Information on EC 2.7.1.207 - protein-Npi-phosphohistidine-lactose phosphotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.207
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RECOMMENDED NAME
GeneOntology No.
protein-Npi-phosphohistidine-lactose phosphotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[protein]-Npi-phospho-L-histidine + lactose[side 1] = [protein]-L-histidine + lactose 6'-phosphate[side 2]
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Galactose metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
protein-Npi-phospho-L-histidine:lactose Npi-phosphotransferase
This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC 2.7.3.9 (phosphoenolpyruvate---protein phosphotransferase). Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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the enzyme has a crucial role in isopropyl beta-D-1-thiogalactoryranoside transport into the cell
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
[protein]-Npi-phospho-L-histidine + isopropyl beta-D-1-thiogalactoryranoside[side 1]
[protein]-L-histidine + isopropyl beta-D-1-thiogalactoryranose 6'-phosphate[side 2]
show the reaction diagram
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-
?
[protein]-Npi-phospho-L-histidine + lactose[side 1]
[protein]-L-histidine + lactose 6'-phosphate[side 2]
show the reaction diagram
[protein]-Npi-phospho-L-histidine + thio-4-digalactoside
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[protein]-Npi-phospho-L-histidine + isopropyl beta-D-1-thiogalactoryranoside[side 1]
[protein]-L-histidine + isopropyl beta-D-1-thiogalactoryranose 6'-phosphate[side 2]
show the reaction diagram
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?
[protein]-Npi-phospho-L-histidine + lactose[side 1]
[protein]-L-histidine + lactose 6'-phosphate[side 2]
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-(N-dansyl)aminoethyl alpha-D-thiogalactoside
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6-O-(4-toluenesulfonyl)galactose
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isopropyl thio-beta-D-galactoside
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methyl 6-O-(4-toluenesulfonyl) beta-D-galactoside
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methyl alpha-glucoside
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phosphotransferase protein IIAGlc
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protein IIAGlc binding inhibits conformational dynamics of the enzyme and decreases binding affinity for sugar
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additional information
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substrates of the phosphotransferase system inhibit uptake of lactose. This inhibition is reversed by intravesicular (but not extravesicular) phosphoenolpyruvate. lactose uptake is also inhibited by enzyme IIIGlc preparations that are shocked into the vesicles, and this inhibition is reversed by phosphoenolpyruvate. Enzyme I and HPr neither inhibit nor stimulate lactose uptake
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-lactate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0008
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unpurified His-tagged recombinant domain IIClac, at pH 7.6 and 37°C
0.0433
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54.1fold purified His-tagged recombinant domain IIClac, at pH 7.6 and 37°C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
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x * 20000, His-tagged domain IIALacS, SDS-PAGE
48000
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x * 48000, SDS-PAGE
61562
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x * 61562, calculated from amino acid sequence
62688
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x * 62688, calculated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gel filtration
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Ni-NTA column chromatography and MonoQ HR column chromatography
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Ni21 chelate affinity column chromatography
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S-Sepharose column chromatography and DEAE-Sepharose column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
domain IIC fused with a C-terminal affinity tag of six histidine residues and domain IIB fused with a N-terminal histidine tag are expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli
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expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli DH5alpha cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme IIAGlc mediates inhibition of lactose uptake and downregulation of the lac expression level
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isoforms PTS 6CB and PTS 8Care induced in the presence of lactose 107and 53fold, respectively
lactose permease activity in the overproducer strain of phosphocarrier protein of the phosphotransferase system, specific for glucose is reduced to an extremely low level in the presence of methyl alpha-glucoside
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the presence of isopropyl-beta-thiogalactoside plus cyclic AMP results in enhanced synthesis of the lactose permease
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C154G
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inactive
G46W/G262W
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inactive
I548E/G556D