Information on EC 2.7.1.58 - 2-dehydro-3-deoxygalactonokinase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.7.1.58
-
RECOMMENDED NAME
GeneOntology No.
2-dehydro-3-deoxygalactonokinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 2-dehydro-3-deoxy-D-galactonate = ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-galactonate degradation
-
-
Galactose metabolism
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-
L-glucose degradation
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-
Metabolic pathways
-
-
degradation of sugar acids
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Entner Doudoroff pathway
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-
SYSTEMATIC NAME
IUBMB Comments
ATP:2-dehydro-3-deoxy-D-galactonate 6-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
37278-050
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strains NCTC 9001(neotype), B, Crookes, K10, PA309, X289, DF 1070 gnd edd and A1201 kga
-
-
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
subsp. pneumoniae
UniProt
Manually annotated by BRENDA team
subsp. pneumoniae
UniProt
Manually annotated by BRENDA team
Mycobacterium butyricum
-
-
-
Manually annotated by BRENDA team
Ac, (DSM 14322), previously named Pseudomonas sp. strain Ac
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 2-dehydro-3-deoxy-D-galactonate
ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
show the reaction diagram
ATP + 2-dehydro-3-deoxy-D-galactonate
ADP + 2-dehydro-3-deoxy-6-phosphogalactonate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 2-dehydro-3-deoxy-D-galactonate
ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
show the reaction diagram
ATP + 2-dehydro-3-deoxy-D-galactonate
ADP + 2-dehydro-3-deoxy-6-phosphogalactonate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
2-dehydro-3-deoxy-D-galactonate
at pH 8.0 and 42°C
0.3
ATP
at pH 8.0 and 42°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
432
2-dehydro-3-deoxy-D-galactonate
Haloferax volcanii
D4GR05
at pH 8.0 and 42°C
3965
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.04
-
in presence of KDGalA 6-P aldolase
0.086
Mycobacterium butyricum
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-
0.18
-
strain Crookes
0.24
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strain K10
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
Rhizobium meliloti (strain 1021)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31100
el filtration, recombinant detagged enzyme
194000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homohexamer
6 * 35000, SDS-PAGE; 6 * 38000, CTAB-PAGE
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant wild-type and mselenomethionine-labeled enzymes, sitting-drop vapor-diffusion, mixing of 0.0004 ml of 30 mg/m protein in 20 mM HEPES, pH 8.0, 250 mM NaCl, and 2 mM dithiothreitol, with 0.0004 ml of crystallization solution containing 0.1 M magnesium formate, 15%(w/v) PEG 3350, equilibration against 0.135 ml of crystallization solution, at 4°C or 24°C, method optimization, X-ray diffraction structure determination and analysis at 2.1 A resolution
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 50 mM imidazole-HCl, 0.5 mM DTT, 0.6 mM FeSO4 buffer, pH 7.0, remains stable after 4 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and gel filtration
partial
recombinant N-terminally maltose-binding protein fused , His6 -tagged enzyme from Escherichia coli strain BL21 (DE3) by amylose and nickel affinity chromatographies interveined by the cleavage of the tags, to homogeneity, followed by ultrafiltration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli strain BL21 (DE3) from plasmid pRK1037 harboring a fusion protein construct containing an N-terminal maltose-binding protein followed by a tobacco vein mottling virus protease cleavage site, a His6 -tag, a TEV protease cleavage site and the target protein 2-oxo-3-deoxygalactonate kinase
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