Information on EC 2.7.1.158 - inositol-pentakisphosphate 2-kinase

Word Map on EC 2.7.1.158
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.158
-
RECOMMENDED NAME
GeneOntology No.
inositol-pentakisphosphate 2-kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol hexakisphosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
phospho-group transfer
-
Phosphorylation
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
1D-myo-inositol hexakisphosphate biosynthesis I (from Ins(1,4,5)P3)
-
-
1D-myo-inositol hexakisphosphate biosynthesis II (mammalian)
-
-
1D-myo-inositol hexakisphosphate biosynthesis III (Spirodela polyrrhiza)
-
-
1D-myo-inositol hexakisphosphate biosynthesis IV (Dictyostelium)
-
-
1D-myo-inositol hexakisphosphate biosynthesis V (from Ins(1,3,4)P3)
-
-
Inositol phosphate metabolism
-
-
inositol pyrophosphates biosynthesis
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:1D-myo-inositol 1,3,4,5,6-pentakisphosphate 2-phosphotransferase
The enzyme can also use Ins(1,4,5,6)P4 [2] and Ins(1,4,5)P3 [3] as substrate. Inositol hexakisphosphate (phytate) accumulates in storage protein bodies during seed development and, when hydrolysed, releases stored nutrients to the developing seedling before the plant is capable of absorbing nutrients from its environment [5].
CAS REGISTRY NUMBER
COMMENTARY hide
134093-01-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
cultivar Hale
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ATP
1D-myo-inositol hexakisphosphate + ADP
show the reaction diagram
intermediate in both lipid-dependent and lipid-independent phytic acid biosynthetic pathways
phytic acid, in higher plants, phytic acid can mediate abscisic acid-induced guard cell closure by inactivating plasma membrane inward K+ conductance. During this process, phytic acid appears to act as an endomembrane calcium-releasing signal.
-
ir
1D-myo-inositol 1,4,6-trisphosphate + ATP
1D-myo-inositol 1,2,4,6-tetrakisphosphate + ADP
show the reaction diagram
relative substrate specifity 93.9%
-
-
?
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
ADP + 1D-myo-inositol hexakisphosphate
show the reaction diagram
ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
-
-
-
?
ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
?
show the reaction diagram
-
-
-
?
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
?
show the reaction diagram
-
-
-
?
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol hexakisphosphate
show the reaction diagram
-
-
-
-
?
ATP + 1D-myo-inositol 1,4,5-trisphosphate
ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
show the reaction diagram
-
-
-
-
?
ATP + 1D-myo-inositol 1,4,6-trisphosphate
ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
show the reaction diagram
-
-
-
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
?
show the reaction diagram
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
-
-
?
ATP + myo-inositol 1,3,4,5,6-pentakisphosphate
ADP + myo-inositol hexakisphosphate
show the reaction diagram
-
-
-
?
ATP + myo-inositol 1,4,6-trisphosphate
ADP + inositol 1,2,4,6-tetrakisphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ATP
1D-myo-inositol hexakisphosphate + ADP
show the reaction diagram
A4GWX8, A6YH13
intermediate in both lipid-dependent and lipid-independent phytic acid biosynthetic pathways
phytic acid, in higher plants, phytic acid can mediate abscisic acid-induced guard cell closure by inactivating plasma membrane inward K+ conductance. During this process, phytic acid appears to act as an endomembrane calcium-releasing signal.
-
ir
1D-myo-inositol 1,4,6-trisphosphate + ATP
1D-myo-inositol 1,2,4,6-tetrakisphosphate + ADP
show the reaction diagram
A4GWX8, A6YH13
relative substrate specifity 93.9%
-
-
?
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
ADP + 1D-myo-inositol hexakisphosphate
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
-
slightly inhibitory
1D-myo-inositol 1,4,6-trisphosphate
-
IC50: about 0.0007 mM. Potently reduces conversion of 1D-myo-inositol 1,4,5-trisphosphate to 1D-myo-inositol 1,4,5,6-tetrakisphosphate + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
Ca2+
-
20 mM, complete inhibition
N2-(m-trifluorobenzyl),N6-(p-nitrobenzyl)purine
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bovine serum albumin
-
0.0025%, increases activity of purified enzyme by 40%
-
Triton X-100
-
0.01%, increases activity
Tween 20
-
0.01%, increases activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0004 - 0.176
1D-myo-inositol 1,3,4,5,6-pentakisphosphate
0.06681
1D-myo-inositol 1,3,4,6-tetrakisphosphate
wild type enzyme, at pH 7.5 and 25°C
0.05599
1D-myo-inositol 1,4,5,6-tetrakisphosphate
wild type enzyme, at pH 7.5 and 25°C
0.00055
1D-myo-inositol 1,4,5-triphosphate
-
about, cosubstrate: ATP
0.04754 - 0.07917
1D-myo-inositol 3,4,5,6-tetrakisphosphate
0.0084 - 0.4
ATP
0.119
myo-inositol 1,3,4,5,6-pentakisphosphate
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.246 - 0.736
1D-myo-inositol 1,3,4,5,6-pentakisphosphate
0.4581
1D-myo-inositol 1,3,4,6-tetrakisphosphate
Arabidopsis thaliana
Q93YN9
wild type enzyme, at pH 7.5 and 25°C
0.11
1D-myo-inositol 1,4,5,6-tetrakisphosphate
Arabidopsis thaliana
Q93YN9
wild type enzyme, at pH 7.5 and 25°C
0.105 - 0.549
1D-myo-inositol 3,4,5,6-tetrakisphosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11.7 - 16.5
1D-myo-inositol 1,3,4,5,6-pentakisphosphate
3099
2.2 - 6.8
1D-myo-inositol 3,4,5,6-tetrakisphosphate
45062
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0007
1D-myo-inositol 1,4,6-trisphosphate
Schizosaccharomyces pombe
-
IC50: about 0.0007 mM. Potently reduces conversion of 1D-myo-inositol 1,4,5-trisphosphate to 1D-myo-inositol 1,4,5,6-tetrakisphosphate + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.031
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8
-
about 50% of maximal activity at pH 5.5 and pH 8.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 50
-
30°C: about 60% of maximal activity, 50°C: about 10% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
predicted
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
expression of Solanum tuberosum protein in Escherichia coli
Manually annotated by BRENDA team
-
; Purkinje layer of the cerebellum
Manually annotated by BRENDA team
immature ear
Manually annotated by BRENDA team
mature; maturing, ZmIPK1A
Manually annotated by BRENDA team
midstage; midstage, ZmIPK1A
Manually annotated by BRENDA team
-
XA232, with a gene trap construct embedded in the inositol 1,3,4,5,6-pentakisphosphate 2-kinase gene
Manually annotated by BRENDA team
-
string expression in male and female organs; strong expression of AtIPK1 in male and female organs of flower buds
Manually annotated by BRENDA team
-
of somites
Manually annotated by BRENDA team
-
the ventricular layer of the neural tube, at day 9.5 postcoitum, 2-kinase is expressed in the notochord; ventricular layer of neural tube, expressed at day 9.5 postcoitum
Manually annotated by BRENDA team
-
at day 9.5 postcoitum, 2-kinase is expressed in the notochord; expressed at day 9.5 postcoitum
Manually annotated by BRENDA team
; transcripts detected in ZmIPK1B; transcripts of ZmIPK1B were exclusively detected in roots
Manually annotated by BRENDA team
ZmIPK1A
Manually annotated by BRENDA team
-
; myotome of somites. At day 9.5 postcoitum, 2-kinase is expressed in the notochord
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
preferentially localized in euchromatin and nucleoli, and colocalized with mRNA
Manually annotated by BRENDA team
The majority of fusion protein is detected.
Manually annotated by BRENDA team
-
spatial microheterogeneity in the intracellular localization of IP5K in NRK cells, COS7 cell and H1299 overexpressing EGFP-IP5K fusion proteins, also confirmed for the endogenous enzyme
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48850
predicted
52000
-
x * 52000, SDS-PAGE
143000
-
Asp1-Pk1-green fluorescent protein, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
accumulation of soluble ZmIPK1 protein is very low under various growing temperatures and induction conditions, and the majority of the fusion protein is detected in inclusion bodies. Therefore, an alternative strategy using chaperone protein coexpression to enhance ZmIPK1 protein solubility is exploited.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, enzyme retains 50% of its activity after 6 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a glutathione affinity column is used to capture intact GST-ZmIPK1. The purified GST-ZmIPK1 fusion is cleaved with thrombin and further purified by a sequential affinity and ion-exchange chromatography. ZmIPK1 protein is eluted at 0.1 M NaCl on a high resolution Mono Q column with an estimated purity of 95%. Identity of the purified protein is confirmed via trypsin digestion followed by matrix-assisted laser deposition/ionization (MALDI) time-of-flight mass spectrometry analysis and comparison of the MALDI fingerprint to the amino acid sequences deduced from ZmIPK1.; expression in Escherichia coli; expression in Escherichia coli
by batch-elution from Ni-NTA affinity resin, Analysis by SDS-PAGE gel and Western Blot using an anti-His antibody. Purity of protein is >98% estimated by SDS-PAGE.
glutathione agarose bead chromatography
Ni-NTA bead chromatography
recombinant
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; production in Rat-1 cells
cloning and expression in Escherichia coli; expression in Escherichia coli; expression in Escherichia coli
Cloning from a potato tuber cDNA library and expression of StITPK1-His protein in Escherichia coli rosetta cells.
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-AI cells
expressed in Saccharomyces cerevisiae mutant strain Scipk1DELTA
-
expression in Escherichia coli; expressionof His tagged AtIPK1 protein in Escherichia coli
-
expression in Sf21 cells. Expression of the human enzyme in yeast ipk null strain restores synthesis of 1D-myo-inositol hexakisphosphate and rescues the gle1-2 ipk1-4 lethal phenotype
-
overexpression of EGFP-IP5K fusion proteins in NRK, COS7 and H1299 cells
-
spIpk1 functionally complements ipk1 mutants of Saccharomyces cerevisiae
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E82C/S142C
-
the mutant exhibits a dithiothreitol-sensitive 5fold increase in kcat for 3,4,5,6-inositol tetrakisphosphate compared to the wild type enzyme
H196A
the mutant shows reduced activity compared to the wild type enzyme
K200A
the mutant shows reduced activity compared to the wild type enzyme
N238A
the mutant shows reduced activity compared to the wild type enzyme
N239A
the mutant shows reduced activity compared to the wild type enzyme
R192A
the mutant shows reduced activity compared to the wild type enzyme
R415A
the mutant shows reduced activity compared to the wild type enzyme
R45A
the mutant shows reduced activity compared to the wild type enzyme
Y419A
the mutant shows reduced activity compared to the wild type enzyme
C162Y
-
inactive
D333A
-
kinase dead mutant of Asp1
H397A
-
phosphatase dead mutant of Asp1