Information on EC 2.7.1.77 - nucleoside phosphotransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.1.77
-
RECOMMENDED NAME
GeneOntology No.
nucleoside phosphotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a nucleotide + a 2'-deoxynucleoside = a nucleoside + a 2'-deoxynucleoside 5'-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
nucleotide:nucleoside 5'-phosphotransferase
Phenyl phosphate and nucleoside 3'-phosphates can act as donors, although not so well as nucleoside 5'-phosphates. Nucleosides as well as 2'-deoxyribonucleosides can act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
9055-37-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
NCIMB10466
-
-
Manually annotated by BRENDA team
NCIMB10466
-
-
Manually annotated by BRENDA team
strain 47/3
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2',3')-uridylic acid + uridine
uridine + UMP
show the reaction diagram
-
about 10% of the activity with phenyl phosphate
-
-
?
3'-AMP + adenosine
AMP + adenosine
show the reaction diagram
-
-
-
-
?
3'-AMP + cytidine
CMP + adenosine
show the reaction diagram
-
-
-
-
?
3'-AMP + guanosine
GMP + adenosine
show the reaction diagram
-
-
-
-
?
3'-AMP + inosine
IMP + adenosine
show the reaction diagram
-
-
-
-
?
3'-AMP + uridine
adenosine + UMP
show the reaction diagram
-
70% of the activity with phenyl phosphate
-
-
?
3'-AMP + uridine
UMP + adenosine
show the reaction diagram
-
-
-
-
?
3'-CMP + thymidine
cytosine + TMP
show the reaction diagram
-
20% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
3'-dAMP + thymidine
TMP + 2'-deoxyadenosine
show the reaction diagram
-
56% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
3'-TMP + thymidine
thymidine + TMP
show the reaction diagram
-
19% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
4-nitrophenyl phosphate + 1-(beta-D-ribofuranosyl)-2(1H)pyridone
4-nitrophenol + 1-(beta-D-ribofuranosyl)-2(1H)pyridone 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 1-(beta-D-ribofuranosyl)-4(1H)pyridone
4-nitrophenol + 1-(beta-D-ribofuranosyl)-4(1H)pyridone 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 2'-bromo-2'-deoxyuridine
4-nitrophenol + 2'-bromo-2'-deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxy-2'-methyluridine
4-nitrophenol + 2'-deoxy-2'-methyluridine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyadenosine
4-nitrophenol + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
4-nitrophenyl phosphate + 2'-deoxyadenosine
5'-dAMP + 3'-dAMP + nitrophenol
show the reaction diagram
4-nitrophenyl phosphate + 2'-deoxycytidine
4-nitrophenol + 2'-deoxycytidine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyguanosine
4-nitrophenol + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 2'-deoxyinosine
4-nitrophenol + 2'-deoxyinosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 2'-O-methylcytidine
4-nitrophenol + 2'-O-methylcytidine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 2-thiouridine
4-nitrophenol + 2-thiouridine 5'phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxy-3'-methyluridine
4-nitrophenol + 3'-deoxy-3'-methyluridine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxyadenosine
4-nitrophenol + 3'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxyadenosine
4-nitrophenol + 3'-deoxyadenosine 5'phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 3'-deoxyinosine
4-nitrophenol + 3'-deoxyinosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 3'-fluoroinosine
4-nitrophenol + 3'-fluoro-IMP
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 3'-O-methylcytidine
4-nitrophenol + 3'-O-methylcytidine 5'phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 3-(beta-D-ribofuranosyl)uric acid
4-nitrophenol + 3-(beta-D-ribofuranosyl)uric acid 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 5-bromouridine
4-nitrophenol + 5-bromouridine 5'phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 5-chlorouridine
4-nitrophenol + 5-chlorouridine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 5-hydroxyuridine
4-nitrophenol + 5-hydroxyuridine 5'phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 5-iodouridine
4-nitrophenol + 5-iodouridine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 6-chloro-9-(beta-D-ribofuranosyl)purine
4-nitrophenol + 6-chloro-9-(beta-D-ribofuranosyl)purine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 7-methylguanosine
4-nitrophenol + 7-methylguanosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + 9-(beta-D-ribofuranosyl)purine
4-nitrophenol + 9-(beta-D-ribofuranosyl)purine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + adenosine
4-nitrophenol + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + adenosine
5'-AMP + 4-nitrophenol
show the reaction diagram
4-nitrophenyl phosphate + beta-L-2',3'-dideoxycytidine
4-nitrophenol + beta-L-2',3'-dideoxycytidine 5'-phosphate
show the reaction diagram
-
38% maximum conversion to 5'-monophosphate
-
-
?
4-nitrophenyl phosphate + beta-pseudouridine
4-nitrophenol + beta-pseudouridine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + cytidine
4-nitrophenol + CMP
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + deazauridine
4-nitrophenol + deazauridine 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + formycin B
4-nitrophenol + ?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + guanosine
4-nitrophenol + GMP
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + inosine
4-nitrophenol + IMP
show the reaction diagram
4-nitrophenyl phosphate + thymidine
4-nitrophenol + TMP
show the reaction diagram
4-nitrophenyl phosphate + thymidine 3'-[(4-nitrophenyl)-phosphate]
4-nitrophenol + thymidine 3'-[(4-nitrophenyl)-phosphate] 5'-phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + uridine
4-nitrophenol + 5'-UMP
show the reaction diagram
4-nitrophenyl phosphate + xanthosine
4-nitrophenol + XMP
show the reaction diagram
-
-
-
-
?
5'-adenylic acid + uridine
adenosine + UMP
show the reaction diagram
-
70% of the activity with phenyl phosphate
-
-
?
5'-AMP + adenosine
AMP + adenosine
show the reaction diagram
-
-
-
-
?
5'-AMP + cytidine
CMP + adenosine
show the reaction diagram
-
-
-
-
?
5'-AMP + guanosine
GMP + adenosine
show the reaction diagram
-
-
-
-
?
5'-AMP + inosine
IMP + adenosine
show the reaction diagram
-
-
-
-
?
5'-AMP + thymidine
adenosine + TMP
show the reaction diagram
-
72% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
5'-AMP + uridine
UMP + adenosine
show the reaction diagram
-
-
-
-
?
5'-CMP + adenosine
AMP + cytidine
show the reaction diagram
-
-
-
-
?
5'-CMP + cytidine
CMP + cytidine
show the reaction diagram
-
-
-
-
?
5'-CMP + guanosine
GMP + cytidine
show the reaction diagram
-
-
-
-
?
5'-CMP + inosine
IMP + cytidine
show the reaction diagram
-
-
-
-
?
5'-CMP + thymidine
cytidine + TMP
show the reaction diagram
-
92% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
5'-CMP + uridine
UMP + cytidine
show the reaction diagram
-
-
-
-
?
5'-dAMP + thymidine
5'-deoxyadenosine + TMP
show the reaction diagram
-
-
-
-
?
5'-dCMP + thymidine
2'-deoxycytidine + TMP
show the reaction diagram
-
46% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
5'-dGMP + thymidine
TMP + 2'-deoxyguanosine
show the reaction diagram
-
65% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
5'-dTMP + thymidine
5'-deoxythymidine + TMP
show the reaction diagram
-
-
-
-
?
5'-GMP + adenosine
AMP + guanosine
show the reaction diagram
-
-
-
-
?
5'-GMP + cytidine
CMP + guanosine
show the reaction diagram
-
-
-
-
?
5'-GMP + inosine
IMP + guanosine
show the reaction diagram
-
-
-
-
?
5'-GMP + thymidine
TMP + guanosine
show the reaction diagram
-
61% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
5'-GMP + uridine
UMP + guanosine
show the reaction diagram
-
-
-
-
?
5'-IMP + adenosine
AMP + inosine
show the reaction diagram
-
-
-
-
?
5'-IMP + cytidine
CMP + inosine
show the reaction diagram
-
-
-
-
?
5'-IMP + guanosine
GMP + inosine
show the reaction diagram
-
-
-
-
?
5'-IMP + inosine
IMP + inosine
show the reaction diagram
-
-
-
-
?
5'-IMP + uridine
UMP + inosine
show the reaction diagram
-
-
-
-
?
5'-UMP + adenosine
AMP + uridine
show the reaction diagram
-
-
-
-
?
5'-UMP + cytidine
CMP + uridine
show the reaction diagram
-
-
-
-
?
5'-UMP + guanosine
GMP + uridine
show the reaction diagram
-
-
-
-
?
5'-UMP + inosine
IMP + uridine
show the reaction diagram
-
-
-
-
?
5'-UMP + uridine
UMP + uridine
show the reaction diagram
-
-
-
-
?
acetylphosphate + inosine
acetate + IMP
show the reaction diagram
-
233% of the activity with diphosphate
-
-
?
adenosine 3',5'-bisphosphate + thymidine
adenosine 3'-phosphate + TMP
show the reaction diagram
-
30% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
adenosine 5'-phosphorothionate + H2O
?
show the reaction diagram
-
-
-
-
?
ADP + inosine
AMP + IMP
show the reaction diagram
-
26% of the activity with diphosphate
-
-
?
ADP + thymidine
TMP + AMP
show the reaction diagram
-
50% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
AMP + inosine
adenosine + IMP
show the reaction diagram
-
62% of the activity with diphosphate
-
-
?
AMP + thymidine
adenosine + TMP
show the reaction diagram
ATP + inosine
ADP + IMP
show the reaction diagram
-
13% of the activity with diphosphate
-
-
?
ATP + thymidine
ADP + TMP
show the reaction diagram
-
18% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
beta-glycerophosphate + uridine
glycerol + UMP
show the reaction diagram
-
about 15% of the activity with phenyl phosphate
-
-
?
carbamoyl phosphate + inosine
carbamate + IMP
show the reaction diagram
-
276% of the activity with diphosphate
-
-
?
D-ribose 5-phosphate + thymidine
D-ribose + TMP
show the reaction diagram
-
13% of the activity with 5'-dAMP or 5'-dTMP
-
-
?
D-ribose 5-phosphate + uridine
D-ribose + UMP
show the reaction diagram
-
about 30% of the activity with phenyl phosphate
-
-
?
diphosphate + adenosine
phosphate + 5'-AMP
show the reaction diagram
-
35% of the activity with inosine
-
?
diphosphate + cytidine
phosphate + 5'-AMP
show the reaction diagram
-
29% of the activity with inosine
-
?
diphosphate + guanosine
phosphate + 5'-GMP
show the reaction diagram
-
35% of the activity with inosine
-
?
diphosphate + inosine
phosphate + 5'-IMP
show the reaction diagram
diphosphate + uridine
phosphate + 5'-AMP
show the reaction diagram
-
47% of the activity with inosine
-
?
diphosphate + xanthosine
phosphate + 5'-XMP
show the reaction diagram
-
54% of the activity with inosine
-
?
glucose 6-phosphate + inosine
D-glucose + IMP
show the reaction diagram
-
95% of the activity with diphosphate
-
-
?
p-nitrophenylphosphate + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl)thymine
4-nitrophenol + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl)thymine 5'-phosphate
show the reaction diagram
p-nitrophenylphosphate + beta-L-2',3'-dideoxycytidine
4-nitrophenol + beta-L-2',3'-dideoxycytidine 5'-phosphate
show the reaction diagram
p-nitrophenylphosphate + L-3'-deoxy-2',3'-didehydrothymidine
4-nitrophenol + L-3'-deoxy-2',3'-didehydrothymidine 5'-phosphate
show the reaction diagram
p-nitrophenylphosphate + L-3'-deoxy-3'-chlorothymidine
4-nitrophenol + L-3'-deoxy-3'-chlorothymidine 5'-phosphate
show the reaction diagram
p-nitrophenylphosphate + L-thymidine
4-nitrophenol + L-thymidine 5'-phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenylphosphate + O2,2'-anhydro-beta-L-5-methyluridine
4-nitrophenol + O2,2'-anhydro-beta-L-5-methyluridine 5'-phosphate
show the reaction diagram
-
-
-
-
?
phenyl phosphate + adenosine
AMP + phenol
show the reaction diagram
-
-
-
-
?
phenyl phosphate + cytidine
CMP + phenol
show the reaction diagram
-
-
-
-
?
phenyl phosphate + deoxyadenosine
dAMP + phenol
show the reaction diagram
-
-
-
-
?
phenyl phosphate + deoxycytidine
dCMP + phenol
show the reaction diagram
-
-
-
-
?
phenyl phosphate + deoxyguanosine
dGMP + phenol
show the reaction diagram
-
-
-
-
?
phenyl phosphate + deoxyuridine
dUMP + phenol
show the reaction diagram
-
-
-
-
?
phenyl phosphate + guanosine
GMP + phenol
show the reaction diagram
-
-
-
-
?
phenyl phosphate + thymidine
TMP + phenol
show the reaction diagram
-
-
-
-
?
phenyl phosphate + uridine
5'-UMP + phenol
show the reaction diagram
-
-
-
-
?
phenylphosphate + inosine
IMP + phenol
show the reaction diagram
S: 4-nitrophenyl phosphate + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl) thymine
4-nitrophenol + 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl) thymine 5-phosphate
show the reaction diagram
-
80% maximum conversion to 5'-monophosphate
-
-
?
