Information on EC 2.7.1.27 - erythritol kinase (D-erythritol 4-phosphate-forming)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.27
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RECOMMENDED NAME
GeneOntology No.
erythritol kinase (D-erythritol 4-phosphate-forming)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + erythritol = ADP + D-erythritol 4-phosphate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
ATP:erythritol 4-phosphotransferase
The enzyme has been characterized from the bacterium Propionibacterium acidipropionici (previously known as Propionibacterium pentosaceum). cf. EC 2.7.1.215, erythritol kinase (L-erythritol 4-phosphate-forming).
CAS REGISTRY NUMBER
COMMENTARY hide
9030-64-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + erythritol
ADP + D-erythritol 1-phosphate
show the reaction diagram
ATP + erythritol
ADP + L-erythritol 1-phosphate
show the reaction diagram
ATP + meso-erythritol
ADP + meso-erythritol 1-phosphate
show the reaction diagram
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-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + erythritol
ADP + L-erythritol 1-phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
19% as effective as Mg2+
Mg2+
-
optimal Mg2+/ATP ratio: 2/1, 15% and 11% inhibition at ratios of 5/1 and 1/1, respectively
Mn2+
-
57% as effective as Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mg2+
-
optimal Mg2+/ATP ratio for activation: 2/1, inhibition at ratios of 5/1 and 1/1
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.8 - 9.8
ATP
0.876
erythritol
-
pH 8.6, 28°C
0.26
meso-erythritol
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pH 8.0, 25°C, recombinant erythritol kinase
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
230
ATP
Brucella abortus
-
pH 8.0, 25°C, recombinant erythritol kinase
230
meso-erythritol
Brucella abortus
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pH 8.0, 25°C, recombinant erythritol kinase
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
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approx. 55% of maximal activity at pH 6.0, approx. 80% of maximal activity at pH 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
3°C, 1 mg/ml bovine serum albumin, at least 1 month, no loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, Sephadex G-200
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recombinant enzyme, nickel affinity chromatography
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streptomycin, calcium phosphate gel, ammonium sulfate
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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