Information on EC 2.7.1.159 - inositol-1,3,4-trisphosphate 5/6-kinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.159
-
RECOMMENDED NAME
GeneOntology No.
inositol-1,3,4-trisphosphate 5/6-kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
show the reaction diagram
-
-
-
-
ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
show the reaction diagram
-
-
-
-
ATP + 1D-myo-inositol-1,3,4-trisphosphate = ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
show the reaction diagram
ATP + 1D-myo-inositol-1,3,4-trisphosphate = ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho-group transfer
-
-
Phosphorylation
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
1D-myo-inositol hexakisphosphate biosynthesis II (mammalian)
-
-
1D-myo-inositol hexakisphosphate biosynthesis V (from Ins(1,3,4)P3)
-
-
D-myo-inositol (1,4,5,6)-tetrakisphosphate biosynthesis
-
-
D-myo-inositol (3,4,5,6)-tetrakisphosphate biosynthesis
-
-
Inositol phosphate metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:1D-myo-inositol 1,3,4-trisphosphate 5-phosphotransferase
In humans, this enzyme, along with EC 2.7.1.127 (inositol-trisphosphate 3-kinase), EC 2.7.1.140 (inositol-tetrakisphosphate 5-kinase) and EC 2.7.1.158 (inositol pentakisphosphate 2-kinase) is involved in the production of inositol hexakisphosphate (InsP6). InsP6 is involved in many cellular processes, including mRNA export from the nucleus [2]. Yeasts do not have this enzyme, so produce InsP6 from Ins(1,4,5)P3 by the actions of EC 2.7.1.151 (inositol-polyphosphate multikinase) and EC 2.7.1.158 (inositol-pentakisphosphate 2-kinase) [2].
CAS REGISTRY NUMBER
COMMENTARY hide
288307-53-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
calf
-
-
Manually annotated by BRENDA team
strain HM-1
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
T-DNA insertion in a gene encoding a putative inositol 1,3,4-trisphosphate 5/6-kinase, i.e. ITPK2, dsm3, leads to a drought- and salt-hypersensitive mutant. Under drought stress conditions, the mutant has significantly less accumulation of osmolytes such as proline and soluble sugar and shows significantly reduced root volume, spikelet fertility, biomass, and grain yield with concomittant increase in malondialdehyde level Overexpression of DSM3 in rice results in drought- and salt-hypersensitive phenotypes and physiological changes similar to those in the mutant. Inositol trisphosphate level is decreased in the overexpressors under normal condition and drought stress
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1D-myo-inositol 1,3,4-trisphosphate + ATP
1D-myo-inositol 1,3,4,5-tetrakisphosphate + ADP
show the reaction diagram
A1KXK8
key enzyme in the inositol phosphate pathway and a negative regulator of osmotic stress signaling
-
-
?
1D-myo-inositol 1,3,4-trisphosphate + ATP
1D-myo-inositol 1,3,4,6-tetrakisphosphate + ADP
show the reaction diagram
A1KXK8
key enzyme in the inositol phosphate pathway and a negative regulator of osmotic stress signaling
-
-
?
1D-myo-inositol-1,3,4,5,6-pentakisphosphate + ADP
1D-myo-inositol-3,4,5,6-tetrakisphosphate + ATP
show the reaction diagram
-
enzyme catalyzes both forward and reverse reaction
putative regulator of Ca2+-activated chloride channels.
-
r
1D-myo-inositol-1,3,4,5-tetrakisphosphate + ATP
1D-myo-inositol-1,3,4,5,6-pentakisphosphate + ADP
show the reaction diagram
-
-
-
-
r
1D-myo-inositol-1,3,4,5-tetrakisphosphate + ATP
1D-myo-inositol-1,3,4,6-tetrakisphosphate + ATP
show the reaction diagram
enzyme displays isomerase activity
-
-
?
1D-myo-inositol-1,3,4,6-tetrakisphosphate + ATP
1D-myo-inositol-1,3,4,5,6-pentakisphosphate + ADP
show the reaction diagram
-
-
-
-
r
1D-myo-inositol-1,3,4-trisphosphate + ATP
1D-myo-inositol-1,3,4,5-tetrakisphosphate + ADP
show the reaction diagram
-
-
-
-
r
1D-myo-inositol-1,3,4-trisphosphate + ATP
1D-myo-inositol-1,3,4,6-tetrakisphosphate + ADP
show the reaction diagram
1D-myo-inositol-1,4,5,6-tetrakisphosphate + ATP
1D-myo-inositol-1,3,4,6-tetrakisphosphate + ATP
show the reaction diagram
AtITPK4 is more active against 1D-myo-inositol-1,4,5,6-tetrakisphosphate than against 1D-myo-inositol-3,4,5,6-tetrakisphosphate.
-
-
?
1D-myo-inositol-1,4,6-trisphosphate + 1D-myo-inositol-3,4,6-trisphosphate + 2 ATP
2 1D-myo-inositol-1,3,4,6-tetrakisphosphate + 2 ADP
show the reaction diagram
racemic mixture
-
-
?
1D-myo-inositol-3,4,5,6-tetrakisphosphate + ATP
1D-myo-inositol-1,3,4,6-tetrakisphosphate + ATP
show the reaction diagram
enzyme displays isomerase activity
-
-
?
ATP + 1D-myo-inositol 1,3,4-trisphosphate
ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
show the reaction diagram
-
-
-
?
ATP + 1D-myo-inositol 1,3,4-trisphosphate
ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
show the reaction diagram
ATP + 1D-myo-inositol 1,4,6-trisphosphate + 1D-myo-inositol 3,4,6-trisphosphate
ADP + inositol tetrakisphosphate
show the reaction diagram
racemic mix of phosphates
-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
-
-
?
ATP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
ADP + 1D-myo-inositol-1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
-
-
-
?
ATP + 1D-myo-inositol-1,3,4-trisphosphate
?
show the reaction diagram
-
-
-
?
ATP + 1D-myo-inositol-1,3,4-trisphosphate
ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
show the reaction diagram
ATP + 1D-myo-inositol-1,3,4-trisphosphate
ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
show the reaction diagram
ATP + ATF-2
ADP + phosphorylated ATF-2
show the reaction diagram
ATP + c-Jun
ADP + phosphorylated c-Jun
show the reaction diagram
ATP + IkappaBalpha
ADP + phosphorylated IkappaBalpha
show the reaction diagram
-
substrate of the free enzyme and the COP9 signalosome complex
-
-
?
ATP + p53
ADP + phosphorylated p53
show the reaction diagram
-
substrate of the free enzyme and the COP9 signalosome complex
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1D-myo-inositol 1,3,4-trisphosphate + ATP
1D-myo-inositol 1,3,4,5-tetrakisphosphate + ADP
show the reaction diagram
A1KXK8
key enzyme in the inositol phosphate pathway and a negative regulator of osmotic stress signaling
-
-
?
1D-myo-inositol 1,3,4-trisphosphate + ATP
1D-myo-inositol 1,3,4,6-tetrakisphosphate + ADP
show the reaction diagram
A1KXK8
key enzyme in the inositol phosphate pathway and a negative regulator of osmotic stress signaling
-
-
?
1D-myo-inositol-1,3,4,5,6-pentakisphosphate + ADP
1D-myo-inositol-3,4,5,6-tetrakisphosphate + ATP
show the reaction diagram
-
enzyme catalyzes both forward and reverse reaction
putative regulator of Ca2+-activated chloride channels.
-
r
1D-myo-inositol-1,3,4,5-tetrakisphosphate + ATP
1D-myo-inositol-1,3,4,5,6-pentakisphosphate + ADP
show the reaction diagram
-
-
-
-
r
1D-myo-inositol-1,3,4,5-tetrakisphosphate + ATP
1D-myo-inositol-1,3,4,6-tetrakisphosphate + ATP
show the reaction diagram
O80568, O81893, Q9SBA5, Q9SUG3
enzyme displays isomerase activity
-
-
?
1D-myo-inositol-1,3,4,6-tetrakisphosphate + ATP
1D-myo-inositol-1,3,4,5,6-pentakisphosphate + ADP
show the reaction diagram
-
-
-
-
r
1D-myo-inositol-1,3,4-trisphosphate + ATP
1D-myo-inositol-1,3,4,5-tetrakisphosphate + ADP
show the reaction diagram
-
-
-
-
r
1D-myo-inositol-1,3,4-trisphosphate + ATP
1D-myo-inositol-1,3,4,6-tetrakisphosphate + ADP
show the reaction diagram
1D-myo-inositol-1,4,6-trisphosphate + 1D-myo-inositol-3,4,6-trisphosphate + 2 ATP
2 1D-myo-inositol-1,3,4,6-tetrakisphosphate + 2 ADP
show the reaction diagram
O80568, O81893, Q9SBA5, Q9SUG3
racemic mixture
-
-
?
1D-myo-inositol-3,4,5,6-tetrakisphosphate + ATP
1D-myo-inositol-1,3,4,6-tetrakisphosphate + ATP
show the reaction diagram
O80568, O81893, Q9SBA5, Q9SUG3
enzyme displays isomerase activity
-
-
?
ATP + 1D-myo-inositol 1,3,4-trisphosphate
ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
show the reaction diagram
O80568, O81893, Q9SBA5, Q9SUG3
-
-
-
?
ATP + 1D-myo-inositol 1,3,4-trisphosphate
ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
show the reaction diagram
ATP + 1D-myo-inositol 1,4,6-trisphosphate + 1D-myo-inositol 3,4,6-trisphosphate
ADP + inositol tetrakisphosphate
show the reaction diagram
O80568, O81893, Q9SBA5, Q9SUG3
racemic mix of phosphates
-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
O80568, O81893, Q9SBA5, Q9SUG3
-
-
-
?
ATP + 1D-myo-inositol-1,3,4-trisphosphate
ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
show the reaction diagram
ATP + 1D-myo-inositol-1,3,4-trisphosphate
ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LiCl
10 mM in activity assay; 10 mM in activity assay; 10 mM in activity assay; 10 mM in activity assay
MgCl2
6 mM in activity assay; 6 mM in activity assay; 6 mM in activity assay; 6 mM in activity assay
Mn2+
-
activates the protein phopshorylation, but inhibits the inositol-1,3,4-trisphosphate 5/6-kinase reaction
NaCl
A1KXK8
Induced gene expression in rice by 200 mM. Level of relative germination in the two T3 transgenic tobacco lines overexpressing the OsITL1 gene is approximately 20-30% lower than that in the wild-type lines in the presence of 100 mM NaCl. In presence of 150 or 200 mM NaCl, seedling development is more significantly inhibited in transgenic tobacco plants than in wild-type plants. Transgenic plants that overexpress OsITL1 are more sensitive to NaCl stress during seed germination and early seedling development. The leaves of the transgenic seedlings show complete bleaching or extensive chlorosis after 7 days of exposure to 200 Mm NaCl
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
competitive
1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
competitive
1D-myo-inositol-3,4,5,6-tetrakisphosphate
-
inhibits the 5/6-kinase activities, but not the phosphorylation of ATF-2
curcumin
-
inhibits both the free enzyme and the COP9 signalosome complex-associated kinase
Mn2+
-
activates the protein phopshorylation, but inhibits the inositol-1,3,4-trisphosphate 5/6-kinase reaction
protein kinase A
-
slight inhibition of enzyme activity with ATF-2 as substrate
-
additional information
-
enzyme activity is unaffected by protein kinase C, protein kinase A, and Ca2+/calmodulin
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CSN1
-
the enzyme associates with the COP9 signalosome, an complex of 8 proteins, by binding to CSN1, activates
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.042
1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
0.00008
1D-myo-inositol-1,3,4-trisphosphate
-
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5 - 20
1D-myo-inositol-1,3,4-trisphosphate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003 - 0.004
1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
pH 7.2, 37C, versus 1D-myo-inositol-1,3,4-trisphosphate
0.002 - 0.003
1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
