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(2',3')-uridylic acid + uridine
uridine + UMP
-
about 10% of the activity with phenyl phosphate
-
-
?
1-naphthyl phosphate + uridine
?
-
-
-
-
?
2-naphthyl phosphate + uridine
?
-
-
-
-
?
3'-AMP + 2'-deoxycytidine
dCMP + adenosine
-
-
-
-
?
3'-AMP + 2'-deoxyguanosine
dGMP + adenosine
-
-
-
-
?
3'-AMP + 2'-deoxythymidine
dTMP + adenosine
-
-
-
-
?
3'-AMP + adenosine
AMP + adenosine
-
-
-
-
?
3'-AMP + cytidine
CMP + adenosine
3'-AMP + guanosine
GMP + adenosine
3'-AMP + inosine
IMP + adenosine
-
-
-
-
?
3'-AMP + uridine
adenosine + UMP
3'-AMP + uridine
UMP + adenosine
-
-
-
-
?
3'-CMP + thymidine
cytosine + TMP
-
20% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
3'-dAMP + thymidine
TMP + 3'-deoxyadenosine
-
56% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
3'-TMP + thymidine
thymidine + TMP
-
19% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
3'-UMP + uridine
?
-
-
-
-
?
4-nitrophenyl phosphate + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl) thymine
4-nitrophenol + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl) thymine 5-phosphate
-
80% maximum conversion to 5'-monophosphate
-
-
?
4-nitrophenyl phosphate + 1-(beta-D-ribofuranosyl)-2(1H)pyridone
4-nitrophenol + 1-(beta-D-ribofuranosyl)-2(1H)pyridone 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 1-(beta-D-ribofuranosyl)-4(1H)pyridone
4-nitrophenol + 1-(beta-D-ribofuranosyl)-4(1H)pyridone 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2'-bromo-2'-deoxyuridine
4-nitrophenol + 2'-bromo-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxy-2'-methyluridine
4-nitrophenol + 2'-deoxy-2'-methyluridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyadenosine
2'-deoxyadenosine 5'-phosphate + 2'-deoxyadenosine 3'-phosphate + 4-nitrophenol
4-nitrophenyl phosphate + 2'-deoxyadenosine
4-nitrophenol + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxycytidine
4-nitrophenol + 2'-deoxycytidine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyguanosine
4-nitrophenol + 2'-deoxyguanosine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyinosine
4-nitrophenol + 2'-deoxyinosine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2'-O-methylcytidine
4-nitrophenol + 2'-O-methylcytidine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 2-thiouridine
4-nitrophenol + 2-thiouridine 5'phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxy-3'-methyluridine
4-nitrophenol + 3'-deoxy-3'-methyluridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxyadenosine
4-nitrophenol + 3'-deoxyadenosine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxyadenosine
4-nitrophenol + 3'-deoxyadenosine 5'phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxyinosine
4-nitrophenol + 3'-deoxyinosine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 3'-fluoroinosine
4-nitrophenol + 3'-fluoro-IMP
-
-
-
-
?
4-nitrophenyl phosphate + 3'-O-methylcytidine
4-nitrophenol + 3'-O-methylcytidine 5'phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 3-(beta-D-ribofuranosyl)uric acid
4-nitrophenol + 3-(beta-D-ribofuranosyl)uric acid 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 5-bromouridine
4-nitrophenol + 5-bromouridine 5'phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 5-chlorouridine
4-nitrophenol + 5-chlorouridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 5-hydroxyuridine
4-nitrophenol + 5-hydroxyuridine 5'phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 5-iodouridine
4-nitrophenol + 5-iodouridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 6-chloro-9-(beta-D-ribofuranosyl)purine
4-nitrophenol + 6-chloro-9-(beta-D-ribofuranosyl)purine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 7-methylguanosine
4-nitrophenol + 7-methylguanosine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + 9-(beta-D-ribofuranosyl)purine
4-nitrophenol + 9-(beta-D-ribofuranosyl)purine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + adenosine
4-nitrophenol + AMP
-
-
-
-
?
