Information on EC 2.7.1.7 - mannokinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.7
-
RECOMMENDED NAME
GeneOntology No.
mannokinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + D-mannose = ADP + D-mannose 6-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
1,5-anhydrofructose degradation
-
-
CMP-2-keto-3-deoxy-D-glycero-D-galacto-nononate biosynthesis
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mannitol degradation II
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d-mannose degradation
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Fructose and mannose metabolism
-
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Amino sugar and nucleotide sugar metabolism
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Metabolic pathways
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Microbial metabolism in diverse environments
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-
SYSTEMATIC NAME
IUBMB Comments
ATP:D-mannose 6-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9030-54-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain KM
SwissProt
Manually annotated by BRENDA team
strain KM
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
a single enzyme catalyzes phosphorylation of D-fructose and D-mannose
-
-
Manually annotated by BRENDA team
L5-30; wild-type and mannokinase defectice strains
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 2-deoxy-D-glucose
ADP + 2-deoxy-D-glucose 6-phosphate
show the reaction diagram
-
20% of the activity with D-mannose
-
-
?
ATP + 2-deoxy-D-mannose
ADP + 2-deoxy-D-mannose 6-phosphate
show the reaction diagram
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
ATP + D-glucosamine
ADP + D-glucosamine 6-phosphate
show the reaction diagram
-
5% of the activity with D-mannose
-
-
-
ATP + D-glucose
ADP + D-glucose 6-phosphate
show the reaction diagram
ATP + D-mannosamine
ADP + D-mannosamine 6-phosphate
show the reaction diagram
-
15% of the activity with D-mannose
-
-
-
ATP + D-mannose
ADP + D-mannose 6-phosphate
show the reaction diagram
ATP + D-mannose
ADP + mannose 6-phosphate
show the reaction diagram
ATP + mannoheptulose
ADP + mannoheptulose 6-phosphate
show the reaction diagram
CTP + 2-deoxy-D-mannose
CDP + 2-deoxy-D-mannose 6-phosphate
show the reaction diagram
-
-
-
-
?
CTP + D-glucose
CDP + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
CTP + D-mannosamine
CDP + D-mannosamine 6-phosphate
show the reaction diagram
-
-
-
-
?
CTP + D-mannose
CDP + D-mannose 6-phosphate
show the reaction diagram
CTP + mannoheptulose
CDP + mannoheptulose 6-phosphate
show the reaction diagram
-
-
-
-
?
dATP + D-mannose
dADP + D-mannose 6-phosphate
show the reaction diagram
-
-
-
-
?
GTP + 2-deoxy-D-mannose
GDP + 2-deoxy-D-mannose 6-phosphate
show the reaction diagram
-
-
-
-
?
GTP + D-glucose
GDP + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
GTP + D-mannosamine
GDP + D-mannosamine 6-phosphate
show the reaction diagram
-
-
-
-
?
GTP + D-mannose
GDP + D-mannose 6-phosphate
show the reaction diagram
GTP + mannoheptulose
GDP + mannoheptulose 6-phosphate
show the reaction diagram
-
-
-
-
?
hexametaphosphate + D-mannose
pentaphosphate + D-mannose 6-phosphate
show the reaction diagram
ITP + 2-deoxy-D-mannose
IDP + 2-deoxy-D-mannose 6-phosphate
show the reaction diagram
-
-
-
-
?
ITP + D-glucose
IDP + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
ITP + D-mannosamine
IDP + D-mannosamine 6-phosphate
show the reaction diagram
-
-
-
-
?
ITP + D-mannose
IDP + D-mannose 6-phosphate
show the reaction diagram
ITP + mannoheptulose
IDP + mannoheptulose 6-phosphate
show the reaction diagram
-
-
-
-
?
TTP + D-mannose
TDP + D-mannose 6-phosphate
show the reaction diagram
-
30% of the activity with ATP
-
-
?
UTP + D-mannose
UDP + D-mannose 6-phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose
ADP + D-fructose 6-phosphate
show the reaction diagram
-
enzyme initiates the metabolism of D-fructose and D-mannose
-
-
?
ATP + D-mannose
ADP + D-mannose 6-phosphate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
dependent on, can be substituted by polyphosphate, binding site is the same as for polyphosphate binding
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
activates, 11% activity with polyphosphate compared to Mg2+, no activity with ATP
Fe2+
activates, 15% activity with ATP compared to Mg2+, no activity with polyphosphate
Ni2+
-
40% of the activity with Mg2+
Zn2+
activates, 80% and 19% activity with polyphosphate and ATP, respectively, compared to Mg2+
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-deoxy-D-mannose
-
-
alpha-Methyl-D-mannosamine
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-
D-fructose
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competitive to glucose
D-mannoheptulose
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-
D-mannosamine
D-mannose
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competitive to glucose
D-mannose 6-phosphate
L-sorbose
-
competitive
lyxose
MgADP-
Mn2+
-
at a Mn2+/ATP ratio of more than 2
N-acetyl-D-mannosamine
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-
additional information
-
up to 20 mM fructose 6-phosphate shows no inhibitory effect
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Polyphosphate
dependent on, can be substituted by ATP, nonprocessive utilization, binding site is the same as for ATP binding
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15
2-deoxy-D-mannose
-
pH 7.5, 25C
10
2-deoxyglucose
-
pH 7.5, 25C
0.2
ATP
pH 7.0
2
CTP
-
pH 7.5, 25C
0.3 - 0.4
D-fructose
5
D-glucosamine
-
pH 7.5, 25C
0.5 - 10
D-glucose
20
D-mannosamine
-
pH 7.5, 25C
0.05 - 15
D-mannose
0.6
dATP
-
pH 7.5, 25C
0.5
GTP
-
pH 7.5, 25C
0.02
Hexametaphosphate
pH 7.0
0.3
ITP
-
pH 7.5, 25C
0.4
mannoheptulose
-
pH 7.5, 25C
0.2 - 1.1
MgATP2-
4
TTP
-
pH 7.5, 25C
2
UTP
-
pH 7.5, 25C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15
2-deoxy-D-mannose
-
pH 7.5, 25C
30
alpha-Methyl-D-mannosamine
-
pH 7.5, 25C
4
D-glucose
-
pH 7.5, 25C
15
D-Lyxose
-
pH 7.5, 25C
0.4
D-mannoheptulose
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pH 7.5, 25C
0.1 - 20
D-mannosamine
2
L-sorbose
-
pH 7.5, 25C
5
lyxose
-
pH 7.5, 25C
12.5 - 22.5
mannose 6-phosphate
0.1 - 1.1
MgADP-
75
N-acetyl-D-mannosamine
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pH 7.5, 25C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.064
mannokinase activity with polyphosphate in cell extract
0.077
mannokinase activity with ATP in cell extract
110
purified enzyme, mannokinase activity with ATP
220
purified enzyme, mannokinase activity with polyphosphate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9
-
reaction with D-mannose
7 - 8.5
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reaction with D-fructose
additional information
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
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activity falls rapidly outside the range 7-9
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
grown on D-mannose or beta-D-mannan
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000
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sucrose density gradient centrifugation
50000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
5 min, 50% loss of activity, 15 min, complete loss of activity
641894
5 - 10
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25C, 60 min, stable
641894
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
loss of 50% activity after 5 min
57
-
5 min, crude extract stable
61
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5 min, crude extract, 10% loss of activity
64
-
5 min, crude extract, about 80% loss of activity
65
-
5 min, crude extract, complete loss of activity
66
-
5 min, crude extract, complete loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10C, stable for several months
-
0-2C, 1 month, 25% loss of activity
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0C, 0.2 M (NH4)2SO4, pH 7.0, stable for several months
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15C, 8 months, 25% loss of activity
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4C, stable for several days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme 2200fold and 1375fold determined with polyphosphate and ATP, respectively, to homogeneity
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic analysis