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acetyl-phosphate + N-acetyl-alpha-D-galactosamine
acetate + N-acetyl-alpha-D-galactosamine 1-phosphate
-
8% of the specific activity with ATP
-
-
?
ATP + D-galactose
ADP + D-galactose 1-phosphate
ATP + N-acetyl-alpha-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
ATP + N-acetyl-alpha-D-glucosamine
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-mannosamine
ADP + N-acetyl-alpha-D-mannosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
ITP + N-acetyl-alpha-D-galactosamine
IDP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
9% of the specific activity with ATP
-
-
?
phosphoenolpyruvate + N-acetyl-alpha-D-galactosamine
pyruvate + N-acetyl-alpha-D-galactosamine 1-phosphate
-
11% of the specific activity with ATP
-
-
?
additional information
?
-
ATP + D-galactose
ADP + D-galactose 1-phosphate
-
phosphorylation of galactose at millimolar concentrations
-
-
?
ATP + D-galactose
ADP + D-galactose 1-phosphate
-
phosphorylation of galactose at millimolar concentrations
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
the enzyme is involved in a salvage pathway for reutilization of free GalNAc derived from the degradation of complex carbohydrates
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
-
?
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
-
?
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-D-galactosamine
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
-
?
additional information
?
-
-
ordered ternary complex mechanism in which ATP is the first substrate to bind
-
-
?
additional information
?
-
ordered ternary complex mechanism in which ATP is the first substrate to bind
-
-
?
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0.04 - 0.95
N-acetyl-alpha-D-galactosamine
0.051 - 0.39
N-acetyl-alpha-D-glucosamine
0.25 - 4.1
N-acetyl-alpha-D-mannosamine
0.04 - 0.14
N-acetyl-D-galactosamine
additional information
additional information
-
0.006
ATP
mutant F444A, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine
0.009
ATP
mutant F444R, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine
0.009
ATP
wild-type, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine
0.011
ATP
mutant F444H, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine
0.013
ATP
mutant F444H, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine
0.013
ATP
mutant F444S, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine
0.014
ATP
recombinant enzyme
0.015
ATP
mutant F444K, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine
0.015
ATP
mutant F444K, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine
0.015
ATP
mutant F444R, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine
0.017
ATP
mutant F444C, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine
0.017
ATP
mutant F444H, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine
0.018
ATP
mutant F444C, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine
0.018
ATP
wild-type, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine
0.019
ATP
mutant F444S, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine
0.024
ATP
mutant F444R, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine
0.024
ATP
mutant F444W, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine
0.025
ATP
mutant F444A, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-glucosamine
0.027
ATP
mutant F444C, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-galactosamine
0.028
ATP
mutant F444K, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine
0.032
ATP
wild-type, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine
0.039
ATP
mutant F444A, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine
0.13
ATP
mutant F444S, 37°C, pH 7.5, cosubstrate N-acetyl-alpha-D-mannosamine
0.04
N-acetyl-alpha-D-galactosamine
pH 8.0, 37°C
0.17
N-acetyl-alpha-D-galactosamine
mutant F444R, 37°C, pH 7.5
0.24
N-acetyl-alpha-D-galactosamine
mutant F444A, 37°C, pH 7.5
0.27
N-acetyl-alpha-D-galactosamine
mutant F444H, 37°C, pH 7.5
0.33
N-acetyl-alpha-D-galactosamine
wild-type, 37°C, pH 7.5
0.34
N-acetyl-alpha-D-galactosamine
mutant F444K, 37°C, pH 7.5
0.56
N-acetyl-alpha-D-galactosamine
mutant F444S, 37°C, pH 7.5
0.69
N-acetyl-alpha-D-galactosamine
mutant F444W, 37°C, pH 7.5
0.95
N-acetyl-alpha-D-galactosamine
mutant F444C, 37°C, pH 7.5
0.051
N-acetyl-alpha-D-glucosamine
wild-type, 37°C, pH 7.5
0.12
N-acetyl-alpha-D-glucosamine
mutant F444K, 37°C, pH 7.5
0.12
N-acetyl-alpha-D-glucosamine
mutant F444S, 37°C, pH 7.5
0.22
N-acetyl-alpha-D-glucosamine
mutant F444H, 37°C, pH 7.5
0.28
N-acetyl-alpha-D-glucosamine
mutant F444R, 37°C, pH 7.5
0.37
N-acetyl-alpha-D-glucosamine
mutant F444A, 37°C, pH 7.5
0.39
N-acetyl-alpha-D-glucosamine
mutant F444C, 37°C, pH 7.5
0.25
N-acetyl-alpha-D-mannosamine
mutant F444H, 37°C, pH 7.5
0.47
N-acetyl-alpha-D-mannosamine
mutant F444C, 37°C, pH 7.5
0.65
N-acetyl-alpha-D-mannosamine
mutant F444R, 37°C, pH 7.5
0.79
N-acetyl-alpha-D-mannosamine
wild-type, 37°C, pH 7.5
0.93
N-acetyl-alpha-D-mannosamine
mutant F444A, 37°C, pH 7.5
0.95
N-acetyl-alpha-D-mannosamine
mutant F444K, 37°C, pH 7.5
4.1
N-acetyl-alpha-D-mannosamine
mutant F444S, 37°C, pH 7.5
0.04
N-acetyl-D-galactosamine
recombinant enzyme
0.12
N-acetyl-D-galactosamine
-
pH 7.0, 25°C
0.14
N-acetyl-D-galactosamine
-
-
additional information
additional information
-
-
-
additional information
additional information
-
enzyme kinetics and thermodynamics, detailed overview
-
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0.62 - 3.2
N-acetyl-alpha-D-galactosamine
0.063 - 0.18
N-acetyl-alpha-D-glucosamine
0.053 - 0.4
N-acetyl-alpha-D-mannosamine
1 - 2.5
N-acetyl-D-galactosamine
1
ATP
recombinant enzyme
0.62
N-acetyl-alpha-D-galactosamine
mutant F444W, 37°C, pH 7.5
0.72
N-acetyl-alpha-D-galactosamine
mutant F444R, 37°C, pH 7.5
1
N-acetyl-alpha-D-galactosamine
pH 8.0, 37°C
1
N-acetyl-alpha-D-galactosamine
mutant F444K, 37°C, pH 7.5
1.2
N-acetyl-alpha-D-galactosamine
wild-type, 37°C, pH 7.5
1.6
N-acetyl-alpha-D-galactosamine
mutant F444H, 37°C, pH 7.5
1.9
N-acetyl-alpha-D-galactosamine
mutant F444A, 37°C, pH 7.5
2.6
N-acetyl-alpha-D-galactosamine
mutant F444C, 37°C, pH 7.5
3.2
N-acetyl-alpha-D-galactosamine
mutant F444S, 37°C, pH 7.5
0.063
N-acetyl-alpha-D-glucosamine
mutant F444S, 37°C, pH 7.5
0.071
N-acetyl-alpha-D-glucosamine
mutant F444R, 37°C, pH 7.5
0.072
N-acetyl-alpha-D-glucosamine
mutant F444H, 37°C, pH 7.5
0.087
N-acetyl-alpha-D-glucosamine
mutant F444C, 37°C, pH 7.5
0.11
N-acetyl-alpha-D-glucosamine
mutant F444A, 37°C, pH 7.5
0.16
N-acetyl-alpha-D-glucosamine
wild-type, 37°C, pH 7.5
0.18
N-acetyl-alpha-D-glucosamine
mutant F444K, 37°C, pH 7.5
0.053
N-acetyl-alpha-D-mannosamine
mutant F444H, 37°C, pH 7.5
0.066
N-acetyl-alpha-D-mannosamine
mutant F444C, 37°C, pH 7.5
0.19
N-acetyl-alpha-D-mannosamine
mutant F444R, 37°C, pH 7.5
0.19
N-acetyl-alpha-D-mannosamine
wild-type, 37°C, pH 7.5
0.23
N-acetyl-alpha-D-mannosamine
mutant F444A, 37°C, pH 7.5
0.25
N-acetyl-alpha-D-mannosamine
mutant F444K, 37°C, pH 7.5
0.4
N-acetyl-alpha-D-mannosamine
mutant F444S, 37°C, pH 7.5
1
N-acetyl-D-galactosamine
recombinant enzyme
2.5
N-acetyl-D-galactosamine
-
pH 7.0, 25°C
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GALK2_HUMAN
458
0
50378
Swiss-Prot
Mitochondrion (Reliability: 5)
GALK2_MOUSE
458
0
50503
Swiss-Prot
other Location (Reliability: 4)
GALK2_PONAB
458
0
50351
Swiss-Prot
Mitochondrion (Reliability: 5)
GALK2_RAT
458
0
50198
Swiss-Prot
other Location (Reliability: 4)
A0A5B7AAE4_DAVIN
405
0
44409
TrEMBL
other Location (Reliability: 1)
A0A108U7I3_9GAMM
285
0
29228
TrEMBL
-
B5X1Z3_SALSA
459
0
50428
TrEMBL
other Location (Reliability: 2)
A0A8B6FKZ4_MYTGA
455
0
49781
TrEMBL
other Location (Reliability: 2)
A0A7U9E132_STRLI
330
0
34500
TrEMBL
-
A0A5B7AA92_DAVIN
413
0
45227
TrEMBL
other Location (Reliability: 1)
A0A5B7AA30_DAVIN
499
0
54811
TrEMBL
other Location (Reliability: 1)
A0A2T6J5M2_TOXGO
923
0
98711
TrEMBL
Mitochondrion (Reliability: 1)
A0A086KUS3_TOXGO
923
0
98711
TrEMBL
Mitochondrion (Reliability: 1)
A0A086PRU0_TOXGO
501
0
54931
TrEMBL
other Location (Reliability: 5)
A0A812BNN9_SEPPH
461
0
50745
TrEMBL
other Location (Reliability: 1)
B7PKS9_IXOSC
473
0
51349
TrEMBL
other Location (Reliability: 2)
A0A061I297_CRIGR
488
0
53874
TrEMBL
other Location (Reliability: 3)
A0A8B6FNJ1_MYTGA
446
0
48759
TrEMBL
other Location (Reliability: 2)
A0A8M1N3C3_DANRE
457
0
50058
TrEMBL
other Location (Reliability: 4)
B9RZT4_RICCO
499
0
54449
TrEMBL
other Location (Reliability: 2)
A0A086PRV7_TOXGO
498
0
52983
TrEMBL
Mitochondrion (Reliability: 1)
D3PI35_LEPSM
441
0
49310
TrEMBL
other Location (Reliability: 3)
S7V341_TOXGG
Toxoplasma gondii (strain ATCC 50853 / GT1)
923
0
98711
TrEMBL
Mitochondrion (Reliability: 1)
A0A086QYA7_TOXGO
774
0
83449
TrEMBL
Mitochondrion (Reliability: 1)
A0A2G8Y837_TOXGO
752
0
80990
TrEMBL
Mitochondrion (Reliability: 1)
A0A7G2IQ11_CITFR
243
0
26637
TrEMBL
-
A0A5B7AF25_DAVIN
454
0
50308
TrEMBL
other Location (Reliability: 1)
A0A5B7A9W9_DAVIN
499
0
54755
TrEMBL
other Location (Reliability: 1)
A0A139Y4B5_TOXGO
752
0
81122
TrEMBL
Mitochondrion (Reliability: 1)
Q7ZXI6_XENLA
460
0
50079
TrEMBL
other Location (Reliability: 3)
A0A061I4C8_CRIGR
457
0
50301
TrEMBL
other Location (Reliability: 2)
A0A2G8Y823_TOXGO
170
0
17884
TrEMBL
other Location (Reliability: 2)
A0A5B7ACT6_DAVIN
499
0
54689
TrEMBL
other Location (Reliability: 1)
A0A3R8C2C1_TOXGO
923
0
98711
TrEMBL
Mitochondrion (Reliability: 1)
B6KJ89_TOXGV
Toxoplasma gondii (strain ATCC 50861 / VEG)
923
0
99031
TrEMBL
