Information on EC 2.7.1.42 - riboflavin phosphotransferase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.7.1.42
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RECOMMENDED NAME
GeneOntology No.
riboflavin phosphotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-D-glucose 1-phosphate + riboflavin = D-glucose + FMN
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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Riboflavin metabolism
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SYSTEMATIC NAME
IUBMB Comments
alpha-D-glucose-1-phosphate:riboflavin 5'-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9026-26-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
neapolitanus, strain B-25
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-D-glucose 1-phosphate + riboflavin
alpha-D-glucose + FMN
show the reaction diagram
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-
-
-
?
beta-D-glucose 1-phosphate + riboflavin
beta-D-glucose + FMN
show the reaction diagram
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about 50% of activity compared to alpha-glucose 1-phosphate
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?
D-glucose 1-phosphate + 6-methyl-9-(D-1'-ribityl)-isoalloxazine
D-glucose + 6-methyl-9-(D-1'-ribityl)-isoalloxazine 5'-phosphate
show the reaction diagram
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93% of activity compared to riboflavin
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?
D-glucose 1-phosphate + araboflavin
D-glucose + araboflavin 5'-phosphate
show the reaction diagram
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93% of activity compared to riboflavin
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?
D-glucose 1-phosphate + isoriboflavin
D-glucose + isoriboflavin 5'-phosphate
show the reaction diagram
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73% of activity compared to riboflavin
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-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-D-glucose 1-phosphate + riboflavin
alpha-D-glucose + FMN
show the reaction diagram
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-
-
-
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additional information
?
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may be involved in biosynthesis of phosphate compounds that are essential for bacterial growth
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2MoO4
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0.5%: 100% inhibition
EDTA
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2 mM: 3% inhibition
fructose
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0.2 M: 24% inhibition
glucose
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0.2 M: 84% inhibition
KH2AsO4
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5 mM: 25% inhibition
KHSO4
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5 mM: 13% inhibition, 50 mM: 100% inhibition
maltose
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0.2 M: 20% inhibition
MgSO4
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0.1 mM: 7% inhibition
NaF
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25 mM, 100% inhibition
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.12
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pH 5.4, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3
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broad
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 8
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75% of maximal activity at pH 4, 20% of maximal activity at pH 8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, 1 week, stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE