Information on EC 2.7.1.66 - undecaprenol kinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.66
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RECOMMENDED NAME
GeneOntology No.
undecaprenol kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + undecaprenol = ADP + undecaprenyl phosphate
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Peptidoglycan biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
ATP:undecaprenol phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9068-22-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
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prokaryotic diacylglycerol kinase and undecaprenol kinase are the lone members of the prokaryotic diacylglycerol kinase and undecaprenol kinase family of multispan membrane enzymes that are very small, lack relationships to any other family of proteins, including water soluble kinases, and that exhibit an unusual structure and active site architecture. The enzymes show complex structure, forming domain-swapped homotrimers in which each monomer subunit has three transmembrane segments. Members of this family are unrelated to the eukaryotic diacylglycerol kinases and their prokaryotic homologs, encoded by the dgkB gene
metabolism
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UDPK activity does not participate in the de novo biosynthetic pathway to undecaprenyl phosphate which does not include free undecaprenol as an intermediate
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (E,E,E)-geranylgeraniol
ADP + (E,E,E)-geranylgeraniyl phosphate
show the reaction diagram
ATP + (Z,E,E)-geranylgeraniol
ADP + (Z,E,E)-geranylgeraniyl phosphate
show the reaction diagram
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5% of the activity with undecaprenol
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-
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ATP + C55-isoprenoid alcohol
ADP + C55-isoprenoid alcohol monophosphate
show the reaction diagram
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-
-
-
?
ATP + dolichol
ADP + dolichyl phosphate
show the reaction diagram
ATP + ficaprenol
ADP + ficaprenyl phosphate
show the reaction diagram
ATP + solanesol
ADP + solanesyl phosphate
show the reaction diagram
ATP + undecaprenol
ADP + undecaprenyl monophosphate
show the reaction diagram
ATP + undecaprenol
ADP + undecaprenyl phosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + undecaprenol
ADP + undecaprenyl phosphate
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
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0.5 M, stimulates about 2-fold
NaF
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1.25 mM stimulates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
guanidine hydrochloride
K2HPO4
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above 10 mM
KCl
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above 10 mM
Mg2+
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above 10 mM
Mn2+
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complete inhibition at 0.01 M
Na2HPO4
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above 10 mM
NaF
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above 5 mM
NH4Cl
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above 10 mM
Urea
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8 M, 20% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Detergents
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overview: activation by detergents, only neutral detergents with short chain and unsaturated chain hydrophobes are successful activators at 25C
ditetradecanoylphosphatidylcholine
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good activator
lysophosphatidylglycerol
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good activator
Phospholipid cofactor
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Triton X-100
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required
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.057 - 2
ATP
0.0055 - 0.014
dolichol
0.5
ficaprenol
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pH 8.2, 37C
0.008 - 0.014
undecaprenol
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2 - 8.8
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pH 7.2: about 80% of maximal activity, pH 8.8: about 70% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17000
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apoprotein, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
lipoprotein
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme is quite stable to SDS, recovered from SDS gel slices after solubilization in 1% SDS, 1% 2-mercaptoethanol
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relatively unstable in suspension in water
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remarkably stable towards SDS, 8 M urea and SH-reagents
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1-butanol
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highly stable
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme
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partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
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the enzyme is a possible anti-cariogenic drug target
synthesis
one-pot reaction leading to undecaprenyl diphosphate disaccharide and starting from undecaprenol, ATP, and the UDP-sugars using the kinase, PglC and PglA