Mitochondrion (Reliability: 1)
A0A086M3Q4_TOXGO
752
0
80961
TrEMBL
Mitochondrion (Reliability: 1)
A0A125YV41_TOXGM
Toxoplasma gondii (strain ATCC 50611 / Me49)
923
0
99031
TrEMBL
Mitochondrion (Reliability: 1)
A0A086L283_TOXGO
803
0
86518
TrEMBL
other Location (Reliability: 5)
A0A139Y4H2_TOXGO
170
0
17910
TrEMBL
other Location (Reliability: 2)
Q6DJ33_XENTR
460
0
50230
TrEMBL
Mitochondrion (Reliability: 5)
A0A086M3Q6_TOXGO
22
0
2174
TrEMBL
other Location (Reliability: 4)
A0A086LFZ6_TOXGO
923
0
98711
TrEMBL
Mitochondrion (Reliability: 1)
A0A5B7ACH7_DAVIN
430
0
47064
TrEMBL
other Location (Reliability: 1)
A0A7G2IMR9_CITFR
78
0
9010
TrEMBL
-
A0A6J8DAJ1_MYTCO
455
0
49956
TrEMBL
other Location (Reliability: 2)
A0A086M3R6_TOXGO
149
0
15481
TrEMBL
other Location (Reliability: 2)
A0A086QYA4_TOXGO
149
0
15524
TrEMBL
other Location (Reliability: 2)
A0A8B6C929_MYTGA
444
0
48483
TrEMBL
other Location (Reliability: 2)
A0A5B7ADP0_DAVIN
368
0
40396
TrEMBL
other Location (Reliability: 1)
E0VT04_PEDHC
471
0
52820
TrEMBL
other Location (Reliability: 2)
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F444A
mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme
F444C
mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme but increases turnover rate with N-acetylgalactosamine
F444H
mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme
F444K
mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme
F444R
mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme
F444S
mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme but increases turnover rate with N-acetylgalactosamine
F444W
mutation in residue equivalent to residue Tyr379 of galactokinase, whose alteration dramatically enhances the substrate range of this enzyme. Mutation does not result in large changes to the specificity of the enzyme
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Pastuszak, I.; Drake, R.; Elbein, A.D.
Kidney N-acetylgalactosamine (GalNAc)-1-phosphate kinase, a new pathway of GalNAc activation
J. Biol. Chem.
271
20776-20782
1996
Oryctolagus cuniculus, Homo sapiens, Sus scrofa
brenda
Pastuszak, I.; O'Donnell, J.; Elbein, A.D.
Identification of the GalNAc kinase amino acid sequence
J. Biol. Chem.
271
23653-23656
1996
Homo sapiens, Sus scrofa
brenda
Thoden, J.B.; Holden, H.M.
The molecular architecture of human N-acetylgalactosamine kinase
J. Biol. Chem.
280
32784-32791
2005
Homo sapiens
brenda
Bourgeaux, V.; Piller, F.; Piller, V.
Two-step enzymatic synthesis of UDP-N-acetylgalactosamine
Bioorg. Med. Chem. Lett.
15
5459-5462
2005
Homo sapiens
brenda
Stockbridge, R.B.; Wolfenden, R.
The intrinsic reactivity of ATP and the catalytic proficiencies of kinases acting on glucose, N-acetylgalactosamine, and homoserine: a thermodynamic analysis
J. Biol. Chem.
284
22747-22757
2009
Homo sapiens
brenda
Agnew, A.; Timson, D.
Mechanistic studies on human N-acetylgalactosamine kinase
J. Enzyme Inhib. Med. Chem.
25
370-376
2009
Homo sapiens, Homo sapiens (Q01415)
brenda
Kristiansson, H.; Timson, D.J.
N-acetylgalactosamine kinase: a naturally promiscuous small molecule kinase
Appl. Biochem. Biotechnol.
166
57-63
2012
Homo sapiens (Q01415), Homo sapiens
brenda