S: 4-nitrophenyl phosphate + beta-L-3'-deoxy-2',3'-didehydrothymidine
4-nitrophenol + beta-L-3'-deoxy-2',3'-didehydrothymidine 5'-phosphate
show the reaction diagram
-
60% maximum conversion to 5'-monophosphate
-
-
?
S: 4-nitrophenyl phosphate + beta-L-3'-deoxy-3'-chlorothymidine
4-nitrophenol + beta-L-3'-deoxy-3'-chlorothymidine 5'-phosphate
show the reaction diagram
-
90% maximum conversion to 5'-monophosphate
-
-
?
S: 4-nitrophenyl phosphate + beta-L-thymidine
4-nitrophenol + beta-L-thymidine 5'-phosphate
show the reaction diagram
-
35% maximum conversion to 5'-monophosphate
-
-
?
S: 4-nitrophenyl phosphate + O2,2'-anhydro-beta-L-5-methyluridine
4-nitrophenol + O2,2'-anhydro-beta-L-5-methyluridine 5'-phosphate
show the reaction diagram
-
65% maximum conversion to 5'-monophosphate
-
-
?
tripolyphosphate + inosine
? + IMP
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Fe2+
-
can restore activity of the metal-free apoenzyme to a minor extent
Mn2+
-
Mg2+, Mn2+ or Co2+ required
Ni2+
-
can restore activity of the metal-free apoenzyme to a minor extent
Zn2+
-
can restore activity of the metal-free apoenzyme to a minor extent
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4-Dinitrofluorobenzene
-
-
AgCl
-
1 mM, 91% inhibition
arsenate
-
strong competitive inhibitor
cAMP
-
strong competitive inhibitor
Cd2+
-
2 mM, at least 80% inhibition of transphosphorylation
CuSO4
-
1 mM, 23% inhibition
cysteine
glutathione
iodoacetate
-
1 mM, 41% inhibition
NaF
-
0.25 M, phosphatase is inhibited by less than 10%
PCMB
-
1 mM, 17% inhibition
phosphate
-
competitive inhibitor of the associated enzyme form
Urea
-
6 M, complete inactivation
uridine
-
inhibitor of phosphatase reaction
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetone
-
25%, 2.45fold stimulation
acetonitrile
-
8%, 2.4fold stimulation
Dimethyl formamide
-
5%, 1.8fold, stimulation
dimethyl sulfoxide
-
20%, 1.8fold stimulation
glycerol
-
60%, 1.7fold stimulation
methanol
-
30%, 2.3fold stimulation
NaF
-
0.25 M, enhances activity by about 45%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.44
1-naphthyl phosphate
-
-
0.0059
2'-deoxyadenosine
-
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
0.0153
2'-deoxycytidine
-
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
0.0063
2'-deoxyguanosine
-
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
0.0057
2'-deoxyinosine
-
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
0.26
2-naphthyl phosphate
-
pH 5.5
0.023
3'-AMP
-
pH 5.5
0.0071
3'-deoxyadenosine
-
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
0.0084
3'-deoxyinosine
-
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
0.0066
3'-fluoroinosine
-
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
0.092
3'-UMP
-
pH 5.5
0.28
4-nitrophenyl phosphate
-
pH 5.5
0.018 - 0.4
5'-AMP
0.09
5'-UMP
-
pH 5.5
0.0061
adenosine
-
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
0.22
adenosine 5'-phosphorothionate
-
pH 5.5
0.0169 - 6.39
cytidine
0.087
deoxyadenosine
-
pH 5.0, reaction with AMP
4.15
deoxycytidine
-
pH 5.0, reaction with AMP
0.412
deoxyguanosine
-
pH 5.0, reaction with AMP
0.89
deoxythymidine
-
pH 5.0, reaction with AMP
0.73
diphosphate
-
pH 5.0
0.0064 - 2.13
guanosine
0.0059 - 122
Inosine
3.5
Phenylphosphate
-
phosphotransferase reaction with uridine
0.014
thymidine
-
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
0.0167 - 3.93
uridine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.6
1-naphthyl phosphate
Hordeum vulgare
-
pH 5.5, phosphohydrolase activity
30
2-naphthyl phosphate
Hordeum vulgare
-
pH 5.5, phosphohydrolase activity
6.8
3'-AMP
Hordeum vulgare
-
pH 5.5, phosphohydrolase activity
12
3'-UMP
Hordeum vulgare
-
pH 5.5
77
4-nitrophenyl phosphate
Hordeum vulgare
-
pH 5.5, phosphohydrolase activity
41
5'-AMP
Hordeum vulgare
-
pH 5.5, phosphohydrolase activity
67
5'-UMP
Hordeum vulgare
-
pH 5.5, phosphohydrolase activity
0.4
adenosine-5'-phosphorothionate
Hordeum vulgare
-
pH 5.5, phosphohydrolase activity
additional information
additional information
Hordeum vulgare
-
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
