Information on EC 2.7.1.44 - galacturonokinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.44
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RECOMMENDED NAME
GeneOntology No.
galacturonokinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + D-galacturonate = ADP + 1-phospho-alpha-D-galacturonate
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
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UDP-alpha-D-galacturonate biosynthesis II (from D-galacturonate)
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SYSTEMATIC NAME
IUBMB Comments
ATP:D-galacturonate 1-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9026-63-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
buckwheat
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Manually annotated by BRENDA team
parsley
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Manually annotated by BRENDA team
pea
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-
Manually annotated by BRENDA team
radish
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-
Manually annotated by BRENDA team
mung bean
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-galacturonate
ADP + 1-phospho-alpha-D-galacturonate
show the reaction diagram
D-galacturonate + ATP
1-phospho-alpha-D-galacturonate + ADP
show the reaction diagram
wild-type protein: no activity with D-galactose, D-glucose, D-fructose, D-mannose, D-gluconic acid, D-xylose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, D-glucosamine, D-arabinose, L-arabinose, L-rhamnose,or L-Fucose, these monosaccharides (4 mM) have no effect on activity in the presence of galacturonic acid, neither CTP, GTP ITP,TTP, nor UTP are substrates
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-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-galacturonate
ADP + 1-phospho-alpha-D-galacturonate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP
addition (4 mM) results in 40% reduction of activity
additional information
no inhibition is observed by CTP, GTP ITP,TTP, or UTP (4 mM)
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.195
ATP
wild-type protein
0.0708 - 0.395
D-galacturonate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.76
Alpha-D-glucuronic acid
Arabidopsis thaliana
Q8VYG2
Y250F mutant protein
20112
6.3
ATP
Arabidopsis thaliana
Q8VYG2
wild-type protein
4
24 - 36
D-galacturonate
1752
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
no activity at 55C
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, crude recombinant enzyme is stable
-20C, purified enzyme preparations lose more than 90% of activity within one week
-80C, stable for several months when diluted with glycerol to 25% (v/v) and flash-frozen in liquid nitrogen
4C, 0.05 M mercaptoethanol, stable up to 10 days
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-tag, expressed in Escherichia coli BL21(de3)plysS
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A386S
possibly critical role in catalysis, relative activity: 90% (wild-type: 100%)
A41E
possibly critical for sugar binding, relative activity: 0% (wild-type: 100%)
Y250F
possibly important for substrate specificity (galacturonic acid /glucuronic acid), relative activity: 90% (wild-type: 100%), 50% activity with D-glucuronic acid (wild-type: 0%)