Information on EC 2.7.1.81 - hydroxylysine kinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.81
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RECOMMENDED NAME
GeneOntology No.
hydroxylysine kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GTP + 5-hydroxy-L-lysine = GDP + 5-phosphonooxy-L-lysine
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Lysine degradation
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
GTP:5-hydroxy-L-lysine O-phosphotransferase
Both the natural 5-hydroxy-L-lysine and its 5-epimer act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
9073-58-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
no activity in Homo sapiens
no activity in liver and kidney
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 5-hydroxy-L-lysine
ADP + O-phosphohydroxy-L-lysine
show the reaction diagram
catalytic efficiency is 22fold higher with GTP than with ATP
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?
GTP + 5-hydroxy-D-lysine
GDP + O-phosphohydroxy-D-lysine
show the reaction diagram
best substrate is the physiological form 5-hydroxy-L-lysine but human enzyme can also phosphorylate at a lower rate at least one other isomer
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?
GTP + 5-hydroxy-L-lysine
GDP + O-phosphohydroxy-L-lysine
show the reaction diagram
GTP + allohydroxy-L-lysine
?
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GTP + 5-hydroxy-L-lysine
GDP + O-phosphohydroxy-L-lysine
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Co2+
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requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Cu2+
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requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Fe2+
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requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Hg2+
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requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+
Mn2+
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requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+, strong inhibition in presence of 1 mM Mg2+
additional information
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K+, Li+ have no influence
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-Amino-5-hydroxyadipate
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4 isomers, 40 mM, strong inhibition
4,5-Dehydro-(trans)-L-lysine
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carnitine
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2 isomers
choline
D-ornithine
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ethanolamine
Hydroxy-L-pipecolate
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hydroxy-L-proline
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; 40 mM, strong
L-2,4-diamino-n-butyrate
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L-homoserine
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40 mM, weak inhibition
L-lysine
L-ornithine
Mn2+
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at 1 mM, in presence of 1 mM Mg2+, strong inhibition, but activation in absence of Mg2+
S-aminoethyl-L-cysteine
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Zn2+
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at 1 mM, in presence of 1 mM Mg2+, strong inhibition
additional information
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no inhibition by serine, threonine, and hydroxyproline
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0076 - 0.28
5-hydroxy-L-lysine
0.0238
5-hydroxy-lysine
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0.0062
allohydroxy-L-lysine
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pH 8.0, 37°C
0.81
ATP
pH 7.1, 30°C
0.0035
GTP
pH 7.1, 30°C
0.0056
hydroxy-L-lysine
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pH 8.0, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12 - 45
5-hydroxy-L-lysine
16046
5
ATP
Homo sapiens
A2RU49
pH 7.1, 30°C
4
110
GTP
Homo sapiens
A2RU49
pH 7.1, 30°C
37
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
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25% of maximal activity at pH 6.0, 35% of maximal activity at pH 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
frozen, little loss of activity for weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1200fold; partial
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli