Information on EC 2.7.1.80 - diphosphate-serine phosphotransferase

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The expected taxonomic range for this enzyme is: Propionibacterium freudenreichii subsp. shermanii

EC NUMBER
COMMENTARY hide
2.7.1.80
-
RECOMMENDED NAME
GeneOntology No.
diphosphate-serine phosphotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
diphosphate + L-serine = phosphate + O-phospho-L-serine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
diphosphate:L-serine O-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
37205-58-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
diphosphate + alpha-methylserine
phosphate + alpha-methyl-O-phospho-L-serine
show the reaction diagram
-
7% activity compared to L-serine
-
-
?
diphosphate + DL-homoserine
phosphate + O-phospho-DL-homoserine
show the reaction diagram
-
7% activity compared to L-serine
-
-
?
diphosphate + glycyl-L-serine
phosphate + glycyl-O-phospho-L-serine
show the reaction diagram
-
16% activity compared to L-serine
-
-
?
diphosphate + L-serine
phosphate + O-phospho-L-serine
show the reaction diagram
-
-
-
?
diphosphate + L-threonine
phosphate + O-phospho-L-threonine
show the reaction diagram
-
8% activity compared to L-serine
-
-
?
diphosphate + N-chloroacetyl-L-serine
phosphate + N-chloroacetyl-O-phospho-L-serine
show the reaction diagram
-
19% activity compared to L-serine
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
diphosphate + L-serine
phosphate + O-phospho-L-serine
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
slight stimulation, about 14% of activity compared to Mg2+
Co2+
-
slight stimulation, about 42% of activity compared to Mg2+
Cu2+
-
slight stimulation, about 14% of activity compared to Mg2+
Mn2+
-
slight stimulation, about 34% of activity compared to Mg2+
Zn2+
-
slight stimulation, about 14% of activity compared to Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-serine
-
20 mM, weak inhibition
DL-homoserine
-
100 mM, weak inhibition
glycerol
-
100 mM, weak inhibition
glycine
-
20% inhibition at 10 mM
Glycyl-L-serine
-
weak inhibition
L-alanine
-
35% inhibition at 10 mM
L-threonine
-
100 mM, weak inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13
Mg2+
-
1 mM diphosphate, pH 7.8, 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.4 - 7.8
-
60% of maximal activity at pH 5.4 and pH 7.8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
-
15 min, 46% remaining activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C o r-15°C, 0.02 M Tris buffer, pH 7.8, stable for several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE