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ATP + D-gluconate
ADP + 6-phospho-D-gluconate
ATP + D-glucono-1,5-lactone
ADP + 6-phospho-D-glucono-1,5-lactone
-
-
-
?
CTP + D-gluconate
CDP + 6-phospho-D-gluconate
-
-
-
?
D-gluconate + ATP
6-phospho-D-gluconate + ADP
GTP + D-gluconate
GDP + 6-phospho-D-gluconate
ITP + D-gluconate
IDP + 6-phospho-D-gluconate
-
60% of the activity with ATP
-
-
?
UTP + D-gluconate
UDP + 6-phospho-D-gluconate
additional information
?
-
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
enzyme probably acts as a key enzyme in regulating glucose oxidation in the liver of fish
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
enzyme probably acts as a key enzyme in regulating glucose oxidation in the liver of fish
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
enzyme of the oxidative pathway in glucose-grown bacteria
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
enzyme of the oxidative pathway in glucose-grown bacteria
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
enzyme probably acts as a key enzyme in regulating glucose oxidation in the liver of fish
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
strict specificity for D-gluconate
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
and ATP
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
conversion of glucose to pyruvate
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
6-phosphogluconate dehydrogenase (Gcd1) and gluconate kinase (Idn1) act together to shunt glucose into the pentose phosphate pathway, creating an alternative route for directing glucose into the pentose phosphate pathway that bypasses hexokinase and the rate-limiting enzyme glucose-6-phosphate dehydrogenase
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
6-phosphogluconate dehydrogenase (Gcd1) and gluconate kinase (Idn1) act together to shunt glucose into the pentose phosphate pathway, creating an alternative route for directing glucose into the pentose phosphate pathway that bypasses hexokinase and the rate-limiting enzyme glucose-6-phosphate dehydrogenase
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
enzyme probably acts as a key enzyme in regulating glucose oxidation in the liver of fish
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
strict specificity for D-gluconate
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
D-gluconate + ATP
6-phospho-D-gluconate + ADP
-
-
-
-
?
D-gluconate + ATP
6-phospho-D-gluconate + ADP
-
-
-
-
?
D-gluconate + ATP
6-phospho-D-gluconate + ADP
-
-
-
?
GTP + D-gluconate
GDP + 6-phospho-D-gluconate
-
-
-
?
GTP + D-gluconate
GDP + 6-phospho-D-gluconate
-
25% of the activity with ATP
-
-
?
UTP + D-gluconate
UDP + 6-phospho-D-gluconate
-
-
-
?
UTP + D-gluconate
UDP + 6-phospho-D-gluconate
-
130% of the activity with ATP
-
-
?
additional information
?
-
no substrates: D-glucose, D-galactose, D-fructose, D-mannose and D-glucosamine, D-ribose, D-ribulose, D-arabinose, D-xylulose, D-xylose and D-erythrose
-
-
?
additional information
?
-
-
no substrates: D-glucose, D-galactose, D-fructose, D-mannose and D-glucosamine, D-ribose, D-ribulose, D-arabinose, D-xylulose, D-xylose and D-erythrose
-
-
?
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ATP + D-gluconate
ADP + 6-phospho-D-gluconate
D-gluconate + ATP
6-phospho-D-gluconate + ADP
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
enzyme probably acts as a key enzyme in regulating glucose oxidation in the liver of fish
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
enzyme probably acts as a key enzyme in regulating glucose oxidation in the liver of fish
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
enzyme of the oxidative pathway in glucose-grown bacteria
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
enzyme of the oxidative pathway in glucose-grown bacteria
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
enzyme probably acts as a key enzyme in regulating glucose oxidation in the liver of fish
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
conversion of glucose to pyruvate
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
6-phosphogluconate dehydrogenase (Gcd1) and gluconate kinase (Idn1) act together to shunt glucose into the pentose phosphate pathway, creating an alternative route for directing glucose into the pentose phosphate pathway that bypasses hexokinase and the rate-limiting enzyme glucose-6-phosphate dehydrogenase
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
6-phosphogluconate dehydrogenase (Gcd1) and gluconate kinase (Idn1) act together to shunt glucose into the pentose phosphate pathway, creating an alternative route for directing glucose into the pentose phosphate pathway that bypasses hexokinase and the rate-limiting enzyme glucose-6-phosphate dehydrogenase
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
enzyme probably acts as a key enzyme in regulating glucose oxidation in the liver of fish
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
-
-
-
-
?
D-gluconate + ATP
6-phospho-D-gluconate + ADP
-
-
-
-
?
D-gluconate + ATP
6-phospho-D-gluconate + ADP
-
-
-
-
?
D-gluconate + ATP
6-phospho-D-gluconate + ADP
-
-
-
?
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additional information
additional information
-
0.045
ATP
-
-
0.344
ATP
pH 7.2, temperature not specified in the publication
0.02
D-gluconate
-
-
0.042
D-gluconate
-
25°C, pH 7
0.106
D-gluconate
pH 7.2, temperature not specified in the publication
0.212
D-gluconate
-
25°C, pH 8, gene gntU
1.74
D-gluconate
-
30°C, pH 7.6
additional information
additional information
-
30°C, 0.34-4.1 mM with D-gluconate as substrate, depending on organism
-
additional information
additional information
-
30°C, 0.34-4.1 mM with D-gluconate as substrate, depending on organism
-
additional information
additional information
-
30°C, 0.34-4.1 mM with D-gluconate as substrate, depending on organism
-
additional information
additional information
-
30°C, 0.34-4.1 mM with D-gluconate as substrate, depending on organism
-
additional information
additional information
-
method for quantification of D-gluconate by use of immobilized enzyme
-
additional information
additional information
isothermal titration calorimetry to determine kinetic and thermodynamic properties. The KM for ATP lies in the range 0.1 to 0.3 mM at varying concentrations of Glcn. The KM of D-glucuronate is in the range 0.2 to 0.3 mM, pH 7.2, 25°C
-
additional information
additional information
-
isothermal titration calorimetry to determine kinetic and thermodynamic properties. The KM for ATP lies in the range 0.1 to 0.3 mM at varying concentrations of Glcn. The KM of D-glucuronate is in the range 0.2 to 0.3 mM, pH 7.2, 25°C
-
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0.00035
-
strain TUG287(pTC222)
0.00621
-
strain TUG287(pTC223)
0.047
activity of glucokinase in Corynebacterium glutamicum (ATCC 13032) culture in MCGC medium at 37°C, supplemented with 0.5% gluconate
0.388
activity of glucokinase expressed in Escherichia strain M1-8, supplemented with fructose as carbon source
0.6725
-
strain TUG287(pTC220)
1.429
transformed Escherichia coli cells (strain DH5alpha carrying the pTAEK plasmid), grown in Lb and collected during the exponential phase, then cultivated in CAA medium with 0.2% fructose as carbon source
2.894
transformed Escherichia coli cells (mutant TGN282 carrying the pTAEK plasmid), grown in Lb and collected during the exponential phase, then cultivated in CAA medium with 0.2% fructose as carbon source
250
-
thermoresistant gluconokinase encoded by th gntK
additional information
-
at six concentrations (4.6-90.6 mM) of gluconate added to culture medium for Azospirillum brasilense Cd grown in TYG medium, activity of gluconokinase activity is positively affected
additional information
-
gluconokinase activity significantly increases in inoculated mesquite plants incubated for 23 days, inoculation is not enhanced after 15 days of incubation
additional information
-
while increased gluconokinase activity results only in inoculated treatments, older non-inoculated seedlings maintained the same activity as younger seedlings and the sum of activities of the roots and the negative control is still lower than activity in inoculated plants
additional information
-
35.1 microliter of CO2 per h per mg of protein
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drug target
gluconokinase IDNK plays a key role in the proliferation and apoptosis of HCC cells. It may be a candidate therapeutic target for hepatocellular carcinoma
malfunction
-
inactivation of the gluconate kinase gene leads to a 51.8% increase in intracellular NADPH concentration, which results in a 49.9% increase in L-ornithine production
metabolism
pentose phosphate pathway
metabolism
-
phosphogluconate pathway, bacterial enzyme is active in young, non-inoculated mesquite seedlings growing under hydroponic conditions
metabolism
6-phosphogluconate dehydrogenase (Gcd1) and gluconate kinase (Idn1) act together to shunt glucose into the pentose phosphate pathway, creating an alternative route for directing glucose into the pentose phosphate pathway that bypasses hexokinase and the rate-limiting enzyme glucose-6-phosphate dehydrogenase
metabolism
-
phosphogluconate pathway, bacterial enzyme is active in young, non-inoculated mesquite seedlings growing under hydroponic conditions
-
metabolism
-
6-phosphogluconate dehydrogenase (Gcd1) and gluconate kinase (Idn1) act together to shunt glucose into the pentose phosphate pathway, creating an alternative route for directing glucose into the pentose phosphate pathway that bypasses hexokinase and the rate-limiting enzyme glucose-6-phosphate dehydrogenase
-
physiological function
-
when azospirillum brasilense Cd bacteria are inoculated on the root system, the roots exhibit much higher activity of gluconokinase
physiological function
gluconokinase IDNK promotes cell proliferation and inhibits apoptosis in hepatocellular carcinoma
physiological function
-
when azospirillum brasilense Cd bacteria are inoculated on the root system, the roots exhibit much higher activity of gluconokinase
-
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Zachariou, M.; Scopes, R.K.
Gluconate kinase from Zymomonas mobilis: isolation and characteristics
Biochem. Int.
10
367-371
1985
Zymomonas mobilis
brenda
Nagayama, F.; Suzuki, H.; Oshima, T.
Gluconokinase of fish liver
Nippon Suisan Gakkaishi
46
783
1980
Anguilla japonica, Cyprinus carpio, Oncorhynchus mykiss, Seriola quinqueradiata
-
brenda
Szymona, M.; Kowalska, H.
ATP: D-gluconate 6-phosphotransferase of Mycobacterium phlei
Ann. Univ. Mariae Curie Sklodowska Med.
25
371-381
1970
Mycolicibacterium phlei
brenda
Cohen, S.S.
