Information on EC 2.7.1.47 - D-ribulokinase

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The expected taxonomic range for this enzyme is: Klebsiella aerogenes

EC NUMBER
COMMENTARY hide
2.7.1.47
-
RECOMMENDED NAME
GeneOntology No.
D-ribulokinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + D-ribulose = ADP + D-ribulose 5-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-arabinose degradation II
-
-
Metabolic pathways
-
-
Pentose and glucuronate interconversions
-
-
ribitol degradation
-
-
degradation of pentoses
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:D-ribulose 5-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9026-40-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-arabitol
?
show the reaction diagram
-
poor substrate, not L-isomer
-
-
?
ATP + D-ribulose
?
show the reaction diagram
-
inducible enzyme of ribitol catabolism
-
-
?
ATP + D-ribulose
ADP + D-ribulose 5-phosphate
show the reaction diagram
ATP + ribitol
?
show the reaction diagram
-
poor substrate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-ribulose
?
show the reaction diagram
-
inducible enzyme of ribitol catabolism
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activation, can replace Mg2+ with 23% efficiency
Mn2+
-
activation, can replace Mg2+ with 54% efficiency
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP
-
product inhibition
Mg2+
-
inhibition at a Mg/ATP ratio above 2, 11% inhibition at a ratio of 3
additional information
-
no inhibition by D-ribulose 5-phosphate
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.83
ATP
-
pH 7.7, 27°C
140
D-arabitol
-
pH 7.8, 28°C
0.4 - 0.92
D-ribulose
220
ribitol
-
pH 7.8, 28°C
additional information
additional information
-
kinetic study at 28°C
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
-
broad
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10.5
-
pH 6.0: about 70% of maximal activity, pH 10.5: about half-maximal activity at pH 10.5
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
59000
-
2 * 59000, SDS-PAGE
60000
-
2 * 60000, recombinant enzyme expressed in Escherichia coli strain NC629, SDS-PAGE
112000
-
recombinant enzyme expressed in E. coli strain NC629, gel filtration
116000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
D-ribulose stabilizes
-
EDTA or 2-mercaptoethanol stabilizes during purification
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, in 50% v/v glycerol, in the dark, 1 month
-
4°C, about 10% loss of activity within 6 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli K12 strain NC629 harbouring multi-copy lambdaprbt-101 prophage which carries the rbt-operon
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