inhibitors of phosphohydrolase activity
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0021
-
-
0.23
-
substrate beta-L-thymidine, pH 4.5, 37°C
0.4
-
substrate beta-L-2',3'-dideoxycytidine, pH 4.5, 37°C
0.55
-
substrate adenosine, pH 4.5, 37°C
0.57
-
substrate beta-L-3'-deoxy-2',3'-didehydrothymidine, pH 4.5, 37°C
0.63
-
substrate O2,2'-anhydro-beta-L-5-methyluridine, pH 4.5, 37°C
0.73
-
substrate 1-(2,3-dideoxy-2-fluoro-beta-L-erythro-pentofuranosyl) thymine, pH 4.5, 37°C
0.95
-
substrate beta-L-3'-deoxy-3'-chlorothymidine, pH 4.5, 37°C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
-
phosphotransferase activity
6
-
dephosphorylation
6.5
-
transferase activity
8.8
-
associated and dissociated enzyme form
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 7
-
pH 4.0: about 25% of maximal activity in the reaction with phenyl phosphate and uridine, about 40% of maximal activity with phenyl phosphate and deoxycytidine , pH 7.0: about 20% of maximal activity in the reaction with phenyl phosphate and uridine, about 50% of maximal activity with phenyl phosphate and deoxycytidine
4 - 6.5
-
pH 4.0: 30% of maximal activity, pH 6.5: about 60% of maximal activity, phosphotransferase activity
4.5 - 8.5
-
pH 4.5: about 65% of maximal activity, pH 8.5: about 50% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6 - 4.7
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
-
6 * 25000, SDS-PAGE
26300
-
x * 26300, SDS-PAGE
40000
-
gel filtration
44000
-
gel filtration, equilibrium sedimentation, sucrose density gradient centrifugation
50000
-
2 * 50000, SDS-PAGE
53400
-
non-denaturing PAGE
55000
-
gel filtration
68000
-
sucrose density gradient sedimentation
72000
-
gel filtration
160000
-
gel filtration
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 26300, SDS-PAGE
hexamer
monomer
-
1 * 72000, SDS-PAGE
additional information
-
multisubunit protein at different degrees of association, it can dissociate into a component with a marked fall in catalytic activity
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 12
-
stable
642002
9.5
-
without protecting agent, about one-third of the initial transfer activity is retained whereas the hydrolase function is almost entirely lost after 22 h
641991, 641992
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
pH 5.0, 30 min, stable
50
-
5 min, stable
63
-
pH 8.0, 3 min, 50 mM Tris-HCl buffer, 50% loss of activity
68
-
pH 8.0, 3 min, 50 mM Tris-HCl buffer containing 10% glycerol, 50% loss of activity
72
-
pH 8.0, 3 min, 50 mM potassium phosphate buffer containing 10% glycerol, pH 6.8, 50% loss of activity
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis first against EDTA or oxime and then against water produces an inhibition which results in the recovery of 36% of the initial phosphotransferase activity and 65% of the initial phosphatase activity
-
does not lose acttivity on lyophilization
-
no loss of activity after dialysis overnight against distilled water
-
not stable to SDS
-
stable in presence of 2 M urea, but not 4 M urea
-
storage in 20% acetone for 1 week without loss of activity
-
the enzyme can be isolated and handled only in the presence of detergents
-
the enzyme tolerates repeated freeze-thawing
-
tolerates repeated freezing and thawing
-
withstands repeated freezing and thawing if the protein concentration is less than 0.5 mg/ml
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for at least 1 months
-
-20°C, stable for at least 6 months
-
0°C, 0.01% Triton X-100 or 0.25 M sodium acetate, pH 5, 44 h, phosphotransferase activity decreases 20% and 50% respectively
-
4°C, 1 week, 80% of the transferase activity is retained, no loss of hydrolase activity
-
4°C, 10% glycerol, pH 7.4, stable for at least 2 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
phenyl Sepharose CL-4B column chromatography
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
method for transformation of nucleosides into 5’-monophosphates based on the shift in the equilibrium state of the reaction. Extent of nucleoside transformation is 41-91%