Information on EC 2.7.1.189 - autoinducer-2 kinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.189
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RECOMMENDED NAME
GeneOntology No.
autoinducer-2 kinase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + (S)-4,5-dihydroxypentane-2,3-dione = ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
autoinducer AI-2 degradation
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SYSTEMATIC NAME
IUBMB Comments
ATP:(S)-4,5-dihydroxypentane-2,3-dione 5-phosphotransferase
The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene lsrK in the lsrRK operon
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Manually annotated by BRENDA team
gene lsrK
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Manually annotated by BRENDA team
serovar Typhimurium, gene lsrK
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Manually annotated by BRENDA team
gene lsrK
-
-
Manually annotated by BRENDA team
gene lsrK
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (4S)-4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
show the reaction diagram
-
the kinase LsrK phosphorylates C5 of the open form of the substrate
-
-
?
ATP + 1,2-dideoxy-2-ethylhexo-3,4-diulose
ADP + 1,2-dideoxy-2-ethyl-6-O-phosphonohexo-3,4-diulose
show the reaction diagram
ATP + 1,2-dihydroxy-5-methylhexane-3,4-dione
ADP + 2-hydroxy-5-methyl-3,4-dioxohexyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxy-6,6-dimethylheptane-3,4-dione
ADP + 2-hydroxy-6,6-dimethyl-3,4-dioxoheptyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxy-6-methylheptane-3,4-dione
ADP + 2-hydroxy-6-methyl-3,4-dioxoheptyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxydecane-3,4-dione
ADP + 2-hydroxy-3,4-dioxodecyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxynonane-3,4-dione
ADP + 2-hydroxy-3,4-dioxononyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxyoctane-3,4-dione
ADP + 2-hydroxy-3,4-dioxooctyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxyundecane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoundecyl phosphate
show the reaction diagram
ATP + 1-cyclopropyl-3,4-dihydroxybutane-1,2-dione
ADP + 4-cyclopropyl-2-hydroxy-3,4-dioxobutyl phosphate
show the reaction diagram
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
show the reaction diagram
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + P: ADP + 2-hydroxy-3,4-dioxopentyl phosphate
show the reaction diagram
-
the kinase LsrK phosphorylates C5 of the open form of the substrate
-
-
?
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + (4S)-4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
show the reaction diagram
-
the kinase LsrK phosphorylates C5 of the open form of the substrate
-
-
?
ATP + 1,2-dideoxy-2-ethylhexo-3,4-diulose
ADP + 1,2-dideoxy-2-ethyl-6-O-phosphonohexo-3,4-diulose
show the reaction diagram
ATP + 1,2-dihydroxy-5-methylhexane-3,4-dione
ADP + 2-hydroxy-5-methyl-3,4-dioxohexyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxy-6,6-dimethylheptane-3,4-dione
ADP + 2-hydroxy-6,6-dimethyl-3,4-dioxoheptyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxy-6-methylheptane-3,4-dione
ADP + 2-hydroxy-6-methyl-3,4-dioxoheptyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxydecane-3,4-dione
ADP + 2-hydroxy-3,4-dioxodecyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxyheptane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoheptyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxyhexane-3,4-dione
ADP + 2-hydroxy-3,4-dioxohexyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxynonane-3,4-dione
ADP + 2-hydroxy-3,4-dioxononyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxyoctane-3,4-dione
ADP + 2-hydroxy-3,4-dioxooctyl phosphate
show the reaction diagram
ATP + 1,2-dihydroxyundecane-3,4-dione
ADP + 2-hydroxy-3,4-dioxoundecyl phosphate
show the reaction diagram
ATP + 1-cyclopropyl-3,4-dihydroxybutane-1,2-dione
ADP + 4-cyclopropyl-2-hydroxy-3,4-dioxobutyl phosphate
show the reaction diagram
ATP + 4,5-dihydroxypentane-2,3-dione
ADP + 2-hydroxy-3,4-dioxopentyl phosphate
show the reaction diagram
ATP + autoinducer-2
ADP + phosphorylated autoinducer-2
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-dihydroxydecane-3,4-dione
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1,2-dihydroxyheptane-3,4-dione
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1,2-dihydroxyhexane-3,4-dione
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weak inhibition
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1,2-dihydroxyoctane-3,4-dione
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4
1,2-dihydroxyheptane-3,4-dione
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pH and temperature not specified in the publication
1.1
1,2-dihydroxyhexane-3,4-dione
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pH and temperature not specified in the publication
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0.27
1,2-dihydroxyoctane-3,4-dione
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pH and temperature not specified in the publication
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1
4,5-dihydroxypentane-2,3-dione
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pH and temperature not specified in the publication
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0.15
ATP
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pH and temperature not specified in the publication
additional information
additional information
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the bi-substrate kinetic mechanism is rapid equilibrium ordered, Michaelis-Menten kinetics, overview
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.8
1,2-dihydroxyheptane-3,4-dione
1.5
1,2-dihydroxyoctane-3,4-dione
Salmonella enterica
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pH and temperature not specified in the publication
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7.6
4,5-dihydroxypentane-2,3-dione
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025
1,2-dihydroxydecane-3,4-dione
Salmonella enterica
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pH and temperature not specified in the publication
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0.0053
1,2-dihydroxyheptane-3,4-dione
Salmonella enterica
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pH and temperature not specified in the publication
0.05
1,2-dihydroxyhexane-3,4-dione
Salmonella enterica
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above, pH and temperature not specified in the publication
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0.005
1,2-dihydroxyoctane-3,4-dione
Salmonella enterica
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pH and temperature not specified in the publication
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His6-tagged enzyme from Escherichia coli strain BL21 to homogeneity by nickel affinity chromatography and gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene lsrK
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gene lsrK, encoded in the lsrKR operon where lsrK codes for an AI-2 kinase and lsrR encodes a repressor of the lsr operon, sequences important for the optimal transcription of lsrRK are located between -89 and -255 bp. LsrR regulates the expression of both lsrACDBFG and lsrRK operons. Transcription of the lsr locus is complex and may be driven by several promoters
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gene lsrK, overexpression in Escherichia coli strain LW12
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gene lsrK, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
cAMP-catabolite activator protein (CAP)-dependent activation of the lsr transcription. A cAMP-CAP-independent mechanism of lsr activation involves LsrR, the lsr operon repressor
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glycerol-3-phosphate inhibits lsr operon transcription activation by cAMP-catabolite activator protein, CAP. Dihydroxyacetone phosphate represses lsr transcription by a cAMP-CAP-independent mechanism involving LsrR, the lsr operon repressor, dihydroxyacetone phosphate causes repression by an LsrR-dependent and catabolite repression-independent route
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LsrR regulates the expression of both lsrACDBFG and lsrRK operons
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phosphorylated 4,5-dihydroxy-2,3-pentanedione (P-DPD) binds specifically to the repressor of the lsr operon, LsrR, consistent with P-DPD being the inducer of the lsr operon
transcription of lsrK is not regulated by luxS or AI-2
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information