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(polyphosphate)n + D-xylulose
(polyphosphate)n-1 + D-xylulose 5-phosphate
ATP + 1-deoxy-D-xylulose
ADP + 1-deoxy-D-xylulose 5-phosphate
ATP + D(+)-arabitol
ADP + D(+)-arabitol 5-phosphate
ATP + D(-)-arabinose
ADP + D(-)arabinose 5-phosphate
ATP + D-arabitol
ADP + D-arabitol 5-phosphate
-
-
-
?
ATP + D-fructose
ADP + D-fructose 5-phosphate
ATP + D-glucose
ADP + D-glucose 6-phosphate
ATP + D-ribose
ADP + D-ribose 5-phosphate
ATP + D-ribulose
ADP + D-ribulose 5-phosphate
ATP + D-xylitol
ADP + ?
121% relative activity compared to D-glucose
-
-
?
ATP + D-xylitol
ADP + D-xylitol-1-phosphate
-
analyzed with 31P NMR spectrum
-
-
?
ATP + D-xylitol
ADP + D-xylitol-5-phosphate
ATP + D-xylose
ADP + D-xylose 5-phosphate
85% relative activity compared to D-glucose
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
ATP + L(-)-arabitol
ADP + L(-)-arabitol 5-phosphate
93% relative activity compared to D-glucose
-
-
?
ATP + L-arabinose
ADP + L-arabinose 5-phosphate
96% relative activity compared to D-glucose
-
-
?
ATP + L-erythrulose
ADP + ?
-
phosphorylation at 30% the rate of D-xylulose
-
-
?
ATP + L-ribulose
ADP + L-ribulose 5-phosphate
ATP + L-xylulose
ADP + L-xylulose 5-phosphate
ATP + ribitol
ADP + ?
41% relative activity compared to D-glucose
-
-
?
CTP + 1-deoxy-D-xylulose
CDP + 1-deoxy-D-xylulose 5-phosphate
D-xylulose + ATP
D-xylulose 5-phosphate + ADP
-
-
-
-
?
GTP + 1-deoxy-D-xylulose
GDP + 1-deoxy-D-xylulose 5-phosphate
-
38% of the activity with ATP
-
?
GTP + D-xylulose
GDP + D-xylulose 5-phosphate
ITP + 1-deoxy-D-xylulose
IDP + 1-deoxy-D-xylulose 5-phosphate
-
26% of the activity with ATP
-
?
ITP + D-xylulose
IDP + D-xylulose 5-phosphate
-
phosphorylation at 25% the rate of ATP
-
-
?
L-ribulose + ATP
L-ribulose 5-phosphate + ADP
-
-
-
-
?
UTP + 1-deoxy-D-xylulose
UDP + 1-deoxy-D-xylulose 5-phosphate
UTP + D-xylulose
UDP + D-xylulose 5-phosphate
additional information
?
-
(polyphosphate)n + D-xylulose
(polyphosphate)n-1 + D-xylulose 5-phosphate
activity with polyphosphate is 41 % of its specific activity on ATP
-
-
?
(polyphosphate)n + D-xylulose
(polyphosphate)n-1 + D-xylulose 5-phosphate
activity with polyphosphate is 41 % of its specific activity on ATP
-
-
?
ATP + 1-deoxy-D-xylulose
ADP + 1-deoxy-D-xylulose 5-phosphate
-
-
-
-
?
ATP + 1-deoxy-D-xylulose
ADP + 1-deoxy-D-xylulose 5-phosphate
-
-
-
?
ATP + 1-deoxy-D-xylulose
ADP + 1-deoxy-D-xylulose 5-phosphate
-
the reaction constitutes a potential salvage pathway for the generation of 1-deoxy-D-xylulose 5-phosphate from exogenous or endogenous 1-deoxy-D-xylulose as starting material for the biosynthesis of terpenoids, thiamine and pyridoxal
-
?
ATP + 1-deoxy-D-xylulose
ADP + 1-deoxy-D-xylulose 5-phosphate
-
-
-
?
ATP + 1-deoxy-D-xylulose
ADP + 1-deoxy-D-xylulose 5-phosphate
-
the reaction constitutes a potential salvage pathway for the generation of 1-deoxy-D-xylulose 5-phosphate from exogenous or endogenous 1-deoxy-D-xylulose as starting material for the biosynthesis of terpenoids, thiamine and pyridoxal
-
?
ATP + D(+)-arabitol
ADP + D(+)-arabitol 5-phosphate
76% relative activity compared to D-glucose
-
-
?
ATP + D(+)-arabitol
ADP + D(+)-arabitol 5-phosphate
76% relative activity compared to D-glucose
-
-
?
ATP + D(-)-arabinose
ADP + D(-)arabinose 5-phosphate
97% relative activity compared to D-glucose
-
-
?
ATP + D(-)-arabinose
ADP + D(-)arabinose 5-phosphate
97% relative activity compared to D-glucose
-
-
?
ATP + D-fructose
ADP + D-fructose 5-phosphate
-
-
-
?
ATP + D-fructose
ADP + D-fructose 5-phosphate
-
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
100% activity
-
-
?
ATP + D-glucose
ADP + D-glucose 6-phosphate
100% activity
-
-
?
ATP + D-ribose
ADP + D-ribose 5-phosphate
85% relative activity compared to D-glucose
-
-
?
ATP + D-ribose
ADP + D-ribose 5-phosphate
85% relative activity compared to D-glucose
-
-
?
ATP + D-ribulose
ADP + D-ribulose 5-phosphate
-
-
-
?
ATP + D-ribulose
ADP + D-ribulose 5-phosphate
-
60% of the activity with D-xylulose
-
-
?
ATP + D-ribulose
ADP + D-ribulose 5-phosphate
-
60% of the activity with D-xylulose
-
-
?
ATP + D-xylitol
ADP + D-xylitol-5-phosphate
-
-
-
?
ATP + D-xylitol
ADP + D-xylitol-5-phosphate
intracellular xylitol is partly converted to xylitol-5-phosphate by xylulokinase
-
-
?
ATP + D-xylitol
ADP + D-xylitol-5-phosphate
-
-
-
?
ATP + D-xylitol
ADP + D-xylitol-5-phosphate
intracellular xylitol is partly converted to xylitol-5-phosphate by xylulokinase
-
-
?
ATP + D-xylitol
ADP + D-xylitol-5-phosphate
-
analyzed with 31P NMR spectrum
-
-
?
ATP + D-xylulose
?
-
enzyme of ribitol catabolism, inducible by ribitol
-
-
?
ATP + D-xylulose
?
-
enzyme of ribitol catabolism, inducible by ribitol
-
-
?
ATP + D-xylulose
?
-
overexpression of xylulokinase in Saccharomyces cerevisiae is detrimental to cell growth on xylulose but not on glucose
-
-
?
ATP + D-xylulose
?
-
xylulokinase activity is absent during glucose metabolism but is detectable during simultaneous utilization of xylulose and glucose
-
-
?
ATP + D-xylulose
?
-
expression level of xylulokinase can limit xylulose metabolism, although other factors are also likely to have a role
-
-
?
ATP + D-xylulose
?
-
the enzyme is involved in one of the initial steps of xylose utilization
-
-
?
ATP + D-xylulose
?
-
expression level of xylulokinase can limit xylulose metabolism, although other factors are also likely to have a role
-
-
?
ATP + D-xylulose
?
-
-
-
-
?
ATP + D-xylulose
?
-
overexpression of xylulokinase in Saccharomyces cerevisiae is detrimental to cell growth on xylulose but not on glucose
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
L-Ribulose is not phosphorylated at significant rates
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
L-Ribulose is not phosphorylated at significant rates
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
specific for D-isomer
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
specific for D-isomer
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
specific for D-isomer
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
specific for D-isomer
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
?
ATP + L-ribulose
ADP + L-ribulose 5-phosphate
3.8% of the activity with D-xylulose
-
-
?
ATP + L-ribulose
ADP + L-ribulose 5-phosphate
3.8% of the activity with D-xylulose
-
-
?
ATP + L-xylulose
ADP + L-xylulose 5-phosphate
57.6% of the activity with D-xylulose
-
-
?
ATP + L-xylulose
ADP + L-xylulose 5-phosphate
57.6% of the activity with D-xylulose
-
-
?
ATP + xylitol
ADP + ?
-
-
-
?
ATP + xylitol
ADP + ?
-
poor substrate
-
-
?
CTP + 1-deoxy-D-xylulose
CDP + 1-deoxy-D-xylulose 5-phosphate
-
18% of the activity with ATP
-
?
CTP + 1-deoxy-D-xylulose
CDP + 1-deoxy-D-xylulose 5-phosphate
-
18% of the activity with ATP
-
?
GTP + D-xylulose
GDP + D-xylulose 5-phosphate
-
phosphorylation at 25% the rate of ATP
-
-
?
GTP + D-xylulose
GDP + D-xylulose 5-phosphate
-
no activity
-
-
?
UTP + 1-deoxy-D-xylulose
UDP + 1-deoxy-D-xylulose 5-phosphate
-
8% of the activity with ATP
-
?
UTP + 1-deoxy-D-xylulose
UDP + 1-deoxy-D-xylulose 5-phosphate
-
8% of the activity with ATP
-
?
UTP + D-xylulose
UDP + D-xylulose 5-phosphate
-
phosphorylation at 14% the rate of ATP
-
-
?
UTP + D-xylulose
UDP + D-xylulose 5-phosphate
-
15% of the activity with ATP
-
-
?
UTP + D-xylulose
UDP + D-xylulose 5-phosphate
-
no activity
-
-
?
additional information
?
-
D-ribulose is not a substrate
-
-
?
additional information
?
-
-
D-ribulose is not a substrate
-
-
?
additional information
?
-
no activity with D-xylose
-
-
?
additional information
?
-
-
no activity with D-xylose
-
-
?
additional information
?
-
no activity with D-xylose
-
-
?
additional information
?
-
-
no activity with D-xylose
-
-
?
additional information
?
-
-
enzyme is constitutively expressed
-
-
?
additional information
?
-
-
enzyme expression is not rate-limiting for xylose metabolism
-
-
?
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Abetalipoproteinemia
Expression of Kell blood group protein in nonerythroid tissues.
Abetalipoproteinemia
Expression profiles of mouse Kell, XK, and XPLAC mRNA.
Abetalipoproteinemia
Insights into extensive deletions around the XK locus associated with McLeod phenotype and characterization of two novel cases.
Abetalipoproteinemia
McLeod phenotype associated with a XK missense mutation without hematologic, neuromuscular, or cerebral involvement.
Abetalipoproteinemia
Neurodegeneration in the elderly - When the blood type matters: An overview of the McLeod syndrome with focus on hematological features.
Abetalipoproteinemia
Novel single base-pair deletion in exon 1 of XK gene leading to McLeod syndrome with chorea, muscle wasting, peripheral neuropathy, acanthocytosis and haemolysis.
Anencephaly
A review and case report of aprosencephaly and the XK aprosencephaly syndrome.
Anencephaly
Anomalies of the forebrain with radial limb defects: Garcia-Lurie-Steinfeld syndrome?
Anencephaly
XK aprosencephaly and anencephaly in sibs.
Anencephaly
XK aprosencephaly may be a new mutation or a dominant genetic defect.
Anencephaly
XK aprosencephaly.
Bradycardia
Effects of avertin versus xylazine-ketamine anesthesia on cardiac function in normal mice.
Cardiomyopathies
Analysis of the McLeod syndrome gene in three patients with neuroacanthocytosis.
Chorea
Novel single base-pair deletion in exon 1 of XK gene leading to McLeod syndrome with chorea, muscle wasting, peripheral neuropathy, acanthocytosis and haemolysis.
Chorea
Phenotypic variability of a distinct deletion in McLeod syndrome.
Chorea
The chorea of McLeod syndrome.
Chorea
[Progress in molecular chorea diagnosis. McLeod syndrome and chorea acanthocytosis]
Cleft Lip
Letter to the editor. Liu Q, Yang M L, Li Z J, Bai X F, Wang XK, Lu L, Wang Y X. A simple and precise classification for cleft lip and palate: a five digit numerical recording system. Cleft Palate Craniofac J. 2007;44:465-8.
Cleft Palate
Letter to the editor. Liu Q, Yang M L, Li Z J, Bai X F, Wang XK, Lu L, Wang Y X. A simple and precise classification for cleft lip and palate: a five digit numerical recording system. Cleft Palate Craniofac J. 2007;44:465-8.
Communicable Diseases
Assessment of Farmer Knowledge of Large Ruminant Health and Production in Developing Village-Level Biosecurity in Northern Lao PDR.
Coronary Artery Disease
Association between the XKR6 rs7819412 SNP and serum lipid levels and the risk of coronary artery disease and ischemic stroke.
Cystic Fibrosis
Cystic fibrosis in Afro-Brazilians: XK haplotypes analysis supports the European origin of p.F508del mutation.
Disorders of Sex Development
Ring chromosome 13 with loss of the region D13S317-D13S285: phenotypic overlap with XK syndrome.
Genetic Diseases, Inborn
Brain F-18 FDG and F-18 FP-CIT PET/CT Findings of c.856_860delCTCTA Mutation McLeod Syndrome.
Granulomatous Disease, Chronic
Gene deletion in a patient with chronic granulomatous disease and McLeod syndrome: fine mapping of the Xk gene locus.
Granulomatous Disease, Chronic
Insights into extensive deletions around the XK locus associated with McLeod phenotype and characterization of two novel cases.
Granulomatous Disease, Chronic
Kx: its relationship to chronic granulomatous disease and genetic linkage with Xg.
Granulomatous Disease, Chronic
Molecular Basis and Clinical Overview of McLeod Syndrome Compared With Other Neuroacanthocytosis Syndromes: A Review.
Granulomatous Disease, Chronic
Unusual muscle pathology in McLeod syndrome.
Hemoglobinuria
No evidence of hypermutability in red cells from patients with paroxysmal nocturnal hemoglobinuria using the XK gene.
Hemoglobinuria, Paroxysmal
No evidence of hypermutability in red cells from patients with paroxysmal nocturnal hemoglobinuria using the XK gene.
Huntington Disease
Molecular Basis and Clinical Overview of McLeod Syndrome Compared With Other Neuroacanthocytosis Syndromes: A Review.
Hydranencephaly
XK-aprosencephaly and related entities.
Ischemic Stroke
Association between the XKR6 rs7819412 SNP and serum lipid levels and the risk of coronary artery disease and ischemic stroke.
Metabolic Diseases
Structure and function of human xylulokinase, an enzyme with important roles in carbohydrate metabolism.
Movement Disorders
A novel XK gene mutation in a Taiwanese family with McLeod syndrome.
Muscular Atrophy
Phenotypic variability of a distinct deletion in McLeod syndrome.
Muscular Diseases
Expression of Kell blood group protein in nonerythroid tissues.
Muscular Diseases
Kell and XK immunohistochemistry in McLeod myopathy.
Muscular Diseases
McLeod phenotype associated with a XK missense mutation without hematologic, neuromuscular, or cerebral involvement.
Muscular Dystrophies
The Kell blood group system: Kell and XK membrane proteins.
Muscular Dystrophy, Duchenne
Insights into extensive deletions around the XK locus associated with McLeod phenotype and characterization of two novel cases.
Muscular Dystrophy, Duchenne
Molecular Basis and Clinical Overview of McLeod Syndrome Compared With Other Neuroacanthocytosis Syndromes: A Review.
Muscular Dystrophy, Duchenne
Unusual muscle pathology in McLeod syndrome.
Neoplasms
XK-aprosencephaly and related entities.
Neural Tube Defects
Severe brain and limb defects with possible autosomal recessive inheritance: a series of six cases and review of the literature.
Neuroacanthocytosis
Analysis of the McLeod syndrome gene in three patients with neuroacanthocytosis.
Neuroacanthocytosis
Computational identification of phospho-tyrosine sub-networks related to acanthocyte generation in neuroacanthocytosis.
Neuroacanthocytosis
Giant axon formation in mice lacking Kell, XK, or Kell and XK: animal models of McLeod neuroacanthocytosis syndrome.
Neuroacanthocytosis
McLeod syndrome and neuroacanthocytosis with a novel mutation in the XK gene.
Neuroacanthocytosis
Novel pathogenic XK mutations in McLeod syndrome and interaction between XK protein and chorein.
Neuroacanthocytosis
The ced-8 gene controls the timing of programmed cell deaths in C. elegans.
Neuroacanthocytosis
XK is a partner for VPS13A: A molecular link between Chorea-Acanthocytosis and McLeod syndrome.
Neuroacanthocytosis
[McLeod syndrome: Multisystem involvement associated with neuroacanthocytosis linked to X chromosome. report of two related cases]
Neurologic Manifestations
Identification and characterization of a novel XK splice site mutation in a patient with McLeod syndrome.
ornithine carbamoyltransferase deficiency
Insights into extensive deletions around the XK locus associated with McLeod phenotype and characterization of two novel cases.
Ornithine Carbamoyltransferase Deficiency Disease
Contiguous gene deletion syndrome in a female with ornithine transcarbamylase deficiency.
Ornithine Carbamoyltransferase Deficiency Disease
Insights into extensive deletions around the XK locus associated with McLeod phenotype and characterization of two novel cases.
Peripheral Nervous System Diseases
Novel single base-pair deletion in exon 1 of XK gene leading to McLeod syndrome with chorea, muscle wasting, peripheral neuropathy, acanthocytosis and haemolysis.
Polyneuropathies
A novel XK gene mutation in a Taiwanese family with McLeod syndrome.
Retinitis Pigmentosa
Insights into extensive deletions around the XK locus associated with McLeod phenotype and characterization of two novel cases.
Retinitis Pigmentosa
Molecular Basis and Clinical Overview of McLeod Syndrome Compared With Other Neuroacanthocytosis Syndromes: A Review.
Seizures
Phenotypic variability of a distinct deletion in McLeod syndrome.
Typhus, Epidemic Louse-Borne
Clinical features and laboratory diagnosis of XK or mite-borne typhus as observed in 102 cases in the Barrackpore area.
Typhus, Epidemic Louse-Borne
Epidemiological observations on XK or mite-borne typhus in Barrackpore, Bengal.
Typhus, Epidemic Louse-Borne
Epidemiology of XK Typhus in Jamshedpur.
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0.134
-
Pseudomonas putida S12 strain xylAB containing xylose isomerase and xylulokinase gene from Escherichia coli
1.6
-
reaction with 1-deoxy-D-xylulose
1.62
-
pH 7.5, temperature not specified in the publication
23.5
using D-xylulose as substrate, 50 mM Tris-HCl (pH 7.5), at 25°C
46
using D-fructose as substrate, 50 mM Tris-HCl (pH 7.5), at 25°C
51
-
reaction with D-xylulose
additional information
-
0.07 units/mg, at 37°C, in Tris-HCl buffer (pH 7.5) 100 mM, MgCl2 10 mM, NADH 0.15 mM, sorbitol dehydrogenase 2 units, and xylose 500 mM
additional information
-
3.1 U/mg protein (native substrate 5 mM xylulose), with 0.14 U/mg protein activity when using 75 mM xylitol as substrate (activity in range of background level), activities measured from cell lysates by monitoring NADH depletion, using an assay in which pentulokinase activity is coupled to pyruvate kinase and lactate dehydrogenase activities, reaction mixture (final concentrations indicated): 71 mM TrisHCl pH 7.5, 7.1 mM MgCl2, 1 mM EDTA, 50 mM KCl, 7.1 mM KF, 5 mM KCN, 1.4 mM ATP, 1 mM phosphoenolpyruvic acid, 0.3 mM NADH, 0.7 U/ml pyruvate kinase, 1 U/ml lactate dehydrogenase, and 5 mM D-xylulose or 75 mM xylitol