pH 7.2, 37C, versus 1D-myo-inositol-1,3,4-trisphosphate
0.00003
1D-myo-inositol-3,4,5,6-tetrakisphosphate
-
pH 7.5, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 48
-
calf brain
500
-
purified recombinant enzyme from Sf21 cells, with substrate 1D-myo-inositol-1,3,4-trisphosphate
1780
-
purified enzyme, determination of 5-kinase and 6-kinase activities
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
assay at; assay at; assay at; assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
sequence calculation
6.1
-
sequence calculation
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
young, AtITPK4 transcript is expressed throughout early flower development which culminates in the opening of the flower bud
Manually annotated by BRENDA team
additional information
ubiquitous enzyme expression in tissues
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38700
A1KXK8
predicted
60000
-
COP9 signalosome, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
the enzyme performs autophosphorylation
additional information
-
isoform ITPK1 can be acetylated by the acetyltransferases CREB-binding protein, CBP, and p300 both in vivo and in vitro and can be deacetylated by mammalian silent information regulator 2, SIRT1. Acetylation of ITPK1 decreases its enzyme activity and protein stability, and inhibits the synthesis of higher phosphorylated forms of inositol polyphosphates in the inositol signaling pathway. The acetylation sites are lysine residues 340, 383, and 410, which are all located on the surface of the protein
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant enzyme free or in complex with ATP analogue AMP-PCP and substrate inositol-1,3,4-trisphosphate in presence of Mg2+, 30 mg/ml protein in 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 10 mM DTT, mixed with a solution containing 20% PEG 3000, 0.1 M Tris-HCl, pH 7.5, and 0.1 M Ca(OAc)2, several months, crystals are seeded into sitting drops of 15 mg/ml protein, 25% PEG 3350, 5% PEG 400, 0.1 M Bis-Tris, pH 5.5, 10 mM DTT, and 10 mM L-cysteine, with or without ligands added as 10 mM AMP-PCP, 10 mM MgCl2, and 5 mM inositol-1,3,4-trisphosphate, direct X-ray diffraction analysis after this step or further seeding adding ADP and other ligands, overview, X-ray diffraction structure determination and analysis at 1.2-2.0 A resolution
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
addition of 1 mM ATP at pH 6.1 stabilizes the enzyme during purification
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
12900fold from brain by ion exchange chromatography, and heparin/inositol hexakisphosphate affinity chromatography
-
26171fold from liver by polyethylene glycol precipitation, ion exchange chromatography and heparin and inositol hexakisphosphate affinity chromatograpies
-
Cells are pelleted and resuspended in buffer, sonicated, centrifuged and the supernatant of the lysate is retained. Ni2+-nitrilotriacetate resin prewashed in NiSO4 is added to the lysate and incubated. The resin is washed twice with 10 ml of 50 mM NaH2PO4, 300 mM NaCl and 20 mM imidazole (pH 8) to elute non-specifically bound proteins, and washed with of NaH2PO4, 300 mM NaCl and 250 mM imidazole to elute AtIPK1 protein. Bacterial lysates and protein fractions from the purification are analysed by SDS/PAGE. Protein concentration is determined by Bradford assay.; Cells are pelleted and resuspended in buffer, sonicated, centrifuged and the supernatant of the lysate is retained. Ni2+-nitrilotriacetate resin prewashed in NiSO4 is added to the lysate and incubated. The resin is washed twice with 10 ml of 50 mM NaH2PO4, 300 mM NaCl and 20 mM imidazole (pH 8) to elute non-specifically bound proteins, and washed with of NaH2PO4, 300 mM NaCl and 250 mM imidazole to elute AtIPK1 protein. Bacterial lysates and protein fractions from the purification are analysed