4-nitrophenyl phosphate + adenosine
5'-AMP + 4-nitrophenol
4-nitrophenyl phosphate + beta-L-2',3'-dideoxycytidine
4-nitrophenol + beta-L-2',3'-dideoxycytidine 5'-phosphate
-
38% maximum conversion to 5'-monophosphate
-
-
?
4-nitrophenyl phosphate + beta-L-3'-deoxy-2',3'-didehydrothymidine
4-nitrophenol + beta-L-3'-deoxy-2',3'-didehydrothymidine 5'-phosphate
-
60% maximum conversion to 5'-monophosphate
-
-
?
4-nitrophenyl phosphate + beta-L-3'-deoxy-3'-chlorothymidine
4-nitrophenol + beta-L-3'-deoxy-3'-chlorothymidine 5'-phosphate
-
90% maximum conversion to 5'-monophosphate
-
-
?
4-nitrophenyl phosphate + beta-L-thymidine
4-nitrophenol + beta-L-thymidine 5'-phosphate
-
35% maximum conversion to 5'-monophosphate
-
-
?
4-nitrophenyl phosphate + beta-pseudouridine
4-nitrophenol + beta-pseudouridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + cytidine
4-nitrophenol + CMP
-
-
-
-
?
4-nitrophenyl phosphate + deazauridine
4-nitrophenol + deazauridine 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + formycin B
4-nitrophenol + ?
-
-
-
-
?
4-nitrophenyl phosphate + guanosine
4-nitrophenol + GMP
-
-
-
-
?
4-nitrophenyl phosphate + inosine
4-nitrophenol + IMP
4-nitrophenyl phosphate + O2,2'-anhydro-beta-L-5-methyluridine
4-nitrophenol + O2,2'-anhydro-beta-L-5-methyluridine 5'-phosphate
-
65% maximum conversion to 5'-monophosphate
-
-
?
4-nitrophenyl phosphate + thymidine
4-nitrophenol + TMP
4-nitrophenyl phosphate + thymidine 3'-[(4-nitrophenyl)-phosphate]
4-nitrophenol + thymidine 3'-[(4-nitrophenyl)-phosphate] 5'-phosphate
-
-
-
-
?
4-nitrophenyl phosphate + uridine
4-nitrophenol + 5'-UMP
4-nitrophenyl phosphate + xanthosine
4-nitrophenol + XMP
-
-
-
-
?
5'-adenylic acid + uridine
adenosine + UMP
-
70% of the activity with phenyl phosphate
-
-
?
5'-AMP + adenosine
AMP + adenosine
-
-
-
-
?
5'-AMP + cytidine
CMP + adenosine
-
-
-
-
?
5'-AMP + guanosine
GMP + adenosine
-
-
-
-
?
5'-AMP + inosine
IMP + adenosine
-
-
-
-
?
5'-AMP + thymidine
adenosine + TMP
-
72% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
5'-AMP + uridine
UMP + adenosine
-
-
-
-
?
5'-CMP + adenosine
AMP + cytidine
-
-
-
-
?
5'-CMP + cytidine
CMP + cytidine
-
-
-
-
?
5'-CMP + guanosine
GMP + cytidine
-
-
-
-
?
5'-CMP + inosine
IMP + cytidine
-
-
-
-
?
5'-CMP + thymidine
cytidine + TMP
-
92% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
5'-CMP + uridine
UMP + cytidine
-
-
-
-
?
5'-dAMP + thymidine
5'-deoxyadenosine + TMP
-
-
-
-
?
5'-dCMP + thymidine
2'-deoxycytidine + TMP
-
46% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
5'-dGMP + thymidine
TMP + 2'-deoxyguanosine
-
65% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
5'-dTMP + thymidine
5'-deoxythymidine + TMP
-
-
-
-
?