Gluconokinase and the oxidative path of glucose-6-phosphate utilization
J. Biol. Chem.
189
617-628
1951
Escherichia coli, Escherichia coli B / ATCC 11303
brenda
Sable, H.Z.; Guarino, A.J.
Phosphorylation of gluconate in yeast extracts
J. Biol. Chem.
196
395-402
1952
Saccharomyces cerevisiae
brenda
Narrod, S.A.; Wood, W.A.
Carbohydrate oxidation by pseudomonas fluorescens. V. Evidence for gluconokinase and 2-ketogluconokinase
J. Biol. Chem.
220
45-55
1956
Pseudomonas fluorescens
brenda
Leder, I.G.
Hog kidney gluconokinase
J. Biol. Chem.
225
125-136
1957
Sus scrofa
brenda
Coffee, C.J.; Hu, A.S.L.
The kinetic characterization of gluconokinase from a pseudomonad
Arch. Biochem. Biophys.
149
549-559
1972
Pseudomonas sp.
brenda
Tong, S.; Porco, A.; Isturiz, T.; Conway, T.
Cloning and molecular genetic characterization of the Escherichia coli gntR, gntK, and gntU genes of GntI, the main system for gluconate metabolism
J. Bacteriol.
178
3260-3269
1996
Escherichia coli
brenda
Izu, H.; Adachi, O.; Yamada, M.
Purification and characterization of the Escherichia coli thermoresistant glucokinase encoded by the gntK gene
FEBS Lett.
394
14-16
1996
Escherichia coli
brenda
Kraft, L.; Sprenger, G.A.; Lindqvist, Y.
Crystallization and preliminary X-ray crystallographic studies of recombinant thermoresistant gluconate kinase GntK from Escherichia coli
Acta Crystallogr. Sect. D
57
1159-1161
2001
Escherichia coli
brenda
Kraft, L.; Sprenger, G.A.; Lindqvist, Y.
Conformational changes during the catalytic cycle of gluconate kinase as revealed by X-ray crystallography
J. Mol. Biol.
318
1057-1069
2002
Escherichia coli
brenda
Tsukatani, T.; Matsumoto, K.
Fluorometric quantification of total D-gluconate by a flow-injection system using an immobilized-enzyme reactor
Anal. Chim. Acta
530
221-225
2005
Escherichia coli
-
brenda
Porco, A.; Gamero, E.E.; Mylonas, E.; Isturiz, T.
Gluconate as suitable potential reduction supplier in Corynebacterium glutamicum: cloning and expression of gntP and gntK in Escherichia coli
Biol. Res.
41
349-358
2008
Corynebacterium glutamicum (Q8NMT0), Corynebacterium glutamicum
brenda
Leyva, L.A.; Bashan, Y.
Activity of two catabolic enzymes of the phosphogluconate pathway in mesquite roots inoculated with Azospirillum brasilense Cd
Plant Physiol. Biochem.
46
898-904
2008
Azospirillum brasilense, Azospirillum brasilense Cd
brenda
Rolfsson, O.; Paglia, G.; Magnusdottir, M.; Palsson, B.O.; Thiele, I.
Inferring the metabolism of human orphan metabolites from their metabolic network context affirms human gluconokinase activity
Biochem. J.
449
427-435
2013
Homo sapiens (Q5T6J7), Homo sapiens
brenda
Hwang, G.H.; Cho, J.Y.
Implication of gluconate kinase activity in L-ornithine biosynthesis in Corynebacterium glutamicum
J. Ind. Microbiol. Biotechnol.
39
1869-1874
2012
Corynebacterium glutamicum
brenda
Rohatgi, N.; Gudmundsson, S.; Rolfsson, O.
Kinetic analysis of gluconate phosphorylation by human gluconokinase using isothermal titration calorimetry
FEBS Lett.
589
3548-3555
2015
Homo sapiens (Q5T6J7), Homo sapiens
brenda
Rohatgi, N.; Nielsen, T.; Bjorn, S.; Axelsson, I.; Paglia, G.; Voldborg, B.; Palsson, B.; Rolfsson, O.
Biochemical characterization of human gluconokinase and the proposed metabolic impact of gluconic acid as determined by constraint based metabolic network analysis
PLoS ONE
9
e98760
2014
Homo sapiens (Q5T6J7), Homo sapiens
brenda
Corkins, M.E.; Wilson, S.; Cocuron, J.C.; Alonso, A.P.; Bird, A.J.
The gluconate shunt is an alternative route for directing glucose into the pentose phosphate pathway in fission yeast
J. Biol. Chem.
292
13823-13832
2017
Schizosaccharomyces pombe (Q10242), Schizosaccharomyces pombe, Schizosaccharomyces pombe ATCC 24843 (Q10242)
brenda
Wu, X.M.; Jin, C.; Gu, Y.L.; Chen, W.Q.; Zhu, M.Q.; Zhang, S.; Zhang, Z.
Gluconokinase IDNK promotes cell proliferation and inhibits aoptosis in hepatocellular carcinoma
OncoTargets Ther.
13
1767-1776
2020
Homo sapiens (Q5T6J7)
brenda