Information on EC 2.7.1.72 - streptomycin 6-kinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.72
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RECOMMENDED NAME
GeneOntology No.
streptomycin 6-kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + streptomycin = ADP + streptomycin 6-phosphate
show the reaction diagram
dATP can replace ATP, dihydrostreptomycin, streptidine and 2-deoxystreptidine can act as acceptors
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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streptomycin biosynthesis
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Streptomycin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
ATP:streptomycin 6-phosphotransferase
dATP can replace ATP; and dihydrostreptomycin, streptidine and 2-deoxystreptidine can act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
37278-11-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
strain ATCC 14607
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-
Manually annotated by BRENDA team
strain HUT 6037
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Manually annotated by BRENDA team
strain HUT 6037
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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the enzyme confers resistance to streptomycin
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-deoxydihydrostreptomycin + ATP
ADP + 3'-deoxydihydrostreptomycin 6-phosphate
show the reaction diagram
-
-
-
-
?
ATP + dihydrostreptomycin
ADP + dihydrostreptomycin 6-phosphate
show the reaction diagram
ATP + streptomycin
ADP + streptomycin 6-phosphate
show the reaction diagram
dATP + streptomycin
dADP + streptomycin 6-phosphate
show the reaction diagram
-
-
-
-
?
dihydrostreptomycin 3''-alpha-phosphate + ATP
ADP + dihydrostreptomycin 3''-alpha-phosphate 6-phosphate
show the reaction diagram
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-
-
-
?
dihydrostreptomycin 3''-phosphate + ATP
ADP + dihydrostreptomycin 3'',6-bisphosphate
show the reaction diagram
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-
-
-
?
streptidine + ATP
streptidine 6-phosphate + ADP
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + streptomycin
ADP + streptomycin 6-phosphate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
10 mM, rather less stimulatory than Mg2+
Cu2+
-
10 mM, rather less stimulatory than Mg2+
Fe2+
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10 mM, slight stimulation
Zn2+
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10 mM, slight stimulation
additional information
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not affected by Ca2+, 0.2-100 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine
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competitive
ADP
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noncompetitive
AgNO3
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strong inhibition
AMP
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noncompetitive
dihydrostreptomycin
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diphosphate
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noncompetitive
EDTA
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strong inhibition
streptomycin
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additional information
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no inhibition by sulfhydryl reagents, formamidine disulfide, p-chloromercuribenzoate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4 - 1.03
ATP
11
dihydrostreptomycin
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28
streptidine
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0.38 - 3.5
streptomycin
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.7
ATP
Escherichia coli
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at pH 7.5 and 25°C
1.7
streptomycin
Escherichia coli
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at pH 7.5 and 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.5
streptomycin
Escherichia coli
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at pH 7.5 and 25°C
2358
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
adenosine
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3
diphosphate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11.35
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purified enzyme
additional information
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assay methods
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
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assay at, method 1
additional information
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pI: 6.6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31400
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gel filtration
36000
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1 * 36000, SDS-PAGE
38000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta(DE3)pLysS cells
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