Information on EC 2.7.1.140 - inositol-tetrakisphosphate 5-kinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.140
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RECOMMENDED NAME
GeneOntology No.
inositol-tetrakisphosphate 5-kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
1D-myo-inositol hexakisphosphate biosynthesis II (mammalian)
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1D-myo-inositol hexakisphosphate biosynthesis IV (Dictyostelium)
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1D-myo-inositol hexakisphosphate biosynthesis V (from Ins(1,3,4)P3)
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D-myo-inositol (1,4,5,6)-tetrakisphosphate biosynthesis
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D-myo-inositol (3,4,5,6)-tetrakisphosphate biosynthesis
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Inositol phosphate metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
ATP:1D-myo-inositol-1,3,4,6-tetrakisphosphate 5-phosphotransferase
The enzyme from plants and yeast can also use Ins(1,2,3,4,6)P5 as a substrate [2].
CAS REGISTRY NUMBER
COMMENTARY hide
123940-40-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
Sprague-Dawley strain
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + myo-inositol 1,3,4,5-tetrakisphosphate
ADP + myo-inositol-1,3,4,5,6-pentakisphosphate
show the reaction diagram
-
approx. 2% of activity with 1,3,4,6-tetrakisphosphate
-
?
ATP + myo-inositol 1,3,4,6-tetrakisphosphate
ADP + myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
ATP + myo-inositol 1,4,5-trisphosphate
ADP + myo-inositol-1,3,4,5-tetrakisphosphate
show the reaction diagram
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approx. 50% of activity with 1D-myo-inositol 1,3,4,6-tetrakisphosphate, no activity with 1D-myo-inositol 1,3,4-trisphosphate
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + myo-inositol 1,3,4,6-tetrakisphosphate
ADP + myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00013
myo-inositol-1,3,4,5-tetrakisphosphate
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pH 7.2, 37°C
0.0003
myo-inositol-1,3,4,6-tetrakisphosphate
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pH 7.2, 37°C
0.00011
myo-inositol-1,4,5-trisphosphate
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pH 7.2, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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permeabilized
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme, cobalt-affinity column
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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gene silencing of enzyme results in decreased inositol pentakisphosphate and inositol hexakisphosphate levels
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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overexpression of enzyme does not increase the level of inositolpentakisphosphate, gene silencing of enzyme results in decreased inositol pentakisphosphate and inositol hexakisphosphate levels