by SDS-PAGE. Protein concentration is determined by the Bradford assay.; Cells are pelleted and resuspended in buffer, sonicated, centrifuged and the supernatant of the lysate is retained. Ni2+-nitrilotriacetate resin prewashed in NiSO4 is added to the lysate and incubated. The resin is washed twice with 10 ml of 50 mM NaH2PO4, 300 mM NaCl and 20 mM imidazole (pH 8) to elute non-specifically bound proteins, and washed with of NaH2PO4, 300 mM NaCl and 250 mM imidazole to elute AtIPK1 protein. Bacterial lysates and protein fractions from the purification are analysed by SDS-PAGE. Protein concentration is determined by the Bradford method.; Cells are pelleted and resuspended in buffer, sonicated, centrifuged and the supernatant of the lysate is retained. Ni2+-nitrilotriacetate resin prewashed in NiSO4 is added to the lysate and incubated. The resin is washed twice with 10 ml of 50 mM NaH2PO4, 300 mM NaCl and 20 mM imidazole (pH 8) to elute non-specifically bound proteins, and washed with of NaH2PO4, 300 mM NaCl and 250 mM imidazole to elute AtIPK1 protein. Bacterial lysates and protein fractions from the purification are analysed by SDS/PAGE. Protein concentration is determined by the Bradford method.
partially as COP9 signalosome complex and further as a single protein
-
recombinant enzyme from insect Sf21 cells to homogeneity
-
recombinant enzyme from insect Sf21 cells, recombinant enzyme partially from 293 cells by ion exchange chromatography
-
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography and gel filtration
recombinant GST-tagged enzyme, the GST-tag is cleaved off by treatment with GST-3Cpro
recombinant His-tagged enzyme from Escherichia coli by adsorption and calmodulin affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloning and transformation of tobacco plants (Nicotiana tabacum L. cv. K326)
A1KXK8
DNA and amino acid sequence determination and analysis, expression as GST-tagged enzyme in bacteria
DNA and amino acid sequence determination and analysis, expression as tagged enzyme in Escherichia coli
-
DNA and N-terminal amino acid sequence determination and analysis
-
DNA and N-terminal amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli
expression in 293 cells, expression in Spodoptera frugiperda Sf21 cells using the baculovirus infection system
-
expression in Escherichia coli; in Escherichia coli Rosetta, transformed with pET28a and pET28c containing 3 kinase cDNAs. Vector-encoded hexa-histidine (His) tags are added to all proteins.; in Escherichia coli Rosetta, transformed with pET28a and pET28c containing 3 kinase cDNAs. Vector-encoded hexa-histidine (His) tags are added to all proteins.; in Escherichia coli Rosetta, transformed with pET28a and pET28c containing 3 kinase cDNAs. Vector-encoded hexa-histidine (His) tags are added to all proteins.; in Escherichia coli Rosetta, transformed with pET28a and pET28c containing 3 kinase cDNAs. Vector-encoded hexa-histidine (His) tags are added to all proteins.
expression in HeLa cells and in HEK-293 cells
-
expression in Sf9 cells and HEK-293 cells
-
expression of GST-tagged wild-type enzyme and of mutant enzymes in Escherichia coli
expression of His-tagged enzyme in Escherichia coli strain BL21
expression of the enzyme tagged with 6 repeats of Myc at the N-terminus in Spodoptera frugiperda Sf21 cells using the baculovirus infection system, expression of His6-tagged and of FLAG-tagged enzyme in Escherichia coli strain BL21(DE3), co-overexpression of the enzyme with CSN1 in HeLa cells
-
stable overexpression in HEK-293 cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
developmental expression
transcript level is induced about tenfold by drought and salt treatments and about 29fold induced by by abscisic acid, but only slightly induced by heat shock
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information