additional information
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3.8 U/mg protein (native substrate 5 mM xylulose), 0.76 U/mg protein (substrate 75 mM xylitol), activities measured from cell lysates by monitoring NADH depletion, using an assay in which pentulokinase activity is coupled to pyruvate kinase and lactate dehydrogenase activities, reaction mixture (final concentrations indicated): 71 mM TrisHCl pH 7.5, 7.1 mM MgCl2, 1 mM EDTA, 50 mM KCl, 7.1 mM KF, 5 mM KCN, 1.4 mM ATP, 1 mM phosphoenolpyruvic acid, 0.3 mM NADH, 0.7 U/ml pyruvate kinase, 1 U/ml lactate dehydrogenase, and 5 mM D-xylulose or 75 mM xylitol
additional information
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0.144 +/-0.008 U/mg protein, the transformant grown in YNB medium with 4% D-xylose showes 2-fold higher XK activity as compared to that of the wild-type strain, activity in cell extracts is determined spectrophotometrically at 37°C, assay mixture: Tris-HCl (pH 7.8) 50 mM, MgCl2 5 mM, NADH 0.2 mM, phosphoenolpyruvate 1 mM, D-xylulose 8.5 mM, lactate dehydrogenase 10 U, pyruvate kinase 0.05 U, ATP 2 mM
additional information
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0.366 +/-0.019 U/mg protein, up to 2.4-fold increase in specific activity of XK as compared to wild type strain CBS4732, XK activity in cell extracts is determined spectrophotometrically at 37°C, XK assay mixture containes Tris-HCl buffer (pH 7.8) 50 mM, MgCl2 5 mM, NADH 0.2 mM, phosphoenolpyruvate 1 mM, D-xylulose 8.5 mM, lactate dehydrogenase (EC 1.1.1.27) 10 U, pyruvate kinase (EC 2.7.1.40) 0.05 U, and ATP 2 mM, reaction is started with addition of cell extract, blank without pyruvate kinase and lactate dehydrogenase is used to minimize interference of XDH activity in Hansenula polymorpha.
additional information
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strain CBS4732, 0.156 +/-0.009 U/mg protein
additional information
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strain XRn/XDH/XK, 0.375 +/-0.021 U/mg protein, up to 2.4-fold increase in specific activity of XK as compared to wild type strain CBS4732, activity in cell extracts is determined spectrophotometrically at 37°C, assay mixture containes Tris-HCl buffer (pH 7.8) 50 mM, MgCl2 5 mM, NADH 0.2 mM, phosphoenolpyruvate 1 mM, D-xylulose 8.5 mM, lactate dehydrogenase (EC 1.1.1.27) 10 U, pyruvate kinase (EC 2.7.1.40) 0.05 U, and ATP 2 mM. The reaction is started with addition of cell extract, blank without pyruvate kinase and lactate dehydrogenase is used to minimize interference of XDH activity in Hansenula polymorpha.
additional information
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0.034 +/-0.002 U/mg, more than 2fold higher values than D-XR/XDH and D-XR/ARSdR, XK reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute.
additional information
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20.6 +/-0.3 Unit/mg at 25°C, approx. 3 mM D-xylulose 5-phosphate could be produced over 10 min at 25°C from 5 mM xylulose with 0.004 mg purified xylulokinase. With 2.5 mM xylulose, about 80% of conversion occur.
additional information
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strain D-XR/ARSdR/XK, 0.030 +/-0.002 U/mg, more than two-fold higher values than D-XR/XDH and D-XR/ARSdR, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute.
additional information
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strain D452-2 D-XR/ARSdR, 0.013 +/-0.001 U/mg, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute.
additional information
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strain D452-2 D-XR/XDH, 0.014 +/-0.002 U/mg, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute.
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