Information on EC 2.7.1.14 - sedoheptulokinase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.7.1.14
-
RECOMMENDED NAME
GeneOntology No.
sedoheptulokinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + sedoheptulose = ADP + sedoheptulose 7-phosphate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Carbon fixation in photosynthetic organisms
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-
SYSTEMATIC NAME
IUBMB Comments
ATP:sedoheptulose 7-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9030-63-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain W-2
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Manually annotated by BRENDA team
strain W-2
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
cystinosis is caused by mutations in CTNS. The most prevalent CTNS mutation is a homozygous 57-kb deletion. This 57-kb deletion encompasses not only the CTNS gene but also the adjacent gene, CARKL. Patients with this deletion have elevated urinary concentrations of sedoheptulose
physiological function
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using a screening strategy novel regulators of macrophage activation are identified. CARKL-dependent metabolic reprogramming is required for proper M1- and M2- like macrophage polarization and uncovers a ratelimiting requirement for appropriate glucose flux in macrophage polarization
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + altrose
?
show the reaction diagram
weak activity
-
-
?
ATP + mannoheptulose
?
show the reaction diagram
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-
-
?
ATP + sedoheptulose
ADP + sedoheptulose 7-phosphate
show the reaction diagram
ATP + sedoheptulose
ADP + sedoheptulose monophosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + sedoheptulose
ADP + sedoheptulose monophosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required, optimum concentration: 0.01 M
Mn2+
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can partially replace Mg2+ in activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Alloxane
-
inhibits above 1 mM, activity restored if cysteine is added at the same concentration
CuSO4
-
95% inhibition at 10 mM
iodoacetate
-
60% inhibition at 10 mM
NaF
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28% inhibition at 1 mM
NaN3
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80% inhibition at 10 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.19
sedoheptulose
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
115
sedoheptulose
Mus musculus
Q9D5J6
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4
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and a smaller maximum at pH 9.0
7.6
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assay at
9
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and a larger maximum at pH 6.4
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
35
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli as a N-terminal fusion protein with a polyHis tag
recombinantly expressed in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
rapidly downregulated in vitro and in vivo upon lipopolysaccharide
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Q126K
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mutant shows substantially reduced sedoheptulose kinase activity
T14M/T15K
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mutant shows substantially reduced sedoheptulose kinase activity
W125D
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mutant shows substantially reduced sedoheptulose kinase activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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sedoheptulokinase deficiency due to a 57-kb deletion in cystinosis patients causes urinary accumulation of sedoheptulose