Information on EC 2.7.1.143 - diphosphate-purine nucleoside kinase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.7.1.143
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RECOMMENDED NAME
GeneOntology No.
diphosphate-purine nucleoside kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
diphosphate + a purine nucleoside = phosphate + a purine mononucleotide
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho-group transfer
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CAS REGISTRY NUMBER
COMMENTARY hide
70356-41-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain B-PG9
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-
Manually annotated by BRENDA team
strain B-PG9
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-
Manually annotated by BRENDA team
Anaeroplasma intermedium
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-
Manually annotated by BRENDA team
4 of 8 strains have this enzyme activity
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-
Manually annotated by BRENDA team
no activity in Acholeplasma florum
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-
Manually annotated by BRENDA team
no activity in Mycoplasma arginini
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-
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Manually annotated by BRENDA team
no activity in Mycoplasma bovigenitalium
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-
-
Manually annotated by BRENDA team
no activity in Mycoplasma gallisepticum
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-
Manually annotated by BRENDA team
no activity in Mycoplasma genitalium
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-
Manually annotated by BRENDA team
no activity in Mycoplasma hyopneumoniae
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Manually annotated by BRENDA team
no activity in Mycoplasma pneumoniae
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-
-
Manually annotated by BRENDA team
no activity in Spiroplasma floricola
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-
Manually annotated by BRENDA team
no activity in Ureaplasma urealyticum
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
strain 3001
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-
Manually annotated by BRENDA team
strain 3001
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
diphosphate + adenosine
monophosphate + AMP
show the reaction diagram
diphosphate + adenosine
phosphate + AMP
show the reaction diagram
diphosphate + guanosine
monophosphate + GMP
show the reaction diagram
diphosphate + guanosine
phosphate + GMP
show the reaction diagram
diphosphate + inosine
monophosphate + IMP
show the reaction diagram
diphosphate + inosine
phosphate + IMP
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
diphosphate + adenosine
monophosphate + AMP
show the reaction diagram
diphosphate + adenosine
phosphate + AMP
show the reaction diagram
diphosphate + guanosine
phosphate + GMP
show the reaction diagram
diphosphate + inosine
phosphate + IMP
show the reaction diagram
-
-
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Formycin A
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IC50 is 0.00027 mM, inhibits growth of Trichomonas vaginalis, addition of adenine prevents growth inhibition, but not the addition of guanine and hypoxanthine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.84
2-deoxy-adenosine
Burkholderia thailandensis
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75751
6.05
adenosine
Burkholderia thailandensis
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-
122
16
ATP
Burkholderia thailandensis
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4
0.24
guanosine
Burkholderia thailandensis
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296
80
Inosine
Burkholderia thailandensis
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inosine is the most effective phosphoryl acceptor, with the highest kcat /Km value being achieved when ATP served as the phosphoryl donor
167
0.04
mizoribine
Burkholderia thailandensis
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5001
0.08
ribavirin
Burkholderia thailandensis
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1304
0.28
uridine
Burkholderia thailandensis
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261
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00027
Formycin A
Trichomonas vaginalis
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IC50 is 0.00027 mM, inhibits growth of Trichomonas vaginalis, addition of adenine prevents growth inhibition, but not the addition of guanine and hypoxanthine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially by ATP affinity chromatography
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