5'-GMP + adenosine
AMP + guanosine
-
-
-
-
?
5'-GMP + cytidine
CMP + guanosine
-
-
-
-
?
5'-GMP + inosine
IMP + guanosine
-
-
-
-
?
5'-GMP + thymidine
TMP + guanosine
-
61% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
5'-GMP + uridine
UMP + guanosine
-
-
-
-
?
5'-IMP + adenosine
AMP + inosine
-
-
-
-
?
5'-IMP + cytidine
CMP + inosine
-
-
-
-
?
5'-IMP + guanosine
GMP + inosine
-
-
-
-
?
5'-IMP + inosine
IMP + inosine
-
-
-
-
?
5'-IMP + uridine
UMP + inosine
-
-
-
-
?
5'-UMP + adenosine
AMP + uridine
-
-
-
-
?
5'-UMP + cytidine
CMP + uridine
-
-
-
-
?
5'-UMP + guanosine
GMP + uridine
-
-
-
-
?
5'-UMP + inosine
IMP + uridine
-
-
-
-
?
5'-UMP + uridine
UMP + uridine
-
-
-
-
?
acetylphosphate + inosine
acetate + IMP
-
233% of the activity with diphosphate
-
-
?
adenosine 3',5'-bisphosphate + thymidine
adenosine 3'-phosphate + TMP
-
30% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
adenosine 5'-phosphorothionate + H2O
?
-
-
-
-
?
ADP + inosine
AMP + IMP
-
26% of the activity with diphosphate
-
-
?
ADP + thymidine
TMP + AMP
-
50% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
AMP + inosine
adenosine + IMP
-
62% of the activity with diphosphate
-
-
?
AMP + thymidine
adenosine + TMP
ATP + inosine
ADP + IMP
-
13% of the activity with diphosphate
-
-
?
ATP + thymidine
ADP + TMP
-
18% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
beta-glycerophosphate + uridine
glycerol + UMP
-
about 15% of the activity with phenyl phosphate
-
-
?
carbamoyl phosphate + inosine
carbamate + IMP
-
276% of the activity with diphosphate
-
-
?
D-ribose 5-phosphate + thymidine
D-ribose + TMP
-
13% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
D-ribose 5-phosphate + uridine
D-ribose + UMP
-
about 30% of the activity with phenyl phosphate
-
-
?
diphosphate + adenosine
phosphate + 5'-AMP
-
35% of the activity with inosine
-
?
diphosphate + cytidine
phosphate + 5'-AMP
-
29% of the activity with inosine
-
?
diphosphate + guanosine
phosphate + 5'-GMP
-
35% of the activity with inosine
-
?
diphosphate + inosine
phosphate + 5'-IMP
diphosphate + uridine
phosphate + 5'-AMP
-
47% of the activity with inosine
-
?
diphosphate + xanthosine
phosphate + 5'-XMP
-
54% of the activity with inosine
-
?
glucose 6-phosphate + inosine
D-glucose + IMP
-
95% of the activity with diphosphate
-
-
?
p-nitrophenylphosphate + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl)thymine
4-nitrophenol + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl)thymine 5'-phosphate
-
-
-
-
?
p-nitrophenylphosphate + beta-L-2',3'-dideoxycytidine
4-nitrophenol + beta-L-2',3'-dideoxycytidine 5'-phosphate
-
-
-
-
?
p-nitrophenylphosphate + L-3'-deoxy-2',3'-didehydrothymidine
4-nitrophenol + L-3'-deoxy-2',3'-didehydrothymidine 5'-phosphate
-
-
-
-
?
p-nitrophenylphosphate + L-3'-deoxy-3'-chlorothymidine
4-nitrophenol + L-3'-deoxy-3'-chlorothymidine 5'-phosphate
-
-
-
-
?
p-nitrophenylphosphate + L-thymidine
4-nitrophenol + L-thymidine 5'-phosphate
-
-
-
-
?
