Information on EC 2.7.1.205 - protein-Npi-phosphohistidine-cellobiose phosphotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.205
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RECOMMENDED NAME
GeneOntology No.
protein-Npi-phosphohistidine-cellobiose phosphotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[protein]-Npi-phospho-L-histidine + cellobiose[side 1] = [protein]-L-histidine + 6-phospho-beta-D-glucosyl-(1.>4)-D-glucose[side 2]
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
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SYSTEMATIC NAME
IUBMB Comments
protein-Npi-phospho-L-histidine:cellobiose Npi-phosphotransferase
This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC 2.7.3.9 (phosphoenolpyruvate---protein phosphotransferase). Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
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cellobiose transport in L. monocytogenes strain EGD-e is primarily performed by the PTSLac-4 permease and is supported by PTSLac. In the absence of these cellobiose-transporting PTSLac, at least PTSGlc-1 seems to function also as a low-affinity cellobiose transporter
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
[protein]-Npi-phospho-L-histidine + 4-methylumbelliferyl-4-D-cellobiopyranoside
?
show the reaction diagram
[protein]-Npi-phospho-L-histidine + D-cellobiose[side 1]
[protein]-L-histidine + D-cellobiose 6'-phosphate[side 2]
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[protein]-Npi-phospho-L-histidine + D-cellobiose[side 1]
[protein]-L-histidine + D-cellobiose 6'-phosphate[side 2]
show the reaction diagram
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
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calculated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
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expressed in Lactococcus lactis LL302 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the gene celB is tightly regulated by the general catabolite repression system and additionally requires the presence of cellobiose to be fully induced