Information on EC 2.7.1.62 - phosphoramidate-hexose phosphotransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.7.1.62
-
RECOMMENDED NAME
GeneOntology No.
phosphoramidate-hexose phosphotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
phosphoramidate + D-hexose = NH3 + alpha-D-hexose 1-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphoramidate:D-hexose 1-phosphotransferase
Activity is observed with several hexoses; of these glucose is the best substrate and the product from it is alpha-D-glucose 1-phosphate. The phosphoramidate donor can be replaced by N-phosphoglycine and by an N-phosphohistidine. May be identical with EC 3.1.3.9 glucose-6-phosphatase.
CAS REGISTRY NUMBER
COMMENTARY hide
9031-45-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Crookes strain, ATCC 8739
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phosphoramidate + 2-deoxy-D-glucose
2-deoxy-D-glucose 1-phosphate + NH3
show the reaction diagram
-
-
-
-
?
phosphoramidate + 3-O-methylglucose
3-O-methylglucose 1-phosphate + NH3
show the reaction diagram
-
-
-
-
?
phosphoramidate + D-glucose
D-glucose 1-phosphate + NH3
show the reaction diagram
phosphoramidate + fructose
fructose 1-phosphate + NH3
show the reaction diagram
-
-
-
?
phosphoramidate + glucosamine
glucosamine 1-phosphate + NH3
show the reaction diagram
-
-
-
-
?
phosphoramidate + mannose
mannose 1-phosphate + NH3
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphoramidate + D-glucose
D-glucose 1-phosphate + NH3
show the reaction diagram
-
phosphoramidate, i.e. PNH2, is delivered by KO3PNH3
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
F-
-
1.5 mM, at pH 8.7 8% inhibition, at pH 6.5 90% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12.5
glucose
-
37°C, pH 8.7
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22.7
-
37°C, pH 8.7
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.7
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
pH 7.5, more than 30 min, no loss of activity
100
-
10 min, about 60% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C or -15°C, 6 years stable
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE