Information on EC 2.7.1.114 - AMP-thymidine kinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.114
-
RECOMMENDED NAME
GeneOntology No.
AMP-thymidine kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
AMP + thymidine = adenosine + dTMP
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
AMP:thymidine 5'-phosphotransferase
The deoxypyrimidine kinase complex induced by Herpes simplex virus catalyses this reaction as well as those of EC 2.7.1.21 (thymidine kinase), EC 2.7.1.118 (ADP---thymidine kinase) and EC 2.7.4.9 (dTMP kinase).
CAS REGISTRY NUMBER
COMMENTARY hide
60440-28-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bird's nest fern
-
-
Manually annotated by BRENDA team
Epidendrum hybrid
crucifix orchid
-
-
Manually annotated by BRENDA team
Jerusalem artichoke
-
-
Manually annotated by BRENDA team
Herpes simplex virus
barley
-
-
Manually annotated by BRENDA team
lucerne
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
AMP + 5-bromodeoxyuridine
adenosine + 5-bromodeoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
-
?
AMP + adenosine
adenosine + AMP
show the reaction diagram
-
-
-
-
?
AMP + cytidine
adenosine + cytidine 5'-phosphate
show the reaction diagram
-
-
-
-
?
AMP + deoxyuridine
adenosine + deoxyuridine 5'-phosphate
show the reaction diagram
-
-
-
-
?
AMP + guanosine
adenosine + guanosine 5'-phosphate
show the reaction diagram
-
-
-
-
?
AMP + thymidine
adenosine + dTMP
show the reaction diagram
AMP + uridine
adenosine + uridine 5'-phosphate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
AMP + thymidine
adenosine + dTMP
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
3',5'-cAMP
-
-
9-beta-D-Arabinofuranosyladenine 5'-monophosphate
Herpes simplex virus
-
-
adenosine
-
-
uridine
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.28
adenosine
-
38°C, pH 7.5
0.0023 - 0.11
AMP
0.0021 - 0.88
thymidine
4.71
uridine
-
38°C, pH 7.5
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.002
-
leaf
0.06
-
tops
0.07
-
senescent leaf
0.29
-
mature leaf
0.47
-
young and expanded leaf
0.84
-
mature leaf
0.91
-
young mature leaf
1.09
-
immature leaf
1.64
-
young bud
42.2
Herpes simplex virus
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6
Herpes simplex virus
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3 - 8.8
Herpes simplex virus
-
17% of maximal activity at pH 6.3, 32% of maximal activity at pH 8.8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37 - 38
Herpes simplex virus
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90600 - 111000
Herpes simplex virus
-
glycerol density gradient centrifugation
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
60 min, 95% loss of activity
63
-
5 min, complete inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
bovine serum albumin stabilizes
-
not stabilized by AMP or thymidine
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, 6 months, 20% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
type 1, hamster BHK-cells infected with virus
Herpes simplex virus
-