Information on EC 2.7.1.191 - protein-Npi-phosphohistidine-D-mannose phosphotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.191
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RECOMMENDED NAME
GeneOntology No.
protein-Npi-phosphohistidine-D-mannose phosphotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[protein]-Npi-phospho-L-histidine + D-mannose[side 1] = [protein]-L-histidine + D-mannose 6-phosphate[side 2]
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
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Fructose and mannose metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
protein-Npi-phospho-L-histidine:D-mannose Npi-phosphotransferase
This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC 2.7.3.9 (phosphoenolpyruvate---protein phosphotransferase). Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
[protein]-Npi-phospho-L-histidine + 2-deoxy-D-glucose [side 1]
[protein]-L-histidine + 2-deoxy-D-glucose 6-phosphate[side 2]
show the reaction diagram
[protein]-Npi-phospho-L-histidine + 2-deoxy-D-glucose[side 1]
[protein]-L-histidine + 2-deoxy-D-glucose 6-phosphate[side 2]
show the reaction diagram
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-
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?
[protein]-Npi-phospho-L-histidine + 2-deoxyglucose[side 1]
[protein]-L-histidine + 2-deoxyglucose 6-phosphate[side 2]
show the reaction diagram
[protein]-Npi-phospho-L-histidine + D-fructose[side 1]
[protein]-L-histidine + D-fructose 6-phosphate[side 2]
show the reaction diagram
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-
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?
[protein]-Npi-phospho-L-histidine + D-glucosaminate[side 1]
[protein]-L-histidine + D-glucosaminate 6-phosphate[side 2]
show the reaction diagram
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?
[protein]-Npi-phospho-L-histidine + D-glucose[side 1]
[protein]-L-histidine + D-glucose 6-phosphate[side 2]
show the reaction diagram
[protein]-Npi-phospho-L-histidine + D-mannose[side 1]
[protein]-L-histidine + D-mannose 6-phosphate[side 2]
show the reaction diagram
[protein]-Npi-phospho-L-histidine + N-acetyl-D-glucosamine [side 1]
[protein]-L-histidine + N-acetyl-D-glucosamine 6-phosphate[side 2]
show the reaction diagram
additional information
?
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the enzyme is the major transporter for D-mannose and also transports efficiently D-glucose, 2-deoxyglucose, D-fructose, N-acetylglucosamine, and glucosamine
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[protein]-Npi-phospho-L-histidine + 2-deoxy-D-glucose[side 1]
[protein]-L-histidine + 2-deoxy-D-glucose 6-phosphate[side 2]
show the reaction diagram
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-
?
[protein]-Npi-phospho-L-histidine + D-fructose[side 1]
[protein]-L-histidine + D-fructose 6-phosphate[side 2]
show the reaction diagram
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-
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?
[protein]-Npi-phospho-L-histidine + D-glucosaminate[side 1]
[protein]-L-histidine + D-glucosaminate 6-phosphate[side 2]
show the reaction diagram
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?
[protein]-Npi-phospho-L-histidine + D-glucose[side 1]
[protein]-L-histidine + D-glucose 6-phosphate[side 2]
show the reaction diagram
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?
[protein]-Npi-phospho-L-histidine + D-mannose[side 1]
[protein]-L-histidine + D-mannose 6-phosphate[side 2]
show the reaction diagram
additional information
?
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the enzyme is the major transporter for D-mannose and also transports efficiently D-glucose, 2-deoxyglucose, D-fructose, N-acetylglucosamine, and glucosamine
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-fructose
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0.0015 mM almost totally inhibits uptake of D-mannose
D-mannose
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0.0105 mM almost totally inhibits uptake of D-mannose
additional information
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not inhibited by D-fructose
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Pel protein
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
2-deoxy-D-glucose[side 1]
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at pH 7.5 and 25°C
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0.02
D-fructose[side 1]
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at pH 7.5 and 25°C
0.015
D-glucose[side 1]
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at pH 7.5 and 25°C
0.045
D-mannose[side 1]
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at pH 7.5 and 25°C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21000
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x * 35000 + x * 27000 + x * 21000, SDS-PAGE
27640
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2 * 31020 + 1 * 27640, calculated from amino acid sequence
28000
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x * 3500 + x * 31000 + x * 28000, subunit IIIMan and subunit II-PMan and subunit II-MMan, SDS-PAGE
31020
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2 * 31020 + 1 * 27640, calculated from amino acid sequence
35200
x * 35200, subunit IIABLMan, calculated from amino acid sequence
35500
x * 35500, subunit IIABLMan, SDS-PAGE
72570
x * 72570, calculated from amino acid sequence
89700
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calculated from amino acid sequence
100000
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calculated from electronmicrographs of freeze fractured proteoliposomes containing particles of the HCMan/IIDMan subcomplex
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
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2 * 31020 + 1 * 27640, calculated from amino acid sequence
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli DH5alpha, BL21, and JM101 cells
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expressed in Escherichia coli JA300 cells
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expressed in Escherichia coli strain ZSCll3
expressed in Escherichia coli XL-1 Blue cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
after exposure to organic solvents like 10% (v/v) n-hexane, cyclohexane, p-xylene, or toluene, the expression levels of manX, manY, and manZ genes are strongly upregulated by 13.2, 10, and 7folds, respectively, after 2 min and then decrease after 10 min
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manX gene expression is negatively regulated by NagC and Mlc proteins
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small RNA SgrS-mediates down regulation of the enzyme
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H10C
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the phosphorylation site mutant of subunit IIABMan is inactive
H10C/H175C
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the phosphorylation site mutant of subunit IIABMan is inactive
H175C
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the phosphorylation site mutant of subunit IIABMan is inactive
H219Q
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the phosphorylation site mutant of subunit IIABMan show less than 7% of wild type activity
H86N
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the phosphorylation site mutant of subunit IIABMan is inactive
K48C
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the phosphorylation site mutant of subunit IIABMan shows more than 50% of wild type activity
S110C
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the phosphorylation site mutant of subunit IIABMan shows more than 50% of wild type activity
S72C
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the phosphorylation site mutant of subunit IIABMan show less than 7% of wild type activity
W12F
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the phosphorylation site mutant of subunit IIABMan show less than 7% of wild type activity
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