p-nitrophenylphosphate + O2,2'-anhydro-beta-L-5-methyluridine
4-nitrophenol + O2,2'-anhydro-beta-L-5-methyluridine 5'-phosphate
-
-
-
-
?
phenyl phosphate + adenosine
AMP + phenol
-
-
-
-
?
phenyl phosphate + cytidine
CMP + phenol
-
-
-
-
?
phenyl phosphate + deoxyadenosine
dAMP + phenol
-
-
-
-
?
phenyl phosphate + deoxycytidine
dCMP + phenol
-
-
-
-
?
phenyl phosphate + deoxyguanosine
dGMP + phenol
-
-
-
-
?
phenyl phosphate + deoxyuridine
dUMP + phenol
-
-
-
-
?
phenyl phosphate + guanosine
GMP + phenol
-
-
-
-
?
phenyl phosphate + thymidine
TMP + phenol
-
-
-
-
?
phenyl phosphate + uridine
5'-UMP + phenol
-
-
-
-
?
phenylphosphate + inosine
IMP + phenol
tripolyphosphate + inosine
? + IMP
additional information
?
-
3'-AMP + cytidine
CMP + adenosine
-
-
-
-
?
3'-AMP + cytidine
CMP + adenosine
-
-
-
-
?
3'-AMP + guanosine
GMP + adenosine
-
-
-
-
?
3'-AMP + guanosine
GMP + adenosine
-
-
-
-
?
3'-AMP + uridine
adenosine + UMP
-
70% of the activity with phenyl phosphate
-
-
?
3'-AMP + uridine
adenosine + UMP
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyadenosine
2'-deoxyadenosine 5'-phosphate + 2'-deoxyadenosine 3'-phosphate + 4-nitrophenol
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyadenosine
2'-deoxyadenosine 5'-phosphate + 2'-deoxyadenosine 3'-phosphate + 4-nitrophenol
-
-
5'- and 3'-nucleotide products in the approximate ratio of 2:1
?
4-nitrophenyl phosphate + adenosine
5'-AMP + 4-nitrophenol
-
-
-
-
?
4-nitrophenyl phosphate + adenosine
5'-AMP + 4-nitrophenol
-
-
-
-
?
4-nitrophenyl phosphate + adenosine
5'-AMP + 4-nitrophenol
-
-
-
-
?
4-nitrophenyl phosphate + adenosine
5'-AMP + 4-nitrophenol
-
-
-
?
4-nitrophenyl phosphate + inosine
4-nitrophenol + IMP
-
-
-
-
?
4-nitrophenyl phosphate + inosine
4-nitrophenol + IMP
-
-
-
-
?
4-nitrophenyl phosphate + inosine
4-nitrophenol + IMP
-
-
-
-
?
4-nitrophenyl phosphate + inosine
4-nitrophenol + IMP
-
115% of the activity with diphosphate
-
-
?
4-nitrophenyl phosphate + inosine
4-nitrophenol + IMP
-
115% of the activity with diphosphate
-
-
?
4-nitrophenyl phosphate + thymidine
4-nitrophenol + TMP
-
-
-
-
?
4-nitrophenyl phosphate + thymidine
4-nitrophenol + TMP
-
24% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
4-nitrophenyl phosphate + uridine
4-nitrophenol + 5'-UMP
-
-
-
?
4-nitrophenyl phosphate + uridine
4-nitrophenol + 5'-UMP
-
-
-
-
?
4-nitrophenyl phosphate + uridine
4-nitrophenol + 5'-UMP
-
-
-
-
?
4-nitrophenyl phosphate + uridine
4-nitrophenol + 5'-UMP
-
115% of the activity with diphosphate
-
-
?
4-nitrophenyl phosphate + uridine
4-nitrophenol + 5'-UMP
-
115% of the activity with diphosphate
-
-
?
AMP + thymidine
adenosine + TMP
-
-
-
-
?
AMP + thymidine
adenosine + TMP
-
-
-
-
?
diphosphate + inosine
phosphate + 5'-IMP
-
-
-
?
diphosphate + inosine
phosphate + 5'-IMP
-
-
-
?
phenylphosphate + inosine
IMP + phenol
-
164% of the activity with diphosphate
-
-
?
phenylphosphate + inosine
IMP + phenol
-
164% of the activity with diphosphate
-
-
?
tripolyphosphate + inosine
? + IMP
-
13% of the activity with diphosphate
-
-
?
tripolyphosphate + inosine
? + IMP
-
13% of the activity with diphosphate
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
the enzyme also exhibits phosphatase activity
-
-
?
additional information
?
-
-
protein carries both transfer and hydrolytic functions
-
-
?
additional information
?
-
-
protein carries both transfer and hydrolytic functions
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
no activity with L-adenosine, L-cytidine, L-ribofuranosylthymine, and 1-(beta-L-arabinofuranosyl)thymine
-
-
?
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Kit, S.; Leung, W.C.; Trkula, D.; Dubbs, D.R.
Characterization of nucleoside phosphotransferase and thymidine kinase activities of chick embryo cells and of chick-mouse somatic cell hybrids
Arch. Biochem. Biophys.
169
66-76
1975
Gallus gallus
brenda
Brunngraber, E.F.; Chargaff, E.
Purification and properties of a nucleoside phosphotransferase from carrot
J. Biol. Chem.
242
4834-4840
1967
Daucus carota
brenda
Brunngraber, E.F.
Nucleoside phosphotransferase from carrot
Methods Enzymol.
51
387-394
1978
Daucus carota
brenda
Rodgers, R.; Chargaff, E.
Nucleoside phosphotransferase from carrot. Chemical characterization and investigation of catalytic sites
J. Biol. Chem.
247
5448-5455
1972
Daucus carota
brenda
Chao, H.M.
Nucleoside phosphotransferase from Erwinia herbicola, a new membrane-bound enzyme
J. Biol. Chem.
251
2330-2333
1976
Pantoea agglomerans
brenda
Prasher, D.C.; Carr, M.C.; Ives, D.H.; Tsai, T.C.; Frey, P.A.
Nucleoside phosphotransferase from barley. Characterization and evidence for ping pong kinetics involving phosphoryl enzyme
J. Biol. Chem.
257
4931-4939
1982
Hordeum vulgare
brenda
Billich, A.; Stockhowe, U.; Witzel, H.
Nucleoside phosphotransferase from malt sprouts. I. Isolation, characterization and specificity of the enzyme
Biol. Chem. Hoppe-Seyler
367
267-278
1986
Hordeum vulgare
brenda
Billich, A.; Witzel, H.
Nucleoside phosphotransferase from malt sprouts. III. Studies on metal ion requirement, solvent effects, kinetics and reaction mechanism
Biol. Chem. Hoppe-Seyler
367
291-300
1986
Hordeum vulgare
brenda
Yuyama, S.; Corff, S.; Young, P.G.
Identification of true thymidine kinase in Tetrahymena pyriformis ST
Arch. Biochem. Biophys.
188
64-69
1978
Tetrahymena pyriformis, Tetrahymena pyriformis ST
brenda
Tesoriere, G.; Vento, R.; Calvaruso, G.; Taibi, G.; Gueli, M.C.
Kinetic properties of a nucleoside phosphotransferase of chick embryo
Biochim. Biophys. Acta
660
348-358
1981
Gallus gallus
brenda
Tesoriere, G.; Vento, R.; Tesoriere, L.; Giuliano, M.
The purification and properties of nucleoside phosphotransferase from mucosa of chicken intestine
Biochim. Biophys. Acta
786
231-244
1984
Gallus gallus
brenda
Guranowski, A.
Nucleoside phosphotransferase from yellow lupin seedling cotyledons
Biochim. Biophys. Acta
569
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