Information on EC 2.7.1.186 - mevalonate-3-phosphate 5-kinase

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The expected taxonomic range for this enzyme is: Thermoplasma acidophilum

EC NUMBER
COMMENTARY hide
2.7.1.186
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RECOMMENDED NAME
GeneOntology No.
mevalonate-3-phosphate 5-kinase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + (R)-3-phosphomevalonate = ADP + (R)-3,5-bisphosphomevalonate
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mevalonate pathway III (archaea)
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Terpenoid backbone biosynthesis
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mevalonate metabolism
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SYSTEMATIC NAME
IUBMB Comments
ATP:(R)-3-phosphomevalonate 5-phosphotransferase
Mevalonate 3-kinase (EC 2.7.1.185) and mevalonate-3-phosphate-5-kinase act sequentially in an alternate mevalonate pathway in the archaeon Thermoplasma acidophilum.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (R)-3-phosphomevalonate
ADP + mevalonate 3,5-bisphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + (R)-3-phosphomevalonate
ADP + mevalonate 3,5-bisphosphate
show the reaction diagram
Q9HK44
mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum
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-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
(R)-3-phosphomevalonate
pH 8.0, 60°C, it is not possible to accurately determine the Km for mevalonate-3-phosphate-5-kinase, however, because the substrate is not commercially available and the detection limit of the assay is 0.03 mM substrate. The enzyme is still at Vmax when it is assayed with 0.06 mM mevalonate 3-phosphate, indicating that the Km is well below 0.06 mM
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9
(R)-3-phosphomevalonate
Thermoplasma acidophilum
Q9HK44
pH 8.0, 60°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
no significant pH dependence in the pH range of 6.5-9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
1 h, enzyme retains more than 95% of its activity
70
1 h, about 75% loss of activity
80
1 h, complete loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli