Information on EC 2.7.1.177 - L-threonine kinase

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The expected taxonomic range for this enzyme is: Salmonella enterica

EC NUMBER
COMMENTARY hide
2.7.1.177
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RECOMMENDED NAME
GeneOntology No.
L-threonine kinase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + L-threonine = ADP + O-phospho-L-threonine
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
aminopropanol phosphate biosynthesis I
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Porphyrin and chlorophyll metabolism
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aminopropanol phosphate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
ATP:L-threonine O3-phosphotransferase
The enzyme is involved in the de novo synthesis of adenosylcobalamin. It is specific for ATP and free L-threonine. In the bacterium Salmonella enterica the activity with CTP, GTP, or UTP is 6, 11, and 3% of the activity with ATP.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Streptomyces coelicolor
the protein CouR3, encoded by gene couR3, exhibits an ATPase activity similar to its close orthologue, the threonine kinase PduX, but it does not show a threonine kinase activity
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
involved in the biosynthesis of adenosylcobalamin
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + L-threonine
ADP + O-phospho-L-threonine
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-threonine
ADP + O-phospho-L-threonine
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0547 - 1.979
ATP
0.127 - 4.698
L-threonine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0173 - 0.408
ATP
0.016 - 0.0427
L-threonine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000104 - 0.00755
ATP
4
0.000061 - 0.00292
L-threonine
250
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32787
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x * 32787, calculated from sequence, x * 35000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nickel-nitrilotriacetate column
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D103N
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similar growth rate compared to wild-type
D135A
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no activity, decrease in helical structure
D135N
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similar growth rate compared to wild-type
E132A
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similar growth rate compared to wild-type
E132Q
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similar growth rate compared to wild-type
E24A
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no activity, decrease in helical structure
E24Q
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similar growth rate compared to wild-type
S100A
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no activity
S253A
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low growth rate, reduced affinity for L-threonine
S255A
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low growth rate, reduced affinity for L-threonine
S99A
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similar growth rate compared to wild-type
T101A
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similar growth rate compared to wild-type
T134A
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similar growth rate compared to wild-type