Information on EC 2.7.1.12 - gluconokinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.1.12
-
RECOMMENDED NAME
GeneOntology No.
gluconokinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + D-gluconate = ADP + 6-phospho-D-gluconate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-gluconate degradation
-
-
L-idonate degradation
-
-
sorbitol biosynthesis II
-
-
ketogluconate metabolism
-
-
Pentose phosphate pathway
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
-
Microbial metabolism in diverse environments
-
-
Biosynthesis of antibiotics
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:D-gluconate 6-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9030-55-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
eel
-
-
Manually annotated by BRENDA team
carp
-
-
Manually annotated by BRENDA team
isoform 1; isoform 2
UniProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
rainbow trout
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
baker's yeast and brewer's yeast
-
-
Manually annotated by BRENDA team
yellowtail
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
inactivation of the gluconate kinase gene leads to a 51.8% increase in intracellular NADPH concentration, which results in a 49.9% increase in L-ornithine production
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
show the reaction diagram
ATP + myelin basic protein kinase
ADP + phosphorylated myelin basic protein kinase
show the reaction diagram
-
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
autophosphorylation
-
-
?
D-gluconate + ATP
6-phospho-D-gluconate + ADP
show the reaction diagram
GTP + D-gluconate
GDP + 6-phospho-D-gluconate
show the reaction diagram
-
25% of the activity with ATP
-
-
?
ITP + D-gluconate
IDP + 6-phospho-D-gluconate
show the reaction diagram
-
60% of the activity with ATP
-
-
?
UTP + D-gluconate
UDP + 6-phospho-D-gluconate
show the reaction diagram
-
130% of the activity with ATP
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-gluconate
ADP + 6-phospho-D-gluconate
show the reaction diagram
D-gluconate + ATP
6-phospho-D-gluconate + ADP
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
can partially replace Mg2+ in activation
Co2+
-
can partially replace Mg2+ in activation
MgCl2
-
10 mM are included in assay medium
Zn2+
-
can partially replace Mg2+ in activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
ATP protects
dADP
-
weak
IDP
-
weak
p-chloromercuribenzoate
-
-
additional information
-
not: iodoacetate
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
activated His-tagged Cdc42Hs
stimulates mPAK-3 autophosphorylation and phosphorylation of myelin basic protein by mPAK-3 in vitro
-
gluconate
6-phosphogluconate dehydrogenase, although constituvely expressed, shows a 3fold higher specific level in gluconate grown cells than those grown in fructose under similar conditions
gluconic acid
-
gluconokinase is induced upon exposure to gluconic acid
His-tagged Rac
stimulates mPAK-3 autophosphorylation and phosphorylation of myelin basic protein by mPAK-3 in vitro
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.045 - 1.04
ATP
0.02 - 1.74
D-gluconate
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00035
-
strain TUG287(pTC222)
0.00088
-
strain TUG287
0.00621
-
strain TUG287(pTC223)
0.047
activity of glucokinase in Corynebacterium glutamicum (ATCC 13032) culture in MCGC medium at 37C, supplemented with 0.5% gluconate
0.388
activity of glucokinase expressed in Escherichia strain M1-8, supplemented with fructose as carbon source
0.64
-
-
0.6547
-
strain W1485
0.6725
-
strain TUG287(pTC220)
1.429
transformed Escherichia coli cells (strain DH5alpha carrying the pTAEK plasmid), grown in Lb and collected during the exponential phase, then cultivated in CAA medium with 0.2% fructose as carbon source
2.894
transformed Escherichia coli cells (mutant TGN282 carrying the pTAEK plasmid), grown in Lb and collected during the exponential phase, then cultivated in CAA medium with 0.2% fructose as carbon source
250
-
thermoresistant gluconokinase encoded by th gntK
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2
-
-
7.8 - 8.2
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.1 - 8.4
-
pH 5.1: about 25% of activity maximum, pH 8.4: about 50% of activity maximum
5.4 - 6.8
6 - 9
-
pH 6: about 30% of activity maximum, pH 9: about 40% of activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
assay at
30
-
assay at
34
-
assay at
45
-
thermoresistant gluconokinase encoded by th gntK
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Rhizobium meliloti (strain 1021)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
monomer
-
1 * 18000
trimer
-
alpha3, 3 * 29500
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
autophosphorylation
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour-diffusion method with polyethylene glycol 6000 and lithium chloride as precipitants, three crystal forms obtained, space group C2 with a : 75, b : 79.3, c : 70.2 A and beta : 105.3, space group P212121 with a : 52, b : 79.3, c : 89.8 A and space group P21212 with a : 70.1, b : 74.1 and c : 78.9 A
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
after 40 min, 50% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15C, 10% loss of activity after 3 weeks
-
3C, 50% loss of activity after 3 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
baker's yeast and brewer's yeast
-
partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in in human HeLa cell lysates
expression in Escherichia coli Hfr6GDELTAMD2, TUG287 and M6
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
glucokinase is induced upon exposure to gluconic acid
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nutrition
-
method for quantification of D-gluconate in honeys, vinegars and noble rot wines by use of immobilized enzyme
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