Information on EC 2.7.1.73 - inosine kinase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota

EC NUMBER
COMMENTARY hide
2.7.1.73
-
RECOMMENDED NAME
GeneOntology No.
inosine kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + inosine = ADP + IMP
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
adenine and adenosine salvage V
-
-
guanine and guanosine salvage III
-
-
Purine metabolism
-
-
purine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:inosine 5'-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
37237-46-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
-
-
-
?
ATP + guanosine
ADP + GMP
show the reaction diagram
ATP + inosine
ADP + 5'-IMP
show the reaction diagram
ATP + inosine
ADP + IMP
show the reaction diagram
ATP + xanthosine
ADP + XMP
show the reaction diagram
-
-
-
-
?
CTP + inosine
CDP + IMP
show the reaction diagram
-
-
-
-
?
dATP + guanosine
dADP + GMP
show the reaction diagram
-
-
-
?
dATP + inosine
dADP + IMP
show the reaction diagram
UTP + guanosine
UDP + GMP
show the reaction diagram
UTP shows 20% of the activity with ATP
-
-
?
UTP + inosine
UDP + IMP
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + guanosine
ADP + GMP
show the reaction diagram
ATP + inosine
ADP + IMP
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
guanosine
-
inhibits phosphorylation of inosine
Inosine
-
inhibits phosphorylation of guanosine
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mn2+
-
can partially replace Mg2+ in activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.51 - 0.71
ATP
0.66 - 2.4
dATP
0.0061 - 0.014
guanosine
0.07 - 2.1
Inosine
1.45
MgATP2-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
substrate inosine, crude cell extract, pH 7.5, temperature not specified in the publication
60
-
substrate guanosine, crude cell extract, pH 7.5, temperature not specified in the publication
120
-
substrate guanosine, crude cell extract, pH 7.5, temperature not specified in the publication; substrate inosine, crude cell extract, pH 7.5, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9
inosine kinase
8.2
guanosine kinase
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10.5
-
pH 6.0: about 40% of maximal activity, pH 10.5: about 85% of maximal activity
6.5 - 8.2
-
pH 6.5: about 60% of maximal activity, pH 8.2: about 70% of maximal activity, Tris-maleate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26 - 39
inosine kinase
38
guanosine kinase
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
intermembrane space
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
-
2 * 45000
48400
x * 48400, calculation from nucleotide sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 48400, calculation from nucleotide sequence
dimer
-
2 * 45000
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
rapid loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
very low stability
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene gsk, recombinant expression in Corynebacterium ammoniagenes ATP-regenerating strain ATCC 21477, the Escherichia coli trp promoter proves to be most efficient with regard to inducing the expression of gsk, as compared to tac promoter
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gene gsk, several metabolic enzymes such as GMP synthetase and IMP dehydrogenase, GMP reductase, and guanosine-inosine kinase, all involved in the biosynthesis of GMP, are co-overexpressed in recombinant Escherichia coli TOP10 and BL21star(DE3) cells becoming able to produce GDP-L-fucose, pH-stat fed-batch fermentations quantification and optimization, overview
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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overexpression of Gsk in the fed-batch fermentation in recombinant Escherichia coli strain BL21star(DE3) with glucose as the sole carbon source, leads to a significant improvement of GDP-L-fucose production, profiles of cell growth and GDP-L-fucose production, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nutrition
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practical possibility of producing 5'-GMP by phosphorylation of guanosine using a guanosine-inosine kinase coupled with ATP regeneration
synthesis
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the recombinant strain, which expresses gene gsk and has both inosine kinase activity and ATP-regenerating activity, is used to induce the phosphorylation of inosine to produce inosine 5'-monophosphate, which is widely used as a flavor enhancer
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