trichostatin A may also induce the recruit­ment of euchromatic markers and RNA polymerase II to the transcription factor complex that binds to the promoter, thus facilitating gene transcription
treatment of HCT-116 cells with trichostatin A (TSA), a histone deacetylase inhibitor, significantly upregulates the eki1 promoter activity through the Sp(-40/-31) site and increases the endogenous expression of gene eki1. Trichostatin A increases the binding of Sp1, Sp3 and RNA polymerase II to the ek1 promoter in HCT116 cells, molecular mechanism, overview. The effect of TSA on ek1 promoter activity is cell-line specific as TSA treatment does not affect ek1 promoter activity in Hep-G2 cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D816V
naturally occuring mutation involved in adult systemic mastocytosis, chronic myelomonocytic leukemia, or eosinophilia
G245A
naturally occuring mutation involved in adult systemic mastocytosis,with chronic myelomonocytic leukemia and/or eosinophilia, inactive mutant
N244K
naturally occuring mutation involved in adult systemic mastocytosis, with chronic myelomonocytic leukemia and/or eosinophilia, inactive mutant
N244S
naturally occuring mutation involved in adult systemic mastocytosis, with chronic myelomonocytic leukemia and/or eosinophilia, inactive mutant
N244T
naturally occuring mutation involved in adult systemic mastocytosis, with chronic myelomonocytic leukemia and/or eosinophilia, inactive mutant
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
Show AA Sequence (132 entries)
Please use the Sequence Search for a specific query.